RibeA.10201.a

Methionyl-tRNA synthetase

CENTER ID: RibeA.10201.a
ORGANISM: Rickettsia bellii RML369-C
ASSOCIATED DISEASE: Typhus
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.10201.a.B1.GE35560 Full length( RibeA.10201.a ) 1 509
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.10201.a.B1.PW36165 Full length( RibeA.10201.a ) 1 509

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|336407.7.peg.272
RefSeq: YP_537408.1
UniProt: Q1RJZ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNNTYYITTP IYYVNDVPHI GHAYTSVASD VIARFMRLSG HEVMFLTGTD EHGQKVEKAA IDKNIDPQKF TDQTSQSFRH LMTAMHISND DFIRTTEERH KKAVAIFWQK LLDNGSIYEG FYEGWYAVRD EAFYDESELT ADKLAPTGAA VEWVKEPSYF FNLSKWQDKL LEFYEANPDF IRPISRRNEV ISFVKSGLKD LSVSRTTFNW GIKVPNDNKH VIYVWLDALA NYISALGYPD QNSNYGKFWP ANLQVVGKDI LRFHAVYWPA FLMAAEIPLP KTIMAHGWWT NEGQKISKSL GNTIDPIKLI EEFGVDQVRY FLMREITFGA DGNFARSNLI TRINSELSNK IGNLLQRTTS FVYKNNDGKV PAITQDAINK IYELPLLKTA INSAKQNILL MEKTEINKIL DNIINLAEEA NIYIDSEAPW NLKKTDPEKM LEVLYALLET LRYIAVMLQA FMPSSAGKML DQLGVNTEER LFKHLSLEFA LTSASDILEP VIVFPRFEE
NT Sequence
ATGAATAATA CCTACTATAT TACCACTCCC ATATATTATG TTAATGATGT CCCGCATATT GGGCATGCTT ATACCAGCGT TGCAAGTGAC GTGATTGCCC GCTTTATGCG TCTTAGCGGT CATGAAGTCA TGTTTTTAAC CGGCACTGAT GAACATGGGC AGAAAGTAGA AAAAGCTGCT ATTGATAAAA ATATTGATCC GCAAAAATTT ACTGATCAGA CTTCTCAAAG TTTTCGTCAT CTTATGACTG CCATGCATAT TTCAAATGAT GATTTTATCA GAACAACGGA AGAAAGGCAT AAAAAAGCAG TAGCTATATT TTGGCAAAAA TTACTAGATA ACGGTTCTAT CTATGAGGGT TTTTATGAGG GGTGGTATGC CGTTCGTGAT GAAGCTTTTT ATGATGAATC TGAGCTAACA GCGGACAAAT TAGCCCCAAC CGGTGCAGCG GTGGAATGGG TTAAAGAACC TAGCTATTTC TTTAATCTCT CAAAATGGCA AGATAAATTG CTTGAGTTTT ATGAAGCAAA CCCTGATTTT ATCAGACCTA TATCAAGACG TAACGAGGTC ATCAGCTTTG TTAAGTCAGG TCTAAAAGAT TTGTCCGTAT CACGCACCAC TTTTAACTGG GGTATCAAAG TCCCAAACGA TAATAAGCAC GTGATTTATG TGTGGCTTGA TGCACTTGCT AATTATATCT CAGCTCTTGG CTATCCTGAT CAAAATAGCA ATTACGGCAA GTTTTGGCCA GCCAATTTAC AGGTAGTCGG TAAAGATATT CTACGTTTCC ATGCCGTTTA TTGGCCAGCT TTCTTAATGG CTGCTGAAAT TCCGCTACCT AAGACAATTA TGGCACATGG CTGGTGGACT AATGAGGGGC AGAAAATTTC TAAATCACTT GGTAATACTA TTGATCCGAT TAAGCTGATA GAGGAGTTTG GTGTTGATCA GGTTAGATAT TTTTTAATGC GTGAGATAAC ATTTGGAGCG GATGGTAACT TTGCCCGCAG CAATCTAATT ACCCGTATTA ATAGCGAATT ATCTAATAAG ATCGGTAATT TATTGCAACG TACTACTTCT TTTGTTTATA AAAATAATGA TGGCAAAGTG CCAGCAATCA CGCAAGATGC AATTAATAAA ATATATGAAT TACCGCTTCT AAAAACAGCA ATTAATTCTG CCAAACAGAA TATTTTGTTA ATGGAAAAAA CCGAGATTAA CAAGATTCTT GATAATATTA TTAATTTAGC CGAAGAAGCA AATATTTATA TTGATAGCGA AGCTCCTTGG AATTTGAAAA AAACAGACCC TGAGAAAATG CTAGAGGTGT TATATGCTCT GCTAGAAACT TTGCGTTATA TCGCCGTAAT GCTTCAGGCT TTTATGCCAA GTTCTGCCGG TAAAATGCTT GATCAGCTAG GGGTAAACAC AGAAGAGCGT TTATTTAAGC ATTTATCGCT TGAGTTTGCT TTAACATCAG CTAGCGATAT TCTAGAACCG GTTATAGTAT TTCCAAGGTT TGAAGAG
Details for RibeA.10201.a.B1.GE35560
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 59kDa
OBSERVED MW: 59kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 89
Validated AA Sequence
MRLSGHEVMF LTGTDEHGQK VEKAAIDKNI DPQKFTDQTS QSFRHLMTAM HISNDDFIRT TEERHKKAVA IFWQKLLDNG SIYEGFYEGW YAVRDEAFYD ESELTADKLA PTGAAVEWVK EPSYFFNLSK WQDKLLEFYE ANPDFIRPIS RRNEVISFVK SGLKDLSVSR TTFNWGIKVP NDNKHVIYVW LDALANYISA LGYPDQNSNY GKFWPANLQV VGKDILRFHA VYWPAFLMAA EIPLPKTIMA HGWWTNEGQK ISKSLGNTID PIKLIEEFGV DQVRYFLMRE ITFGADGNFA RSNLITRINS ELSNKIGNLL QRTTSFVYKN NDGKVPAITQ DAINKIYELP LLKTAINSAK QNILLMEKTE INKILDNIIN LAEEANIYID SEAPWNLKKT DPEKMLEVLY ALLETLRYIA VMLQAFMPSS AGKMLDQLGV NTEERLFKHL SLEFALTSAS DILEP
Validated NT Sequence
accggttcta gaatatcgct agctgatgtt aaagcaaact caagcgataa atgcttaaat aaacgctctt ctgtgtttac ccctagctga tcaagcattt taccggcaga acttggcata aaagcctgaa gcattacggc gatataacgc aaagtttcta gcagagcata taacacctct agcattttct cagggtctgt ttttttcaaa ttccaaggag cttcgctatc aatataaata tttgcttctt cggctaaatt aataatatta tcaagaatct tgttaatctc ggttttttcc attaacaaaa tattctgttt ggcagaatta attgctgttt ttagaagcgg taattcatat attttattaa ttgcatcttg cgtgattgct ggcactttgc catcattatt tttataaaca aaagaagtag tacgttgcaa taaattaccg atcttattag ataattcgct attaatacgg gtaattagat tgctgcgggc aaagttacca tccgctccaa atgttatctc acgcattaaa aaatatctaa cctgatcaac accaaactcc tctatcagct taatcggatc aatagtatta ccaagtgatt tagaaatttt ctgcccctca ttagtccacc agccatgtgc cataattgtc ttaggtagcg gaatttcagc agccattaag aaagctggcc aataaacggc atggaaacgt agaatatctt taccgactac ctgtaaattg gctggccaaa acttgccgta attgctattt tgatcaggat agccaagagc tgagatataa ttagcaagtg catcaagcca cacataaatc acgtgcttat tatcgtttgg gactttgata ccccagttaa aagtggtgcg tgatacggac aaatctttta gacctgactt aacaaagctg atgacctcgt tacgtcttga tataggtctg ataaaatcag ggtttgcttc ataaaactca agcaatttat cttgccattt tgagagatta aagaaatagc taggttcttt aacccattcc accgctgcac cggttggggc taatttgtcc gctgttagct cagattcatc ataaaaagct tcatcacgaa cggcatacca cccctcataa aaaccctcat agatagaacc gttatctagt aatttttgcc aaaatatagc tactgctttt ttatgccttt cttccgttgt tctgataaaa tcatcatttg aaatatgcat ggcagtcata agatgacgaa aactttgaga agtctgatca gtaaattttt gcggatcaat atttttatca atagcagctt tttctacttt ctgcccatgt tcatcagtgc cggttaaaaa catgacttca tgaccgctaa gacgcataaa gcgggcaatc acgtcacttg caacgctggt ataagcatgc ccaatatgcg ggacatcatt aacataatat atgggagtgg taatatagta ggtatt
Expected Protein Sequence
MAHHHHHHMN NTYYITTPIY YVNDVPHIGH AYTSVASDVI ARFMRLSGHE VMFLTGTDEH GQKVEKAAID KNIDPQKFTD QTSQSFRHLM TAMHISNDDF IRTTEERHKK AVAIFWQKLL DNGSIYEGFY EGWYAVRDEA FYDESELTAD KLAPTGAAVE WVKEPSYFFN LSKWQDKLLE FYEANPDFIR PISRRNEVIS FVKSGLKDLS VSRTTFNWGI KVPNDNKHVI YVWLDALANY ISALGYPDQN SNYGKFWPAN LQVVGKDILR FHAVYWPAFL MAAEIPLPKT IMAHGWWTNE GQKISKSLGN TIDPIKLIEE FGVDQVRYFL MREITFGADG NFARSNLITR INSELSNKIG NLLQRTTSFV YKNNDGKVPA ITQDAINKIY ELPLLKTAIN SAKQNILLME KTEINKILDN IINLAEEANI YIDSEAPWNL KKTDPEKMLE VLYALLETLR YIAVMLQAFM PSSAGKMLDQ LGVNTEERLF KHLSLEFALT SASDILEPVI VFPRFEE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata atacctacta tattaccact cccatatatt atgttaatga tgtcccgcat attgggcatg cttataccag cgttgcaagt gacgtgattg cccgctttat gcgtcttagc ggtcatgaag tcatgttttt aaccggcact gatgaacatg ggcagaaagt agaaaaagct gctattgata aaaatattga tccgcaaaaa tttactgatc agacttctca aagttttcgt catcttatga ctgccatgca tatttcaaat gatgatttta tcagaacaac ggaagaaagg cataaaaaag cagtagctat attttggcaa aaattactag ataacggttc tatctatgag ggtttttatg aggggtggta tgccgttcgt gatgaagctt tttatgatga atctgagcta acagcggaca aattagcccc aaccggtgca gcggtggaat gggttaaaga acctagctat ttctttaatc tctcaaaatg gcaagataaa ttgcttgagt tttatgaagc aaaccctgat tttatcagac ctatatcaag acgtaacgag gtcatcagct ttgttaagtc aggtctaaaa gatttgtccg tatcacgcac cacttttaac tggggtatca aagtcccaaa cgataataag cacgtgattt atgtgtggct tgatgcactt gctaattata tctcagctct tggctatcct gatcaaaata gcaattacgg caagttttgg ccagccaatt tacaggtagt cggtaaagat attctacgtt tccatgccgt ttattggcca gctttcttaa tggctgctga aattccgcta cctaagacaa ttatggcaca tggctggtgg actaatgagg ggcagaaaat ttctaaatca cttggtaata ctattgatcc gattaagctg atagaggagt ttggtgttga tcaggttaga tattttttaa tgcgtgagat aacatttgga gcggatggta actttgcccg cagcaatcta attacccgta ttaatagcga attatctaat aagatcggta atttattgca acgtactact tcttttgttt ataaaaataa tgatggcaaa gtgccagcaa tcacgcaaga tgcaattaat aaaatatatg aattaccgct tctaaaaaca gcaattaatt ctgccaaaca gaatattttg ttaatggaaa aaaccgagat taacaagatt cttgataata ttattaattt agccgaagaa gcaaatattt atattgatag cgaagctcct tggaatttga aaaaaacaga ccctgagaaa atgctagagg tgttatatgc tctgctagaa actttgcgtt atatcgccgt aatgcttcag gcttttatgc caagttctgc cggtaaaatg cttgatcagc taggggtaaa cacagaagag cgtttattta agcatttatc gcttgagttt gctttaacat cagctagcga tattctagaa ccggttatag tatttccaag gtttgaagag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for RibeA.10201.a.B1.PW36165
PURIFICATION DATe: 7/20/2012
CONCENTRATION: 24.18mg/ml
OBSERVED MW: 56kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 13
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 89
Protocol Notes
notes unavailable
Validated AA Sequence
MRLSGHEVMF LTGTDEHGQK VEKAAIDKNI DPQKFTDQTS QSFRHLMTAM HISNDDFIRT TEERHKKAVA IFWQKLLDNG SIYEGFYEGW YAVRDEAFYD ESELTADKLA PTGAAVEWVK EPSYFFNLSK WQDKLLEFYE ANPDFIRPIS RRNEVISFVK SGLKDLSVSR TTFNWGIKVP NDNKHVIYVW LDALANYISA LGYPDQNSNY GKFWPANLQV VGKDILRFHA VYWPAFLMAA EIPLPKTIMA HGWWTNEGQK ISKSLGNTID PIKLIEEFGV DQVRYFLMRE ITFGADGNFA RSNLITRINS ELSNKIGNLL QRTTSFVYKN NDGKVPAITQ DAINKIYELP LLKTAINSAK QNILLMEKTE INKILDNIIN LAEEANIYID SEAPWNLKKT DPEKMLEVLY ALLETLRYIA VMLQAFMPSS AGKMLDQLGV NTEERLFKHL SLEFALTSAS DILEP
Validated NT Sequence
accggttcta gaatatcgct agctgatgtt aaagcaaact caagcgataa atgcttaaat aaacgctctt ctgtgtttac ccctagctga tcaagcattt taccggcaga acttggcata aaagcctgaa gcattacggc gatataacgc aaagtttcta gcagagcata taacacctct agcattttct cagggtctgt ttttttcaaa ttccaaggag cttcgctatc aatataaata tttgcttctt cggctaaatt aataatatta tcaagaatct tgttaatctc ggttttttcc attaacaaaa tattctgttt ggcagaatta attgctgttt ttagaagcgg taattcatat attttattaa ttgcatcttg cgtgattgct ggcactttgc catcattatt tttataaaca aaagaagtag tacgttgcaa taaattaccg atcttattag ataattcgct attaatacgg gtaattagat tgctgcgggc aaagttacca tccgctccaa atgttatctc acgcattaaa aaatatctaa cctgatcaac accaaactcc tctatcagct taatcggatc aatagtatta ccaagtgatt tagaaatttt ctgcccctca ttagtccacc agccatgtgc cataattgtc ttaggtagcg gaatttcagc agccattaag aaagctggcc aataaacggc atggaaacgt agaatatctt taccgactac ctgtaaattg gctggccaaa acttgccgta attgctattt tgatcaggat agccaagagc tgagatataa ttagcaagtg catcaagcca cacataaatc acgtgcttat tatcgtttgg gactttgata ccccagttaa aagtggtgcg tgatacggac aaatctttta gacctgactt aacaaagctg atgacctcgt tacgtcttga tataggtctg ataaaatcag ggtttgcttc ataaaactca agcaatttat cttgccattt tgagagatta aagaaatagc taggttcttt aacccattcc accgctgcac cggttggggc taatttgtcc gctgttagct cagattcatc ataaaaagct tcatcacgaa cggcatacca cccctcataa aaaccctcat agatagaacc gttatctagt aatttttgcc aaaatatagc tactgctttt ttatgccttt cttccgttgt tctgataaaa tcatcatttg aaatatgcat ggcagtcata agatgacgaa aactttgaga agtctgatca gtaaattttt gcggatcaat atttttatca atagcagctt tttctacttt ctgcccatgt tcatcagtgc cggttaaaaa catgacttca tgaccgctaa gacgcataaa gcgggcaatc acgtcacttg caacgctggt ataagcatgc ccaatatgcg ggacatcatt aacataatat atgggagtgg taatatagta ggtatt
Expressed Protein Sequence
MAHHHHHHMN NTYYITTPIY YVNDVPHIGH AYTSVASDVI ARFMRLSGHE VMFLTGTDEH GQKVEKAAID KNIDPQKFTD QTSQSFRHLM TAMHISNDDF IRTTEERHKK AVAIFWQKLL DNGSIYEGFY EGWYAVRDEA FYDESELTAD KLAPTGAAVE WVKEPSYFFN LSKWQDKLLE FYEANPDFIR PISRRNEVIS FVKSGLKDLS VSRTTFNWGI KVPNDNKHVI YVWLDALANY ISALGYPDQN SNYGKFWPAN LQVVGKDILR FHAVYWPAFL MAAEIPLPKT IMAHGWWTNE GQKISKSLGN TIDPIKLIEE FGVDQVRYFL MREITFGADG NFARSNLITR INSELSNKIG NLLQRTTSFV YKNNDGKVPA ITQDAINKIY ELPLLKTAIN SAKQNILLME KTEINKILDN IINLAEEANI YIDSEAPWNL KKTDPEKMLE VLYALLETLR YIAVMLQAFM PSSAGKMLDQ LGVNTEERLF KHLSLEFALT SASDILEPVI VFPRFEE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATA ATACCTACTA TATTACCACT CCCATATATT ATGTTAATGA TGTCCCGCAT ATTGGGCATG CTTATACCAG CGTTGCAAGT GACGTGATTG CCCGCTTTAT GCGTCTTAGC GGTCATGAAG TCATGTTTTT AACCGGCACT GATGAACATG GGCAGAAAGT AGAAAAAGCT GCTATTGATA AAAATATTGA TCCGCAAAAA TTTACTGATC AGACTTCTCA AAGTTTTCGT CATCTTATGA CTGCCATGCA TATTTCAAAT GATGATTTTA TCAGAACAAC GGAAGAAAGG CATAAAAAAG CAGTAGCTAT ATTTTGGCAA AAATTACTAG ATAACGGTTC TATCTATGAG GGTTTTTATG AGGGGTGGTA TGCCGTTCGT GATGAAGCTT TTTATGATGA ATCTGAGCTA ACAGCGGACA AATTAGCCCC AACCGGTGCA GCGGTGGAAT GGGTTAAAGA ACCTAGCTAT TTCTTTAATC TCTCAAAATG GCAAGATAAA TTGCTTGAGT TTTATGAAGC AAACCCTGAT TTTATCAGAC CTATATCAAG ACGTAACGAG GTCATCAGCT TTGTTAAGTC AGGTCTAAAA GATTTGTCCG TATCACGCAC CACTTTTAAC TGGGGTATCA AAGTCCCAAA CGATAATAAG CACGTGATTT ATGTGTGGCT TGATGCACTT GCTAATTATA TCTCAGCTCT TGGCTATCCT GATCAAAATA GCAATTACGG CAAGTTTTGG CCAGCCAATT TACAGGTAGT CGGTAAAGAT ATTCTACGTT TCCATGCCGT TTATTGGCCA GCTTTCTTAA TGGCTGCTGA AATTCCGCTA CCTAAGACAA TTATGGCACA TGGCTGGTGG ACTAATGAGG GGCAGAAAAT TTCTAAATCA CTTGGTAATA CTATTGATCC GATTAAGCTG ATAGAGGAGT TTGGTGTTGA TCAGGTTAGA TATTTTTTAA TGCGTGAGAT AACATTTGGA GCGGATGGTA ACTTTGCCCG CAGCAATCTA ATTACCCGTA TTAATAGCGA ATTATCTAAT AAGATCGGTA ATTTATTGCA ACGTACTACT TCTTTTGTTT ATAAAAATAA TGATGGCAAA GTGCCAGCAA TCACGCAAGA TGCAATTAAT AAAATATATG AATTACCGCT TCTAAAAACA GCAATTAATT CTGCCAAACA GAATATTTTG TTAATGGAAA AAACCGAGAT TAACAAGATT CTTGATAATA TTATTAATTT AGCCGAAGAA GCAAATATTT ATATTGATAG CGAAGCTCCT TGGAATTTGA AAAAAACAGA CCCTGAGAAA ATGCTAGAGG TGTTATATGC TCTGCTAGAA ACTTTGCGTT ATATCGCCGT AATGCTTCAG GCTTTTATGC CAAGTTCTGC CGGTAAAATG CTTGATCAGC TAGGGGTAAA CACAGAAGAG CGTTTATTTA AGCATTTATC GCTTGAGTTT GCTTTAACAT CAGCTAGCGA TATTCTAGAA CCGGTTATAG TATTTCCAAG GTTTGAAGAG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT