RibeA.17347.a

Poly-beta-hydroxyalkanoate depolymerase

CENTER ID: RibeA.17347.a
ORGANISM: Rickettsia bellii RML369-C
ASSOCIATED DISEASE: Typhus
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.17347.a.B1.GE35535 Full length( RibeA.17347.a ) 1 425
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|336407.7.peg.576
RefSeq: YP_537675.1
UniProt: Q1RJ78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MINYDLNTNY TYYMLEGLRA QIAPMHLGIK ILKEAYERES LEENGFARIM HSYLTLCERM TRKYEKPEFN ILETTIDNKT YKINEKVILK KPFCELRHFE KVGFKSVLPK LLIVAPMAGH HATLLRSTVQ ELLPYTDVYI TDWTEANYVP LEAGSFDMDD YIDYVMEFIN FLGPNVHTMA VCQPTVPLLA AISLMSKDNS PNVPSSMILM GGPIDARKNP TAVNEFALSK SLEWFCKMVT MQVPPNYPGH GRKVYPGFLQ LAGFVSLNLF RHIDSHLELW QNLLNCDYQK ADHTIKFYDE YLAAMDMPAE FYLQTIDEVF QQFSLARGKL VSEQRPIDLK NITKCALLGI EGELDDIAAV GQTKAALKLC SNIPESMKKY HLQKGAGHYG VFSGSKFKQF IVPVIKSFIY DFDQANFKQP KAKMI
NT Sequence
ATGATTAACT ATGATTTAAA TACTAATTAT ACATACTATA TGCTGGAGGG GTTAAGAGCA CAAATAGCCC CTATGCATTT AGGCATAAAG ATTTTGAAAG AAGCATATGA ACGTGAATCA TTAGAGGAGA ATGGTTTTGC TAGAATCATG CATTCTTACT TAACACTTTG CGAGCGTATG ACTAGAAAAT ATGAAAAGCC GGAATTTAAT ATTCTTGAAA CTACTATTGA TAACAAAACC TATAAGATCA ACGAAAAAGT TATATTGAAA AAGCCATTTT GTGAGTTACG TCATTTTGAA AAAGTCGGCT TTAAAAGCGT CTTACCTAAA TTGTTAATAG TAGCTCCTAT GGCAGGTCAC CACGCTACGT TACTTCGCTC AACTGTACAG GAATTATTAC CATATACGGA TGTCTATATT ACCGATTGGA CGGAAGCAAA CTATGTACCG CTTGAAGCAG GTTCTTTTGA TATGGATGAT TACATTGATT ACGTAATGGA GTTTATTAAT TTTCTAGGAC CAAATGTTCA TACTATGGCT GTTTGTCAAC CGACTGTGCC TTTACTTGCT GCTATTAGTT TGATGTCGAA AGATAATAGC CCAAATGTGC CAAGCTCAAT GATTTTAATG GGTGGTCCTA TTGATGCTAG AAAAAATCCC ACCGCAGTAA ATGAATTTGC TTTAAGCAAA AGTTTGGAGT GGTTTTGTAA AATGGTTACG ATGCAAGTGC CACCTAATTA TCCTGGTCAT GGTAGAAAAG TATATCCAGG ATTTTTACAA TTAGCTGGTT TTGTAAGTCT AAATCTATTC CGTCATATAG ATTCGCATTT AGAATTATGG CAAAATTTAC TAAATTGTGA TTATCAAAAA GCTGATCATA CTATTAAATT CTATGATGAA TATTTAGCTG CTATGGATAT GCCAGCAGAA TTTTACCTAC AAACTATTGA TGAGGTATTT CAGCAATTTT CCCTAGCAAG AGGCAAATTA GTTTCAGAAC AACGTCCTAT TGATCTGAAA AATATCACTA AATGTGCATT ACTTGGAATT GAAGGAGAAC TTGACGATAT TGCAGCAGTT GGTCAAACCA AAGCAGCTTT AAAGCTTTGT AGTAATATTC CTGAATCAAT GAAGAAATAT CACTTACAAA AAGGTGCAGG ACATTATGGC GTATTTAGCG GCAGTAAGTT TAAACAGTTT ATAGTACCTG TTATTAAAAG CTTTATATAC GATTTTGACC AAGCTAATTT TAAACAACCT AAAGCTAAAA TGATT
Details for RibeA.17347.a.B1.GE35535
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 49kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 93
Validated AA Sequence
MLEGLRAQIA PMHLGIKILK EAYERESLEE NGFARIMHSY LTLCERMTRK YEKPEFNILE TTIDNKTYKI NEKVILKKPF CELRHFEKVG FKSVLPKLLI VAPMAGHHAT LLRSTVQELL PYTDVYITDW TEANYVPLEA GSFDMDDYID YVMEFINFLG PNVHTMAVCQ PTVPLLAAIS LMSKDNSPNV PSSMILMGGP IDARKNPTAV NEFALSKSLE WFCKMVTMQV PPNYPGHGRK VYPGFLQLAG FVSLNXFRHI DSHLELWQNL LNCDYQKADH TIKFYDEYLA AMDMPAEFYL QTIDEVFQQF SLARGKLVSE QRPIDLKNIT KCALLGIEGE LDDIAAVGQT KAALKLCSNI PESMKKYHLQ KGAGHYGVFS GSKFKQFIVP VIKSFIYDFD QAN
Validated NT Sequence
aattagcttg gtcaaaatcg tatataaagc ttttaataac aggtactata aactgtttaa acttactgcc gctaaatacg ccataatgtc ctgcaccttt ttgtaagtga tatttcttca ttgattcagg aatattacta caaagcttta aagctgcttt ggtttgacca actgctgcaa tatcgtcaag ttctccttca attccaagta atgcacattt agtgatattt ttcagatcaa taggacgttg ttctgaaact aatttgcctc ttgctaggga aaattgctga aatacctcat caatagtttg taggtaaaat tctgctggca tatccatagc agctaaatat tcatcataga atttaatagt atgatcagct ttttgataat cacaatttag taaattttgc cataattcta aatgcgaatc tatatgacgg aatanattta gacttacaaa accagctaat tgtaaaaatc ctggatatac ttttctacca tgaccaggat aattaggtgg cacttgcatc gtaaccattt tacaaaacca ctccaaactt ttgcttaaag caaattcatt tactgcggtg ggattttttc tagcatcaat aggaccaccc attaaaatca ttgagcttgg cacatttggg ctattatctt tcgacatcaa actaatagca gcaagtaaag gcacagtcgg ttgacaaaca gccatagtat gaacatttgg tcctagaaaa ttaataaact ccattacgta atcaatgtaa tcatccatat caaaagaacc tgcttcaagc ggtacatagt ttgcttccgt ccaatcggta atatagacat ccgtatatgg taataattcc tgtacagttg agcgaagtaa cgtagcgtgg tgacctgcca taggagctac tattaacaat ttaggtaaga cgcttttaaa gccgactttt tcaaaatgac gtaactcaca aaatggcttt ttcaatataa ctttttcgtt gatcttatag gttttgttat caatagtagt ttcaagaata ttaaattccg gcttttcata ttttctagtc atacgctcgc aaagtgttaa gtaagaatgc atgattctag caaaaccatt ctcctctaat gattcacgtt catatgcttc tttcaaaatc tttatgccta aatgcatagg ggctatttgt gctcttaacc cctccagcat atagtatgta taattagtat ttaaatcata gtt
Expected Protein Sequence
MAHHHHHHMI NYDLNTNYTY YMLEGLRAQI APMHLGIKIL KEAYERESLE ENGFARIMHS YLTLCERMTR KYEKPEFNIL ETTIDNKTYK INEKVILKKP FCELRHFEKV GFKSVLPKLL IVAPMAGHHA TLLRSTVQEL LPYTDVYITD WTEANYVPLE AGSFDMDDYI DYVMEFINFL GPNVHTMAVC QPTVPLLAAI SLMSKDNSPN VPSSMILMGG PIDARKNPTA VNEFALSKSL EWFCKMVTMQ VPPNYPGHGR KVYPGFLQLA GFVSLNLFRH IDSHLELWQN LLNCDYQKAD HTIKFYDEYL AAMDMPAEFY LQTIDEVFQQ FSLARGKLVS EQRPIDLKNI TKCALLGIEG ELDDIAAVGQ TKAALKLCSN IPESMKKYHL QKGAGHYGVF SGSKFKQFIV PVIKSFIYDF DQANFKQPKA KMI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatta actatgattt aaatactaat tatacatact atatgctgga ggggttaaga gcacaaatag cccctatgca tttaggcata aagattttga aagaagcata tgaacgtgaa tcattagagg agaatggttt tgctagaatc atgcattctt acttaacact ttgcgagcgt atgactagaa aatatgaaaa gccggaattt aatattcttg aaactactat tgataacaaa acctataaga tcaacgaaaa agttatattg aaaaagccat tttgtgagtt acgtcatttt gaaaaagtcg gctttaaaag cgtcttacct aaattgttaa tagtagctcc tatggcaggt caccacgcta cgttacttcg ctcaactgta caggaattat taccatatac ggatgtctat attaccgatt ggacggaagc aaactatgta ccgcttgaag caggttcttt tgatatggat gattacattg attacgtaat ggagtttatt aattttctag gaccaaatgt tcatactatg gctgtttgtc aaccgactgt gcctttactt gctgctatta gtttgatgtc gaaagataat agcccaaatg tgccaagctc aatgatttta atgggtggtc ctattgatgc tagaaaaaat cccaccgcag taaatgaatt tgctttaagc aaaagtttgg agtggttttg taaaatggtt acgatgcaag tgccacctaa ttatcctggt catggtagaa aagtatatcc aggattttta caattagctg gttttgtaag tctaaatcta ttccgtcata tagattcgca tttagaatta tggcaaaatt tactaaattg tgattatcaa aaagctgatc atactattaa attctatgat gaatatttag ctgctatgga tatgccagca gaattttacc tacaaactat tgatgaggta tttcagcaat tttccctagc aagaggcaaa ttagtttcag aacaacgtcc tattgatctg aaaaatatca ctaaatgtgc attacttgga attgaaggag aacttgacga tattgcagca gttggtcaaa ccaaagcagc tttaaagctt tgtagtaata ttcctgaatc aatgaagaaa tatcacttac aaaaaggtgc aggacattat ggcgtattta gcggcagtaa gtttaaacag tttatagtac ctgttattaa aagctttata tacgattttg accaagctaa ttttaaacaa cctaaagcta aaatgatttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat