RibeA.10478.a

Probable branched-chain-amino-acid aminotransferase

CENTER ID: RibeA.10478.a
ORGANISM: Rickettsia bellii RML369-C
ASSOCIATED DISEASE: Typhus
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.10478.a.B1.GE35563 Full length( RibeA.10478.a ) 1 289
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.10478.a.B1.PW37355 Full length( RibeA.10478.a ) 1 289

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|336407.7.peg.850
RefSeq: YP_537911.1
UniProt: Q1RIJ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKNSENNLW YIWINGELVP YELATVHALT HSLHYSGSVF EGERAYNGKV FKLKEHTERL VKSAEVLGLK VPYNVEEIIK AHELLIEKNK IQDAYIRPLV WCGSESLNII NPKLSTNVLI AAVPSMPRAF AAGFNLYVSR WRKAAPNMMP VQSKSAAHYN MAITSKKEAK DLGYDDALLL DYEGYIAECT TTNIFFVKDN VLYTPIADRF LDGITRQTII EIAKNLGLEV KEERLKLEQI EDFISCFATG TAIEVQNINS IDIGNKKVIF NDHKIADVLK EEYGKIVRG
NT Sequence
ATGACAAAAA ATTCAGAAAA TAATCTTTGG TATATTTGGA TTAATGGTGA GTTAGTTCCT TATGAACTTG CTACAGTGCA TGCTTTAACT CATAGTTTGC ATTACTCAGG ATCAGTATTT GAGGGTGAGC GGGCTTATAA TGGGAAAGTT TTTAAACTTA AAGAACATAC TGAAAGATTG GTTAAATCAG CAGAAGTTTT AGGATTGAAA GTACCTTATA ATGTAGAAGA AATTATAAAA GCTCATGAGT TATTAATAGA GAAAAATAAG ATTCAGGATG CATATATAAG ACCGCTTGTT TGGTGTGGGA GTGAGTCTTT AAATATTATT AATCCTAAAT TGTCGACTAA CGTTTTGATT GCAGCTGTTC CTTCTATGCC ACGAGCATTT GCTGCAGGAT TTAATTTGTA TGTAAGCAGA TGGCGTAAAG CTGCACCTAA TATGATGCCG GTACAATCTA AATCCGCTGC TCATTATAAC ATGGCAATAA CAAGTAAGAA AGAGGCTAAA GACCTTGGCT ATGATGATGC ATTATTACTT GATTATGAGG GGTATATTGC GGAATGTACT ACAACAAATA TTTTCTTTGT TAAAGATAAT GTGCTATATA CTCCAATTGC TGATAGATTT TTAGATGGTA TAACGAGACA AACTATTATT GAAATTGCAA AAAATTTAGG ATTAGAAGTA AAAGAAGAAC GTCTAAAATT AGAACAGATA GAAGATTTTA TCAGCTGTTT TGCTACAGGT ACAGCAATTG AAGTGCAGAA TATTAATTCT ATAGATATTG GTAATAAGAA AGTTATTTTT AATGATCATA AAATTGCAGA TGTTTTGAAA GAAGAGTATG GTAAAATTGT GAGGGGG
Details for RibeA.10478.a.B1.GE35563
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT KNSENNLWYI WINGELVPYE LATVHALTHS LHYSGSVFEG ERAYNGKVFK LKEHTERLVK SAEVLGLKVP YNVEEIIKAH ELLIEKNKIQ DAYIRPLVWC GSESLNIINP KLSTNVLIAA VPSMPRAFAA GFNLYVSRWR KAAPNMMPVQ SKSAAHYNMA ITSKKEAKDL GYDDALLLDY EGYIAECTTT NIFFVKDNVL YTPIADRFLD GITRQTIIEI AKNLGLEVKE ERLKLEQIED FISCFATGTA IEVQNINSID IGNKKVIFND HKIADVLKEE YGKIVRG
Validated NT Sequence
ancttactca cttaccccct cacaatttta ccatactctt ctttcaaaac atctgcaatt ttatgatcat taaaaataac tttcttatta ccaatatcta tagaattaat attctgcact tcaattgctg tacctgtagc aaaacagctg ataaaatctt ctatctgttc taattttaga cgttcttctt ttacttctaa tcctaaattt tttgcaattt caataatagt ttgtctcgtt ataccatcta aaaatctatc agcaattgga gtatatagca cattatcttt aacaaagaaa atatttgttg tagtacattc cgcaatatac ccctcataat caagtaataa tgcatcatca tagccaaggt ctttagcctc tttcttactt gttattgcca tgttataatg agcagcggat ttagattgta ccggcatcat attaggtgca gctttacgcc atctgcttac atacaaatta aatcctgcag caaatgctcg tggcatagaa ggaacagctg caatcaaaac gttagtcgac aatttaggat taataatatt taaagactca ctcccacacc aaacaagcgg tcttatatat gcatcctgaa tcttattttt ctctattaat aactcatgag cttttataat ttcttctaca ttataaggta ctttcaatcc taaaacttct gctgatttaa ccaatctttc agtatgttct ttaagtttaa aaactttccc attataagcc cgctcaccct caaatactga tcctgagtaa tgcaaactat gagttaaagc atgcactgta gcaagttcat aaggaactaa ctcaccatta atccaaatat accaaagatt attttctgaa ttttttgtca tatggtggtg gtggtggtga gccatggtat
Expected Protein Sequence
MAHHHHHHMT KNSENNLWYI WINGELVPYE LATVHALTHS LHYSGSVFEG ERAYNGKVFK LKEHTERLVK SAEVLGLKVP YNVEEIIKAH ELLIEKNKIQ DAYIRPLVWC GSESLNIINP KLSTNVLIAA VPSMPRAFAA GFNLYVSRWR KAAPNMMPVQ SKSAAHYNMA ITSKKEAKDL GYDDALLLDY EGYIAECTTT NIFFVKDNVL YTPIADRFLD GITRQTIIEI AKNLGLEVKE ERLKLEQIED FISCFATGTA IEVQNINSID IGNKKVIFND HKIADVLKEE YGKIVRG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacaa aaaattcaga aaataatctt tggtatattt ggattaatgg tgagttagtt ccttatgaac ttgctacagt gcatgcttta actcatagtt tgcattactc aggatcagta tttgagggtg agcgggctta taatgggaaa gtttttaaac ttaaagaaca tactgaaaga ttggttaaat cagcagaagt tttaggattg aaagtacctt ataatgtaga agaaattata aaagctcatg agttattaat agagaaaaat aagattcagg atgcatatat aagaccgctt gtttggtgtg ggagtgagtc tttaaatatt attaatccta aattgtcgac taacgttttg attgcagctg ttccttctat gccacgagca tttgctgcag gatttaattt gtatgtaagc agatggcgta aagctgcacc taatatgatg ccggtacaat ctaaatccgc tgctcattat aacatggcaa taacaagtaa gaaagaggct aaagaccttg gctatgatga tgcattatta cttgattatg aggggtatat tgcggaatgt actacaacaa atattttctt tgttaaagat aatgtgctat atactccaat tgctgataga tttttagatg gtataacgag acaaactatt attgaaattg caaaaaattt aggattagaa gtaaaagaag aacgtctaaa attagaacag atagaagatt ttatcagctg ttttgctaca ggtacagcaa ttgaagtgca gaatattaat tctatagata ttggtaataa gaaagttatt tttaatgatc ataaaattgc agatgttttg aaagaagagt atggtaaaat tgtgaggggg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for RibeA.10478.a.B1.PW37355
PURIFICATION DATe: 9/3/2013
CONCENTRATION: 48.5mg/ml
OBSERVED MW: 33kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 21
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT KNSENNLWYI WINGELVPYE LATVHALTHS LHYSGSVFEG ERAYNGKVFK LKEHTERLVK SAEVLGLKVP YNVEEIIKAH ELLIEKNKIQ DAYIRPLVWC GSESLNIINP KLSTNVLIAA VPSMPRAFAA GFNLYVSRWR KAAPNMMPVQ SKSAAHYNMA ITSKKEAKDL GYDDALLLDY EGYIAECTTT NIFFVKDNVL YTPIADRFLD GITRQTIIEI AKNLGLEVKE ERLKLEQIED FISCFATGTA IEVQNINSID IGNKKVIFND HKIADVLKEE YGKIVRG
Validated NT Sequence
ancttactca cttaccccct cacaatttta ccatactctt ctttcaaaac atctgcaatt ttatgatcat taaaaataac tttcttatta ccaatatcta tagaattaat attctgcact tcaattgctg tacctgtagc aaaacagctg ataaaatctt ctatctgttc taattttaga cgttcttctt ttacttctaa tcctaaattt tttgcaattt caataatagt ttgtctcgtt ataccatcta aaaatctatc agcaattgga gtatatagca cattatcttt aacaaagaaa atatttgttg tagtacattc cgcaatatac ccctcataat caagtaataa tgcatcatca tagccaaggt ctttagcctc tttcttactt gttattgcca tgttataatg agcagcggat ttagattgta ccggcatcat attaggtgca gctttacgcc atctgcttac atacaaatta aatcctgcag caaatgctcg tggcatagaa ggaacagctg caatcaaaac gttagtcgac aatttaggat taataatatt taaagactca ctcccacacc aaacaagcgg tcttatatat gcatcctgaa tcttattttt ctctattaat aactcatgag cttttataat ttcttctaca ttataaggta ctttcaatcc taaaacttct gctgatttaa ccaatctttc agtatgttct ttaagtttaa aaactttccc attataagcc cgctcaccct caaatactga tcctgagtaa tgcaaactat gagttaaagc atgcactgta gcaagttcat aaggaactaa ctcaccatta atccaaatat accaaagatt attttctgaa ttttttgtca tatggtggtg gtggtggtga gccatggtat
Expressed Protein Sequence
MAHHHHHHMT KNSENNLWYI WINGELVPYE LATVHALTHS LHYSGSVFEG ERAYNGKVFK LKEHTERLVK SAEVLGLKVP YNVEEIIKAH ELLIEKNKIQ DAYIRPLVWC GSESLNIINP KLSTNVLIAA VPSMPRAFAA GFNLYVSRWR KAAPNMMPVQ SKSAAHYNMA ITSKKEAKDL GYDDALLLDY EGYIAECTTT NIFFVKDNVL YTPIADRFLD GITRQTIIEI AKNLGLEVKE ERLKLEQIED FISCFATGTA IEVQNINSID IGNKKVIFND HKIADVLKEE YGKIVRG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAA AAAATTCAGA AAATAATCTT TGGTATATTT GGATTAATGG TGAGTTAGTT CCTTATGAAC TTGCTACAGT GCATGCTTTA ACTCATAGTT TGCATTACTC AGGATCAGTA TTTGAGGGTG AGCGGGCTTA TAATGGGAAA GTTTTTAAAC TTAAAGAACA TACTGAAAGA TTGGTTAAAT CAGCAGAAGT TTTAGGATTG AAAGTACCTT ATAATGTAGA AGAAATTATA AAAGCTCATG AGTTATTAAT AGAGAAAAAT AAGATTCAGG ATGCATATAT AAGACCGCTT GTTTGGTGTG GGAGTGAGTC TTTAAATATT ATTAATCCTA AATTGTCGAC TAACGTTTTG ATTGCAGCTG TTCCTTCTAT GCCACGAGCA TTTGCTGCAG GATTTAATTT GTATGTAAGC AGATGGCGTA AAGCTGCACC TAATATGATG CCGGTACAAT CTAAATCCGC TGCTCATTAT AACATGGCAA TAACAAGTAA GAAAGAGGCT AAAGACCTTG GCTATGATGA TGCATTATTA CTTGATTATG AGGGGTATAT TGCGGAATGT ACTACAACAA ATATTTTCTT TGTTAAAGAT AATGTGCTAT ATACTCCAAT TGCTGATAGA TTTTTAGATG GTATAACGAG ACAAACTATT ATTGAAATTG CAAAAAATTT AGGATTAGAA GTAAAAGAAG AACGTCTAAA ATTAGAACAG ATAGAAGATT TTATCAGCTG TTTTGCTACA GGTACAGCAA TTGAAGTGCA GAATATTAAT TCTATAGATA TTGGTAATAA GAAAGTTATT TTTAATGATC ATAAAATTGC AGATGTTTTG AAAGAAGAGT ATGGTAAAAT TGTGAGGGGG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT