SSGCID: Target Page

RicoA.00157.a

DNA topoisomerase 4 subunit A

CENTER ID:	RicoA.00157.a
ORGANISM:	Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE:	Boutonneuse fever
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RicoA.00157.a.B1.GE35276	Full length( RicoA.00157.a )		1	738

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272944.4.peg.115
RefSeq:	NP_359734.1
UniProt:	Q92JH0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKEAKVENID FGNALSERYL AYALSTIMSR SLPDVRDGLK PVHRRLLYAM LQLRLEPNSG YKKCARVVGD VIGKYHPHGD VAVYDTLVRL AQHFSLRYPL IDGQGNFGSI DGDNAAAMRY TESRMTDICT LLMEDIDKDT VDFRPTYDGS DLEPVIMPAS FPNLLANGSE GIAVGMATNI PPHNLHELCD ALIHLINHPK AEINDIINFV KGPDFPTGGI IIDKAEVINA AYTTGRGSLR VRSRWEKEEL SYGTYQIVVT EIPYQVQKSK LIEQIAILLK DKKIPLVSNI RDESTDIIRL VIEPRDRGCD PQIVMESLFK LTNLESRIQL NMNVIGSNNV PRVMNILEIL QEFLYHRQNI VTRRSTYLLN KIKHRLEILK GLRIAYLNLD EIIKIIREED EPKAIMMEWF KLTEIQVEAI LNTRLRSLRK LEEQEIINEH SNLQKQQAIL EEILNNPKEL WKIVKKEIKT VQTKFGLNTV IGARRTSFEE VTLTNQVVDI TAFITKEPIT IICSKMGWVR SLKGHNTDLS TIKYKEGDAE KFILEAYTTD KILIVSSEGR FFTLLADNIS KGKGTGESIK LLVDIGNNDI TNILVYKPDQ LLLLASSVGK GFLVNSNEVM AQTKTGKQIM NVPDGHVCIA CLPVNGDSIA CIGESRKLLV FNIDEIPEMK KGQGVTLQKF KNAKLLDIKI FNKEDGLGWN NNGKVKLEKN IVAFLGKRGS TGKLPPMGFP KNNRFSSY

NT Sequence

ATGAAAGAAG CTAAAGTAGA AAATATTGAC TTTGGTAATG CATTATCAGA GCGTTATCTT GCTTATGCGT TATCAACAAT TATGTCACGT TCGCTTCCTG ACGTACGTGA CGGACTCAAG CCTGTACATC GTAGGTTATT ATACGCAATG CTACAGTTAA GGCTTGAGCC GAATTCCGGC TATAAGAAAT GTGCGAGGGT GGTCGGTGAC GTAATAGGTA AATACCACCC GCACGGTGAT GTGGCAGTGT ATGATACCTT GGTACGCCTT GCTCAGCATT TTTCGTTGCG TTATCCTTTA ATTGATGGGC AGGGTAATTT CGGTTCTATC GACGGTGATA ATGCAGCAGC TATGCGTTAT ACCGAATCAC GTATGACGGA CATATGTACG TTATTAATGG AGGACATCGA TAAAGATACG GTAGATTTTC GTCCTACTTA TGATGGTTCC GATTTAGAAC CGGTAATAAT GCCGGCAAGT TTTCCGAATT TACTAGCTAA TGGTTCTGAA GGGATAGCAG TCGGTATGGC AACCAATATA CCGCCGCATA ATTTACATGA GCTTTGTGAT GCTCTAATAC ATTTAATCAA CCATCCGAAA GCTGAAATTA ACGATATAAT AAACTTTGTC AAAGGTCCTG ATTTCCCGAC CGGCGGCATA ATTATTGATA AAGCTGAAGT TATTAATGCT GCATATACTA CTGGACGCGG TAGTCTTAGA GTAAGAAGCA GATGGGAAAA AGAGGAGCTA AGTTACGGTA CATATCAGAT AGTCGTAACC GAGATACCTT ATCAGGTTCA GAAATCAAAA CTTATAGAAC AAATAGCAAT ATTACTAAAG GATAAAAAAA TTCCCCTTGT TAGCAATATT AGAGATGAAT CAACAGACAT TATAAGGTTG GTTATAGAGC CGAGAGATCG GGGATGTGAT CCGCAAATAG TTATGGAATC TCTATTCAAG CTAACGAATT TAGAAAGCCG CATTCAGTTA AATATGAATG TGATCGGCAG TAATAACGTG CCGAGAGTAA TGAATATTTT AGAGATTTTA CAAGAGTTTC TATATCATAG ACAAAATATT GTTACTCGCA GATCAACTTA TCTTTTAAAC AAAATCAAGC ATCGTTTAGA AATTCTAAAA GGGCTTAGGA TAGCTTATCT AAATCTTGAT GAGATCATTA AAATTATTCG TGAAGAAGAT GAGCCAAAAG CGATAATGAT GGAGTGGTTT AAACTAACCG AAATACAAGT AGAAGCGATA TTAAATACCC GTCTTCGCTC GCTTCGTAAG CTTGAAGAGC AGGAAATTAT TAATGAGCAT AGTAATTTGC AGAAGCAACA AGCTATATTA GAAGAAATTC TAAATAATCC TAAAGAACTA TGGAAAATAG TTAAAAAAGA AATCAAAACA GTACAAACGA AATTTGGATT AAATACGGTA ATAGGAGCAA GGCGTACAAG CTTTGAGGAA GTAACGCTTA CGAATCAAGT AGTTGATATA ACGGCTTTCA TTACTAAAGA GCCGATTACT ATTATCTGCT CAAAAATGGG GTGGGTGCGT TCGTTAAAAG GTCATAACAC CGATTTATCG ACTATCAAAT ATAAAGAAGG TGATGCAGAA AAATTTATTT TAGAAGCTTA CACGACTGAT AAAATATTAA TAGTTAGTTC AGAAGGCAGA TTTTTTACTT TGCTTGCCGA TAATATTTCC AAAGGTAAAG GAACAGGGGA ATCTATAAAA CTACTTGTGG ATATAGGTAA TAACGATATT ACGAATATTT TAGTCTATAA ACCTGATCAG CTTTTATTAC TTGCAAGTAG TGTAGGTAAG GGGTTTTTAG TTAATTCAAA TGAAGTAATG GCTCAAACAA AAACAGGAAA GCAAATTATG AATGTACCGG ATGGTCATGT ATGTATAGCT TGTTTACCTG TAAACGGTGA TAGTATTGCT TGTATTGGTG AAAGTCGTAA ATTACTGGTA TTTAATATAG ATGAAATACC TGAAATGAAA AAAGGGCAAG GTGTAACGCT GCAAAAATTT AAGAATGCTA AGCTTCTAGA TATTAAAATA TTCAATAAAG AAGACGGCTT AGGTTGGAAT AACAATGGAA AAGTAAAACT TGAGAAAAAC ATTGTTGCAT TTTTAGGCAA AAGAGGTAGT ACCGGTAAAT TACCTCCTAT GGGCTTCCCA AAAAATAATA GATTTTCATC ATAT

Details for RicoA.00157.a.B1.GE35276

HARVESTED ON:	5/1/2012
SEQUENCED ON:	5/2/2012
EXPECTED MW:	84kDa
OBSERVED MW:	84kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	41

Validated AA Sequence

Validated NT Sequence

ttnnttaact ttaagaagga ganataccat ggctcaccac caccaccacc atatgaaaga agctaaagta gaaaatattg actttggtaa tgcattatca gagcgttatc ttgcttatgc gttatcaaca attatgtcac gttcgcttcc tgacgtacgt gacggactca agcctgtaca tcgtaggtta ttatacgcaa tgctacagtt aaggcttgag ccgaattccg gctataagaa atgtgcgagg gtggtcggtg acgtaatagg taaataccac ccgcacggtg atgtggcagt gtatgatacc ttggtacgcc ttgctcagca tttttcgttg cgttatcctt taattgatgg gcagggtaat ttcggttcta tcgacggtga taatgcagca gctatgcgtt ataccgaatc acgtatgacg gacatatgta cgttattaat ggaggacatc gataaagata cggtagattt tcgtcctact tatgatggtt ccgatttaga accggtaata atgccggcaa gttttccgaa tttactagct aatggttctg aagggatagc agtcggtatg gcaaccaata taccgccgca taatttacat gagctttgtg atgctctaat acatttaatc aaccatccga aagctgaaat taacgatata ataaactttg tcaaaggtcc tgatttcccg accggcggca taattattga taaagctgaa gttattaatg ctgcatatac tactggacgc ggtagtctta gagtaagaag cagatgggaa aaagaggagc taagttacgg tacatatcag atagtcgtaa ccgagatacc ttatcaggtt cagaaatcaa aacttataga acaaatagca atattactaa aggataaaaa attccccttg ntagcaatat tagagatgaa tcnacngaca ttatanngnt ggntatagag ccnanagatc ggggnngtga tccgcaaata gttatggaat ctctantcaa gctaacgaat ttanaaagcn gcatnnngtn nnnnngaatg ngatcngnag tantnacnnn ncnnnngnnn nannantttn nnnnntttnn nannnnnttc nnnnnnnnnn nnnnnaannn tnnncnnncn nannnnnnnn nnnnntnnan nnnanncatt nnnaannnnn annnntnnnn nnntnnnnnn nnnnnaannn nnnnnnnnnn nnnnnnnnnn ngnnna

Expected Protein Sequence

MAHHHHHHMK EAKVENIDFG NALSERYLAY ALSTIMSRSL PDVRDGLKPV HRRLLYAMLQ LRLEPNSGYK KCARVVGDVI GKYHPHGDVA VYDTLVRLAQ HFSLRYPLID GQGNFGSIDG DNAAAMRYTE SRMTDICTLL MEDIDKDTVD FRPTYDGSDL EPVIMPASFP NLLANGSEGI AVGMATNIPP HNLHELCDAL IHLINHPKAE INDIINFVKG PDFPTGGIII DKAEVINAAY TTGRGSLRVR SRWEKEELSY GTYQIVVTEI PYQVQKSKLI EQIAILLKDK KIPLVSNIRD ESTDIIRLVI EPRDRGCDPQ IVMESLFKLT NLESRIQLNM NVIGSNNVPR VMNILEILQE FLYHRQNIVT RRSTYLLNKI KHRLEILKGL RIAYLNLDEI IKIIREEDEP KAIMMEWFKL TEIQVEAILN TRLRSLRKLE EQEIINEHSN LQKQQAILEE ILNNPKELWK IVKKEIKTVQ TKFGLNTVIG ARRTSFEEVT LTNQVVDITA FITKEPITII CSKMGWVRSL KGHNTDLSTI KYKEGDAEKF ILEAYTTDKI LIVSSEGRFF TLLADNISKG KGTGESIKLL VDIGNNDITN ILVYKPDQLL LLASSVGKGF LVNSNEVMAQ TKTGKQIMNV PDGHVCIACL PVNGDSIACI GESRKLLVFN IDEIPEMKKG QGVTLQKFKN AKLLDIKIFN KEDGLGWNNN GKVKLEKNIV AFLGKRGSTG KLPPMGFPKN NRFSSY

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag aagctaaagt agaaaatatt gactttggta atgcattatc agagcgttat cttgcttatg cgttatcaac aattatgtca cgttcgcttc ctgacgtacg tgacggactc aagcctgtac atcgtaggtt attatacgca atgctacagt taaggcttga gccgaattcc ggctataaga aatgtgcgag ggtggtcggt gacgtaatag gtaaatacca cccgcacggt gatgtggcag tgtatgatac cttggtacgc cttgctcagc atttttcgtt gcgttatcct ttaattgatg ggcagggtaa tttcggttct atcgacggtg ataatgcagc agctatgcgt tataccgaat cacgtatgac ggacatatgt acgttattaa tggaggacat cgataaagat acggtagatt ttcgtcctac ttatgatggt tccgatttag aaccggtaat aatgccggca agttttccga atttactagc taatggttct gaagggatag cagtcggtat ggcaaccaat ataccgccgc ataatttaca tgagctttgt gatgctctaa tacatttaat caaccatccg aaagctgaaa ttaacgatat aataaacttt gtcaaaggtc ctgatttccc gaccggcggc ataattattg ataaagctga agttattaat gctgcatata ctactggacg cggtagtctt agagtaagaa gcagatggga aaaagaggag ctaagttacg gtacatatca gatagtcgta accgagatac cttatcaggt tcagaaatca aaacttatag aacaaatagc aatattacta aaggataaaa aaattcccct tgttagcaat attagagatg aatcaacaga cattataagg ttggttatag agccgagaga tcggggatgt gatccgcaaa tagttatgga atctctattc aagctaacga atttagaaag ccgcattcag ttaaatatga atgtgatcgg cagtaataac gtgccgagag taatgaatat tttagagatt ttacaagagt ttctatatca tagacaaaat attgttactc gcagatcaac ttatctttta aacaaaatca agcatcgttt agaaattcta aaagggctta ggatagctta tctaaatctt gatgagatca ttaaaattat tcgtgaagaa gatgagccaa aagcgataat gatggagtgg tttaaactaa ccgaaataca agtagaagcg atattaaata cccgtcttcg ctcgcttcgt aagcttgaag agcaggaaat tattaatgag catagtaatt tgcagaagca acaagctata ttagaagaaa ttctaaataa tcctaaagaa ctatggaaaa tagttaaaaa agaaatcaaa acagtacaaa cgaaatttgg attaaatacg gtaataggag caaggcgtac aagctttgag gaagtaacgc ttacgaatca agtagttgat ataacggctt tcattactaa agagccgatt actattatct gctcaaaaat ggggtgggtg cgttcgttaa aaggtcataa caccgattta tcgactatca aatataaaga aggtgatgca gaaaaattta ttttagaagc ttacacgact gataaaatat taatagttag ttcagaaggc agatttttta ctttgcttgc cgataatatt tccaaaggta aaggaacagg ggaatctata aaactacttg tggatatagg taataacgat attacgaata ttttagtcta taaacctgat cagcttttat tacttgcaag tagtgtaggt aaggggtttt tagttaattc aaatgaagta atggctcaaa caaaaacagg aaagcaaatt atgaatgtac cggatggtca tgtatgtata gcttgtttac ctgtaaacgg tgatagtatt gcttgtattg gtgaaagtcg taaattactg gtatttaata tagatgaaat acctgaaatg aaaaaagggc aaggtgtaac gctgcaaaaa tttaagaatg ctaagcttct agatattaaa atattcaata aagaagacgg cttaggttgg aataacaatg gaaaagtaaa acttgagaaa aacattgttg catttttagg caaaagaggt agtaccggta aattacctcc tatgggcttc ccaaaaaata atagattttc atcatattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at