SSGCID: Target Page

RibeA.10014.f

O-antigen export system ATP-binding protein RfbE

CENTER ID:	RibeA.10014.f
ORGANISM:	Rickettsia bellii RML369-C
ASSOCIATED DISEASE:	Typhus
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RibeA.10014.f.B1.GE35567	Full length( RibeA.10014.f )		1	248

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|336407.7.peg.3
RefSeq:	YP_537173.1
UniProt:	Q1RKN0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSFNHSKVFI EIKNAYVEGI DTHKRAQGLK HFFLNRGTSF SPNIPILKKI NFSCYEGEKV AFVGSNGSGK SSLLKMIAGI YPPIKGTINV CGEIAPIIDM GVGFEQEQTG RDNIKMLMLY NNMLDKYNKE TEQEIIDFSE LGKKIDLPIK NYSSGMLARL AFSTSIFQNP QILLLDEVFA TGDNYFVEKS LNLMKNKFRN TPISIIVTHQ ENIVKENCTR CILLRDGNIV GDGKPSEIFK IYTRRKRK

NT Sequence

ATGTCTTTTA ATCATTCTAA AGTATTTATA GAGATAAAAA ACGCTTACGT TGAAGGTATA GATACCCACA AAAGAGCACA AGGATTAAAG CATTTCTTTC TTAACAGAGG GACTTCTTTT TCGCCAAATA TACCTATATT AAAAAAAATT AACTTTTCTT GTTATGAAGG GGAAAAAGTA GCTTTTGTAG GTAGTAATGG TTCAGGAAAA AGTTCACTTT TAAAAATGAT AGCAGGAATT TATCCGCCAA TAAAAGGGAC AATAAACGTT TGTGGCGAGA TTGCACCAAT AATAGATATG GGTGTTGGTT TTGAACAAGA ACAAACAGGT CGCGATAACA TCAAAATGTT GATGCTTTAT AACAATATGT TAGACAAGTA TAATAAAGAA ACAGAACAGG AAATTATAGA TTTTTCGGAA TTAGGTAAAA AAATTGATTT GCCTATAAAA AATTATAGTT CAGGAATGCT AGCACGACTT GCCTTTTCTA CATCAATATT TCAAAACCCT CAGATACTTT TACTTGATGA AGTTTTTGCT ACAGGAGATA ATTATTTTGT AGAAAAATCT TTAAATTTAA TGAAAAATAA GTTTAGAAAC ACACCTATTT CAATAATAGT AACTCATCAA GAAAATATAG TAAAAGAAAA TTGTACTAGA TGTATTTTAT TGAGAGATGG TAATATTGTT GGAGATGGTA AACCTTCAGA AATATTCAAA ATTTATACGA GAAGAAAAAG AAAA

Details for RibeA.10014.f.B1.GE35567

HARVESTED ON:	6/12/2012
SEQUENCED ON:	6/14/2012
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS FNHSKVFIEI KNAYVEGIDT HKRAQGLKHF FLNRGTSFSP NIPILKKINF SCYEGEKVAF VGSNGSGKSS LLKMIAGIYP PIKGTINVCG EIAPIIDMGV GFEQEQTGRD NIKMLMLYNN MLDKYNKETE QEIIDFSELG KKIDLPIKNY SSGMLARLAF STSIFQNPQI LLLDEVFATG DNYFVEKSLN LMKNKFRNTP ISIIVTHQEN IVKENCTRCI LLRDGNIVGD GKPSEIFKIY TRRKRK

Validated NT Sequence

atggctcacc accaccacca ccatatgtct tttaatcatt ctaaagtatt tatagagata aaaaacgctt acgttgaagg tatagatacc cacaaaagag cacaaggatt aaagcatttc tttcttaaca gagggacttc tttttcgcca aatataccta tattaaaaaa aattaacttt tcttgttatg aaggggaaaa agtagctttt gtaggtagta atggttcagg aaaaagttca cttttaaaaa tgatagcagg aatttatccg ccaataaaag ggacaataaa cgtttgtggc gagattgcac caataataga tatgggtgtt ggttttgaac aagaacaaac aggtcgcgat aacatcaaaa tgttgatgct ttataacaat atgttagaca agtataataa agaaacagaa caggaaatta tagatttttc ggaattaggt aaaaaaattg atttgcctat aaaaaattat agttcaggaa tgctagcacg acttgccttt tctacatcaa tatttcaaaa ccctcagata cttttacttg atgaagtttt tgctacagga gataattatt ttgtagaaaa atctttaaat ttaatgaaaa ataagtttag aaacacacct atttcaataa tagtaactca tcaagaaaat atagtaaaag aaaattgtac tagatgtatt ttattgagag atggtaatat tgttggagat ggtaaacctt cagaaatatt caaaatttat acnagaagaa aaagaaaata agtgagtaag at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctt ttaatcattc taaagtattt atagagataa aaaacgctta cgttgaaggt atagataccc acaaaagagc acaaggatta aagcatttct ttcttaacag agggacttct ttttcgccaa atatacctat attaaaaaaa attaactttt cttgttatga aggggaaaaa gtagcttttg taggtagtaa tggttcagga aaaagttcac ttttaaaaat gatagcagga atttatccgc caataaaagg gacaataaac gtttgtggcg agattgcacc aataatagat atgggtgttg gttttgaaca agaacaaaca ggtcgcgata acatcaaaat gttgatgctt tataacaata tgttagacaa gtataataaa gaaacagaac aggaaattat agatttttcg gaattaggta aaaaaattga tttgcctata aaaaattata gttcaggaat gctagcacga cttgcctttt ctacatcaat atttcaaaac cctcagatac ttttacttga tgaagttttt gctacaggag ataattattt tgtagaaaaa tctttaaatt taatgaaaaa taagtttaga aacacaccta tttcaataat agtaactcat caagaaaata tagtaaaaga aaattgtact agatgtattt tattgagaga tggtaatatt gttggagatg gtaaaccttc agaaatattc aaaatttata cgagaagaaa aagaaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at