RicoA.00069.a

Protein RecA

CENTER ID: RicoA.00069.a
ORGANISM: Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE: Boutonneuse fever
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.00069.a.B1.GE35267 Full length( RicoA.00069.a ) 1 343
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272944.4.peg.1359
RefSeq: NP_360819.1
UniProt: Q92GE1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNTDKERAI AAALAQIEKS YGKGSVMKLG QRPHVDIEAV STGSLGLDIA LGIGGVPKGR IIEIFGPESS GKTTLTLHLI AEAQKKGGTC AFIDAEHALD PAYAKKLGVN IDELIISQPD TGEQALEIAD TLIRSSGIDM IIIDSVAALV PKSEIEGEMG DAQMASQARL MSQALRKLTA SINRTNCITV FINQIRMKIG VMFGSPETTT GGNALKFYAS VRIDIRRIGS IKDKEEVIGS QTKVKVVKNK VSPPFKTADF DIMYGSGISK EGEIIDLGVK LDIVEKSGSW FSYKNVRIGQ GRENVKQYLK EHPQISNEIE KIIREKSSKI TNINLDQTEE END
NT Sequence
ATGTCAAATA CAGATAAGGA AAGAGCCATT GCCGCAGCAC TCGCACAAAT TGAAAAAAGC TACGGTAAAG GTTCGGTAAT GAAACTAGGG CAGCGTCCGC ATGTTGATAT AGAGGCCGTA TCAACCGGCT CACTTGGGCT AGATATAGCT CTTGGAATCG GCGGTGTTCC CAAAGGTAGA ATCATCGAAA TTTTTGGTCC TGAAAGCTCA GGTAAAACCA CTCTAACATT GCATTTAATT GCAGAAGCTC AAAAAAAGGG TGGTACATGT GCTTTTATTG ATGCCGAGCA TGCATTAGAC CCTGCTTATG CTAAAAAATT AGGCGTAAAT ATTGATGAGC TTATTATTTC ACAGCCCGAT ACAGGTGAAC AAGCCCTAGA AATTGCCGAT ACGTTAATTC GCTCCAGCGG TATTGATATG ATAATAATAG ATAGTGTTGC GGCTTTAGTA CCGAAATCAG AGATTGAGGG CGAAATGGGT GATGCTCAAA TGGCTTCTCA AGCAAGGTTA ATGAGCCAGG CTTTGCGTAA ACTTACTGCA TCAATTAATC GCACCAATTG TATTACTGTT TTCATCAATC AAATCAGAAT GAAAATAGGT GTGATGTTCG GTAGTCCTGA AACTACGACA GGCGGTAATG CCTTAAAATT CTATGCTTCT GTTAGGATCG ATATAAGAAG AATCGGCTCC ATTAAAGATA AAGAAGAAGT AATAGGCAGC CAAACCAAAG TAAAAGTAGT CAAAAACAAA GTATCTCCTC CATTTAAAAC TGCAGATTTT GATATAATGT ACGGTTCAGG TATTTCAAAA GAAGGCGAGA TAATCGACCT TGGAGTTAAG CTTGATATTG TTGAGAAATC CGGCTCTTGG TTTTCCTACA AGAACGTACG CATAGGTCAA GGGCGTGAAA ACGTCAAACA ATATTTAAAA GAACATCCAC AAATTTCCAA CGAAATTGAG AAAATAATAC GTGAAAAATC GTCAAAAATT ACTAATATAA ATCTTGATCA AACGGAGGAA GAAAATGAT
Details for RicoA.00069.a.B1.GE35267
HARVESTED ON: 5/1/2012
SEQUENCED ON: 5/2/2012
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MSNTDKERAI AAALAQIEKS YGKGSVMKLG QRPHVDIEAV STGSLGLDIA LGIGGVPKGR IIEIFGPESS GKTTLTLHLI AEAQKKGGTC AFIDAEHALD PAYAKKLGVN IDELIISQPD TGEQALEIAD TLIRSSGIDM IIIDSVAALV PKSEIEGEMG DAQMASQARL MSQALRKLTA SINRTNCITV FINQIRMKIG VMFGSPETTT GGNALKFYAS VRIDIRRIGS IKDKEEVIGS QTKVKVVKNK VSPPFKTADF DIMYGSGISK EGEIIDLGVK LDIVEKSGSW FSYKNVRIGQ GRENVKQYLK EHPQISNEIE KIIREKSSKI TNINLDQTE
Validated NT Sequence
ttcctccgtt tgatcaagat ttatattagt aatttttgac gatttttcac gtattatttt ctcaatttcg ttggaaattt gtggatgttc ttttaaatat tgtttgacgt tttcacgccc ttgacctatg cgtacgttct tgtaggaaaa ccaagagccg gatttctcaa caatatcaag cttaactcca aggtcgatta tctcgccttc ttttgaaata cctgaaccgt acattatatc aaaatctgca gttttaaatg gaggagatac tttgtttttg actactttta ctttggtttg gctgcctatt acttcttctt tatctttaat ggagccgatt cttcttatat cgatcctaac agaagcatag aattttaagg cattaccgcc tgtcgtagtt tcaggactac cgaacatcac acctattttc attctgattt gattgatgaa aacagtaata caattggtgc gattaattga tgcagtaagt ttacgcaaag cctggctcat taaccttgct tgagaagcca tttgagcatc acccatttcg ccctcaatct ctgatttcgg tactaaagcc gcaacactat ctattattat catatcaata ccgctggagc gaattaacgt atcggcaatt tctagggctt gttcacctgt atcgggctgt gaaataataa gctcatcaat atttacgcct aattttttag cataagcagg gtctaatgca tgctcggcat caataaaagc acatgtacca cccttttttt gagcttctgc aattaaatgc aatgttagag tggttttacc tgagctttca ggaccaaaaa tttcgatgat tctacctttg ggaacaccgc cgattccaag agctatatct agcccaagtg agccggttga tacggcctct atatcaacat gcggacgctg ccctagtttc attaccgaac ctttaccgta gcttttttca atttgtgcga gtgctgcggc aatggctctt tccttatctg tatttgacat atggtggtgg tggtggtggt g
Expected Protein Sequence
MAHHHHHHMS NTDKERAIAA ALAQIEKSYG KGSVMKLGQR PHVDIEAVST GSLGLDIALG IGGVPKGRII EIFGPESSGK TTLTLHLIAE AQKKGGTCAF IDAEHALDPA YAKKLGVNID ELIISQPDTG EQALEIADTL IRSSGIDMII IDSVAALVPK SEIEGEMGDA QMASQARLMS QALRKLTASI NRTNCITVFI NQIRMKIGVM FGSPETTTGG NALKFYASVR IDIRRIGSIK DKEEVIGSQT KVKVVKNKVS PPFKTADFDI MYGSGISKEG EIIDLGVKLD IVEKSGSWFS YKNVRIGQGR ENVKQYLKEH PQISNEIEKI IREKSSKITN INLDQTEEEN D
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcaa atacagataa ggaaagagcc attgccgcag cactcgcaca aattgaaaaa agctacggta aaggttcggt aatgaaacta gggcagcgtc cgcatgttga tatagaggcc gtatcaaccg gctcacttgg gctagatata gctcttggaa tcggcggtgt tcccaaaggt agaatcatcg aaatttttgg tcctgaaagc tcaggtaaaa ccactctaac attgcattta attgcagaag ctcaaaaaaa gggtggtaca tgtgctttta ttgatgccga gcatgcatta gaccctgctt atgctaaaaa attaggcgta aatattgatg agcttattat ttcacagccc gatacaggtg aacaagccct agaaattgcc gatacgttaa ttcgctccag cggtattgat atgataataa tagatagtgt tgcggcttta gtaccgaaat cagagattga gggcgaaatg ggtgatgctc aaatggcttc tcaagcaagg ttaatgagcc aggctttgcg taaacttact gcatcaatta atcgcaccaa ttgtattact gttttcatca atcaaatcag aatgaaaata ggtgtgatgt tcggtagtcc tgaaactacg acaggcggta atgccttaaa attctatgct tctgttagga tcgatataag aagaatcggc tccattaaag ataaagaaga agtaataggc agccaaacca aagtaaaagt agtcaaaaac aaagtatctc ctccatttaa aactgcagat tttgatataa tgtacggttc aggtatttca aaagaaggcg agataatcga ccttggagtt aagcttgata ttgttgagaa atccggctct tggttttcct acaagaacgt acgcataggt caagggcgtg aaaacgtcaa acaatattta aaagaacatc cacaaatttc caacgaaatt gagaaaataa tacgtgaaaa atcgtcaaaa attactaata taaatcttga tcaaacggag gaagaaaatg attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat