SSGCID: Target Page

RicoA.00075.a

Delta-aminolevulinic acid dehydratase

CENTER ID:	RicoA.00075.a
ORGANISM:	Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE:	Boutonneuse fever
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RicoA.00075.a.B1.GE35268	Full length( RicoA.00075.a )		1	333

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RicoA.00075.a.B1.PW35886	Full length( RicoA.00075.a )		1	333

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272944.4.peg.907
RefSeq:	NP_360435.1
UniProt:	Q92HH3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNFMYPHIRL RRNRKAFWLR ELISESNLSV SDLVLPLFVV EGHNERQEIK TMPGIYRLSI DQIVETAKEA AELGINVIAL FPSIDQSLKS ENADEAYNLD NLICRTIRSI KNANIDIGII CDVALDPYTT HGHDGIVHHG EVDNDRSVRA LCNQALVLAK AGVDIVAPSD MMDGRIRAIR EYLDKEGFIN VGILAYAAKY ASSFYGPFRD AVKSNKKNYL DKSSYQMDVR NIKEAMLEIE HDIAEGADMV MVKPGMPFLD VIREAANKLN AKIFAYQVSG EYAMLKFAAE AGAMDWERAL MESLISFKRA GATGIFTYAA LEVAEILKNN KIK

NT Sequence

ATGAATTTTA TGTACCCCCA TATTAGACTT AGAAGAAATA GAAAAGCTTT TTGGCTTAGA GAGTTAATAT CCGAAAGTAA TTTATCGGTT AGTGATTTAG TGTTGCCTTT ATTTGTTGTT GAAGGACATA ATGAAAGGCA AGAAATTAAA ACTATGCCTG GTATATATCG TTTATCGATT GATCAAATAG TAGAAACGGC AAAAGAAGCA GCAGAGCTGG GTATTAATGT TATTGCATTA TTTCCGAGTA TTGATCAAAG TCTAAAAAGT GAAAATGCCG ATGAGGCTTA TAATTTAGAT AATTTAATAT GTAGAACTAT TAGAAGCATC AAAAACGCTA ATATCGATAT TGGTATAATA TGCGATGTAG CACTTGATCC TTATACTACA CATGGTCATG ATGGTATTGT GCATCATGGG GAAGTTGATA ATGATAGATC AGTAAGAGCT TTGTGTAATC AGGCATTAGT GCTAGCAAAA GCTGGAGTAG ATATTGTTGC ACCTTCCGAT ATGATGGATG GAAGAATCAG GGCTATAAGG GAATATCTTG ACAAAGAGGG ATTTATAAAT GTTGGAATTC TTGCTTATGC CGCTAAATAT GCTTCAAGTT TTTACGGTCC GTTTCGCGAT GCTGTTAAGA GTAATAAAAA GAATTATTTA GATAAATCAA GCTATCAAAT GGATGTACGT AATATTAAGG AAGCAATGCT TGAGATTGAG CATGATATAG CAGAGGGAGC CGATATGGTA ATGGTGAAGC CTGGTATGCC GTTTTTGGAT GTTATTCGTG AAGCGGCAAA TAAGTTAAAT GCTAAAATTT TTGCATATCA GGTTAGTGGA GAATATGCAA TGTTAAAATT TGCAGCAGAA GCAGGGGCAA TGGATTGGGA GAGAGCTTTG ATGGAGTCAT TAATTAGTTT TAAACGTGCC GGTGCCACAG GTATATTTAC CTATGCAGCT CTTGAAGTAG CTGAAATATT AAAGAACAAT AAAATTAAG

Details for RicoA.00075.a.B1.GE35268

HARVESTED ON:	5/1/2012
SEQUENCED ON:	5/2/2012
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

cttacttaat tttattgttc tttaatattt cagctacttc aagagctgca taggtaaata tacctgtggc accggcacgt ttaaaactaa ttaatgactc catcaaagct ctctcccaat ccattgcccc tgcttctgct gcaaatttta acattgcata ttctccacta acctgatatg caaaaatttt agcatttaac ttatttgccg cttcacgaat aacatccaaa aacggcatac caggcttcac cattaccata tcggctccct ctgctatatc atgctcaatc tcaagcattg cttccttaat attacgtaca tccatttgat agcttgattt atctaaataa ttctttttat tactcttaac agcatcgcga aacggaccgt aaaaacttga agcatattta gcggcataag caagaattcc aacatttata aatccctctt tgtcaagata ttcccttata gccctgattc ttccatccat catatcggaa ggtgcaacaa tatctactcc agcttttgct agcactaatg cctgattaca caaagctctt actgatctat cattatcaac ttccccatga tgcacaatac catcatgacc atgtgtagta taaggatcaa gtgctacatc gcatattata ccaatatcga tattagcgtt tttgatgctt ctaatagttc tacatattaa attatctaaa ttataagcct catcggcatt ttcacttttt agactttgat caatactcgg aaataatgca ataacattaa tacccagctc tgctgcttct tttgccgttt ctactatttg atcaatcgat aaacgatata taccaggcat agttttaatt tcttgccttt cattatgtcc ttcaacaaca aataaaggca acactaaatc actaaccgat aaattacttt cggatattaa ctctctaagc caaaaagctt ttctatttct tctaagtcta atatgggggt acataaaatt catatggtgg tggtggtggt gagcc

Expected Protein Sequence

MAHHHHHHMN FMYPHIRLRR NRKAFWLREL ISESNLSVSD LVLPLFVVEG HNERQEIKTM PGIYRLSIDQ IVETAKEAAE LGINVIALFP SIDQSLKSEN ADEAYNLDNL ICRTIRSIKN ANIDIGIICD VALDPYTTHG HDGIVHHGEV DNDRSVRALC NQALVLAKAG VDIVAPSDMM DGRIRAIREY LDKEGFINVG ILAYAAKYAS SFYGPFRDAV KSNKKNYLDK SSYQMDVRNI KEAMLEIEHD IAEGADMVMV KPGMPFLDVI REAANKLNAK IFAYQVSGEY AMLKFAAEAG AMDWERALME SLISFKRAGA TGIFTYAALE VAEILKNNKI K

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt ttatgtaccc ccatattaga cttagaagaa atagaaaagc tttttggctt agagagttaa tatccgaaag taatttatcg gttagtgatt tagtgttgcc tttatttgtt gttgaaggac ataatgaaag gcaagaaatt aaaactatgc ctggtatata tcgtttatcg attgatcaaa tagtagaaac ggcaaaagaa gcagcagagc tgggtattaa tgttattgca ttatttccga gtattgatca aagtctaaaa agtgaaaatg ccgatgaggc ttataattta gataatttaa tatgtagaac tattagaagc atcaaaaacg ctaatatcga tattggtata atatgcgatg tagcacttga tccttatact acacatggtc atgatggtat tgtgcatcat ggggaagttg ataatgatag atcagtaaga gctttgtgta atcaggcatt agtgctagca aaagctggag tagatattgt tgcaccttcc gatatgatgg atggaagaat cagggctata agggaatatc ttgacaaaga gggatttata aatgttggaa ttcttgctta tgccgctaaa tatgcttcaa gtttttacgg tccgtttcgc gatgctgtta agagtaataa aaagaattat ttagataaat caagctatca aatggatgta cgtaatatta aggaagcaat gcttgagatt gagcatgata tagcagaggg agccgatatg gtaatggtga agcctggtat gccgtttttg gatgttattc gtgaagcggc aaataagtta aatgctaaaa tttttgcata tcaggttagt ggagaatatg caatgttaaa atttgcagca gaagcagggg caatggattg ggagagagct ttgatggagt cattaattag ttttaaacgt gccggtgcca caggtatatt tacctatgca gctcttgaag tagctgaaat attaaagaac aataaaatta agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for RicoA.00075.a.B1.PW35886

PURIFICATION DATe:	6/1/2012
CONCENTRATION:	6.28mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATT TTATGTACCC CCATATTAGA CTTAGAAGAA ATAGAAAAGC TTTTTGGCTT AGAGAGTTAA TATCCGAAAG TAATTTATCG GTTAGTGATT TAGTGTTGCC TTTATTTGTT GTTGAAGGAC ATAATGAAAG GCAAGAAATT AAAACTATGC CTGGTATATA TCGTTTATCG ATTGATCAAA TAGTAGAAAC GGCAAAAGAA GCAGCAGAGC TGGGTATTAA TGTTATTGCA TTATTTCCGA GTATTGATCA AAGTCTAAAA AGTGAAAATG CCGATGAGGC TTATAATTTA GATAATTTAA TATGTAGAAC TATTAGAAGC ATCAAAAACG CTAATATCGA TATTGGTATA ATATGCGATG TAGCACTTGA TCCTTATACT ACACATGGTC ATGATGGTAT TGTGCATCAT GGGGAAGTTG ATAATGATAG ATCAGTAAGA GCTTTGTGTA ATCAGGCATT AGTGCTAGCA AAAGCTGGAG TAGATATTGT TGCACCTTCC GATATGATGG ATGGAAGAAT CAGGGCTATA AGGGAATATC TTGACAAAGA GGGATTTATA AATGTTGGAA TTCTTGCTTA TGCCGCTAAA TATGCTTCAA GTTTTTACGG TCCGTTTCGC GATGCTGTTA AGAGTAATAA AAAGAATTAT TTAGATAAAT CAAGCTATCA AATGGATGTA CGTAATATTA AGGAAGCAAT GCTTGAGATT GAGCATGATA TAGCAGAGGG AGCCGATATG GTAATGGTGA AGCCTGGTAT GCCGTTTTTG GATGTTATTC GTGAAGCGGC AAATAAGTTA AATGCTAAAA TTTTTGCATA TCAGGTTAGT GGAGAATATG CAATGTTAAA ATTTGCAGCA GAAGCAGGGG CAATGGATTG GGAGAGAGCT TTGATGGAGT CATTAATTAG TTTTAAACGT GCCGGTGCCA CAGGTATATT TACCTATGCA GCTCTTGAAG TAGCTGAAAT ATTAAAGAAC AATAAAATTA AGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT