SSGCID: Target Page

RibeA.17313.a

Putative oxygen-independent coproporphyrinogen III oxidase

CENTER ID:	RibeA.17313.a
ORGANISM:	Rickettsia bellii RML369-C
ASSOCIATED DISEASE:	Typhus
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RibeA.17313.a.B1.GE35561	Full length( RibeA.17313.a )		1	388

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RibeA.17313.a.B1.PW36166	Full length( RibeA.17313.a )		1	388

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|336407.7.peg.1292
RefSeq:	YP_538315.1
UniProt:	Q1RHD8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKEVKANDLS IYIHWPFCLS KCPYCDFNSH IANSIDDDNW LKSYEKEIEY FKDIIQNKYI RSIFFGGGTP SLMNPKIVEG IINKISGFAR IDKQTEITLE ANPTSFETEK FKAFKLAGIN RVSIGVQSLR ENDLKKLGRT HDSLQAIKTI ESASKIFPRV SFDLIYARSG QELKDWQQEL KQAMELVTGH ISLYQLTIEK GTPFYKLFHD GNLILPNSDL AAEMYEWTND YLESQKYFRY EISNYAKKGQ ECVHNLTYWN YNDYLGIGPG AHSRIIENPS SVSAIMMWHK PEKWLEAVNN KNNGVQTNVK LTKQEIIEEI LMMGLRLKDG INISALEQKI DAKLENILDI SSLNHYQELD LIRLDDNIYL TYKGLMLHSY IVPRLLQV

NT Sequence

ATGAAAGAAG TTAAGGCGAA TGATTTAAGC ATATATATTC ATTGGCCTTT TTGTTTGTCT AAATGTCCTT ATTGTGATTT TAATTCCCAT ATAGCGAATA GTATAGATGA TGATAATTGG CTTAAATCTT ATGAGAAAGA AATAGAATAT TTTAAAGATA TTATTCAAAA TAAATATATA AGATCTATTT TTTTTGGTGG CGGTACTCCC TCTTTGATGA ATCCCAAAAT TGTTGAGGGG ATAATAAATA AAATTAGTGG GTTTGCAAGA ATAGATAAGC AAACAGAAAT AACACTAGAG GCTAACCCAA CTTCATTTGA GACAGAGAAA TTCAAAGCAT TTAAGTTGGC AGGTATTAAT CGTGTTTCGA TTGGTGTACA ATCTTTAAGA GAGAATGACT TAAAAAAGCT TGGCAGAACT CATGATTCCT TGCAGGCTAT TAAAACGATT GAATCCGCTA GTAAAATTTT TCCTAGAGTT TCATTTGATC TAATATATGC TCGAAGTGGT CAGGAACTAA AAGATTGGCA GCAAGAACTA AAGCAAGCAA TGGAGCTTGT TACAGGGCAT ATTTCTCTTT ATCAGCTTAC TATTGAAAAA GGTACGCCGT TTTATAAATT ATTTCATGAT GGTAATTTAA TTCTTCCAAA TTCTGATCTG GCTGCTGAAA TGTATGAGTG GACGAATGAT TATTTAGAAT CTCAAAAATA TTTTAGATAT GAGATATCTA ATTATGCTAA AAAAGGACAG GAATGCGTAC ATAATTTAAC CTATTGGAAC TATAATGATT ATTTAGGTAT AGGTCCTGGT GCTCACAGTA GAATAATTGA AAATCCTAGT TCAGTATCCG CAATTATGAT GTGGCATAAG CCAGAAAAAT GGTTAGAAGC AGTTAATAAT AAGAATAATG GTGTTCAAAC CAATGTTAAG CTAACTAAGC AAGAAATTAT TGAAGAAATT TTGATGATGG GTTTACGCCT TAAGGATGGC ATAAATATTT CTGCATTGGA GCAGAAAATA GATGCAAAAT TAGAAAATAT TTTAGATATT AGCAGCCTTA ACCATTACCA AGAATTAGAT TTAATTAGGT TAGATGACAA TATATATCTA ACCTATAAGG GTTTAATGTT GCATAGTTAT ATAGTTCCAA GGCTTTTACA AGTC

Details for RibeA.17313.a.B1.GE35561

HARVESTED ON:	6/12/2012
SEQUENCED ON:	6/12/2012
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	77

Validated AA Sequence

MNPKIVEGII NKISGFARID KQTEITLEAN PTSFETEKFK AFKLAGINRV SIGVQSLREN DLKKLGRTHD SLQAIKTIES ASKIFPRVSF DLIYARSGQE LKDWQQELKQ AMELVTGHIS LYQLTIEKGT PFYKLFHDGN LILPNSDLAA EMYEWTNDYL ESQKYFRYEI SNYAKKGQEC VHNLTYWNYN DYLGIGPGAH SRIIENPSSV SAIMMWHKPE KWLEAVNNKN NGVQTNVKLT KQEIIEEILM MGLRLKDGIN ISALEQKIDA KLENILDISS LNHYQELDLI RLDDNIYLTY KGLMLH

Validated NT Sequence

actatgcaac attaaaccct tataggttag atatatattg tcatctaacc taattaaatc taattcttgg taatggttaa ggctgctaat atctaaaata ttttctaatt ttgcatctat tttctgctcc aatgcagaaa tatttatgcc atccttaagg cgtaaaccca tcatcaaaat ttcttcaata atttcttgct tagttagctt aacattggtt tgaacaccat tattcttatt attaactgct tctaaccatt tttctggctt atgccacatc ataattgcgg atactgaact aggattttca attattctac tgtgagcacc aggacctata cctaaataat cattatagtt ccaataggtt aaattatgta cgcattcctg tcctttttta gcataattag atatctcata tctaaaatat ttttgagatt ctaaataatc attcgtccac tcatacattt cagcagccag atcagaattt ggaagaatta aattaccatc atgaaataat ttataaaacg gcgtaccttt ttcaatagta agctgataaa gagaaatatg ccctgtaaca agctccattg cttgctttag ttcttgctgc caatctttta gttcctgacc acttcgagca tatattagat caaatgaaac tctaggaaaa attttactag cggattcaat cgttttaata gcctgcaagg aatcatgagt tctgccaagc ttttttaagt cattctctct taaagattgt acaccaatcg aaacacgatt aatacctgcc aacttaaatg ctttgaattt ctctgtctca aatgaagttg ggttagcctc tagtgttatt tctgtttgct tatctattct tgcaaaccca ctaattttat ttattatccc ctcaacaatt ttgggattca tcaaagaggg agtaccgcca ccaaaaaaaa tagatcttat atatttattt tgaataatat ctttaaaata ttctatttct ttctcataag atttaagcca attatcatca tctatactat tcgctatatg ggaattaaaa tcacaataag gacatttaga caaacaaaaa ggccaatgaa tatatatgct taaatcattc gccttaactt ctttc

Expected Protein Sequence

MAHHHHHHMK EVKANDLSIY IHWPFCLSKC PYCDFNSHIA NSIDDDNWLK SYEKEIEYFK DIIQNKYIRS IFFGGGTPSL MNPKIVEGII NKISGFARID KQTEITLEAN PTSFETEKFK AFKLAGINRV SIGVQSLREN DLKKLGRTHD SLQAIKTIES ASKIFPRVSF DLIYARSGQE LKDWQQELKQ AMELVTGHIS LYQLTIEKGT PFYKLFHDGN LILPNSDLAA EMYEWTNDYL ESQKYFRYEI SNYAKKGQEC VHNLTYWNYN DYLGIGPGAH SRIIENPSSV SAIMMWHKPE KWLEAVNNKN NGVQTNVKLT KQEIIEEILM MGLRLKDGIN ISALEQKIDA KLENILDISS LNHYQELDLI RLDDNIYLTY KGLMLHSYIV PRLLQV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag aagttaaggc gaatgattta agcatatata ttcattggcc tttttgtttg tctaaatgtc cttattgtga ttttaattcc catatagcga atagtataga tgatgataat tggcttaaat cttatgagaa agaaatagaa tattttaaag atattattca aaataaatat ataagatcta ttttttttgg tggcggtact ccctctttga tgaatcccaa aattgttgag gggataataa ataaaattag tgggtttgca agaatagata agcaaacaga aataacacta gaggctaacc caacttcatt tgagacagag aaattcaaag catttaagtt ggcaggtatt aatcgtgttt cgattggtgt acaatcttta agagagaatg acttaaaaaa gcttggcaga actcatgatt ccttgcaggc tattaaaacg attgaatccg ctagtaaaat ttttcctaga gtttcatttg atctaatata tgctcgaagt ggtcaggaac taaaagattg gcagcaagaa ctaaagcaag caatggagct tgttacaggg catatttctc tttatcagct tactattgaa aaaggtacgc cgttttataa attatttcat gatggtaatt taattcttcc aaattctgat ctggctgctg aaatgtatga gtggacgaat gattatttag aatctcaaaa atattttaga tatgagatat ctaattatgc taaaaaagga caggaatgcg tacataattt aacctattgg aactataatg attatttagg tataggtcct ggtgctcaca gtagaataat tgaaaatcct agttcagtat ccgcaattat gatgtggcat aagccagaaa aatggttaga agcagttaat aataagaata atggtgttca aaccaatgtt aagctaacta agcaagaaat tattgaagaa attttgatga tgggtttacg ccttaaggat ggcataaata tttctgcatt ggagcagaaa atagatgcaa aattagaaaa tattttagat attagcagcc ttaaccatta ccaagaatta gatttaatta ggttagatga caatatatat ctaacctata agggtttaat gttgcatagt tatatagttc caaggctttt acaagtctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for RibeA.17313.a.B1.PW36166

PURIFICATION DATe:	8/13/2012
CONCENTRATION:	2.98mg/ml
OBSERVED MW:	45kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	13
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	77

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAG AAGTTAAGGC GAATGATTTA AGCATATATA TTCATTGGCC TTTTTGTTTG TCTAAATGTC CTTATTGTGA TTTTAATTCC CATATAGCGA ATAGTATAGA TGATGATAAT TGGCTTAAAT CTTATGAGAA AGAAATAGAA TATTTTAAAG ATATTATTCA AAATAAATAT ATAAGATCTA TTTTTTTTGG TGGCGGTACT CCCTCTTTGA TGAATCCCAA AATTGTTGAG GGGATAATAA ATAAAATTAG TGGGTTTGCA AGAATAGATA AGCAAACAGA AATAACACTA GAGGCTAACC CAACTTCATT TGAGACAGAG AAATTCAAAG CATTTAAGTT GGCAGGTATT AATCGTGTTT CGATTGGTGT ACAATCTTTA AGAGAGAATG ACTTAAAAAA GCTTGGCAGA ACTCATGATT CCTTGCAGGC TATTAAAACG ATTGAATCCG CTAGTAAAAT TTTTCCTAGA GTTTCATTTG ATCTAATATA TGCTCGAAGT GGTCAGGAAC TAAAAGATTG GCAGCAAGAA CTAAAGCAAG CAATGGAGCT TGTTACAGGG CATATTTCTC TTTATCAGCT TACTATTGAA AAAGGTACGC CGTTTTATAA ATTATTTCAT GATGGTAATT TAATTCTTCC AAATTCTGAT CTGGCTGCTG AAATGTATGA GTGGACGAAT GATTATTTAG AATCTCAAAA ATATTTTAGA TATGAGATAT CTAATTATGC TAAAAAAGGA CAGGAATGCG TACATAATTT AACCTATTGG AACTATAATG ATTATTTAGG TATAGGTCCT GGTGCTCACA GTAGAATAAT TGAAAATCCT AGTTCAGTAT CCGCAATTAT GATGTGGCAT AAGCCAGAAA AATGGTTAGA AGCAGTTAAT AATAAGAATA ATGGTGTTCA AACCAATGTT AAGCTAACTA AGCAAGAAAT TATTGAAGAA ATTTTGATGA TGGGTTTACG CCTTAAGGAT GGCATAAATA TTTCTGCATT GGAGCAGAAA ATAGATGCAA AATTAGAAAA TATTTTAGAT ATTAGCAGCC TTAACCATTA CCAAGAATTA GATTTAATTA GGTTAGATGA CAATATATAT CTAACCTATA AGGGTTTAAT GTTGCATAGT TATATAGTTC CAAGGCTTTT ACAAGTCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT