SSGCID: Target Page

RicoA.00893.b

Thioredoxin reductase

CENTER ID:	RicoA.00893.b
ORGANISM:	Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE:	Boutonneuse fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RicoA.00893.b.B2.GE35283	Mature protein( RicoA.00893.b )		26	310

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272944.4.peg.703
RefSeq:	NP_360255.1
UniProt:	Q92I02

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKITTKVLII GSGPAGLSAA IYTARAALKP ILINGMQPGG QLTITTDVEN YPGFAETVQG PWLMEQMYMQ AKNVGTEIVS DYVEKVDLSK RPFKVFTGAG NEYDAESIII CTGAEAKWLG IASEQKFRGF GVSACATCDG FFFKNQEIVV VGGGNSAVEE ALYLTNHANK VTIVHRRDSF RAEKILQDRL FKNSKISVIW DHVVDEIVGS NKPKSVTGVK IQNVHTKEIS LLNCSGVFIA IGHAPNTGLF TGQIVMDDDN YIITKSGTTR TSVEGVFAAG DVQDKIYRQA VTAAGTGCMA ALEAEKFLNK

NT Sequence

ATGAAAATTA CTACTAAAGT ACTAATAATA GGTTCAGGAC CTGCGGGCTT AAGTGCCGCT ATTTATACAG CTAGAGCTGC TTTAAAACCA ATATTAATAA ATGGTATGCA GCCAGGCGGG CAGCTTACAA TTACCACCGA TGTTGAGAAC TATCCAGGAT TTGCGGAAAC GGTGCAAGGA CCTTGGCTTA TGGAGCAGAT GTATATGCAG GCTAAAAATG TCGGTACGGA AATTGTTAGT GATTACGTAG AAAAGGTGGA TTTATCAAAA AGACCTTTTA AAGTATTTAC AGGAGCAGGA AATGAATATG ACGCGGAGAG CATAATCATT TGTACCGGTG CGGAAGCAAA ATGGCTTGGT ATAGCTTCAG AGCAGAAATT TCGTGGTTTT GGTGTTTCGG CATGTGCTAC TTGCGATGGT TTCTTTTTTA AGAATCAAGA AATAGTTGTA GTAGGGGGAG GAAATAGTGC AGTAGAAGAA GCTTTATACT TAACCAATCA TGCTAACAAA GTCACTATAG TGCATAGAAG AGATAGTTTT AGAGCTGAGA AAATATTACA GGATCGATTA TTTAAAAATT CTAAAATATC AGTAATTTGG GATCATGTAG TAGATGAGAT TGTAGGTAGC AATAAACCAA AATCCGTTAC TGGAGTTAAA ATTCAAAATG TTCACACCAA AGAGATTAGT TTATTGAATT GTAGTGGTGT ATTTATAGCT ATTGGGCATG CACCGAATAC GGGGCTATTT ACCGGACAAA TTGTAATGGA TGACGATAAT TATATCATCA CGAAGTCAGG TACTACTAGA ACTAGTGTAG AAGGTGTTTT TGCTGCTGGT GACGTTCAGG ATAAAATTTA TAGACAAGCA GTAACTGCTG CAGGAACAGG CTGTATGGCG GCTCTTGAGG CTGAAAAATT TCTAAATAAA

Details for RicoA.00893.b.B2.GE35283

HARVESTED ON:	5/1/2012
SEQUENCED ON:	5/2/2012
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA ALKPILINGM QPGGQLTITT DVENYPGFAE TVQGPWLMEQ MYMQAKNVGT EIVSDYVEKV DLSKRPFKVF TGAGNEYDAE SIIICTGAEA KWLGIASEQK FRGFGVSACA TCDGFFFKNQ EIVVVGGGNS AVEEALYLTN HANKVTIVHR RDSFRAEKIL QDRLFKNSKI SVIWDHVVDE IVGSNKPKSV TGVKIQNVHT KEISLLNCSG VFIAIGHAPN TGLFTGQIVM DDDNYIITKS GTTRTSVEGV FAAGDVQDKI YRQAVTAAGT GCMAALEAEK FLNK

Validated NT Sequence

attttnttta ctttaagaag gaganatacc atggctcacc accaccacca ccatatggct gctttaaaac caatattaat aaatggtatg cagccaggcg ggcagcttac aattaccacc gatgttgaga actatccagg atttgcggaa acggtgcaag gaccttggct tatggagcag atgtatatgc aggctaaaaa tgtcggtacg gaaattgtta gtgattacgt agaaaaggtg gatttatcaa aaagaccttt taaagtattt acaggagcag gaaatgaata tgacgcggag agcataatca tttgtaccgg tgcggaagca aaatggcttg gtatagcttc agagcagaaa tttcgtggtt ttggtgtttc ggcatgtgct acttgcgatg gtttcttttt taagaatcaa gaaatagttg tagtaggggg aggaaatagt gcagtagaag aagctttata cttaaccaat catgctaaca aagtcactat agtgcataga agagatagtt ttagagctga gaaaatatta caggatcgat tatttaaaaa ttctaaaata tcagtaattt gggatcatgt agtagatgag attgtaggta gcaataaacc aaaatccgtt actggagtta aaattcaaaa tgttcacacc aaagagatta gtttattgaa ttgtagtggt gtatttatag ctattgggca tgcaccgaat acggggctat ttaccggaca aattgtaatg gatgacgata attatatcat cacgaagtca ggtactacta gaactagtgt agaaggtgtt tttgctgctg gtgacgttca ggataaaatt tatagacaag cagtaactgc tgcaggaaca ggctgtatgg cggctcttga ggctgaaaaa tttctaaata aataagtgag taanatagga ncnnnnnnnn nncccnnnnn nnnnnnnnnn nnnnnnnnan nnnnnnnnna aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnannnnnn tatnnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctg ctttaaaacc aatattaata aatggtatgc agccaggcgg gcagcttaca attaccaccg atgttgagaa ctatccagga tttgcggaaa cggtgcaagg accttggctt atggagcaga tgtatatgca ggctaaaaat gtcggtacgg aaattgttag tgattacgta gaaaaggtgg atttatcaaa aagacctttt aaagtattta caggagcagg aaatgaatat gacgcggaga gcataatcat ttgtaccggt gcggaagcaa aatggcttgg tatagcttca gagcagaaat ttcgtggttt tggtgtttcg gcatgtgcta cttgcgatgg tttctttttt aagaatcaag aaatagttgt agtaggggga ggaaatagtg cagtagaaga agctttatac ttaaccaatc atgctaacaa agtcactata gtgcatagaa gagatagttt tagagctgag aaaatattac aggatcgatt atttaaaaat tctaaaatat cagtaatttg ggatcatgta gtagatgaga ttgtaggtag caataaacca aaatccgtta ctggagttaa aattcaaaat gttcacacca aagagattag tttattgaat tgtagtggtg tatttatagc tattgggcat gcaccgaata cggggctatt taccggacaa attgtaatgg atgacgataa ttatatcatc acgaagtcag gtactactag aactagtgta gaaggtgttt ttgctgctgg tgacgttcag gataaaattt atagacaagc agtaactgct gcaggaacag gctgtatggc ggctcttgag gctgaaaaat ttctaaataa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat