RibeA.17834.a

Putative methyltransferase

CENTER ID: RibeA.17834.a
ORGANISM: Rickettsia bellii RML369-C
ASSOCIATED DISEASE: Typhus
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.17834.a.B1.GE35531 Full length( RibeA.17834.a ) 1 553
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.17834.a.B1.PS01617 Full length( RibeA.17834.a ) 1 553

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|336407.7.peg.91
RefSeq: YP_537254.1
UniProt: Q1RKE9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAKASNSSN LPNGHDKTTK EKHNTQPVIN GAIKHNTYDE VPYESYPYSF TNPFHLSTLA TLFGVDAPNV ETAKILELGC AAGGNLIPHA VLYPKAHFVG IDLSKVQIDE ANKTVKELGL KNIEFHHCSI LDIDDSLNKF DYIICHGVMS WVSKNVRDKI FDVCNKNLSK NGIAYISYNT LPGWNMVRTV RDMVLYHSSS FVNVRDKIAQ SRLLLDFVKD SLENSKTPYA EVLKTEAGLL SKQTDHYLRH DHLEEENAQF YFHEFMDEAR KHNLQYLADC NLSTMYLGNM PQKVVEQLKV VNDIVRTEQY MDFITNRRFR STLLCHNDVK INRNINNEDI MKFNMIFNVV PEKSLKEVDL NNSSESLAFF LNGNKDSNLT TASPYMKAIL YTFSEHLNNP LSFEKITEEA NKKLHGTKLN EIKAELLENA MKLVLQGYIN ITTQKHRETP ELNKPKTTKL VIHQAIHTPY MWVTNLKHEP VGVNFFEKLA IRYMDGKHDK KAIIDAVMGH VVKGEINLSK DGQKIEDQEV IRKQLEVLFM PMIDKFAANA LLV
NT Sequence
ATGTCTGCAA AAGCTAGTAA TTCTTCAAAT CTTCCTAATG GTCACGATAA AACAACGAAA GAAAAACATA ATACACAACC TGTTATTAAT GGGGCAATAA AGCATAATAC TTATGACGAA GTGCCGTATG AAAGCTATCC ATATTCCTTT ACTAATCCTT TTCATTTAAG CACTCTTGCG ACTCTTTTCG GTGTAGATGC CCCTAATGTG GAAACCGCAA AAATATTAGA GTTAGGTTGT GCTGCTGGTG GTAATTTAAT TCCTCATGCT GTTCTTTATC CAAAAGCTCA TTTTGTTGGT ATTGATTTAT CTAAAGTACA AATTGACGAA GCAAATAAAA CTGTAAAAGA ACTTGGACTG AAAAATATAG AATTTCATCA TTGTTCTATA CTTGATATTG ATGATTCTTT GAATAAATTT GATTATATAA TTTGTCACGG TGTAATGTCT TGGGTATCAA AAAATGTTAG AGATAAAATC TTTGATGTAT GTAATAAAAA CCTAAGCAAA AACGGTATAG CATATATCAG TTATAATACT TTACCTGGCT GGAATATGGT TCGTACGGTT AGAGATATGG TGCTTTATCA TTCTAGTTCA TTTGTAAATG TACGTGATAA AATAGCTCAG TCTAGATTAT TATTAGATTT TGTTAAAGAT AGCTTAGAGA ATTCAAAAAC ACCTTATGCT GAAGTATTAA AAACCGAAGC CGGATTATTA TCGAAACAAA CAGATCATTA TTTACGTCAT GATCATTTAG AAGAAGAAAA TGCTCAGTTT TACTTCCATG AATTTATGGA TGAAGCTAGA AAGCATAACT TACAATATTT AGCTGATTGT AACCTTTCTA CTATGTATTT AGGTAATATG CCACAAAAAG TGGTAGAGCA GTTAAAAGTC GTAAATGATA TAGTTAGAAC TGAACAATAT ATGGATTTTA TTACAAATCG TCGTTTTAGA TCTACTTTAT TATGTCATAA TGATGTAAAA ATCAATAGAA ACATTAATAA CGAAGATATA ATGAAATTTA ATATGATCTT TAACGTCGTT CCTGAAAAAT CGCTTAAAGA GGTTGATCTT AATAATTCTT CTGAAAGCTT AGCATTCTTT TTGAACGGCA ATAAAGATAG TAATTTGACA ACTGCCTCAC CCTACATGAA AGCTATTTTA TATACTTTTA GTGAGCATCT TAATAACCCT CTAAGTTTTG AGAAAATAAC CGAGGAAGCT AATAAAAAGC TACATGGTAC TAAATTAAAT GAAATAAAAG CTGAGCTTTT GGAAAACGCA ATGAAGTTAG TATTACAAGG TTACATTAAT ATTACTACTC AGAAACATAG AGAAACACCT GAGCTTAATA AGCCTAAAAC TACGAAGCTT GTAATACATC AAGCAATCCA TACTCCTTAT ATGTGGGTTA CAAATTTAAA ACATGAGCCA GTAGGGGTTA ATTTCTTTGA AAAACTTGCT ATTAGATATA TGGATGGTAA GCATGATAAG AAAGCTATTA TTGATGCAGT GATGGGTCAT GTAGTAAAAG GTGAAATAAA TTTAAGCAAA GACGGACAAA AAATAGAAGA TCAAGAAGTA ATACGTAAAC AACTCGAGGT CTTATTTATG CCAATGATTG ACAAGTTTGC CGCTAACGCT TTATTGGTT
Details for RibeA.17834.a.B1.GE35531
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 64kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 47
Validated AA Sequence
MYLGNMPQKV VEQLXVVNDI VRTEQYMDFI TNRRFRSTLL CHNDVKINRN INNEDIMKFN MIXNVVPEKS LKEVDLNNSS ESLAFFLNGN KDSNLTTASP YMKAILYTFS EHLNNPLSFE KITEEANKKL HGTKLNEIKA ELLENAMKLV LQGYINITTQ KHRETPELNK PKTTKLVIHQ AIHTPYMWVT NLKHEPVGVN FFEKLAIRYM DGKHDKKAII DAVMGHVVKG EINLSKDGQK IEDQEVIRKQ LEVLFMPMID KFAANALLV
Validated NT Sequence
atcttactca cttaaaccaa taaagcgtta gcggcaaact tgtcaatcat tggcataaat aagacctcga gttgtttacg tattacttct tgatcttcta ttttttgtcc gtctttgctt aaatttattt caccttttac tacatgaccc atcactgcat caataatagc tttcttatca tgcttaccat ccatatatct aatagcaagt ttttcaaaga aattaacccc tactggctca tgttttaaat ttgtaaccca catataagga gtatggattg cttgatgtat tacaagcttc gtagttttag gcttattaag ctcaggtgtt tctctatgtt tctgagtagt aatattaatg taaccttgta atactaactt cattgcgttt tccaaaagct cagcttttat ttcatttaat ttagtaccat gtagcttttt attagcttcc tcggttattt tctcaaaact tagagggtta ttaagatgct cactaaaagt atataaaata gctttcatgt agggtgaggc agttgtcaaa ttactatctt tattgccgtt caaaaagaat gctaagcttt cagaagaatt attaagatca acctctttaa gcgatttttc aggaacgacg ttnaagatca tattaaattt cattatatct tcgttattaa tgtttctatt gatttttaca tcattatgac ataataaagt agatctaaaa cgacgatttg taataaaatc catatattgt tcagttctaa ctatatcatt tacgacttnt aactgctcta ccactttttg tggcatatta cctaaataca tagtanaaag gttacnatca gctaaatatt gtaagttatg ctttctagct tcntccntna attcntgnnn gtaaaactga ncattttctn cttctaaatg atcatgacgt
Expected Protein Sequence
MAHHHHHHMS AKASNSSNLP NGHDKTTKEK HNTQPVINGA IKHNTYDEVP YESYPYSFTN PFHLSTLATL FGVDAPNVET AKILELGCAA GGNLIPHAVL YPKAHFVGID LSKVQIDEAN KTVKELGLKN IEFHHCSILD IDDSLNKFDY IICHGVMSWV SKNVRDKIFD VCNKNLSKNG IAYISYNTLP GWNMVRTVRD MVLYHSSSFV NVRDKIAQSR LLLDFVKDSL ENSKTPYAEV LKTEAGLLSK QTDHYLRHDH LEEENAQFYF HEFMDEARKH NLQYLADCNL STMYLGNMPQ KVVEQLKVVN DIVRTEQYMD FITNRRFRST LLCHNDVKIN RNINNEDIMK FNMIFNVVPE KSLKEVDLNN SSESLAFFLN GNKDSNLTTA SPYMKAILYT FSEHLNNPLS FEKITEEANK KLHGTKLNEI KAELLENAMK LVLQGYINIT TQKHRETPEL NKPKTTKLVI HQAIHTPYMW VTNLKHEPVG VNFFEKLAIR YMDGKHDKKA IIDAVMGHVV KGEINLSKDG QKIEDQEVIR KQLEVLFMPM IDKFAANALL V
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctg caaaagctag taattcttca aatcttccta atggtcacga taaaacaacg aaagaaaaac ataatacaca acctgttatt aatggggcaa taaagcataa tacttatgac gaagtgccgt atgaaagcta tccatattcc tttactaatc cttttcattt aagcactctt gcgactcttt tcggtgtaga tgcccctaat gtggaaaccg caaaaatatt agagttaggt tgtgctgctg gtggtaattt aattcctcat gctgttcttt atccaaaagc tcattttgtt ggtattgatt tatctaaagt acaaattgac gaagcaaata aaactgtaaa agaacttgga ctgaaaaata tagaatttca tcattgttct atacttgata ttgatgattc tttgaataaa tttgattata taatttgtca cggtgtaatg tcttgggtat caaaaaatgt tagagataaa atctttgatg tatgtaataa aaacctaagc aaaaacggta tagcatatat cagttataat actttacctg gctggaatat ggttcgtacg gttagagata tggtgcttta tcattctagt tcatttgtaa atgtacgtga taaaatagct cagtctagat tattattaga ttttgttaaa gatagcttag agaattcaaa aacaccttat gctgaagtat taaaaaccga agccggatta ttatcgaaac aaacagatca ttatttacgt catgatcatt tagaagaaga aaatgctcag ttttacttcc atgaatttat ggatgaagct agaaagcata acttacaata tttagctgat tgtaaccttt ctactatgta tttaggtaat atgccacaaa aagtggtaga gcagttaaaa gtcgtaaatg atatagttag aactgaacaa tatatggatt ttattacaaa tcgtcgtttt agatctactt tattatgtca taatgatgta aaaatcaata gaaacattaa taacgaagat ataatgaaat ttaatatgat ctttaacgtc gttcctgaaa aatcgcttaa agaggttgat cttaataatt cttctgaaag cttagcattc tttttgaacg gcaataaaga tagtaatttg acaactgcct caccctacat gaaagctatt ttatatactt ttagtgagca tcttaataac cctctaagtt ttgagaaaat aaccgaggaa gctaataaaa agctacatgg tactaaatta aatgaaataa aagctgagct tttggaaaac gcaatgaagt tagtattaca aggttacatt aatattacta ctcagaaaca tagagaaaca cctgagctta ataagcctaa aactacgaag cttgtaatac atcaagcaat ccatactcct tatatgtggg ttacaaattt aaaacatgag ccagtagggg ttaatttctt tgaaaaactt gctattagat atatggatgg taagcatgat aagaaagcta ttattgatgc agtgatgggt catgtagtaa aaggtgaaat aaatttaagc aaagacggac aaaaaataga agatcaagaa gtaatacgta aacaactcga ggtcttattt atgccaatga ttgacaagtt tgccgctaac gctttattgg tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for RibeA.17834.a.B1.PS01617
PURIFICATION DATe: 9/7/2012
CONCENTRATION: 28.34mg/ml
OBSERVED MW: 64kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 47
Protocol Notes
notes unavailable
Validated AA Sequence
MYLGNMPQKV VEQLXVVNDI VRTEQYMDFI TNRRFRSTLL CHNDVKINRN INNEDIMKFN MIXNVVPEKS LKEVDLNNSS ESLAFFLNGN KDSNLTTASP YMKAILYTFS EHLNNPLSFE KITEEANKKL HGTKLNEIKA ELLENAMKLV LQGYINITTQ KHRETPELNK PKTTKLVIHQ AIHTPYMWVT NLKHEPVGVN FFEKLAIRYM DGKHDKKAII DAVMGHVVKG EINLSKDGQK IEDQEVIRKQ LEVLFMPMID KFAANALLV
Validated NT Sequence
atcttactca cttaaaccaa taaagcgtta gcggcaaact tgtcaatcat tggcataaat aagacctcga gttgtttacg tattacttct tgatcttcta ttttttgtcc gtctttgctt aaatttattt caccttttac tacatgaccc atcactgcat caataatagc tttcttatca tgcttaccat ccatatatct aatagcaagt ttttcaaaga aattaacccc tactggctca tgttttaaat ttgtaaccca catataagga gtatggattg cttgatgtat tacaagcttc gtagttttag gcttattaag ctcaggtgtt tctctatgtt tctgagtagt aatattaatg taaccttgta atactaactt cattgcgttt tccaaaagct cagcttttat ttcatttaat ttagtaccat gtagcttttt attagcttcc tcggttattt tctcaaaact tagagggtta ttaagatgct cactaaaagt atataaaata gctttcatgt agggtgaggc agttgtcaaa ttactatctt tattgccgtt caaaaagaat gctaagcttt cagaagaatt attaagatca acctctttaa gcgatttttc aggaacgacg ttnaagatca tattaaattt cattatatct tcgttattaa tgtttctatt gatttttaca tcattatgac ataataaagt agatctaaaa cgacgatttg taataaaatc catatattgt tcagttctaa ctatatcatt tacgacttnt aactgctcta ccactttttg tggcatatta cctaaataca tagtanaaag gttacnatca gctaaatatt gtaagttatg ctttctagct tcntccntna attcntgnnn gtaaaactga ncattttctn cttctaaatg atcatgacgt
Expressed Protein Sequence
MAHHHHHHMS AKASNSSNLP NGHDKTTKEK HNTQPVINGA IKHNTYDEVP YESYPYSFTN PFHLSTLATL FGVDAPNVET AKILELGCAA GGNLIPHAVL YPKAHFVGID LSKVQIDEAN KTVKELGLKN IEFHHCSILD IDDSLNKFDY IICHGVMSWV SKNVRDKIFD VCNKNLSKNG IAYISYNTLP GWNMVRTVRD MVLYHSSSFV NVRDKIAQSR LLLDFVKDSL ENSKTPYAEV LKTEAGLLSK QTDHYLRHDH LEEENAQFYF HEFMDEARKH NLQYLADCNL STMYLGNMPQ KVVEQLKVVN DIVRTEQYMD FITNRRFRST LLCHNDVKIN RNINNEDIMK FNMIFNVVPE KSLKEVDLNN SSESLAFFLN GNKDSNLTTA SPYMKAILYT FSEHLNNPLS FEKITEEANK KLHGTKLNEI KAELLENAMK LVLQGYINIT TQKHRETPEL NKPKTTKLVI HQAIHTPYMW VTNLKHEPVG VNFFEKLAIR YMDGKHDKKA IIDAVMGHVV KGEINLSKDG QKIEDQEVIR KQLEVLFMPM IDKFAANALL V
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTG CAAAAGCTAG TAATTCTTCA AATCTTCCTA ATGGTCACGA TAAAACAACG AAAGAAAAAC ATAATACACA ACCTGTTATT AATGGGGCAA TAAAGCATAA TACTTATGAC GAAGTGCCGT ATGAAAGCTA TCCATATTCC TTTACTAATC CTTTTCATTT AAGCACTCTT GCGACTCTTT TCGGTGTAGA TGCCCCTAAT GTGGAAACCG CAAAAATATT AGAGTTAGGT TGTGCTGCTG GTGGTAATTT AATTCCTCAT GCTGTTCTTT ATCCAAAAGC TCATTTTGTT GGTATTGATT TATCTAAAGT ACAAATTGAC GAAGCAAATA AAACTGTAAA AGAACTTGGA CTGAAAAATA TAGAATTTCA TCATTGTTCT ATACTTGATA TTGATGATTC TTTGAATAAA TTTGATTATA TAATTTGTCA CGGTGTAATG TCTTGGGTAT CAAAAAATGT TAGAGATAAA ATCTTTGATG TATGTAATAA AAACCTAAGC AAAAACGGTA TAGCATATAT CAGTTATAAT ACTTTACCTG GCTGGAATAT GGTTCGTACG GTTAGAGATA TGGTGCTTTA TCATTCTAGT TCATTTGTAA ATGTACGTGA TAAAATAGCT CAGTCTAGAT TATTATTAGA TTTTGTTAAA GATAGCTTAG AGAATTCAAA AACACCTTAT GCTGAAGTAT TAAAAACCGA AGCCGGATTA TTATCGAAAC AAACAGATCA TTATTTACGT CATGATCATT TAGAAGAAGA AAATGCTCAG TTTTACTTCC ATGAATTTAT GGATGAAGCT AGAAAGCATA ACTTACAATA TTTAGCTGAT TGTAACCTTT CTACTATGTA TTTAGGTAAT ATGCCACAAA AAGTGGTAGA GCAGTTAAAA GTCGTAAATG ATATAGTTAG AACTGAACAA TATATGGATT TTATTACAAA TCGTCGTTTT AGATCTACTT TATTATGTCA TAATGATGTA AAAATCAATA GAAACATTAA TAACGAAGAT ATAATGAAAT TTAATATGAT CTTTAACGTC GTTCCTGAAA AATCGCTTAA AGAGGTTGAT CTTAATAATT CTTCTGAAAG CTTAGCATTC TTTTTGAACG GCAATAAAGA TAGTAATTTG ACAACTGCCT CACCCTACAT GAAAGCTATT TTATATACTT TTAGTGAGCA TCTTAATAAC CCTCTAAGTT TTGAGAAAAT AACCGAGGAA GCTAATAAAA AGCTACATGG TACTAAATTA AATGAAATAA AAGCTGAGCT TTTGGAAAAC GCAATGAAGT TAGTATTACA AGGTTACATT AATATTACTA CTCAGAAACA TAGAGAAACA CCTGAGCTTA ATAAGCCTAA AACTACGAAG CTTGTAATAC ATCAAGCAAT CCATACTCCT TATATGTGGG TTACAAATTT AAAACATGAG CCAGTAGGGG TTAATTTCTT TGAAAAACTT GCTATTAGAT ATATGGATGG TAAGCATGAT AAGAAAGCTA TTATTGATGC AGTGATGGGT CATGTAGTAA AAGGTGAAAT AAATTTAAGC AAAGACGGAC AAAAAATAGA AGATCAAGAA GTAATACGTA AACAACTCGA GGTCTTATTT ATGCCAATGA TTGACAAGTT TGCCGCTAAC GCTTTATTGG TTTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT