SSGCID: Target Page

RicoA.00148.a

Tyrosine recombinase xerC

CENTER ID:	RicoA.00148.a
ORGANISM:	Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE:	Boutonneuse fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RicoA.00148.a.B1.GE35274	Full length( RicoA.00148.a )		1	305

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272944.4.peg.1455
RefSeq:	NP_360905.1
UniProt:	Q92G55

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLDTSIQALI NKWQKYLALQ RNYSNHTVIS YNNDLKHFLE FMNYYNSELV TINHIKTADI RLIRSWLAKR NCDNFTASSI SRGLSAVKNF YRFLEKTTQL NSHIIFSIKS PKKTKLLPKA LSEDDVVISL EHIEEYGNVK WVELRNKALL VLIYASGLRI SEALSITKLH LQNLEFIRII GKGSKERIIP WLPIAKNLIT QYLEILPYKL GDNEPIFRGK QGKKLQPPVF NRELIKLKHF YGLPQHLTAH SFRHSFASHL LEHGADLRSL QELLGHKSLS TTQNYTKTSI KHLEAVYTTA YPIKK

NT Sequence

ATGTTAGATA CATCAATTCA AGCATTAATA AATAAGTGGC AAAAATACCT CGCTTTGCAA AGAAATTACT CTAATCATAC AGTAATTTCT TATAATAATG ACCTTAAGCA TTTTCTTGAG TTTATGAATT ATTATAATTC AGAGCTTGTA ACGATAAATC ATATTAAAAC CGCAGATATC AGATTAATCC GAAGTTGGCT TGCAAAACGT AACTGTGATA ATTTTACCGC TTCTTCAATT TCACGTGGTT TATCTGCAGT AAAAAATTTT TATAGGTTTT TAGAGAAAAC AACACAATTA AATAGTCATA TAATTTTTTC TATAAAATCT CCTAAAAAGA CTAAATTGTT ACCAAAAGCT TTATCGGAAG ATGATGTAGT AATATCACTT GAACATATTG AAGAATACGG GAATGTTAAG TGGGTAGAAC TTAGAAATAA AGCCCTACTT GTCCTTATAT ATGCTTCAGG TCTACGTATA TCGGAAGCAT TATCAATTAC GAAACTTCAT CTACAAAATC TAGAATTTAT AAGAATAATA GGTAAAGGTA GTAAAGAAAG AATAATTCCG TGGTTACCGA TTGCTAAAAA TTTAATTACC CAATATTTAG AAATATTGCC GTATAAGTTA GGTGATAATG AACCCATATT TAGAGGAAAG CAGGGTAAGA AATTGCAACC ACCGGTATTT AATCGTGAAT TAATTAAATT AAAGCATTTT TATGGTTTAC CGCAGCATTT AACTGCTCAT TCATTTAGAC ATAGTTTTGC TTCACATTTG CTTGAGCATG GTGCAGATTT ACGCTCTCTT CAAGAACTAT TAGGTCATAA AAGTCTATCT ACTACACAAA ACTATACTAA AACAAGTATA AAGCATTTAG AAGCTGTATA TACTACCGCA TATCCAATTA AAAAA

Details for RicoA.00148.a.B1.GE35274

HARVESTED ON:	5/1/2012
SEQUENCED ON:	5/2/2012
EXPECTED MW:	36kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	101

Validated AA Sequence

MAHHHHHHML DTSIQALINK WQKYLALQRN YSNHTVISYN NDLKHFLEFM NYYNSELVTI NHIKTADIRL IRSWLAKRNC DNFTASSISR GLSAVKNFYR FLEKTTQLNS HIIFSIKSPK KTKLLPKALS EDDVVISLEH IEEYGNVKWV ELRNKALLVL IYASGLRISE ALSITKLHLQ NLEFIRIIGK GSKERIIPWL PIAKNLITQY LEILPYKLGD NEPIFRGKQG KKLQPPVFNR ELIKLKHFYG LPQHLTAHSF RHSFASHLLE HGADLRSLQE LLXHKSLSTT QNYTKTSIKH LEAXYTTAYX XKNXXVR

Validated NT Sequence

ttnnttaact ttaagaagga ganataccat ggctcaccac caccaccacc atatgttaga tacatcaatt caagcattaa taaataagtg gcaaaaatac ctcgctttgc aaagaaatta ctctaatcat acagtaattt cttataataa tgaccttaag cattttcttg agtttatgaa ttattataat tcagagcttg taacgataaa tcatattaaa accgcagata tcagattaat ccgaagttgg cttgcaaaac gtaactgtga taattttacc gcttcttcaa tttcacgtgg tttatctgca gtaaaaaatt tttataggtt tttagagaaa acaacacaat taaatagtca tataattttt tctataaaat ctcctaaaaa gactaaattg ttaccaaaag ctttatcgga agatgatgta gtaatatcac ttgaacatat tgaagaatac gggaatgtta agtgggtaga acttagaaat aaagccctac ttgtccttat atatgcttca ggtctacgta tatcggaagc attatcaatt acgaaacttc atctacaaaa tctagaattt ataagaataa taggtaaagg tagtaaagaa agaataattc cgtggttacc gattgctaaa aatttaatta cccaatattt agaaatattg ccgtataagt taggtgataa tgaacccata tttagaggaa agcagggtaa gaaattgcaa ccaccggtat ttaatcgtga attaattaaa ttaaagcatt tttatggttt accgcagcat ttaactgctc attcatttag acatagtttt gcttcacatt tgcttgagca tggtgcagat ttacgctctc ttcaagaact attangtcat aaaagtctat ctactacaca aaactatact aaaacnagta taaagcattt agaagctgna tatactaccg catatcnnnt aaaaaatnan ngagtaagat agnannnnnn nnnnnnnnnn nnnnnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttag atacatcaat tcaagcatta ataaataagt ggcaaaaata cctcgctttg caaagaaatt actctaatca tacagtaatt tcttataata atgaccttaa gcattttctt gagtttatga attattataa ttcagagctt gtaacgataa atcatattaa aaccgcagat atcagattaa tccgaagttg gcttgcaaaa cgtaactgtg ataattttac cgcttcttca atttcacgtg gtttatctgc agtaaaaaat ttttataggt ttttagagaa aacaacacaa ttaaatagtc atataatttt ttctataaaa tctcctaaaa agactaaatt gttaccaaaa gctttatcgg aagatgatgt agtaatatca cttgaacata ttgaagaata cgggaatgtt aagtgggtag aacttagaaa taaagcccta cttgtcctta tatatgcttc aggtctacgt atatcggaag cattatcaat tacgaaactt catctacaaa atctagaatt tataagaata ataggtaaag gtagtaaaga aagaataatt ccgtggttac cgattgctaa aaatttaatt acccaatatt tagaaatatt gccgtataag ttaggtgata atgaacccat atttagagga aagcagggta agaaattgca accaccggta tttaatcgtg aattaattaa attaaagcat ttttatggtt taccgcagca tttaactgct cattcattta gacatagttt tgcttcacat ttgcttgagc atggtgcaga tttacgctct cttcaagaac tattaggtca taaaagtcta tctactacac aaaactatac taaaacaagt ataaagcatt tagaagctgt atatactacc gcatatccaa ttaaaaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat