RibeA.17300.a

Type I secretion outer membrane protein TolC

CENTER ID: RibeA.17300.a
ORGANISM: Rickettsia bellii RML369-C
ASSOCIATED DISEASE: Typhus
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.17300.a.B2.GE35558 Mature protein( RibeA.17300.a ) 19 454
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|336407.7.peg.268
RefSeq: YP_537404.1
UniProt: Q1RJZ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRKLTIFIFS LLLTSPVIAI DLQEALTEGY KNNNDLKTAR VKFVNSIEQF PQAFSGFMPS ASLSVNRNNS KTKYTNKRYA QLAGNPPEID NNQGALTIQQ SLFNGGSDVA ALRSAQAAFR ASRGQYYSSE QKVLLDLIGA YLDYFESKEK YDISESRVRT NIQQVNTVEE KLRLGEATEI DIATARAGLA AAETNKLTAY ADFQAKKANF IRVFGIEPTD IAMPILPQGL PNSLDELTKR AVNLNPEIDS AKHSVTSAKA QELVEKGRLL PQVSVQLQSG KTNYNPQNLN TQQINTRSVT TTLSVNVPIY PNGGAQYSKI RSAKNQTRNS AIQLDSVIRQ TQAYVISIWE GFEAAKSRII AADQGVAAAQ ISYDGTVQEE IVGSKTMLDV LSAEEKLYDA KITRVDAYKA SILSAYQMKS LTGELTAQSL KLKVKYFSPE EEFKTIKKKM FIGF
NT Sequence
ATGCGTAAGT TAACTATATT TATTTTCTCA TTATTACTAA CTTCTCCTGT TATTGCAATA GATTTGCAAG AAGCTTTAAC AGAAGGATAT AAAAATAACA ATGATCTGAA GACTGCAAGA GTTAAATTTG TTAATTCTAT TGAGCAATTT CCTCAAGCTT TTTCAGGATT TATGCCTAGT GCATCGTTGA GTGTTAACAG AAATAATAGT AAAACCAAAT ATACTAATAA AAGATATGCT CAATTAGCTG GTAACCCGCC AGAAATAGAC AATAATCAAG GAGCATTAAC AATTCAACAA TCTTTATTTA ATGGTGGTTC AGATGTTGCT GCTCTTAGAT CTGCACAAGC AGCTTTTAGA GCATCACGTG GTCAATATTA TTCTAGTGAG CAAAAAGTAT TATTAGACTT AATCGGTGCT TATCTTGATT ATTTTGAAAG TAAAGAGAAA TATGATATTT CAGAAAGTAG AGTTCGTACT AATATCCAAC AAGTAAACAC TGTAGAAGAA AAATTAAGAC TTGGTGAAGC AACCGAGATT GATATAGCAA CTGCAAGAGC AGGGCTTGCA GCAGCAGAAA CGAATAAGTT AACTGCTTAT GCTGATTTCC AAGCAAAAAA AGCAAACTTT ATAAGAGTAT TTGGTATAGA ACCTACTGAC ATAGCTATGC CTATTTTACC TCAAGGATTG CCAAATTCAT TAGATGAGCT AACAAAAAGA GCTGTTAATT TAAATCCTGA GATTGATTCA GCAAAACATA GCGTAACTTC AGCAAAGGCA CAAGAGTTAG TAGAAAAAGG AAGATTATTA CCGCAAGTAA GTGTGCAATT ACAATCTGGT AAAACTAATT ACAACCCACA AAATTTAAAT ACTCAACAAA TAAATACTAG AAGTGTTACT ACTACGTTAT CTGTTAATGT CCCTATTTAT CCAAATGGAG GAGCTCAATA TTCAAAAATT AGATCAGCTA AAAATCAAAC AAGAAATAGT GCAATACAGC TTGATAGTGT GATAAGACAA ACCCAGGCTT ATGTTATAAG CATATGGGAA GGGTTTGAAG CAGCAAAATC TCGTATTATT GCAGCGGATC AAGGAGTAGC GGCAGCTCAA ATATCCTATG ATGGTACAGT ACAAGAGGAA ATAGTAGGTT CTAAAACAAT GTTAGATGTT TTAAGTGCTG AAGAAAAACT CTATGATGCA AAAATAACAC GTGTTGATGC TTATAAAGCT TCTATACTTA GTGCATATCA AATGAAATCA TTAACTGGGG AGTTAACTGC TCAAAGTTTA AAGCTTAAGG TAAAATATTT CAGTCCTGAA GAGGAATTTA AGACTATTAA AAAGAAAATG TTTATAGGTT TC
Details for RibeA.17300.a.B2.GE35558
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 86
Validated AA Sequence
MPSASLSVNR NNSKTKYTNK RYAQLAGNPP EIDNNQGALT IQQSLFNGGS DVAALRSAQA AFRASRGQYY SSEQKVLLDL IGAYLDYFES KEKYDISESR VRTNIQQVNT VEEKLRLGEA TEIDIATARA GLAAAETNKL TAYADFQAKK ANFIRVFGIE PTDIAMPILP QGLPNSLDEL TKRAVNLNPE IDSAKHSVTS AKAQELVEKG RLLPQVSVQL XSGKTNYNPQ NLNTQQINTR SVTTTLSVNV PIYPNGGAQY SKIRSAKNQT RNSAIQLDSV IRQTQAYVIS IWEGFEAAKS RIIAADQGVA AAQISYDGTV QEEIVGSKTM LDVLSAEEKL YDAKITRVDA YKASILSAYQ MKSLTGELTA QSLKLKVKYF SPEEE
Validated NT Sequence
aattcctctt caggactgaa atattttacc ttaagcttta aactttgagc agttaactcc ccagttaatg atttcatttg atatgcacta agtatagaag ctttataagc atcaacacgt gttatttttg catcatagag tttttcttca gcacttaaaa catctaacat tgttttagaa cctactattt cctcttgtac tgtaccatca taggatattt gagctgccgc tactccttga tccgctgcaa taatacgaga ttttgctgct tcaaaccctt cccatatgct tataacataa gcctgggttt gtcttatcac actatcaagc tgtattgcac tatttcttgt ttgattttta gctgatctaa tttttgaata ttgagctcct ccatttggat aaatagggac attaacagat aacgtagtag taacacttct agtatttatt tgttgagtat ttaaattttg tgggttgtaa ttagttttac cagatngtaa ttgcacactt acttgcggta ataatcttcc tttttctact aactcttgtg cctttgctga agttacgcta tgttttgctg aatcaatctc aggatttaaa ttaacagctc tttttgttag ctcatctaat gaatttggca atccttgagg taaaataggc atagctatgt cagtaggttc tataccaaat actcttataa agtttgcttt ttttgcttgg aaatcagcat aagcagttaa cttattcgtt tctgctgctg caagccctgc tcttgcagtt gctatatcaa tctcggttgc ttcaccaagt cttaattttt cttctacagt gtttacttgt tggatattag tacgaactct actttctgaa atatcatatt tctctttact ttcaaaataa tcaagataag caccgattaa gtctaataat actttttgct cactagaata atattgacca cgtgatgctc taaaagctgc ttgtgcagat ctaagagcag caacatctga accaccatta aataaagatt gttgaattgt taatgctcct tgattattgt ctatttctgg cgggttacca gctaattgag catatctttt attagtatat ttggttttac tattatttct gttaacactc aacgatgcac taggcataaa tcctgaaaaa gcttgaggaa attgctcaat agaattaaca aatttaactc ttgcagtctt cagatcattg ttatttttat atccttctgt taaagcttct tgcaaatcta ttg
Expected Protein Sequence
MAHHHHHHMA IDLQEALTEG YKNNNDLKTA RVKFVNSIEQ FPQAFSGFMP SASLSVNRNN SKTKYTNKRY AQLAGNPPEI DNNQGALTIQ QSLFNGGSDV AALRSAQAAF RASRGQYYSS EQKVLLDLIG AYLDYFESKE KYDISESRVR TNIQQVNTVE EKLRLGEATE IDIATARAGL AAAETNKLTA YADFQAKKAN FIRVFGIEPT DIAMPILPQG LPNSLDELTK RAVNLNPEID SAKHSVTSAK AQELVEKGRL LPQVSVQLQS GKTNYNPQNL NTQQINTRSV TTTLSVNVPI YPNGGAQYSK IRSAKNQTRN SAIQLDSVIR QTQAYVISIW EGFEAAKSRI IAADQGVAAA QISYDGTVQE EIVGSKTMLD VLSAEEKLYD AKITRVDAYK ASILSAYQMK SLTGELTAQS LKLKVKYFSP EEEFKTIKKK MFIGF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa tagatttgca agaagcttta acagaaggat ataaaaataa caatgatctg aagactgcaa gagttaaatt tgttaattct attgagcaat ttcctcaagc tttttcagga tttatgccta gtgcatcgtt gagtgttaac agaaataata gtaaaaccaa atatactaat aaaagatatg ctcaattagc tggtaacccg ccagaaatag acaataatca aggagcatta acaattcaac aatctttatt taatggtggt tcagatgttg ctgctcttag atctgcacaa gcagctttta gagcatcacg tggtcaatat tattctagtg agcaaaaagt attattagac ttaatcggtg cttatcttga ttattttgaa agtaaagaga aatatgatat ttcagaaagt agagttcgta ctaatatcca acaagtaaac actgtagaag aaaaattaag acttggtgaa gcaaccgaga ttgatatagc aactgcaaga gcagggcttg cagcagcaga aacgaataag ttaactgctt atgctgattt ccaagcaaaa aaagcaaact ttataagagt atttggtata gaacctactg acatagctat gcctatttta cctcaaggat tgccaaattc attagatgag ctaacaaaaa gagctgttaa tttaaatcct gagattgatt cagcaaaaca tagcgtaact tcagcaaagg cacaagagtt agtagaaaaa ggaagattat taccgcaagt aagtgtgcaa ttacaatctg gtaaaactaa ttacaaccca caaaatttaa atactcaaca aataaatact agaagtgtta ctactacgtt atctgttaat gtccctattt atccaaatgg aggagctcaa tattcaaaaa ttagatcagc taaaaatcaa acaagaaata gtgcaataca gcttgatagt gtgataagac aaacccaggc ttatgttata agcatatggg aagggtttga agcagcaaaa tctcgtatta ttgcagcgga tcaaggagta gcggcagctc aaatatccta tgatggtaca gtacaagagg aaatagtagg ttctaaaaca atgttagatg ttttaagtgc tgaagaaaaa ctctatgatg caaaaataac acgtgttgat gcttataaag cttctatact tagtgcatat caaatgaaat cattaactgg ggagttaact gctcaaagtt taaagcttaa ggtaaaatat ttcagtcctg aagaggaatt taagactatt aaaaagaaaa tgtttatagg tttctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat