SSGCID: Target Page

RibeA.17669.a

dTDP-4-dehydrorhamnose reductase

CENTER ID:	RibeA.17669.a
ORGANISM:	Rickettsia bellii RML369-C
ASSOCIATED DISEASE:	Typhus
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RibeA.17669.a.B2.GE35526	Mature protein( RibeA.17669.a )		21	284

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|336407.7.peg.814
RefSeq:	YP_537880.1
UniProt:	Q1RIM3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKILILGATG MLGNSMVRFL STDKDLDVYA TSRSSSAHSY FSKDLANKIV PNVDVENYDS LVEVLNKIKP NIVINCIGLV KQLADANDPL KALPINSLLP HRLAGLCELV GSRLIHISTD CVFSGKKGNY KESDFPDCYD LYGRSKFLGE VDYPHAITLR TSIIGHELSG NRSLTNWFLS SEGSVKGFEK AIYSGFPTVE LARIIKNFVL PNKKLHGLYH VASKPINKLE LLKLIAEIYN KKIDIIPSDE LVIDRSLDAT HFNEITGYNP PEWKELVKRM CDFG

NT Sequence

ATGAAAATAT TGATTTTAGG TGCGACCGGC ATGCTTGGTA ATAGTATGGT TAGGTTTTTG AGCACTGACA AAGATCTTGA TGTTTATGCG ACCAGTCGGA GCAGCTCAGC TCATTCTTAT TTTTCTAAAG ATTTAGCAAA TAAAATAGTT CCAAATGTAG ATGTTGAGAA TTACGATTCG TTAGTTGAAG TACTAAATAA AATAAAGCCT AATATAGTAA TAAATTGTAT AGGGCTGGTA AAACAGCTCG CTGATGCAAA TGATCCTTTG AAAGCATTAC CGATTAATAG CTTACTGCCA CATAGGCTTG CTGGTTTATG TGAACTTGTC GGTAGCAGGT TAATCCATAT TAGTACTGAT TGTGTATTTT CAGGGAAAAA AGGCAATTAC AAGGAAAGTG ATTTTCCGGA TTGTTATGAT TTATATGGTC GTTCTAAATT TCTTGGTGAG GTGGATTATC CACATGCTAT TACGCTTCGT ACTTCAATTA TAGGTCATGA ACTTTCAGGA AATAGAAGCT TGACTAACTG GTTTTTAAGT TCAGAAGGGT CAGTAAAAGG TTTTGAAAAA GCTATATATT CAGGTTTTCC GACTGTAGAG CTTGCAAGAA TAATAAAGAA TTTTGTGCTA CCTAATAAAA AGCTTCACGG TCTTTACCAT GTCGCATCAA AGCCAATTAA TAAGTTAGAA TTATTAAAGC TAATAGCAGA AATATATAAT AAAAAAATCG ATATTATTCC GTCAGATGAG TTAGTAATAG ATCGCTCGCT AGACGCTACG CATTTTAATG AGATAACAGG TTATAATCCT CCTGAGTGGA AAGAGCTTGT GAAGCGTATG TGTGATTTTG GG

Details for RibeA.17669.a.B2.GE35526

HARVESTED ON:	6/12/2012
SEQUENCED ON:	6/12/2012
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	87
PERCENT COVERAGE:	97

Validated AA Sequence

MAXXHXHHMS TDKDLDVYAT SRSSSAHSYF SKDLANKIVP NVDVENYDSL VEVLNKIKPN IVINCIGLVK QLADANDPLK ALPINSLLPH RLAGLCELVG SRLIHISTDC VFSGKKGNYK ESDFPDCYDL YGRSKFLGEV DYPHAITLRT SIIGHELSGN RSLTNWFLSS XGSVKGFEKA IYSGFPTVXL ARIIKNFVLP NKKLHGLYHV XSKPINXLEL XKLIAXIXNK KIXIIPXXEL VIXXLARRXX FXXXXXXXLL XXKXX

Validated NT Sequence

annnnnnnnn tttancttta nnnnnnagan ataccatggc ncnncnccac cnccaccata tgagcactga caaagatctt gatgtttatg cgaccagtcg gagcagctca gctcattctt atttttctaa agatttagca aataaaatag ttccaaatgt agatgttgag aattacgatt cgttagttga agtactaaat aaaataaagc ctaatatagt aataaattgt atagggctgg taaaacagct cgctgatgca aatgatcctt tgaaagcatt accgattaat agcttactgc cacataggct tgctggttta tgtgaacttg tcggtagcag gttaatccat attagtactg attgtgtatt ttcagggaaa aaaggcaatt acaaggaaag tgattttccg gattgttatg atttatatgg tcgttctaaa tttcttggtg aggtggatta tccacatgct attacgcttc gtacttcaat tataggtcat gaactttcag gaaatagaag cttgactaac tggtttttaa gttcanaagg gtcagtaaaa ggntttgaaa aagctatata ttcaggtttt ccgactgtan agcttgcnag aataataaag aattttgtgc tacctaataa aaagcttcac ggtctttacc atgtcncatc aaagccaatt aatannttag aattatnnaa gctaatagca naaatatnta ataaaaaaat cganattatt ccnncnnang agttagtaat anntcnnctc gcnagacgcn ncncatttna tgnnnnacnn nnnntaancc tnctgaanng naaanactng tgaagcnnnn gngnganttt nnnnnnngnn nannannngn atcnnnnnaa nnnannnccg aang

Expected Protein Sequence

MAHHHHHHMS TDKDLDVYAT SRSSSAHSYF SKDLANKIVP NVDVENYDSL VEVLNKIKPN IVINCIGLVK QLADANDPLK ALPINSLLPH RLAGLCELVG SRLIHISTDC VFSGKKGNYK ESDFPDCYDL YGRSKFLGEV DYPHAITLRT SIIGHELSGN RSLTNWFLSS EGSVKGFEKA IYSGFPTVEL ARIIKNFVLP NKKLHGLYHV ASKPINKLEL LKLIAEIYNK KIDIIPSDEL VIDRSLDATH FNEITGYNPP EWKELVKRMC DFG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca ctgacaaaga tcttgatgtt tatgcgacca gtcggagcag ctcagctcat tcttattttt ctaaagattt agcaaataaa atagttccaa atgtagatgt tgagaattac gattcgttag ttgaagtact aaataaaata aagcctaata tagtaataaa ttgtataggg ctggtaaaac agctcgctga tgcaaatgat cctttgaaag cattaccgat taatagctta ctgccacata ggcttgctgg tttatgtgaa cttgtcggta gcaggttaat ccatattagt actgattgtg tattttcagg gaaaaaaggc aattacaagg aaagtgattt tccggattgt tatgatttat atggtcgttc taaatttctt ggtgaggtgg attatccaca tgctattacg cttcgtactt caattatagg tcatgaactt tcaggaaata gaagcttgac taactggttt ttaagttcag aagggtcagt aaaaggtttt gaaaaagcta tatattcagg ttttccgact gtagagcttg caagaataat aaagaatttt gtgctaccta ataaaaagct tcacggtctt taccatgtcg catcaaagcc aattaataag ttagaattat taaagctaat agcagaaata tataataaaa aaatcgatat tattccgtca gatgagttag taatagatcg ctcgctagac gctacgcatt ttaatgagat aacaggttat aatcctcctg agtggaaaga gcttgtgaag cgtatgtgtg attttgggtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat