RicoA.00159.a

ATP-dependent RNA helicase RhlE

CENTER ID: RicoA.00159.a
ORGANISM: Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE: Boutonneuse fever
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.00159.a.B1.GE35277 Full length( RicoA.00159.a ) 1 414
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272944.4.peg.1161
RefSeq: NP_360657.1
UniProt: Q92GV2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDYIMKNFNL SEELIIALET MNITEPTEIQ KQSIPVAMAG SDILASSQTG SGKTLAYLLP LIDSFIKNKT TALILVPTRE LATQIHSTLN KVTTSYKINS AVLIGGEPMP KQFIQLKKNP KVIIGTPGRI IDHLNRGSLK IDRIGITVLD EMDRMLDMGM KEQLEEINKF LPEKRQVLMF SATMPKHIIA VSQKYLNNPV RITVGATNKA AAEIKQESMH VSDKEKFSAL TKQLGNREGS VIIFVKTKRS ADQLAKMLKY ENHTAEAIHG DLSQRQRERV ILSFRKSNHR IMVATDVAAR GLDIPHTQHV INYDLPMCPE DYLHRIGRTG RAGATGHALS FISPDDVIRW RAIDRLVNKG ESTPRSEFRS DKNNRKRSFG KRAGGDGKKF NAFDSSRKRT FDGNSYGNKR KKVS
NT Sequence
TTGGATTATA TAATGAAAAA TTTTAATTTA TCTGAAGAAT TAATTATTGC TCTCGAGACA ATGAATATAA CTGAGCCGAC TGAAATACAA AAGCAATCGA TTCCGGTTGC AATGGCGGGA TCGGACATAC TCGCTTCTAG CCAAACAGGT TCAGGCAAAA CTCTTGCTTA TTTATTGCCG TTAATTGATT CATTTATTAA AAATAAAACT ACTGCTTTAA TTCTTGTTCC GACTAGAGAA TTAGCAACGC AAATACACAG TACTTTAAAT AAAGTAACTA CATCGTATAA AATTAATAGT GCAGTTTTGA TAGGCGGTGA ACCGATGCCT AAACAATTTA TACAATTAAA AAAGAATCCA AAAGTGATTA TCGGTACGCC GGGGCGTATT ATCGATCACT TAAATAGGGG AAGTCTAAAA ATTGATCGTA TAGGCATAAC CGTACTTGAC GAAATGGATA GAATGCTTGA TATGGGGATG AAAGAACAAT TAGAAGAAAT CAATAAATTT TTACCGGAAA AAAGACAAGT TTTAATGTTT TCCGCTACTA TGCCAAAACA TATTATTGCT GTTTCTCAAA AATATCTAAA CAATCCGGTA CGTATTACGG TAGGTGCTAC TAATAAAGCA GCTGCAGAAA TAAAACAGGA ATCGATGCAT GTTTCTGATA AAGAAAAATT TAGTGCATTA ACTAAACAAC TTGGTAATAG GGAAGGATCG GTCATTATTT TTGTGAAGAC TAAACGCTCT GCCGATCAAT TAGCTAAAAT GTTGAAATAT GAAAATCATA CAGCAGAAGC TATACACGGT GATTTAAGTC AGCGTCAACG TGAAAGAGTA ATTTTATCAT TTCGTAAGTC AAACCATAGA ATAATGGTAG CAACTGATGT AGCGGCTCGT GGGCTTGATA TTCCTCATAC ACAGCATGTT ATTAATTATG ATTTACCTAT GTGTCCTGAA GATTATTTAC ATAGGATAGG TAGAACGGGT AGAGCAGGGG CTACCGGACA TGCGTTGTCT TTTATTTCTC CTGATGATGT TATTAGATGG CGTGCAATCG ATCGTTTAGT AAATAAAGGA GAATCTACGC CACGTAGTGA GTTTAGAAGT GATAAAAATA ATCGTAAAAG ATCATTCGGT AAAAGAGCCG GCGGTGACGG TAAAAAGTTT AATGCTTTTG ATAGTAGCCG TAAAAGAACC TTTGATGGTA ACAGCTACGG TAATAAAAGA AAGAAAGTAT CG
Details for RicoA.00159.a.B1.GE35277
HARVESTED ON: 5/1/2012
SEQUENCED ON: 5/2/2012
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MKNFNLSEEL IIALETMNIT EPTEIQKQSI PVAMAGSDIL ASSQTGSGKT LAYLLPLIDS FIKNKTTALI LVPTRELATQ IHSTLNKVTT SYKINSAVLI GGEPMPKQFI QLKKNPKVII GTPGRIIDHL NRGSLKIDRI GITVLDEMDR MLDMGMKEQL EEINKFLPEK RQVLMFSATM PKHIIAVSQK YLNNPVRITV GATNKAAAEI KQESMHVSDK EKFSALTKQL GNREGSVIIF VKTKRSADQL AKMLKYENHT AEAIHGDLSQ RQRERVILSF RKSNHRIMVA TDVAARGLDI PHTQHVINYD LPMCPEDYLH RIGRTGRAGA TGHALSFISP DDVIRWRAID RLVNKGESTP RSEFRSDKNN RKRSFGKRAG GDGKKFNAFD SSRKRTFDGN SYGNKRKKVS
Validated NT Sequence
atcttactca cttacgatac tttctttctt ttattaccgt agctgttacc atcaaaggtt cttttacggc tactatcaaa agcattaaac tttttaccgt caccgccggc tcttttaccg aatgatcttt tacgattatt tttatcactt ctaaactcac tacgtggcgt agattctcct ttatttacta aacgatcgat tgcacgccat ctaataacat catcaggaga aataaaagac aacgcatgtc cggtagcccc tgctctaccc gttctaccta tcctatgtaa ataatcttca ggacacatag gtaaatcata attaataaca tgctgtgtat gaggaatatc aagcccacga gccgctacat cagttgctac cattattcta tggtttgact tacgaaatga taaaattact ctttcacgtt gacgctgact taaatcaccg tgtatagctt ctgctgtatg attttcatat ttcaacattt tagctaattg atcggcagag cgtttagtct tcacaaaaat aatgaccgat ccttccctat taccaagttg tttagttaat gcactaaatt tttctttatc agaaacatgc atcgattcct gttttatttc tgcagctgct ttattagtag cacctaccgt aatacgtacc ggattgttta gatatttttg agaaacagca ataatatgtt ttggcatagt agcggaaaac attaaaactt gtcttttttc cggtaaaaat ttattgattt cttctaattg ttctttcatc cccatatcaa gcattctatc catttcgtca agtacggtta tgcctatacg atcaattttt agacttcccc tatttaagtg atcgataata cgccccggcg taccgataat cacttttgga ttctttttta attgtataaa ttgtttaggc atcggttcac cgcctatcaa aactgcacta ttaattttat acgatgtagt tactttattt aaagtactgt gtatttgcgt tgctaattct ctagtcggaa caagaattaa agcagtagtt ttatttttaa taaatgaatc aattaacggc aataaataag caagagtttt gcctgaacct gtttggctag aagcgagtat gtccgatccc gccattgcaa ccggaatcga ttgcttttgt atttcagtcg gctcagttat attcattgtc tcgagagcaa taattaattc ttcagataaa ttaaaatttt tcattatata a
Expected Protein Sequence
MAHHHHHHML DYIMKNFNLS EELIIALETM NITEPTEIQK QSIPVAMAGS DILASSQTGS GKTLAYLLPL IDSFIKNKTT ALILVPTREL ATQIHSTLNK VTTSYKINSA VLIGGEPMPK QFIQLKKNPK VIIGTPGRII DHLNRGSLKI DRIGITVLDE MDRMLDMGMK EQLEEINKFL PEKRQVLMFS ATMPKHIIAV SQKYLNNPVR ITVGATNKAA AEIKQESMHV SDKEKFSALT KQLGNREGSV IIFVKTKRSA DQLAKMLKYE NHTAEAIHGD LSQRQRERVI LSFRKSNHRI MVATDVAARG LDIPHTQHVI NYDLPMCPED YLHRIGRTGR AGATGHALSF ISPDDVIRWR AIDRLVNKGE STPRSEFRSD KNNRKRSFGK RAGGDGKKFN AFDSSRKRTF DGNSYGNKRK KVS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgg attatataat gaaaaatttt aatttatctg aagaattaat tattgctctc gagacaatga atataactga gccgactgaa atacaaaagc aatcgattcc ggttgcaatg gcgggatcgg acatactcgc ttctagccaa acaggttcag gcaaaactct tgcttattta ttgccgttaa ttgattcatt tattaaaaat aaaactactg ctttaattct tgttccgact agagaattag caacgcaaat acacagtact ttaaataaag taactacatc gtataaaatt aatagtgcag ttttgatagg cggtgaaccg atgcctaaac aatttataca attaaaaaag aatccaaaag tgattatcgg tacgccgggg cgtattatcg atcacttaaa taggggaagt ctaaaaattg atcgtatagg cataaccgta cttgacgaaa tggatagaat gcttgatatg gggatgaaag aacaattaga agaaatcaat aaatttttac cggaaaaaag acaagtttta atgttttccg ctactatgcc aaaacatatt attgctgttt ctcaaaaata tctaaacaat ccggtacgta ttacggtagg tgctactaat aaagcagctg cagaaataaa acaggaatcg atgcatgttt ctgataaaga aaaatttagt gcattaacta aacaacttgg taatagggaa ggatcggtca ttatttttgt gaagactaaa cgctctgccg atcaattagc taaaatgttg aaatatgaaa atcatacagc agaagctata cacggtgatt taagtcagcg tcaacgtgaa agagtaattt tatcatttcg taagtcaaac catagaataa tggtagcaac tgatgtagcg gctcgtgggc ttgatattcc tcatacacag catgttatta attatgattt acctatgtgt cctgaagatt atttacatag gataggtaga acgggtagag caggggctac cggacatgcg ttgtctttta tttctcctga tgatgttatt agatggcgtg caatcgatcg tttagtaaat aaaggagaat ctacgccacg tagtgagttt agaagtgata aaaataatcg taaaagatca ttcggtaaaa gagccggcgg tgacggtaaa aagtttaatg cttttgatag tagccgtaaa agaacctttg atggtaacag ctacggtaat aaaagaaaga aagtatcgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat