RibeA.17987.a
DNA polymerase III subunit beta (EC 2.7.7.7)
| CENTER ID: | RibeA.17987.a |
| ORGANISM: | Rickettsia bellii RML369-C |
| ASSOCIATED DISEASE: | Typhus |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB/C |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RibeA.17987.a.B1.GE41037 | full length | 1 | 379 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RibeA.17987.a.B1.PW38223 | full length | 1 | 379 |
Structures
6MAN
DEPOSITED: 8/28/2018
DETERMINATION: XRay
CLONE: RibeA.17987.a.B1.GE41037
PROTEIN: RibeA.17987.a.B1.PW38223
Publications by SSGCID
Exquisite selectivity of griselimycin extends to beta subunit of DNA polymerases from Gram-negative bacterial pathogens
Abendroth JA, Barrett L, Choi R, Dranow DM, Edwards TE, Hewitt SN, Lorimer DD, Myler PJ, Phan IQ, Pierce PG, Staker BL, Subramanian S, Udell HS, Van Voorhis W, Wallace E, Mayclin SJ, Hammerson B, Chun IKH, Barrett KF, Conrady DG, Mueller R, Tillery L, Lukat P, Blankenfeldt W, Higgins T, Bowatte K, Smutney H, Stigliano M, Fenwick MK, Craig J, Peng V, DeRocher AE
Commun Biol - 2024
volume 7, issue 1,
PMID: 39639052; PMCID: PMC11621423
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|336407.7.peg.752 |
| UniProt: | Q1RIS7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKVIVETKT LVQALGFASS VVEKRNIISE LANIKLLAKD GLLELSSTNM DLYLSQKIGV QVVSEGELTV STKTLNDIVK KLPDSELTLT DLGTTGLEIT GKNCRFNLFT LPVESFPVMD NINPEASFKI SCAEFAKIIE STKFSVSLDE TRYNLNGIYL HVKDSEFYAA STDGHRLSVS SVVLAEKIED FGVILPQKSA EEILKIVKDS KNANADIEIL LSSNKIKFIC NENVIMLSKL IDGTFPDYSS FIPENSSSKL VINRKIFADT IERIAIITVE KFRAVKLSLS GEALEISAIG EARGNAKEVI NSSKETENFY EYSGETNLDI GFNPQYLEDV LKAIKSDLVE LYFSSVSAPV LIKFPESPKD IFVVMPVKV
NT Sequence
atgttaaagg taatagttga gacaaaaaca ttagttcaag cacttggttt tgcaagttct gttgttgaaa aacgtaatat aatatccgag cttgcaaata ttaaattatt ggcaaaggat gggctgttag agcttagctc taccaatatg gacttatatt taagccaaaa aataggagtg caagtagtaa gtgagggtga gcttactgtc tccactaaaa ctttaaatga tatagtgaaa aagcttcctg attcagagct taccctaact gatttaggta ctacagggct tgaaataaca ggaaaaaatt gtcgctttaa tttatttact ctgccagtag agtcttttcc tgtgatggat aatattaatc cagaagctag ctttaaaatt tcttgtgccg agtttgctaa aataatcgaa tcaacaaaat tttcggtttc tttagatgaa actcgttata atctaaacgg catttatttg catgtaaaag atagcgagtt ttatgctgca agtactgatg ggcatagact ttctgtttca tctgtagtgc tagctgaaaa aatagaggat ttcggtgtta tattgccgca aaagagtgct gaagagattt taaagatagt aaaagattct aaaaatgcaa atgcggatat agaaatactt ttaagtagta acaaaattaa gttcatatgc aatgaaaatg ttattatgct ctcaaagctg attgatggta ctttccctga ttacagtagc tttattccag aaaatagcag ctcaaaacta gtaattaacc gcaaaatatt tgccgatact atcgagcgaa tagcgataat aacagttgaa aagttcagag ctgtgaaact atcactatcc ggtgaagctc tagagatcag tgcaataggg gaagcacgag gaaatgcaaa agaagttatt aattcttcaa aagagacaga aaatttttac gagtatagcg gtgaaactaa tttagatata ggtttcaatc cgcaatattt agaagatgtt ctaaaggcta taaaatcaga tttagtagag ctatatttct caagcgtttc agctcctgta cttattaaat tccctgaaag ccctaaggat atatttgtcg tcatgcccgt taaggtatag
Details for RibeA.17987.a.B1.GE41037
| HARVESTED ON: | 1/4/2017 |
| SEQUENCED ON: | 1/5/2017 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHML KVIVETKTLV QALGFASSVV EKRNIISELA NIKLLAKDGL LELSSTNMDL YLSQKIGVQV VSEGELTVST KTLNDIVKKL PDSELTLTDL GTTGLEITGK NCRFNLFTLP VESFPVMDNI NPEASFKISC AEFAKIIEST KFSVSLDETR YNLNGIYLHV KDSEFYAAST DGHRLSVSSV VLAEKIEDFG VILPQKSAEE ILKIVKDSKN ANADIEILLS SNKIKFICNE NVIMLSKLID GTFPDYSSFI PENSSSKLVI NRKIFADTIE RIAIITVEKF RAVKLSLSGE ALEISAIGEA RGNAKEVINS SKETENFYEY SGETNLDIGF NPQYLEDVLK AIKSDLVELY FSSVSAPVLI KFPESPKDI
Validated NT Sequence
aatatatcct tagggctttc agggaattta ataagtacag gagctgaaac gcttgagaaa tatagctcta ctaaatctga ttttatagcc tttagaacat cttctaaata ttgcggattg aaacctatat ctaaattagt ttcaccgcta tactcgtaaa aattttctgt ctcttttgaa gaattaataa cttcttttgc atttcctcgt gcttccccta ttgcactgat ctctagagct tcaccggata gtgatagttt cacagctctg aacttttcaa ctgttattat cgctattcgc tcgatagtat cggcaaatat tttgcggtta attactagtt ttgagctgct attttctgga ataaagctac tgtaatcagg gaaagtacca tcaatcagct ttgagagcat aataacattt tcattgcata tgaacttaat tttgttacta cttaaaagta tttctatatc cgcatttgca tttttagaat cttttactat ctttaaaatc tcttcagcac tcttttgcgg caatataaca ccgaaatcct ctattttttc agctagcact acagatgaaa cagaaagtct atgcccatca gtacttgcag cataaaactc gctatctttt acatgcaaat aaatgccgtt tagattataa cgagtttcat ctaaagaaac cgaaaatttt gttgattcga ttattttagc aaactcggca caagaaattt taaagctagc ttctggatta atattatcca tcacaggaaa agactctact ggcagagtaa ataaattaaa gcgacaattt tttcctgtta tttcaagccc tgtagtacct aaatcagtta gggtaagctc tgaatcagga agctttttca ctatatcatt taaagtttta gtggagacag taagctcacc ctcacttact acttgcactc ctattttttg gcttaaatat aagtccatat tggtagagct aagctctaac agcccatcct ttgccaataa tttaatattt gcaagctcgg atattatatt acgtttttca acaacagaac ttgcaaaacc aagtgcttga actaatgttt ttgtctcaac tattaccttt aacatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHML KVIVETKTLV QALGFASSVV EKRNIISELA NIKLLAKDGL LELSSTNMDL YLSQKIGVQV VSEGELTVST KTLNDIVKKL PDSELTLTDL GTTGLEITGK NCRFNLFTLP VESFPVMDNI NPEASFKISC AEFAKIIEST KFSVSLDETR YNLNGIYLHV KDSEFYAAST DGHRLSVSSV VLAEKIEDFG VILPQKSAEE ILKIVKDSKN ANADIEILLS SNKIKFICNE NVIMLSKLID GTFPDYSSFI PENSSSKLVI NRKIFADTIE RIAIITVEKF RAVKLSLSGE ALEISAIGEA RGNAKEVINS SKETENFYEY SGETNLDIGF NPQYLEDVLK AIKSDLVELY FSSVSAPVLI KFPESPKDIF VVMPVKV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttaa aggtaatagt tgagacaaaa acattagttc aagcacttgg ttttgcaagt tctgttgttg aaaaacgtaa tataatatcc gagcttgcaa atattaaatt attggcaaag gatgggctgt tagagcttag ctctaccaat atggacttat atttaagcca aaaaatagga gtgcaagtag taagtgaggg tgagcttact gtctccacta aaactttaaa tgatatagtg aaaaagcttc ctgattcaga gcttacccta actgatttag gtactacagg gcttgaaata acaggaaaaa attgtcgctt taatttattt actctgccag tagagtcttt tcctgtgatg gataatatta atccagaagc tagctttaaa atttcttgtg ccgagtttgc taaaataatc gaatcaacaa aattttcggt ttctttagat gaaactcgtt ataatctaaa cggcatttat ttgcatgtaa aagatagcga gttttatgct gcaagtactg atgggcatag actttctgtt tcatctgtag tgctagctga aaaaatagag gatttcggtg ttatattgcc gcaaaagagt gctgaagaga ttttaaagat agtaaaagat tctaaaaatg caaatgcgga tatagaaata cttttaagta gtaacaaaat taagttcata tgcaatgaaa atgttattat gctctcaaag ctgattgatg gtactttccc tgattacagt agctttattc cagaaaatag cagctcaaaa ctagtaatta accgcaaaat atttgccgat actatcgagc gaatagcgat aataacagtt gaaaagttca gagctgtgaa actatcacta tccggtgaag ctctagagat cagtgcaata ggggaagcac gaggaaatgc aaaagaagtt attaattctt caaaagagac agaaaatttt tacgagtata gcggtgaaac taatttagat ataggtttca atccgcaata tttagaagat gttctaaagg ctataaaatc agatttagta gagctatatt tctcaagcgt ttcagctcct gtacttatta aattccctga aagccctaag gatatatttg tcgtcatgcc cgttaaggta tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for RibeA.17987.a.B1.PW38223
| PURIFICATION DATe: | 3/13/2017 |
| CONCENTRATION: | 35.6mg/ml |
| OBSERVED MW: | 38kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML KVIVETKTLV QALGFASSVV EKRNIISELA NIKLLAKDGL LELSSTNMDL YLSQKIGVQV VSEGELTVST KTLNDIVKKL PDSELTLTDL GTTGLEITGK NCRFNLFTLP VESFPVMDNI NPEASFKISC AEFAKIIEST KFSVSLDETR YNLNGIYLHV KDSEFYAAST DGHRLSVSSV VLAEKIEDFG VILPQKSAEE ILKIVKDSKN ANADIEILLS SNKIKFICNE NVIMLSKLID GTFPDYSSFI PENSSSKLVI NRKIFADTIE RIAIITVEKF RAVKLSLSGE ALEISAIGEA RGNAKEVINS SKETENFYEY SGETNLDIGF NPQYLEDVLK AIKSDLVELY FSSVSAPVLI KFPESPKDI
Validated NT Sequence
aatatatcct tagggctttc agggaattta ataagtacag gagctgaaac gcttgagaaa tatagctcta ctaaatctga ttttatagcc tttagaacat cttctaaata ttgcggattg aaacctatat ctaaattagt ttcaccgcta tactcgtaaa aattttctgt ctcttttgaa gaattaataa cttcttttgc atttcctcgt gcttccccta ttgcactgat ctctagagct tcaccggata gtgatagttt cacagctctg aacttttcaa ctgttattat cgctattcgc tcgatagtat cggcaaatat tttgcggtta attactagtt ttgagctgct attttctgga ataaagctac tgtaatcagg gaaagtacca tcaatcagct ttgagagcat aataacattt tcattgcata tgaacttaat tttgttacta cttaaaagta tttctatatc cgcatttgca tttttagaat cttttactat ctttaaaatc tcttcagcac tcttttgcgg caatataaca ccgaaatcct ctattttttc agctagcact acagatgaaa cagaaagtct atgcccatca gtacttgcag cataaaactc gctatctttt acatgcaaat aaatgccgtt tagattataa cgagtttcat ctaaagaaac cgaaaatttt gttgattcga ttattttagc aaactcggca caagaaattt taaagctagc ttctggatta atattatcca tcacaggaaa agactctact ggcagagtaa ataaattaaa gcgacaattt tttcctgtta tttcaagccc tgtagtacct aaatcagtta gggtaagctc tgaatcagga agctttttca ctatatcatt taaagtttta gtggagacag taagctcacc ctcacttact acttgcactc ctattttttg gcttaaatat aagtccatat tggtagagct aagctctaac agcccatcct ttgccaataa tttaatattt gcaagctcgg atattatatt acgtttttca acaacagaac ttgcaaaacc aagtgcttga actaatgttt ttgtctcaac tattaccttt aacatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHML KVIVETKTLV QALGFASSVV EKRNIISELA NIKLLAKDGL LELSSTNMDL YLSQKIGVQV VSEGELTVST KTLNDIVKKL PDSELTLTDL GTTGLEITGK NCRFNLFTLP VESFPVMDNI NPEASFKISC AEFAKIIEST KFSVSLDETR YNLNGIYLHV KDSEFYAAST DGHRLSVSSV VLAEKIEDFG VILPQKSAEE ILKIVKDSKN ANADIEILLS SNKIKFICNE NVIMLSKLID GTFPDYSSFI PENSSSKLVI NRKIFADTIE RIAIITVEKF RAVKLSLSGE ALEISAIGEA RGNAKEVINS SKETENFYEY SGETNLDIGF NPQYLEDVLK AIKSDLVELY FSSVSAPVLI KFPESPKDIF VVMPVKV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTAA AGGTAATAGT TGAGACAAAA ACATTAGTTC AAGCACTTGG TTTTGCAAGT TCTGTTGTTG AAAAACGTAA TATAATATCC GAGCTTGCAA ATATTAAATT ATTGGCAAAG GATGGGCTGT TAGAGCTTAG CTCTACCAAT ATGGACTTAT ATTTAAGCCA AAAAATAGGA GTGCAAGTAG TAAGTGAGGG TGAGCTTACT GTCTCCACTA AAACTTTAAA TGATATAGTG AAAAAGCTTC CTGATTCAGA GCTTACCCTA ACTGATTTAG GTACTACAGG GCTTGAAATA ACAGGAAAAA ATTGTCGCTT TAATTTATTT ACTCTGCCAG TAGAGTCTTT TCCTGTGATG GATAATATTA ATCCAGAAGC TAGCTTTAAA ATTTCTTGTG CCGAGTTTGC TAAAATAATC GAATCAACAA AATTTTCGGT TTCTTTAGAT GAAACTCGTT ATAATCTAAA CGGCATTTAT TTGCATGTAA AAGATAGCGA GTTTTATGCT GCAAGTACTG ATGGGCATAG ACTTTCTGTT TCATCTGTAG TGCTAGCTGA AAAAATAGAG GATTTCGGTG TTATATTGCC GCAAAAGAGT GCTGAAGAGA TTTTAAAGAT AGTAAAAGAT TCTAAAAATG CAAATGCGGA TATAGAAATA CTTTTAAGTA GTAACAAAAT TAAGTTCATA TGCAATGAAA ATGTTATTAT GCTCTCAAAG CTGATTGATG GTACTTTCCC TGATTACAGT AGCTTTATTC CAGAAAATAG CAGCTCAAAA CTAGTAATTA ACCGCAAAAT ATTTGCCGAT ACTATCGAGC GAATAGCGAT AATAACAGTT GAAAAGTTCA GAGCTGTGAA ACTATCACTA TCCGGTGAAG CTCTAGAGAT CAGTGCAATA GGGGAAGCAC GAGGAAATGC AAAAGAAGTT ATTAATTCTT CAAAAGAGAC AGAAAATTTT TACGAGTATA GCGGTGAAAC TAATTTAGAT ATAGGTTTCA ATCCGCAATA TTTAGAAGAT GTTCTAAAGG CTATAAAATC AGATTTAGTA GAGCTATATT TCTCAAGCGT TTCAGCTCCT GTACTTATTA AATTCCCTGA AAGCCCTAAG GATATATTTG TCGTCATGCC CGTTAAGGTA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT