RicoA.00145.a

Aspartyl-tRNA synthetase

CENTER ID: RicoA.00145.a
ORGANISM: Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE: Boutonneuse fever
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.00145.a.B1.GE35273 Full length( RicoA.00145.a ) 1 602
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272944.4.peg.216
RefSeq: NP_359824.1
UniProt: Q92J82

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHKYRTHNCN ELQISDVETE VKLSGWVHRR RDHGNLVFID LRDHYGITQI VFTDQNPQLM EDASRLRYES VITVRGTVVA RSEDTINNTL PTGHVEVLAV EFSVESAADT LPFVINTEKD APEESRLKHR FLDLRREKLH NNIILRSQII SHIRHLMTAS GFTEFQTPIL TASSPEGARD FLVPSRMHPG KFYALPQAPQ QFKQLLMVSG FDRYFQIAPC FRDEDARADR SPGEFYQLDV EMSFVTQEDV FSTIEPVMYD LFTKFTDKKV SETPFVCIPY NESMLKYGSD KPDLRNPIII ADVTEIFRDS DFTIFRENIK KGSIVRAIPA PKAAALPRSF FDKMIEFAIS EGAGGLGYIQ FSETGEAKGP VVKFLSPQQL ESLKATASIS NGDAVFFASD KKEKAAKLAG KVRIRLGEEL DLLEKDCFKF CWITDFPFYE LNEETGKIDF SHNPFSMPQG GIDALEQAKT TEELLELTAY QYDIVCNGIE LSSGAIRNHK PEIMYKAFSI AGYSEEEVDK RFGGMIRAFK FGAPPHGGIA PGIDRIVMLL AEATNIREII AFPLNQQAED LLMNAPSYVE DKALKELSIM LSPSARKNAE KE
NT Sequence
ATGCATAAAT ACAGAACTCA TAATTGTAAC GAATTACAAA TATCCGATGT AGAAACAGAA GTTAAGTTAT CCGGTTGGGT ACATAGAAGA AGAGATCACG GGAATCTAGT TTTTATAGAT TTACGTGATC ATTACGGTAT AACTCAAATC GTGTTTACCG ATCAAAATCC TCAGCTTATG GAGGATGCAA GTCGTTTGCG TTATGAGTCG GTAATTACGG TAAGGGGTAC AGTTGTAGCA AGATCAGAGG ACACGATTAA TAATACGCTT CCGACAGGTC ATGTTGAAGT GCTAGCTGTG GAATTTAGTG TTGAGTCAGC TGCGGACACT CTACCTTTTG TTATTAATAC TGAAAAAGAC GCTCCCGAAG AGTCTAGGCT TAAACATCGC TTTTTAGATC TTAGGCGTGA GAAATTACAT AATAATATAA TACTCCGCTC ACAAATTATT TCTCATATTC GTCATTTGAT GACGGCAAGC GGTTTTACCG AATTTCAAAC TCCGATTTTA ACAGCAAGCT CACCTGAGGG GGCTAGGGAT TTTTTAGTAC CTAGTAGAAT GCATCCGGGT AAGTTCTATG CATTACCGCA AGCACCTCAG CAATTCAAGC AGTTATTGAT GGTATCAGGC TTTGATCGTT ATTTCCAAAT TGCTCCTTGT TTTCGGGATG AAGATGCAAG AGCCGATAGA TCACCCGGCG AGTTTTATCA GCTAGATGTA GAAATGTCGT TTGTTACACA GGAAGACGTA TTTAGTACTA TTGAGCCTGT GATGTATGAT TTATTCACTA AATTCACGGA TAAGAAAGTA TCAGAGACTC CTTTTGTTTG TATTCCATAT AATGAATCTA TGCTAAAATA CGGCTCTGAT AAGCCTGATT TGCGTAACCC TATTATAATT GCCGATGTAA CTGAGATATT TAGAGATTCC GATTTTACAA TCTTTAGAGA GAATATTAAA AAAGGTAGCA TAGTGCGTGC AATTCCTGCA CCTAAGGCAG CTGCACTGCC TCGTAGTTTC TTCGATAAAA TGATAGAGTT TGCGATATCA GAAGGAGCAG GTGGACTTGG CTATATTCAG TTTAGTGAAA CCGGTGAAGC AAAGGGACCA GTAGTAAAAT TCTTAAGCCC GCAGCAATTA GAGAGTCTAA AAGCTACAGC TAGTATTAGT AACGGTGATG CTGTATTTTT CGCTAGCGAT AAGAAAGAGA AAGCTGCTAA ACTTGCCGGT AAGGTTAGAA TAAGACTTGG TGAAGAGCTT GATTTACTTG AAAAAGATTG TTTTAAATTC TGCTGGATTA CCGATTTTCC ATTCTATGAG TTAAATGAAG AAACAGGAAA AATTGATTTT AGCCATAATC CGTTTTCTAT GCCGCAGGGC GGAATAGATG CTTTAGAGCA AGCTAAAACC ACTGAAGAGT TATTAGAACT TACCGCATAC CAATATGATA TTGTTTGTAA CGGTATTGAA CTGTCCAGCG GTGCTATTAG AAACCATAAG CCGGAAATTA TGTATAAAGC ATTTTCCATA GCAGGTTATA GTGAAGAGGA AGTTGATAAG AGATTTGGAG GGATGATTAG AGCATTTAAG TTTGGAGCTC CACCACATGG TGGAATAGCC CCCGGTATTG ACCGAATCGT TATGCTGCTT GCGGAAGCAA CAAATATAAG GGAAATAATT GCGTTTCCTT TAAACCAGCA GGCTGAAGAT TTACTAATGA ACGCCCCAAG CTATGTAGAG GATAAAGCTC TAAAAGAGCT AAGCATTATG CTCTCACCGT CTGCTAGAAA AAATGCAGAA AAAGAA
Details for RicoA.00145.a.B1.GE35273
HARVESTED ON: 5/1/2012
SEQUENCED ON: 5/2/2012
EXPECTED MW: 69kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 85
PERCENT COVERAGE: 61
Validated AA Sequence
MAHHHHHHMH KYRTHNCNEL QISDVETEVK LSGWVHRRRD HGNLVFIDLR DHYGITQIVF TDQNPQLMED ASRLRYESVI TVRGTVVARS EDTINNTLPT GHVEVLAVEF SVESAADTLP FVINTEKDAP EESRLKHRFL DLRREKLHNN IILRSQIISH IRHLMTASGF TEFQTPILTA SSPEGARDFL VPSRMHPGKF YALPQAPQQF KQLLMVSGFD RYFQIAPCFR DEDARADRSP GEFYQLDVEM SFVTQEDVFS TIEPVMYDLF TKFTDKKVSE TPFXXIPYNE SMLKYGSXKP DLRNPIIIAX VTXXFRDSXF TIFRXXXKKX AXXVXFXXXX AALXXXXXXX XXXXXXXXXX XXXXXSLXKX GXXKGXXX
Validated NT Sequence
tttnnnnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatgcataa atacagaact cataattgta acgaattaca aatatccgat gtagaaacag aagttaagtt atccggttgg gtacatagaa gaagagatca cgggaatcta gtttttatag atttacgtga tcattacggt ataactcaaa tcgtgtttac cgatcaaaat cctcagctta tggaggatgc aagtcgtttg cgttatgagt cggtaattac ggtaaggggt acagttgtag caagatcaga ggacacgatt aataatacgc ttccgacagg tcatgttgaa gtgctagctg tggaatttag tgttgagtca gctgcggaca ctctaccttt tgttattaat actgaaaaag acgctcccga agagtctagg cttaaacatc gctttttaga tcttaggcgt gagaaattac ataataatat aatactccgc tcacaaatta tttctcatat tcgtcatttg atgacggcaa gcggttttac cgaatttcaa actccgattt taacagcaag ctcacctgag ggggctaggg attttttagt acctagtaga atgcatccgg gtaagttcta tgcattaccg caagcacctc agcaattcaa gcagttattg atggtatcag gctttgatcg ttatttccaa attgctcctt gttttcggga tgaagatgca agagccgata gatcacccgg cgagttttat cagctagatg tagaaatgtc gtttgttaca caggaagacg tatttagtac tattgagcct gtgatgtatg atttattcac taaattcacg gataagaaag tatcagagac tccttttgnt tgnattccat ataatgaatc tatgctaaaa tacggctctg anaagcctga tttgcgtaac cctattataa ttgccnatgt aactganana tttagagatt ccnattttac natctttaga ganatnntaa aaaaggnagc annntgngtg cnnttcnngn accnnnnngc tgcactncnn nnnanttnnn nnnanaangn nnnanntngn nanntnnnan nnnncngnnn gnntnngnnn nnntcnttan nnaaancngg nnannnnaag ggnnnnnnnn nnnaa
Expected Protein Sequence
MAHHHHHHMH KYRTHNCNEL QISDVETEVK LSGWVHRRRD HGNLVFIDLR DHYGITQIVF TDQNPQLMED ASRLRYESVI TVRGTVVARS EDTINNTLPT GHVEVLAVEF SVESAADTLP FVINTEKDAP EESRLKHRFL DLRREKLHNN IILRSQIISH IRHLMTASGF TEFQTPILTA SSPEGARDFL VPSRMHPGKF YALPQAPQQF KQLLMVSGFD RYFQIAPCFR DEDARADRSP GEFYQLDVEM SFVTQEDVFS TIEPVMYDLF TKFTDKKVSE TPFVCIPYNE SMLKYGSDKP DLRNPIIIAD VTEIFRDSDF TIFRENIKKG SIVRAIPAPK AAALPRSFFD KMIEFAISEG AGGLGYIQFS ETGEAKGPVV KFLSPQQLES LKATASISNG DAVFFASDKK EKAAKLAGKV RIRLGEELDL LEKDCFKFCW ITDFPFYELN EETGKIDFSH NPFSMPQGGI DALEQAKTTE ELLELTAYQY DIVCNGIELS SGAIRNHKPE IMYKAFSIAG YSEEEVDKRF GGMIRAFKFG APPHGGIAPG IDRIVMLLAE ATNIREIIAF PLNQQAEDLL MNAPSYVEDK ALKELSIMLS PSARKNAEKE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcata aatacagaac tcataattgt aacgaattac aaatatccga tgtagaaaca gaagttaagt tatccggttg ggtacataga agaagagatc acgggaatct agtttttata gatttacgtg atcattacgg tataactcaa atcgtgttta ccgatcaaaa tcctcagctt atggaggatg caagtcgttt gcgttatgag tcggtaatta cggtaagggg tacagttgta gcaagatcag aggacacgat taataatacg cttccgacag gtcatgttga agtgctagct gtggaattta gtgttgagtc agctgcggac actctacctt ttgttattaa tactgaaaaa gacgctcccg aagagtctag gcttaaacat cgctttttag atcttaggcg tgagaaatta cataataata taatactccg ctcacaaatt atttctcata ttcgtcattt gatgacggca agcggtttta ccgaatttca aactccgatt ttaacagcaa gctcacctga gggggctagg gattttttag tacctagtag aatgcatccg ggtaagttct atgcattacc gcaagcacct cagcaattca agcagttatt gatggtatca ggctttgatc gttatttcca aattgctcct tgttttcggg atgaagatgc aagagccgat agatcacccg gcgagtttta tcagctagat gtagaaatgt cgtttgttac acaggaagac gtatttagta ctattgagcc tgtgatgtat gatttattca ctaaattcac ggataagaaa gtatcagaga ctccttttgt ttgtattcca tataatgaat ctatgctaaa atacggctct gataagcctg atttgcgtaa ccctattata attgccgatg taactgagat atttagagat tccgatttta caatctttag agagaatatt aaaaaaggta gcatagtgcg tgcaattcct gcacctaagg cagctgcact gcctcgtagt ttcttcgata aaatgataga gtttgcgata tcagaaggag caggtggact tggctatatt cagtttagtg aaaccggtga agcaaaggga ccagtagtaa aattcttaag cccgcagcaa ttagagagtc taaaagctac agctagtatt agtaacggtg atgctgtatt tttcgctagc gataagaaag agaaagctgc taaacttgcc ggtaaggtta gaataagact tggtgaagag cttgatttac ttgaaaaaga ttgttttaaa ttctgctgga ttaccgattt tccattctat gagttaaatg aagaaacagg aaaaattgat tttagccata atccgttttc tatgccgcag ggcggaatag atgctttaga gcaagctaaa accactgaag agttattaga acttaccgca taccaatatg atattgtttg taacggtatt gaactgtcca gcggtgctat tagaaaccat aagccggaaa ttatgtataa agcattttcc atagcaggtt atagtgaaga ggaagttgat aagagatttg gagggatgat tagagcattt aagtttggag ctccaccaca tggtggaata gcccccggta ttgaccgaat cgttatgctg cttgcggaag caacaaatat aagggaaata attgcgtttc ctttaaacca gcaggctgaa gatttactaa tgaacgcccc aagctatgta gaggataaag ctctaaaaga gctaagcatt atgctctcac cgtctgctag aaaaaatgca gaaaaagaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat