RicoA.00045.a

DNA polymerase III subunit epsilon

CENTER ID: RicoA.00045.a
ORGANISM: Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE: Boutonneuse fever
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.00045.a.B1.GE35264 Full length( RicoA.00045.a ) 1 230
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272944.4.peg.1281
RefSeq: NP_360749.1
UniProt: Q92GL1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSLREIILD TETTGLDPRQ GHRIVEIGAI EMVNKVLTGR NFHFYINPER DMPFEAYRIH GISGEFLKDK PLFHTIADDF LEFISDSKLI IHNAPFDIKF LNHELSLLKR TDIKLLELAN TIDTLVMARS IFPGSKYNLD ALCKRFKVDN SGRQLHGALK DAALLAEVYV ELMGGRQSAF KMVDKSAVIN NLATNQVNNK TEQTTIVIKP TKEELQKHKE FLSRILKTAY
NT Sequence
ATGTCGAGTT TAAGAGAAAT AATTTTAGAT ACCGAGACTA CGGGACTTGA CCCACGACAA GGTCATCGAA TCGTTGAGAT CGGTGCTATT GAAATGGTAA ATAAGGTATT AACAGGCAGG AATTTTCATT TTTATATTAA TCCTGAGCGA GATATGCCGT TTGAGGCTTA TAGAATTCAT GGTATATCCG GCGAATTTTT AAAAGACAAG CCTCTATTTC ATACAATAGC CGATGATTTT TTAGAGTTTA TTTCAGATAG CAAACTGATT ATTCATAATG CTCCTTTCGA TATTAAATTT CTAAATCACG AATTATCATT ATTAAAAAGA ACCGACATTA AACTCTTGGA ACTAGCAAAT ACTATTGATA CTTTAGTAAT GGCTAGAAGC ATATTTCCAG GCTCAAAATA TAATCTTGAT GCATTATGTA AAAGATTTAA AGTTGATAAT TCAGGTAGGC AGCTTCACGG AGCTTTGAAA GATGCCGCAT TACTTGCAGA AGTATATGTA GAATTAATGG GCGGTAGACA ATCCGCCTTT AAAATGGTTG ATAAATCTGC CGTAATAAAT AATTTAGCAA CTAATCAAGT AAATAACAAA ACAGAGCAAA CTACTATTGT TATTAAACCT ACAAAAGAAG AACTGCAAAA ACATAAAGAG TTTCTCAGTA GGATTTTAAA AACTGCTTAT
Details for RicoA.00045.a.B1.GE35264
HARVESTED ON: 5/1/2012
SEQUENCED ON: 5/2/2012
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 93
PERCENT COVERAGE: 70
Validated AA Sequence
MAHHHHHHMS SLREIILDTE TTGLDPRQGH RIVEIGAIEM VNKVLTGRNF HFYINPERDM PFEAYRIHGI SGEFLKDKPL FHTIADDFLE FISDSKLIIH NAPFDIKFLN HELSLLKRTD IKLLELANTI DTLVMARSIF PGSKYNLDAL CKXFKVXXXX XXXFXXL
Validated NT Sequence
ttnnttaact ttaagaagga ganataccat ggctcaccac caccaccacc atatgtcgag tttaagagaa ataattttag ataccgagac tacgggactt gacccacgac aaggtcatcg aatcgttgag atcggtgcta ttgaaatggt aaataaggta ttaacaggca ggaattttca tttttatatt aatcctgagc gagatatgcc gtttgaggct tatagaattc atggtatatc cggcgaattt ttaaaagaca agcctctatt tcatacaata gccgatgatt ttttagagtt tatttcagat agcaaactga ttattcataa tgctcctttc gatattaaat ttctaaatca cgaattatca ttattaaaaa gaaccgacat taaactcttg gaactagcaa atactattga tactttagta atggctagaa gcatatttcc aggctcaaaa tataatcttg atgcattatg taaaanattt aaagttgana nnnnnngngn nnncnncttc nngnancttt gaangatgcc gcattacttg cngaagtata tgtagaatta atgggcggta gacaatccgc ctttaaaatg gttgataaat ctgccgtaat aaataattta gcaactaanc aagtaaataa cnnaacnnag nnnantacta ttgtnntnaa ncctacnnnn naanannngc nnannnntaa nnagnttctc annatnnnaa anngcttatn nagtgannnn nnnnngatcn ngctgctana cccnaannnn nnnnnnnnnn nnnnnnannn nnnnnnnnnn nnnncnnnnn nnnnnnnng
Expected Protein Sequence
MAHHHHHHMS SLREIILDTE TTGLDPRQGH RIVEIGAIEM VNKVLTGRNF HFYINPERDM PFEAYRIHGI SGEFLKDKPL FHTIADDFLE FISDSKLIIH NAPFDIKFLN HELSLLKRTD IKLLELANTI DTLVMARSIF PGSKYNLDAL CKRFKVDNSG RQLHGALKDA ALLAEVYVEL MGGRQSAFKM VDKSAVINNL ATNQVNNKTE QTTIVIKPTK EELQKHKEFL SRILKTAY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcga gtttaagaga aataatttta gataccgaga ctacgggact tgacccacga caaggtcatc gaatcgttga gatcggtgct attgaaatgg taaataaggt attaacaggc aggaattttc atttttatat taatcctgag cgagatatgc cgtttgaggc ttatagaatt catggtatat ccggcgaatt tttaaaagac aagcctctat ttcatacaat agccgatgat tttttagagt ttatttcaga tagcaaactg attattcata atgctccttt cgatattaaa tttctaaatc acgaattatc attattaaaa agaaccgaca ttaaactctt ggaactagca aatactattg atactttagt aatggctaga agcatatttc caggctcaaa atataatctt gatgcattat gtaaaagatt taaagttgat aattcaggta ggcagcttca cggagctttg aaagatgccg cattacttgc agaagtatat gtagaattaa tgggcggtag acaatccgcc tttaaaatgg ttgataaatc tgccgtaata aataatttag caactaatca agtaaataac aaaacagagc aaactactat tgttattaaa cctacaaaag aagaactgca aaaacataaa gagtttctca gtaggatttt aaaaactgct tattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat