RicoA.00339.a

Alanine racemase

CENTER ID: RicoA.00339.a
ORGANISM: Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE: Boutonneuse fever
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.00339.a.B1.GE35279 Full length( RicoA.00339.a ) 1 355
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.00339.a.B1.PW37271 Full length( RicoA.00339.a ) 1 355

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272944.4.peg.150
RefSeq: NP_359765.1
UniProt: Q92JD9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLCTVEINL STIKNNYLLL QDICKTSLVG AAVKANGYGL GAVQISKALI EENCRHFFVA SSEEGVNLRN ALGLDVNILV LNGVFEHDAL ELIEYNLTPV LNNLKQIEIW QKFSNLKNRL LPCYLHFNTG INRLGLSSDE IEQLINDRDL LKGLDLQYII SHLAISEEID NPYNLEQLNR FKAYLQYFPN VKASLANSGG IFLGQDYHFD LARPGAALYG LNPLTKNPVT LKAPIIHLQN LTLDSHIGYN MTFTTKRDSV IATLPLGYAD GFSRNFSNQG EVFINSRSVP IVGRVSMDLI NIDVTDLPPS EIFLGQEAEI IGNYCTPDKI ASIIGTIGYE VLTNLGSRYK RKYIG
NT Sequence
ATGAGCTTGT GTACTGTAGA AATAAATCTA TCTACAATAA AAAATAATTA CCTTTTATTA CAAGATATTT GCAAAACCTC ATTAGTCGGT GCTGCCGTTA AAGCTAACGG TTACGGTCTT GGAGCAGTAC AAATTTCTAA AGCTTTAATA GAAGAAAATT GTAGGCATTT TTTTGTGGCC TCAAGTGAAG AAGGAGTGAA TTTACGCAAT GCTTTAGGAT TAGATGTTAA TATTTTAGTT CTTAACGGCG TTTTTGAACA TGATGCTTTA GAGCTGATAG AATATAATTT AACACCTGTC CTAAATAACT TAAAACAAAT AGAAATTTGG CAAAAATTTA GTAATTTGAA AAACCGATTA TTGCCTTGTT ATTTACATTT CAATACGGGG ATTAATCGTC TTGGCTTAAG CTCTGATGAG ATAGAGCAGC TCATTAATGA TCGTGATTTG TTAAAAGGGC TAGATTTACA ATATATTATA AGCCATCTAG CTATATCCGA GGAAATAGAT AATCCTTATA ATCTAGAACA ATTAAACAGA TTTAAAGCTT ATTTACAATA TTTCCCAAAC GTCAAAGCAA GCCTTGCTAA TTCCGGCGGC ATATTTTTAG GACAGGATTA CCATTTTGAT TTAGCAAGAC CAGGGGCAGC CTTATATGGT CTTAATCCCT TAACAAAGAA TCCCGTAACC TTAAAAGCTC CTATAATTCA CTTACAGAAT TTGACACTAG ATAGCCATAT CGGTTATAAT ATGACTTTTA CAACTAAGCG TGATAGTGTC ATTGCAACGT TACCGCTTGG TTATGCCGAT GGGTTTAGTC GTAATTTTAG CAATCAAGGC GAAGTATTTA TTAACAGTCG CAGCGTTCCT ATAGTAGGGC GAGTATCGAT GGATTTAATA AATATCGATG TTACCGATCT ACCACCATCC GAGATTTTTC TAGGGCAGGA AGCGGAAATT ATCGGAAATT ATTGCACGCC CGATAAAATA GCGAGTATTA TAGGTACTAT CGGGTATGAG GTATTAACTA ACCTTGGTAG TAGATATAAA AGAAAGTATA TCGGG
Details for RicoA.00339.a.B1.GE35279
HARVESTED ON: 5/1/2012
SEQUENCED ON: 5/2/2012
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS LCTVEINLST IKNNYLLLQD ICKTSLVGAA VKAXGYGLGA VQISKALIEE NCRHFFVASS EEGVNLRNAL GLDVNILVLN GVFEHDALEL IEYNLTPVLN NLKQIEIWQK FSNLKNRLLP CYLHFNTGIN RLGLSSDEIE QLINDRDLLK GLDLQYIISH LAISEEIDNP YNLEQLNRFK AYLQYFPNVK ASLANSGGIF LGQDYHFDLA RPGAALYGLN PLTKNPVTLK APIIHLQNLT LDSHIGYNMT FTTKRDSVIA TLPLGYADGF SRNFSNQGEV FINSRSVPIV GRVSMDLINI DVTDLPPSEI FLGQEAEIIG NYCTPDKIAS IIGTIGYEVL TNLGSRYKRK YIG
Validated NT Sequence
tacccgatat actttctttt atatctacta ccaaggttag ttaatacctc atacccgata gtacctataa tactcgctat tttatcgggc gtgcaataat ttccgataat ttccgcttcc tgccctagaa aaatctcgga tggtggtaga tcggtaacat cgatatttat taaatccatc gatactcgcc ctactatagg aacgctgcga ctgttaataa atacttcgcc ttgattgcta aaattacgac taaacccatc ggcataacca agcggtaacg ttgcaatgac actatcacgc ttagttgtaa aagtcatatt ataaccgata tggctatcta gtgtcaaatt ctgtaagtga attataggag cttttaaggt tacgggattc tttgttaagg gattaagacc atataaggct gcccctggtc ttgctaaatc aaaatggtaa tcctgtccta aaaatatgcc gccggaatta gcaaggcttg ctttgacgtt tgggaaatat tgtaaataag ctttaaatct gtttaattgt tctagattat aaggattatc tatttcctcg gatatagcta gatggcttat aatatattgt aaatctagcc cttttaacaa atcacgatca ttaatgagct gctctatctc atcagagctt aagccaagac gattaatccc cgtattgaaa tgtaaataac aaggcaataa tcggtttttc aaattactaa atttttgcca aatttctatt tgttttaagt tatttaggac aggtgttaaa ttatattcta tcagctctaa agcatcatgt tcaaaaacgc cgttaagaac taaaatatta acatctaatc ctaaagcatt gcgtaaattc actccttctt cacttgaggc cacaaaaaaa tgcctacaat tttcttctat taaagcttta gaaatttgta ctgctccaag accgtaaccn ttagctttaa cggcagcacc gactaatgag gttttgcaaa tatcttgtaa taaaaggtaa ttatttttta ttgtagatag atttatttct acagtacaca agctcatatg gtggtggtgg tggtgagcca tggtat
Expected Protein Sequence
MAHHHHHHMS LCTVEINLST IKNNYLLLQD ICKTSLVGAA VKANGYGLGA VQISKALIEE NCRHFFVASS EEGVNLRNAL GLDVNILVLN GVFEHDALEL IEYNLTPVLN NLKQIEIWQK FSNLKNRLLP CYLHFNTGIN RLGLSSDEIE QLINDRDLLK GLDLQYIISH LAISEEIDNP YNLEQLNRFK AYLQYFPNVK ASLANSGGIF LGQDYHFDLA RPGAALYGLN PLTKNPVTLK APIIHLQNLT LDSHIGYNMT FTTKRDSVIA TLPLGYADGF SRNFSNQGEV FINSRSVPIV GRVSMDLINI DVTDLPPSEI FLGQEAEIIG NYCTPDKIAS IIGTIGYEVL TNLGSRYKRK YIG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagct tgtgtactgt agaaataaat ctatctacaa taaaaaataa ttacctttta ttacaagata tttgcaaaac ctcattagtc ggtgctgccg ttaaagctaa cggttacggt cttggagcag tacaaatttc taaagcttta atagaagaaa attgtaggca tttttttgtg gcctcaagtg aagaaggagt gaatttacgc aatgctttag gattagatgt taatatttta gttcttaacg gcgtttttga acatgatgct ttagagctga tagaatataa tttaacacct gtcctaaata acttaaaaca aatagaaatt tggcaaaaat ttagtaattt gaaaaaccga ttattgcctt gttatttaca tttcaatacg gggattaatc gtcttggctt aagctctgat gagatagagc agctcattaa tgatcgtgat ttgttaaaag ggctagattt acaatatatt ataagccatc tagctatatc cgaggaaata gataatcctt ataatctaga acaattaaac agatttaaag cttatttaca atatttccca aacgtcaaag caagccttgc taattccggc ggcatatttt taggacagga ttaccatttt gatttagcaa gaccaggggc agccttatat ggtcttaatc ccttaacaaa gaatcccgta accttaaaag ctcctataat tcacttacag aatttgacac tagatagcca tatcggttat aatatgactt ttacaactaa gcgtgatagt gtcattgcaa cgttaccgct tggttatgcc gatgggttta gtcgtaattt tagcaatcaa ggcgaagtat ttattaacag tcgcagcgtt cctatagtag ggcgagtatc gatggattta ataaatatcg atgttaccga tctaccacca tccgagattt ttctagggca ggaagcggaa attatcggaa attattgcac gcccgataaa atagcgagta ttataggtac tatcgggtat gaggtattaa ctaaccttgg tagtagatat aaaagaaagt atatcgggtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for RicoA.00339.a.B1.PW37271
PURIFICATION DATe: 4/1/2013
CONCENTRATION: 1.36mg/ml
OBSERVED MW: 37kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS LCTVEINLST IKNNYLLLQD ICKTSLVGAA VKAXGYGLGA VQISKALIEE NCRHFFVASS EEGVNLRNAL GLDVNILVLN GVFEHDALEL IEYNLTPVLN NLKQIEIWQK FSNLKNRLLP CYLHFNTGIN RLGLSSDEIE QLINDRDLLK GLDLQYIISH LAISEEIDNP YNLEQLNRFK AYLQYFPNVK ASLANSGGIF LGQDYHFDLA RPGAALYGLN PLTKNPVTLK APIIHLQNLT LDSHIGYNMT FTTKRDSVIA TLPLGYADGF SRNFSNQGEV FINSRSVPIV GRVSMDLINI DVTDLPPSEI FLGQEAEIIG NYCTPDKIAS IIGTIGYEVL TNLGSRYKRK YIG
Validated NT Sequence
tacccgatat actttctttt atatctacta ccaaggttag ttaatacctc atacccgata gtacctataa tactcgctat tttatcgggc gtgcaataat ttccgataat ttccgcttcc tgccctagaa aaatctcgga tggtggtaga tcggtaacat cgatatttat taaatccatc gatactcgcc ctactatagg aacgctgcga ctgttaataa atacttcgcc ttgattgcta aaattacgac taaacccatc ggcataacca agcggtaacg ttgcaatgac actatcacgc ttagttgtaa aagtcatatt ataaccgata tggctatcta gtgtcaaatt ctgtaagtga attataggag cttttaaggt tacgggattc tttgttaagg gattaagacc atataaggct gcccctggtc ttgctaaatc aaaatggtaa tcctgtccta aaaatatgcc gccggaatta gcaaggcttg ctttgacgtt tgggaaatat tgtaaataag ctttaaatct gtttaattgt tctagattat aaggattatc tatttcctcg gatatagcta gatggcttat aatatattgt aaatctagcc cttttaacaa atcacgatca ttaatgagct gctctatctc atcagagctt aagccaagac gattaatccc cgtattgaaa tgtaaataac aaggcaataa tcggtttttc aaattactaa atttttgcca aatttctatt tgttttaagt tatttaggac aggtgttaaa ttatattcta tcagctctaa agcatcatgt tcaaaaacgc cgttaagaac taaaatatta acatctaatc ctaaagcatt gcgtaaattc actccttctt cacttgaggc cacaaaaaaa tgcctacaat tttcttctat taaagcttta gaaatttgta ctgctccaag accgtaaccn ttagctttaa cggcagcacc gactaatgag gttttgcaaa tatcttgtaa taaaaggtaa ttatttttta ttgtagatag atttatttct acagtacaca agctcatatg gtggtggtgg tggtgagcca tggtat
Expressed Protein Sequence
MAHHHHHHMS LCTVEINLST IKNNYLLLQD ICKTSLVGAA VKANGYGLGA VQISKALIEE NCRHFFVASS EEGVNLRNAL GLDVNILVLN GVFEHDALEL IEYNLTPVLN NLKQIEIWQK FSNLKNRLLP CYLHFNTGIN RLGLSSDEIE QLINDRDLLK GLDLQYIISH LAISEEIDNP YNLEQLNRFK AYLQYFPNVK ASLANSGGIF LGQDYHFDLA RPGAALYGLN PLTKNPVTLK APIIHLQNLT LDSHIGYNMT FTTKRDSVIA TLPLGYADGF SRNFSNQGEV FINSRSVPIV GRVSMDLINI DVTDLPPSEI FLGQEAEIIG NYCTPDKIAS IIGTIGYEVL TNLGSRYKRK YIG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCT TGTGTACTGT AGAAATAAAT CTATCTACAA TAAAAAATAA TTACCTTTTA TTACAAGATA TTTGCAAAAC CTCATTAGTC GGTGCTGCCG TTAAAGCTAA CGGTTACGGT CTTGGAGCAG TACAAATTTC TAAAGCTTTA ATAGAAGAAA ATTGTAGGCA TTTTTTTGTG GCCTCAAGTG AAGAAGGAGT GAATTTACGC AATGCTTTAG GATTAGATGT TAATATTTTA GTTCTTAACG GCGTTTTTGA ACATGATGCT TTAGAGCTGA TAGAATATAA TTTAACACCT GTCCTAAATA ACTTAAAACA AATAGAAATT TGGCAAAAAT TTAGTAATTT GAAAAACCGA TTATTGCCTT GTTATTTACA TTTCAATACG GGGATTAATC GTCTTGGCTT AAGCTCTGAT GAGATAGAGC AGCTCATTAA TGATCGTGAT TTGTTAAAAG GGCTAGATTT ACAATATATT ATAAGCCATC TAGCTATATC CGAGGAAATA GATAATCCTT ATAATCTAGA ACAATTAAAC AGATTTAAAG CTTATTTACA ATATTTCCCA AACGTCAAAG CAAGCCTTGC TAATTCCGGC GGCATATTTT TAGGACAGGA TTACCATTTT GATTTAGCAA GACCAGGGGC AGCCTTATAT GGTCTTAATC CCTTAACAAA GAATCCCGTA ACCTTAAAAG CTCCTATAAT TCACTTACAG AATTTGACAC TAGATAGCCA TATCGGTTAT AATATGACTT TTACAACTAA GCGTGATAGT GTCATTGCAA CGTTACCGCT TGGTTATGCC GATGGGTTTA GTCGTAATTT TAGCAATCAA GGCGAAGTAT TTATTAACAG TCGCAGCGTT CCTATAGTAG GGCGAGTATC GATGGATTTA ATAAATATCG ATGTTACCGA TCTACCACCA TCCGAGATTT TTCTAGGGCA GGAAGCGGAA ATTATCGGAA ATTATTGCAC GCCCGATAAA ATAGCGAGTA TTATAGGTAC TATCGGGTAT GAGGTATTAA CTAACCTTGG TAGTAGATAT AAAAGAAAGT ATATCGGGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT