RicoA.00008.a

Serine hydroxymethyltransferase

CENTER ID: RicoA.00008.a
ORGANISM: Rickettsia conorii str. Malish 7
ASSOCIATED DISEASE: Boutonneuse fever
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.00008.a.B1.GE35263 Full length( RicoA.00008.a ) 1 420
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RicoA.00008.a.B1.PS01562 Full length( RicoA.00008.a ) 1 420 unavailable

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|272944.4.peg.1320
RefSeq: NP_360783.1
UniProt: Q92GH7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNIFNNNLHE TDKEINEIIK HEKLRQSSVI ELIASENFVS PAVLEAQGAL LTNKYAEGYP SKRFYNGCEE VDKAENLAIE RVKKLFNCKY ANVQPHSGSQ ANQAVYLALL QPGDTVLGMS LDSGGHLTHG AAPNMSGKWF NAVSYSVNKE TYLIDYDEIE RLADLHKPKL LIAGFSAYPR NIDFAKFREI VDKVGAYFMA DIAHIAGLVA TGEHQSPIPY AHAVTSTTHK TLRGPRGGLI LSNDEAIGHK INSALFPGLQ GGPLMHIIAA KAVAFLENLQ PEYKSYIQQV ISNAKALASS LQERGYDILT GGTDNHIVLV DLRKDGITGK LAANSLDRAG ITCNKNAIPF DETSPFITSG IRLGTPACTT RGFKEKDFVL VGHMVADILD GLKNNEDNSA LEQKVLNEVT KLIELFPFYG
NT Sequence
ATGAATATTT TTAATAATAA TTTACACGAA ACGGATAAAG AGATTAATGA AATAATAAAG CATGAAAAGC TACGTCAAAG TAGTGTAATC GAGCTTATTG CATCAGAAAA TTTCGTAAGT CCTGCTGTGC TTGAAGCTCA AGGGGCGCTT CTGACTAACA AATATGCCGA AGGATACCCA AGTAAGCGAT TCTATAACGG TTGCGAGGAA GTAGATAAAG CTGAAAATTT AGCTATAGAA CGAGTAAAAA AACTCTTTAA CTGCAAATAT GCAAATGTCC AACCTCATTC AGGATCACAA GCAAATCAGG CTGTATATCT TGCTTTATTA CAGCCGGGTG ATACGGTTCT TGGTATGTCC TTAGATAGCG GTGGGCATCT AACGCACGGT GCAGCTCCTA ATATGTCTGG TAAATGGTTT AACGCCGTTT CTTATAGCGT AAATAAAGAG ACTTATTTAA TCGATTATGA TGAGATTGAA CGCTTAGCGG ATTTGCATAA ACCAAAATTA CTTATAGCCG GTTTTTCTGC TTATCCTCGT AATATTGATT TTGCAAAATT TAGAGAAATC GTGGATAAAG TAGGAGCTTA CTTTATGGCA GATATTGCTC ATATTGCAGG ACTTGTTGCC ACAGGTGAGC ATCAAAGCCC TATTCCTTAT GCTCACGCTG TTACCTCTAC TACTCATAAA ACACTTAGAG GTCCAAGAGG CGGTTTAATT TTATCTAACG ATGAAGCGAT AGGTCACAAA ATAAATTCTG CATTATTTCC AGGACTACAA GGTGGTCCGT TAATGCATAT AATAGCTGCA AAAGCCGTAG CTTTTTTAGA AAATTTGCAG CCTGAATATA AAAGTTATAT TCAGCAGGTA ATAAGTAACG CTAAAGCTTT AGCAAGTAGC TTGCAAGAAA GAGGATATGA TATATTAACA GGTGGTACCG ATAATCATAT TGTTTTAGTG GATTTGCGTA AAGACGGAAT CACGGGTAAG CTTGCCGCAA ATTCTTTGGA TAGAGCAGGG ATTACGTGTA ATAAGAATGC TATCCCGTTT GATGAAACTT CGCCTTTTAT CACTTCAGGT ATTCGCCTTG GTACTCCCGC ATGTACTACT AGAGGTTTTA AAGAAAAGGA TTTTGTATTA GTCGGTCATA TGGTAGCAGA TATTTTAGAC GGCTTAAAAA ATAATGAAGA TAATAGCGCT CTTGAGCAGA AAGTATTAAA TGAAGTGACA AAATTAATTG AATTATTTCC GTTTTATGGC
Details for RicoA.00008.a.B1.GE35263
HARVESTED ON: 5/1/2012
SEQUENCED ON: 5/2/2012
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 67
Validated AA Sequence
MSLDSGGHLT HGAAPNMSGK WFNAVSYSVN KETYLIDYDE IERLADLHKP KLLIAGFSAY PRNIDFAKFR EIVDKVGAYF MADIAHIAGL VATGEHQSPI PYAHAVTSTT HKTLRGPRGG LILSNDEAIG HKINSALFPG LQGGPLMHII AAKAVAFLEN LQPEYKSYIQ QVISNAKALA SSLQERGYDI LTGGTDNHIV LVDLRKDGIT GKLAANSLDR AGITCNKNAI PFDETSPFIT SGIRLGTPAC TTRGFKEKDF VLVGHMVADI LDGLKNNEDN SALEQKVL
Validated NT Sequence
tttaatactt tctgctcaag agcgctatta tcttcattat tttttaagcc gtctaaaata tctgctacca tatgaccgac taatacaaaa tccttttctt taaaacctct agtagtacat gcgggagtac caaggcgaat acctgaagtg ataaaaggcg aagtttcatc aaacgggata gcattcttat tacacgtaat ccctgctcta tccaaagaat ttgcggcaag cttacccgtg attccgtctt tacgcaaatc cactaaaaca atatgattat cggtaccacc tgttaatata tcatatcctc tttcttgcaa gctacttgct aaagctttag cgttacttat tacctgctga atataacttt tatattcagg ctgcaaattt tctaaaaaag ctacggcttt tgcagctatt atatgcatta acggaccacc ttgtagtcct ggaaataatg cagaatttat tttgtgacct atcgcttcat cgttagataa aattaaaccg cctcttggac ctctaagtgt tttatgagta gtagaggtaa cagcgtgagc ataaggaata gggctttgat gctcacctgt ggcaacaagt cctgcaatat gagcaatatc tgccataaag taagctccta ctttatccac gatttctcta aattttgcaa aatcaatatt acgaggataa gcagaaaaac cggctataag taattttggt ttatgcaaat ccgctaagcg ttcaatctca tcataatcga ttaaataagt ctctttattt acgctataag aaacggcgtt aaaccattta ccagacatat taggagctgc accgtgcgtt agatgcccac cgctatctaa ggacatacca agaaccgtat cacccggctg taataaagca agatatacag cctgatttgc ttgtgatcct gaatgaggtt ggacatttgc atatttgcag ttaaagagtt tttttactcg ttctatagct aaattttcag ctttatctac ttcctcgcaa ccgttataga atcgcttact tgggtatcct tcggcatatt tgttagtcag aagcgcccct tgagcttcaa gcacagcagg acttacgaaa ttttctgatg caataagctc gattacacta ctttgacgta gcttttcatg ctttattatt tcattaatct ctttatccgt ttcgtgtaaa ttattattaa aaa
Expected Protein Sequence
MAHHHHHHMN IFNNNLHETD KEINEIIKHE KLRQSSVIEL IASENFVSPA VLEAQGALLT NKYAEGYPSK RFYNGCEEVD KAENLAIERV KKLFNCKYAN VQPHSGSQAN QAVYLALLQP GDTVLGMSLD SGGHLTHGAA PNMSGKWFNA VSYSVNKETY LIDYDEIERL ADLHKPKLLI AGFSAYPRNI DFAKFREIVD KVGAYFMADI AHIAGLVATG EHQSPIPYAH AVTSTTHKTL RGPRGGLILS NDEAIGHKIN SALFPGLQGG PLMHIIAAKA VAFLENLQPE YKSYIQQVIS NAKALASSLQ ERGYDILTGG TDNHIVLVDL RKDGITGKLA ANSLDRAGIT CNKNAIPFDE TSPFITSGIR LGTPACTTRG FKEKDFVLVG HMVADILDGL KNNEDNSALE QKVLNEVTKL IELFPFYG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata tttttaataa taatttacac gaaacggata aagagattaa tgaaataata aagcatgaaa agctacgtca aagtagtgta atcgagctta ttgcatcaga aaatttcgta agtcctgctg tgcttgaagc tcaaggggcg cttctgacta acaaatatgc cgaaggatac ccaagtaagc gattctataa cggttgcgag gaagtagata aagctgaaaa tttagctata gaacgagtaa aaaaactctt taactgcaaa tatgcaaatg tccaacctca ttcaggatca caagcaaatc aggctgtata tcttgcttta ttacagccgg gtgatacggt tcttggtatg tccttagata gcggtgggca tctaacgcac ggtgcagctc ctaatatgtc tggtaaatgg tttaacgccg tttcttatag cgtaaataaa gagacttatt taatcgatta tgatgagatt gaacgcttag cggatttgca taaaccaaaa ttacttatag ccggtttttc tgcttatcct cgtaatattg attttgcaaa atttagagaa atcgtggata aagtaggagc ttactttatg gcagatattg ctcatattgc aggacttgtt gccacaggtg agcatcaaag ccctattcct tatgctcacg ctgttacctc tactactcat aaaacactta gaggtccaag aggcggttta attttatcta acgatgaagc gataggtcac aaaataaatt ctgcattatt tccaggacta caaggtggtc cgttaatgca tataatagct gcaaaagccg tagctttttt agaaaatttg cagcctgaat ataaaagtta tattcagcag gtaataagta acgctaaagc tttagcaagt agcttgcaag aaagaggata tgatatatta acaggtggta ccgataatca tattgtttta gtggatttgc gtaaagacgg aatcacgggt aagcttgccg caaattcttt ggatagagca gggattacgt gtaataagaa tgctatcccg tttgatgaaa cttcgccttt tatcacttca ggtattcgcc ttggtactcc cgcatgtact actagaggtt ttaaagaaaa ggattttgta ttagtcggtc atatggtagc agatatttta gacggcttaa aaaataatga agataatagc gctcttgagc agaaagtatt aaatgaagtg acaaaattaa ttgaattatt tccgttttat ggctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for RicoA.00008.a.B1.PS01562
PURIFICATION DATe: 7/23/2012
CONCENTRATION: 59.54mg/ml
OBSERVED MW: 43kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 67
Protocol Notes
notes unavailable
Validated AA Sequence
MSLDSGGHLT HGAAPNMSGK WFNAVSYSVN KETYLIDYDE IERLADLHKP KLLIAGFSAY PRNIDFAKFR EIVDKVGAYF MADIAHIAGL VATGEHQSPI PYAHAVTSTT HKTLRGPRGG LILSNDEAIG HKINSALFPG LQGGPLMHII AAKAVAFLEN LQPEYKSYIQ QVISNAKALA SSLQERGYDI LTGGTDNHIV LVDLRKDGIT GKLAANSLDR AGITCNKNAI PFDETSPFIT SGIRLGTPAC TTRGFKEKDF VLVGHMVADI LDGLKNNEDN SALEQKVL
Validated NT Sequence
tttaatactt tctgctcaag agcgctatta tcttcattat tttttaagcc gtctaaaata tctgctacca tatgaccgac taatacaaaa tccttttctt taaaacctct agtagtacat gcgggagtac caaggcgaat acctgaagtg ataaaaggcg aagtttcatc aaacgggata gcattcttat tacacgtaat ccctgctcta tccaaagaat ttgcggcaag cttacccgtg attccgtctt tacgcaaatc cactaaaaca atatgattat cggtaccacc tgttaatata tcatatcctc tttcttgcaa gctacttgct aaagctttag cgttacttat tacctgctga atataacttt tatattcagg ctgcaaattt tctaaaaaag ctacggcttt tgcagctatt atatgcatta acggaccacc ttgtagtcct ggaaataatg cagaatttat tttgtgacct atcgcttcat cgttagataa aattaaaccg cctcttggac ctctaagtgt tttatgagta gtagaggtaa cagcgtgagc ataaggaata gggctttgat gctcacctgt ggcaacaagt cctgcaatat gagcaatatc tgccataaag taagctccta ctttatccac gatttctcta aattttgcaa aatcaatatt acgaggataa gcagaaaaac cggctataag taattttggt ttatgcaaat ccgctaagcg ttcaatctca tcataatcga ttaaataagt ctctttattt acgctataag aaacggcgtt aaaccattta ccagacatat taggagctgc accgtgcgtt agatgcccac cgctatctaa ggacatacca agaaccgtat cacccggctg taataaagca agatatacag cctgatttgc ttgtgatcct gaatgaggtt ggacatttgc atatttgcag ttaaagagtt tttttactcg ttctatagct aaattttcag ctttatctac ttcctcgcaa ccgttataga atcgcttact tgggtatcct tcggcatatt tgttagtcag aagcgcccct tgagcttcaa gcacagcagg acttacgaaa ttttctgatg caataagctc gattacacta ctttgacgta gcttttcatg ctttattatt tcattaatct ctttatccgt ttcgtgtaaa ttattattaa aaa
Expressed Protein Sequence
MAHHHHHHMN IFNNNLHETD KEINEIIKHE KLRQSSVIEL IASENFVSPA VLEAQGALLT NKYAEGYPSK RFYNGCEEVD KAENLAIERV KKLFNCKYAN VQPHSGSQAN QAVYLALLQP GDTVLGMSLD SGGHLTHGAA PNMSGKWFNA VSYSVNKETY LIDYDEIERL ADLHKPKLLI AGFSAYPRNI DFAKFREIVD KVGAYFMADI AHIAGLVATG EHQSPIPYAH AVTSTTHKTL RGPRGGLILS NDEAIGHKIN SALFPGLQGG PLMHIIAAKA VAFLENLQPE YKSYIQQVIS NAKALASSLQ ERGYDILTGG TDNHIVLVDL RKDGITGKLA ANSLDRAGIT CNKNAIPFDE TSPFITSGIR LGTPACTTRG FKEKDFVLVG HMVADILDGL KNNEDNSALE QKVLNEVTKL IELFPFYG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATA TTTTTAATAA TAATTTACAC GAAACGGATA AAGAGATTAA TGAAATAATA AAGCATGAAA AGCTACGTCA AAGTAGTGTA ATCGAGCTTA TTGCATCAGA AAATTTCGTA AGTCCTGCTG TGCTTGAAGC TCAAGGGGCG CTTCTGACTA ACAAATATGC CGAAGGATAC CCAAGTAAGC GATTCTATAA CGGTTGCGAG GAAGTAGATA AAGCTGAAAA TTTAGCTATA GAACGAGTAA AAAAACTCTT TAACTGCAAA TATGCAAATG TCCAACCTCA TTCAGGATCA CAAGCAAATC AGGCTGTATA TCTTGCTTTA TTACAGCCGG GTGATACGGT TCTTGGTATG TCCTTAGATA GCGGTGGGCA TCTAACGCAC GGTGCAGCTC CTAATATGTC TGGTAAATGG TTTAACGCCG TTTCTTATAG CGTAAATAAA GAGACTTATT TAATCGATTA TGATGAGATT GAACGCTTAG CGGATTTGCA TAAACCAAAA TTACTTATAG CCGGTTTTTC TGCTTATCCT CGTAATATTG ATTTTGCAAA ATTTAGAGAA ATCGTGGATA AAGTAGGAGC TTACTTTATG GCAGATATTG CTCATATTGC AGGACTTGTT GCCACAGGTG AGCATCAAAG CCCTATTCCT TATGCTCACG CTGTTACCTC TACTACTCAT AAAACACTTA GAGGTCCAAG AGGCGGTTTA ATTTTATCTA ACGATGAAGC GATAGGTCAC AAAATAAATT CTGCATTATT TCCAGGACTA CAAGGTGGTC CGTTAATGCA TATAATAGCT GCAAAAGCCG TAGCTTTTTT AGAAAATTTG CAGCCTGAAT ATAAAAGTTA TATTCAGCAG GTAATAAGTA ACGCTAAAGC TTTAGCAAGT AGCTTGCAAG AAAGAGGATA TGATATATTA ACAGGTGGTA CCGATAATCA TATTGTTTTA GTGGATTTGC GTAAAGACGG AATCACGGGT AAGCTTGCCG CAAATTCTTT GGATAGAGCA GGGATTACGT GTAATAAGAA TGCTATCCCG TTTGATGAAA CTTCGCCTTT TATCACTTCA GGTATTCGCC TTGGTACTCC CGCATGTACT ACTAGAGGTT TTAAAGAAAA GGATTTTGTA TTAGTCGGTC ATATGGTAGC AGATATTTTA GACGGCTTAA AAAATAATGA AGATAATAGC GCTCTTGAGC AGAAAGTATT AAATGAAGTG ACAAAATTAA TTGAATTATT TCCGTTTTAT GGCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT