RicoA.00893.a

Ferredoxin--NADP reductase

CENTER ID: RicoA.00893.a
ORGANISM: Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE: Boutonneuse fever
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.00893.a.B1.GE35282 Full length( RicoA.00893.a ) 1 340
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.00893.a.B1.PW35900 Full length( RicoA.00893.a ) 1 340

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272944.4.peg.725
RefSeq: NP_360274.1
UniProt: Q92HY3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYNTDVVIIG AGPVGLFAVF QAGMLGMKCH VIDAQEVIGG QCITLYPEKP IYDIPAYPKI AAEELIKQLA LQAAPFNPIY HLNQQAIELN KQDDFFEIKT SKNTLIKSKV IIIAAGAGSF GPNKPPLANI EDFESKSVFY FINDKSKFAG KNIVIAGGGD SAVDWAISLS DIANKIYLVH RRDKFTAAPE SVRQLRHIAE TDKIELITGY QLNALDGNNS ELQSVIVKDL QNNTRKLDAN ILLPFFGLKQ DLGSLANWGL NVKLHHIEVD SSYYQTNIEG IYAIGDIAHY VGKLKLILTG FAEAASSLHH AYSRVFDGKA LHFEYSTTKN TGKRSKSVTK
NT Sequence
ATGTATAATA CTGATGTTGT AATAATAGGT GCCGGTCCGG TTGGATTATT TGCAGTTTTT CAAGCAGGAA TGCTTGGCAT GAAGTGTCAT GTTATTGATG CACAAGAAGT TATCGGCGGT CAGTGTATTA CCCTATATCC TGAAAAGCCT ATTTACGATA TACCAGCATA TCCTAAAATT GCAGCAGAAG AGCTAATAAA ACAGTTAGCA CTTCAAGCAG CTCCTTTTAA CCCTATATAT CATTTAAATC AGCAAGCTAT AGAGCTAAAT AAACAAGATG ATTTTTTTGA AATTAAAACC TCAAAAAACA CTCTTATTAA AAGCAAAGTT ATTATTATTG CAGCAGGAGC AGGTTCATTC GGTCCAAACA AACCGCCCCT TGCGAATATT GAAGATTTTG AAAGCAAGTC AGTTTTTTAT TTCATCAATG ATAAAAGCAA ATTTGCGGGT AAAAATATAG TTATAGCAGG CGGCGGGGAT TCGGCTGTTG ACTGGGCTAT TTCTCTTTCA GATATTGCAA ATAAAATATA TTTAGTACAT AGGCGGGATA AATTTACGGC AGCTCCTGAA AGCGTAAGGC AATTAAGACA TATTGCAGAA ACCGATAAAA TCGAGTTAAT AACCGGTTAT CAATTAAATG CTTTAGATGG TAATAATAGT GAACTTCAAT CGGTTATAGT TAAAGATTTA CAAAATAATA CTCGCAAATT GGATGCCAAT ATATTACTAC CTTTTTTTGG TTTAAAACAA GATTTAGGTA GCTTAGCAAA TTGGGGTTTA AATGTTAAGC TTCACCATAT TGAAGTAGAC TCTTCCTATT ATCAAACTAA TATAGAAGGT ATTTACGCCA TTGGCGATAT TGCTCATTAT GTTGGTAAGC TTAAGCTGAT TCTTACGGGT TTTGCTGAAG CGGCAAGCAG TCTTCATCAT GCTTATAGTA GAGTATTTGA CGGCAAGGCT CTACATTTTG AGTATTCTAC TACCAAAAAT ACGGGGAAAA GAAGTAAGAG CGTGACTAAA
Details for RicoA.00893.a.B1.GE35282
HARVESTED ON: 5/1/2012
SEQUENCED ON: 5/2/2012
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMY NTDVVIIGAG PVGLFAVFQA GMLGMKCHVI DAQEVIGGQC ITLYPEKPIY DIPAYPKIAA EELIKQLALQ AAPFNPIYHL NQQAIELNKQ DDFFEIKTSK NTLIKSKVII IAAGAGSFGP NKPPLANIED FESKSVFYFI NDKSKFAGKN IVIAGGGDSA VDWAISLSDI ANKIYLVHRR DKFTAAPESV RQLRHIAETD KIELITGYQL NALDGNNSEL QSVIVKDLQN NTRKLDANIL LPFFGLKQDL GSLANWGLNV KLHHIEVDSS YYQTNIEGIY AIGDIAHYVG KLKLILTGFA EAASSLHHAY SRVFDGKALH FEYSTTKNTG KRSKS
Validated NT Sequence
cacgctctta cttcttttcc ccgtattttt ggtagtagaa tactcaaaat gtagagcctt gccgtcaaat actctactat aagcatgatg aagactgctt gccgcttcag caaaacccgt aagaatcagc ttaagcttac caacataatg agcaatatcg ccaatggcgt aaataccttc tatattagtt tgataatagg aagagtctac ttcaatatgg tgaagcttaa catttaaacc ccaatttgct aagctaccta aatcttgttt taaaccaaaa aaaggtagta atatattggc atccaatttg cgagtattat tttgtaaatc tttaactata accgattgaa gttcactatt attaccatct aaagcattta attgataacc ggttattaac tcgattttat cggtttctgc aatatgtctt aattgcctta cgctttcagg agctgccgta aatttatccc gcctatgtac taaatatatt ttatttgcaa tatctgaaag agaaatagcc cagtcaacag ccgaatcccc gccgcctgct ataactatat ttttacccgc aaatttgctt ttatcattga tgaaataaaa aactgacttg ctttcaaaat cttcaatatt cgcaaggggc ggtttgtttg gaccgaatga acctgctcct gctgcaataa taataacttt gcttttaata agagtgtttt ttgaggtttt aatttcaaaa aaatcatctt gtttatttag ctctatagct tgctgattta aatgatatat agggttaaaa ggagctgctt gaagtgctaa ctgttttatt agctcttctg ctgcaatttt aggatatgct ggtatatcgt aaataggctt ttcaggatat agggtaatac actgaccgcc gataacttct tgtgcatcaa taacatgaca cttcatgcca agcattcctg cttgaaaaac tgcaaataat ccaaccggac cggcacctat tattacaaca tcagtattat acatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMY NTDVVIIGAG PVGLFAVFQA GMLGMKCHVI DAQEVIGGQC ITLYPEKPIY DIPAYPKIAA EELIKQLALQ AAPFNPIYHL NQQAIELNKQ DDFFEIKTSK NTLIKSKVII IAAGAGSFGP NKPPLANIED FESKSVFYFI NDKSKFAGKN IVIAGGGDSA VDWAISLSDI ANKIYLVHRR DKFTAAPESV RQLRHIAETD KIELITGYQL NALDGNNSEL QSVIVKDLQN NTRKLDANIL LPFFGLKQDL GSLANWGLNV KLHHIEVDSS YYQTNIEGIY AIGDIAHYVG KLKLILTGFA EAASSLHHAY SRVFDGKALH FEYSTTKNTG KRSKSVTK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtata atactgatgt tgtaataata ggtgccggtc cggttggatt atttgcagtt tttcaagcag gaatgcttgg catgaagtgt catgttattg atgcacaaga agttatcggc ggtcagtgta ttaccctata tcctgaaaag cctatttacg atataccagc atatcctaaa attgcagcag aagagctaat aaaacagtta gcacttcaag cagctccttt taaccctata tatcatttaa atcagcaagc tatagagcta aataaacaag atgatttttt tgaaattaaa acctcaaaaa acactcttat taaaagcaaa gttattatta ttgcagcagg agcaggttca ttcggtccaa acaaaccgcc ccttgcgaat attgaagatt ttgaaagcaa gtcagttttt tatttcatca atgataaaag caaatttgcg ggtaaaaata tagttatagc aggcggcggg gattcggctg ttgactgggc tatttctctt tcagatattg caaataaaat atatttagta cataggcggg ataaatttac ggcagctcct gaaagcgtaa ggcaattaag acatattgca gaaaccgata aaatcgagtt aataaccggt tatcaattaa atgctttaga tggtaataat agtgaacttc aatcggttat agttaaagat ttacaaaata atactcgcaa attggatgcc aatatattac tacctttttt tggtttaaaa caagatttag gtagcttagc aaattggggt ttaaatgtta agcttcacca tattgaagta gactcttcct attatcaaac taatatagaa ggtatttacg ccattggcga tattgctcat tatgttggta agcttaagct gattcttacg ggttttgctg aagcggcaag cagtcttcat catgcttata gtagagtatt tgacggcaag gctctacatt ttgagtattc tactaccaaa aatacgggga aaagaagtaa gagcgtgact aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for RicoA.00893.a.B1.PW35900
PURIFICATION DATe: 6/22/2012
CONCENTRATION: 26.21mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 21
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMY NTDVVIIGAG PVGLFAVFQA GMLGMKCHVI DAQEVIGGQC ITLYPEKPIY DIPAYPKIAA EELIKQLALQ AAPFNPIYHL NQQAIELNKQ DDFFEIKTSK NTLIKSKVII IAAGAGSFGP NKPPLANIED FESKSVFYFI NDKSKFAGKN IVIAGGGDSA VDWAISLSDI ANKIYLVHRR DKFTAAPESV RQLRHIAETD KIELITGYQL NALDGNNSEL QSVIVKDLQN NTRKLDANIL LPFFGLKQDL GSLANWGLNV KLHHIEVDSS YYQTNIEGIY AIGDIAHYVG KLKLILTGFA EAASSLHHAY SRVFDGKALH FEYSTTKNTG KRSKS
Validated NT Sequence
cacgctctta cttcttttcc ccgtattttt ggtagtagaa tactcaaaat gtagagcctt gccgtcaaat actctactat aagcatgatg aagactgctt gccgcttcag caaaacccgt aagaatcagc ttaagcttac caacataatg agcaatatcg ccaatggcgt aaataccttc tatattagtt tgataatagg aagagtctac ttcaatatgg tgaagcttaa catttaaacc ccaatttgct aagctaccta aatcttgttt taaaccaaaa aaaggtagta atatattggc atccaatttg cgagtattat tttgtaaatc tttaactata accgattgaa gttcactatt attaccatct aaagcattta attgataacc ggttattaac tcgattttat cggtttctgc aatatgtctt aattgcctta cgctttcagg agctgccgta aatttatccc gcctatgtac taaatatatt ttatttgcaa tatctgaaag agaaatagcc cagtcaacag ccgaatcccc gccgcctgct ataactatat ttttacccgc aaatttgctt ttatcattga tgaaataaaa aactgacttg ctttcaaaat cttcaatatt cgcaaggggc ggtttgtttg gaccgaatga acctgctcct gctgcaataa taataacttt gcttttaata agagtgtttt ttgaggtttt aatttcaaaa aaatcatctt gtttatttag ctctatagct tgctgattta aatgatatat agggttaaaa ggagctgctt gaagtgctaa ctgttttatt agctcttctg ctgcaatttt aggatatgct ggtatatcgt aaataggctt ttcaggatat agggtaatac actgaccgcc gataacttct tgtgcatcaa taacatgaca cttcatgcca agcattcctg cttgaaaaac tgcaaataat ccaaccggac cggcacctat tattacaaca tcagtattat acatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMY NTDVVIIGAG PVGLFAVFQA GMLGMKCHVI DAQEVIGGQC ITLYPEKPIY DIPAYPKIAA EELIKQLALQ AAPFNPIYHL NQQAIELNKQ DDFFEIKTSK NTLIKSKVII IAAGAGSFGP NKPPLANIED FESKSVFYFI NDKSKFAGKN IVIAGGGDSA VDWAISLSDI ANKIYLVHRR DKFTAAPESV RQLRHIAETD KIELITGYQL NALDGNNSEL QSVIVKDLQN NTRKLDANIL LPFFGLKQDL GSLANWGLNV KLHHIEVDSS YYQTNIEGIY AIGDIAHYVG KLKLILTGFA EAASSLHHAY SRVFDGKALH FEYSTTKNTG KRSKSVTK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTATA ATACTGATGT TGTAATAATA GGTGCCGGTC CGGTTGGATT ATTTGCAGTT TTTCAAGCAG GAATGCTTGG CATGAAGTGT CATGTTATTG ATGCACAAGA AGTTATCGGC GGTCAGTGTA TTACCCTATA TCCTGAAAAG CCTATTTACG ATATACCAGC ATATCCTAAA ATTGCAGCAG AAGAGCTAAT AAAACAGTTA GCACTTCAAG CAGCTCCTTT TAACCCTATA TATCATTTAA ATCAGCAAGC TATAGAGCTA AATAAACAAG ATGATTTTTT TGAAATTAAA ACCTCAAAAA ACACTCTTAT TAAAAGCAAA GTTATTATTA TTGCAGCAGG AGCAGGTTCA TTCGGTCCAA ACAAACCGCC CCTTGCGAAT ATTGAAGATT TTGAAAGCAA GTCAGTTTTT TATTTCATCA ATGATAAAAG CAAATTTGCG GGTAAAAATA TAGTTATAGC AGGCGGCGGG GATTCGGCTG TTGACTGGGC TATTTCTCTT TCAGATATTG CAAATAAAAT ATATTTAGTA CATAGGCGGG ATAAATTTAC GGCAGCTCCT GAAAGCGTAA GGCAATTAAG ACATATTGCA GAAACCGATA AAATCGAGTT AATAACCGGT TATCAATTAA ATGCTTTAGA TGGTAATAAT AGTGAACTTC AATCGGTTAT AGTTAAAGAT TTACAAAATA ATACTCGCAA ATTGGATGCC AATATATTAC TACCTTTTTT TGGTTTAAAA CAAGATTTAG GTAGCTTAGC AAATTGGGGT TTAAATGTTA AGCTTCACCA TATTGAAGTA GACTCTTCCT ATTATCAAAC TAATATAGAA GGTATTTACG CCATTGGCGA TATTGCTCAT TATGTTGGTA AGCTTAAGCT GATTCTTACG GGTTTTGCTG AAGCGGCAAG CAGTCTTCAT CATGCTTATA GTAGAGTATT TGACGGCAAG GCTCTACATT TTGAGTATTC TACTACCAAA AATACGGGGA AAAGAAGTAA GAGCGTGACT AAATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT