SSGCID: Target Page

RibeA.10100.a

Periplasmic serine protease

CENTER ID:	RibeA.10100.a
ORGANISM:	Rickettsia bellii RML369-C
ASSOCIATED DISEASE:	Typhus
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RibeA.10100.a.B2.GE35565	Mature protein( RibeA.10100.a )		20	512

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RibeA.10100.a.B2.PW36170	Mature protein( RibeA.10100.a )		20	512

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|336407.7.peg.1321
RefSeq:	YP_538340.1
UniProt:	Q1RHB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNLKIFFIII ILIFNNNILA KENNKSSKAI DLEEESEFTE INSIPLKVNN ASRYSFADIV EPLIPAVVNI STIEYVNSKS EAAEKDPLQE KKPLDFINDF LERLNIPLNL EEVDPTPKAM PLGSGFIIEP NGLIVTNYHV IANVSKINVK LADNTELPAK LIGSDTKTDL ALLKIDVEEP LPFVEFGDSN DARVGDWVIA IGNPFGNLGG TVTAGIISSK GRDIDIDTNN IVDNFIQTDA AINNGNSGGP MFNLDQKVIG VNTAIFSPLG TNIGIGFAIP SNTAKPIIER LKKDGKINRG RLGVTIQDLT EEISEGLGLK DTNGVLVAKI QKDDPGDKAG IKTGDIILEF AGQTVKNTKR LRVIVADIPV DQEVKVKVLR DGEAIELPIK MTSDNEEVKQ EAAEENIEKV TKKEDNGTSI TKSNITFSNL TEELKQKFAI SVDKVGLVIT NVDEEDSSFR VGDLVSNVNQ ESISDINKLE ELYENAKKSE KQNILLLIER GDSSMFVPLS VM

NT Sequence

ATGAATCTGA AAATATTTTT TATTATAATA ATTTTAATAT TTAATAACAA TATTTTAGCG AAAGAAAATA ATAAATCTTC AAAAGCAATA GATTTGGAGG AGGAAAGTGA ATTTACCGAA ATAAATTCCA TACCTCTAAA AGTAAATAAT GCATCACGTT ATAGTTTTGC TGATATAGTT GAGCCTTTAA TCCCTGCTGT TGTTAATATT TCTACTATAG AATATGTTAA TAGTAAGTCA GAAGCTGCAG AAAAAGATCC TTTACAAGAA AAAAAACCTC TGGATTTCAT TAATGATTTT CTAGAACGTC TTAACATACC TCTCAACCTT GAAGAAGTTG ATCCTACTCC AAAAGCGATG CCGCTCGGTT CTGGGTTTAT TATTGAGCCT AATGGTTTGA TAGTTACAAA TTATCACGTG ATTGCTAATG TTAGTAAAAT TAATGTAAAA TTAGCCGATA ACACTGAATT ACCAGCTAAA TTAATAGGTA GTGATACTAA AACTGATTTA GCTCTTTTAA AAATAGATGT TGAAGAACCG CTGCCTTTCG TAGAATTTGG TGACTCAAAT GATGCAAGAG TAGGGGACTG GGTTATTGCA ATCGGTAATC CTTTTGGTAA TCTTGGTGGT ACTGTTACTG CCGGTATTAT TTCTTCAAAA GGACGAGATA TTGATATTGA CACTAACAAT ATAGTCGATA ATTTTATCCA AACAGATGCG GCAATTAATA ACGGCAATTC TGGTGGTCCT ATGTTTAATT TGGACCAAAA AGTAATTGGT GTAAATACGG CAATTTTCTC ACCGCTTGGC ACTAATATAG GTATTGGTTT TGCTATCCCT TCAAATACTG CAAAGCCTAT AATTGAGCGT CTTAAAAAAG ATGGTAAAAT AAATAGAGGG CGTCTTGGTG TAACAATACA AGATTTGACT GAAGAAATAT CAGAAGGATT AGGGCTAAAA GACACTAATG GTGTGTTAGT TGCTAAAATA CAAAAAGATG ATCCAGGTGA TAAAGCAGGC ATTAAAACAG GCGATATAAT ATTAGAGTTT GCAGGGCAGA CAGTTAAAAA CACTAAAAGA TTACGTGTAA TCGTGGCTGA TATTCCTGTT GATCAAGAAG TGAAAGTAAA AGTATTACGA GACGGAGAAG CAATAGAACT ACCAATAAAA ATGACATCAG ATAATGAGGA AGTTAAACAA GAAGCTGCGG AAGAAAATAT AGAAAAAGTT ACTAAGAAAG AAGATAATGG CACGTCTATT ACAAAGAGTA ATATCACTTT TAGTAATTTG ACTGAGGAAT TAAAGCAAAA ATTTGCTATA TCTGTTGATA AAGTAGGATT AGTTATTACA AATGTTGATG AAGAAGATAG TAGCTTTAGA GTTGGTGATT TAGTAAGTAA TGTTAATCAA GAAAGCATAA GCGATATAAA TAAACTAGAA GAATTATACG AAAATGCTAA AAAATCAGAA AAGCAAAATA TTTTGCTTTT GATTGAGAGA GGTGATAGTA GTATGTTCGT TCCATTATCT GTAATG

Details for RibeA.10100.a.B2.GE35565

HARVESTED ON:	6/12/2012
SEQUENCED ON:	6/12/2012
EXPECTED MW:	54kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	76

Validated AA Sequence

MPLGSGFIIE PNGLIVTNYH VIANVSKINV KLADNTELPA KLIGSDTKTD LALLKIDVEE PLPFVEFGDS NDARVGDWVI AIGNPFGNLG GTVTAGIISS KGRDIDIDTN NIVDNFIQTD AAINNGNSGG PMFNLDQKVI GVNTAIFSPL GTNIGIGXAI PSNTAKPIIE RLKKDGKINR GRLGVTIQDL TEEISEGLGL KDTNGVLVAK IQKDDPGDKA GIKTGDIILE FAGQTVKNTK RLRVIVADIP VDQEVKVKVL RDGEAIELPI KMTSDNEEVK QEAAEENIEK VTKKEDNGTS ITKSNITFSN LTEELKQKFA ISVDKVGLVI TNVDEEDSSF RVGDLVSNVN QESISDINKL EELYENAKKS EKQNILLLIE RGDS

Validated NT Sequence

ctactatcac ctctctcaat caaaagcaaa atattttgct tttctgattt tttagcattt tcgtataatt cttctagttt atttatatcg cttatgcttt cttgattaac attacttact aaatcaccaa ctctaaagct actatcttct tcatcaacat ttgtaataac taatcctact ttatcaacag atatagcaaa tttttgcttt aattcctcag tcaaattact aaaagtgata ttactctttg taatagacgt gccattatct tctttcttag taactttttc tatattttct tccgcagctt cttgtttaac ttcctcatta tctgatgtca tttttattgg tagttctatt gcttctccgt ctcgtaatac ttttactttc acttcttgat caacaggaat atcagccacg attacncgta atcttttagt gtttttaact gtctgccctg caaactctaa tattatatcg cctgttttaa tgcctgcttt atcacctgga tcatcttttt gtattttagc aactaacaca ccattagtgt cttttagccc taatccttct gatatttctt cagtcaaatc ttgtattgtt acaccaagac gccctctatt tattttacca tcttttttaa gacgctcaat tataggcttt gcagtatttg aagggatagc anaaccaata cctatattag tgccaagcgg tgagaaaatt gccgtattta caccaattac tttttggtcc aaattaaaca taggaccacc agaattgccg ttattaattg ccgcatctgt ttggataaaa ttatcgacta tattgttagt gtcaatatca atatctcgtc cttttgaaga aataataccg gcagtaacag taccaccaag attaccaaaa ggattaccga ttgcaataac ccagtcccct actcttgcat catttgagtc accaaattct acgaaaggca gcggttcttc aacatctatt tttaaaagag ctaaatcagt tttagtatca ctacctatta atttagctgg taattcagtg ttatcggcta attttacatt aattttacta acattagcaa tcacgtgata atttgtaact atcaaaccat taggctcaat aataaaccca gaaccgagcg gcatcgcttt tggagtagga tcaacttctt caaggttgag aggtatgtta agacgttcta gaaaatcatt aatgaaatcc agaggttttt tttcttgtaa aggatctttt tctgcagctt ctgacttact attaacatat tctatagtag aaatattaac aacagcaggg attaaaggct caactatatc agcaaaacta taacgtgatg cattatttac ttttagaggt atggaattta tttcggtaaa ttcactttcc tcctccaaat ctattgcttt tgaagattta ttattttctt tcgcca

Expected Protein Sequence

MAHHHHHHMA KENNKSSKAI DLEEESEFTE INSIPLKVNN ASRYSFADIV EPLIPAVVNI STIEYVNSKS EAAEKDPLQE KKPLDFINDF LERLNIPLNL EEVDPTPKAM PLGSGFIIEP NGLIVTNYHV IANVSKINVK LADNTELPAK LIGSDTKTDL ALLKIDVEEP LPFVEFGDSN DARVGDWVIA IGNPFGNLGG TVTAGIISSK GRDIDIDTNN IVDNFIQTDA AINNGNSGGP MFNLDQKVIG VNTAIFSPLG TNIGIGFAIP SNTAKPIIER LKKDGKINRG RLGVTIQDLT EEISEGLGLK DTNGVLVAKI QKDDPGDKAG IKTGDIILEF AGQTVKNTKR LRVIVADIPV DQEVKVKVLR DGEAIELPIK MTSDNEEVKQ EAAEENIEKV TKKEDNGTSI TKSNITFSNL TEELKQKFAI SVDKVGLVIT NVDEEDSSFR VGDLVSNVNQ ESISDINKLE ELYENAKKSE KQNILLLIER GDSSMFVPLS VM

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga aagaaaataa taaatcttca aaagcaatag atttggagga ggaaagtgaa tttaccgaaa taaattccat acctctaaaa gtaaataatg catcacgtta tagttttgct gatatagttg agcctttaat ccctgctgtt gttaatattt ctactataga atatgttaat agtaagtcag aagctgcaga aaaagatcct ttacaagaaa aaaaacctct ggatttcatt aatgattttc tagaacgtct taacatacct ctcaaccttg aagaagttga tcctactcca aaagcgatgc cgctcggttc tgggtttatt attgagccta atggtttgat agttacaaat tatcacgtga ttgctaatgt tagtaaaatt aatgtaaaat tagccgataa cactgaatta ccagctaaat taataggtag tgatactaaa actgatttag ctcttttaaa aatagatgtt gaagaaccgc tgcctttcgt agaatttggt gactcaaatg atgcaagagt aggggactgg gttattgcaa tcggtaatcc ttttggtaat cttggtggta ctgttactgc cggtattatt tcttcaaaag gacgagatat tgatattgac actaacaata tagtcgataa ttttatccaa acagatgcgg caattaataa cggcaattct ggtggtccta tgtttaattt ggaccaaaaa gtaattggtg taaatacggc aattttctca ccgcttggca ctaatatagg tattggtttt gctatccctt caaatactgc aaagcctata attgagcgtc ttaaaaaaga tggtaaaata aatagagggc gtcttggtgt aacaatacaa gatttgactg aagaaatatc agaaggatta gggctaaaag acactaatgg tgtgttagtt gctaaaatac aaaaagatga tccaggtgat aaagcaggca ttaaaacagg cgatataata ttagagtttg cagggcagac agttaaaaac actaaaagat tacgtgtaat cgtggctgat attcctgttg atcaagaagt gaaagtaaaa gtattacgag acggagaagc aatagaacta ccaataaaaa tgacatcaga taatgaggaa gttaaacaag aagctgcgga agaaaatata gaaaaagtta ctaagaaaga agataatggc acgtctatta caaagagtaa tatcactttt agtaatttga ctgaggaatt aaagcaaaaa tttgctatat ctgttgataa agtaggatta gttattacaa atgttgatga agaagatagt agctttagag ttggtgattt agtaagtaat gttaatcaag aaagcataag cgatataaat aaactagaag aattatacga aaatgctaaa aaatcagaaa agcaaaatat tttgcttttg attgagagag gtgatagtag tatgttcgtt ccattatctg taatgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for RibeA.10100.a.B2.PW36170

PURIFICATION DATe:	7/23/2012
CONCENTRATION:	29.27mg/ml
OBSERVED MW:	56kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	76

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGA AAGAAAATAA TAAATCTTCA AAAGCAATAG ATTTGGAGGA GGAAAGTGAA TTTACCGAAA TAAATTCCAT ACCTCTAAAA GTAAATAATG CATCACGTTA TAGTTTTGCT GATATAGTTG AGCCTTTAAT CCCTGCTGTT GTTAATATTT CTACTATAGA ATATGTTAAT AGTAAGTCAG AAGCTGCAGA AAAAGATCCT TTACAAGAAA AAAAACCTCT GGATTTCATT AATGATTTTC TAGAACGTCT TAACATACCT CTCAACCTTG AAGAAGTTGA TCCTACTCCA AAAGCGATGC CGCTCGGTTC TGGGTTTATT ATTGAGCCTA ATGGTTTGAT AGTTACAAAT TATCACGTGA TTGCTAATGT TAGTAAAATT AATGTAAAAT TAGCCGATAA CACTGAATTA CCAGCTAAAT TAATAGGTAG TGATACTAAA ACTGATTTAG CTCTTTTAAA AATAGATGTT GAAGAACCGC TGCCTTTCGT AGAATTTGGT GACTCAAATG ATGCAAGAGT AGGGGACTGG GTTATTGCAA TCGGTAATCC TTTTGGTAAT CTTGGTGGTA CTGTTACTGC CGGTATTATT TCTTCAAAAG GACGAGATAT TGATATTGAC ACTAACAATA TAGTCGATAA TTTTATCCAA ACAGATGCGG CAATTAATAA CGGCAATTCT GGTGGTCCTA TGTTTAATTT GGACCAAAAA GTAATTGGTG TAAATACGGC AATTTTCTCA CCGCTTGGCA CTAATATAGG TATTGGTTTT GCTATCCCTT CAAATACTGC AAAGCCTATA ATTGAGCGTC TTAAAAAAGA TGGTAAAATA AATAGAGGGC GTCTTGGTGT AACAATACAA GATTTGACTG AAGAAATATC AGAAGGATTA GGGCTAAAAG ACACTAATGG TGTGTTAGTT GCTAAAATAC AAAAAGATGA TCCAGGTGAT AAAGCAGGCA TTAAAACAGG CGATATAATA TTAGAGTTTG CAGGGCAGAC AGTTAAAAAC ACTAAAAGAT TACGTGTAAT CGTGGCTGAT ATTCCTGTTG ATCAAGAAGT GAAAGTAAAA GTATTACGAG ACGGAGAAGC AATAGAACTA CCAATAAAAA TGACATCAGA TAATGAGGAA GTTAAACAAG AAGCTGCGGA AGAAAATATA GAAAAAGTTA CTAAGAAAGA AGATAATGGC ACGTCTATTA CAAAGAGTAA TATCACTTTT AGTAATTTGA CTGAGGAATT AAAGCAAAAA TTTGCTATAT CTGTTGATAA AGTAGGATTA GTTATTACAA ATGTTGATGA AGAAGATAGT AGCTTTAGAG TTGGTGATTT AGTAAGTAAT GTTAATCAAG AAAGCATAAG CGATATAAAT AAACTAGAAG AATTATACGA AAATGCTAAA AAATCAGAAA AGCAAAATAT TTTGCTTTTG ATTGAGAGAG GTGATAGTAG TATGTTCGTT CCATTATCTG TAATGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT