RibeA.01656.a

Outer membrane protein Sca14

CENTER ID: RibeA.01656.a
ORGANISM: Rickettsia bellii RML369-C
ASSOCIATED DISEASE: Typhus
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.01656.a.B1.GE35547 Full length( RibeA.01656.a ) 1 366
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|336407.7.peg.436
RefSeq: YP_537554.1
UniProt: Q1RJJ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQHNFDANN SLNENIDNLA KNEVADNHNL TDESKINSTT EKDFENSENN LVHYANNSLT STKDSTTTEF APRRVVNNTD ILISNIDNRL NSLTRSFAAL AAGDEEQIYG FYINPFIGKA TQKETKEISR YHSTSKGTII GHDAHLNDSL QLGANFTRVE TKFNFPRYKT TVRSNYYSLY GLYTPSNQNI FTSIIGIYGI SDIINFNQIK NTHKAYTSTI QVMLGSNYKL FSTTHIKPML GLKYSKIREA SYLEDNLSIA SVKERTIEGI LGVKLANSNT LKSVIITPEI YAFAHKNIKS KIPSAQTNII DNRILPIYIR KLPLSFSYGA KLSLKTKSIE IGINYNGTKA NKFISHLGSI NFKINL
NT Sequence
TTGACCCAGC ATAATTTTGA TGCAAATAAT AGTTTAAACG AAAATATAGA TAATTTAGCT AAAAATGAAG TAGCTGATAA TCATAATCTA ACTGATGAAA GCAAAATAAA TAGTACTACT GAAAAAGACT TTGAAAATTC TGAAAATAAT TTAGTACATT ATGCTAACAA TAGCTTAACA TCAACGAAAG ATTCTACAAC CACAGAATTT GCACCACGTA GAGTGGTTAA TAATACCGAT ATTTTAATAT CTAATATTGA TAATAGATTA AATTCATTAA CAAGAAGTTT TGCAGCATTA GCCGCAGGAG ATGAAGAACA AATTTATGGA TTTTATATTA ATCCATTTAT AGGCAAAGCA ACTCAAAAAG AAACAAAAGA AATAAGTAGA TATCATAGTA CAAGTAAAGG CACAATAATA GGGCATGATG CACACTTAAA CGATTCTTTG CAACTTGGAG CAAATTTTAC TAGAGTAGAA ACAAAATTTA ATTTTCCAAG ATATAAAACT ACAGTTAGAA GTAATTACTA CTCATTGTAT GGACTATATA CACCTTCAAA CCAAAACATC TTTACAAGTA TAATTGGAAT ATATGGAATT AGTGATATAA TTAACTTTAA TCAAATAAAA AATACTCACA AAGCCTATAC TAGTACTATA CAAGTAATGC TTGGCTCTAA CTATAAGTTA TTTAGTACTA CGCATATTAA GCCTATGTTA GGACTTAAAT ATAGCAAAAT TAGAGAAGCA TCATATTTAG AAGATAATTT ATCAATAGCA AGTGTTAAAG AGCGGACGAT TGAAGGTATC TTAGGGGTAA AATTAGCAAA TTCAAATACA CTAAAATCAG TAATTATTAC TCCTGAAATA TATGCTTTTG CCCATAAAAA TATTAAGTCA AAAATACCGA GTGCTCAAAC AAATATTATA GATAATAGAA TATTACCTAT ATATATCAGG AAATTACCTT TAAGTTTTAG TTATGGGGCA AAATTAAGTC TTAAAACAAA ATCAATTGAG ATAGGAATAA ATTATAATGG AACTAAAGCA AATAAATTTA TATCGCATTT AGGAAGTATA AACTTCAAAA TTAATTTA
Details for RibeA.01656.a.B1.GE35547
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML TQHNFDANNS LNENIDNLAK NEVADNHNLT DESKINSTTE KDFENSENNL VHYANNSLTS TKDSTTTEFA PRRVVNNTDI LISNIDNRLN SLTRSFAALA AGDEEQIYGF YINPFIGKAT QKETKEISRY HSTSKGTIIG HDAHLNDSLQ LGANFTRVET KFNFPRYKTT VRSNYYSLYG LYTPSNQNIF TSIIGIYGIS DIINFNQIKN THKAYTSTIQ VMLGSNYKLF STTHIKPMLG LKYSKIREAS YLEDNLSIAS VKERTXEGIL XXKXANSNTL KSVIITPEIY AFAHKILSQK YRXLNXXYRX XXXLXXXXXT XXFXXGXKXX XXXXXXXXXX XXXXXXXXXX XXXXXNXXXX XXXXXXXX
Validated NT Sequence
cnnnnnnnnt tttgtttaac tttaagaagg agnnncatgg ctcaccacca ccaccaccat atgttgaccc agcataattt tgatgcaaat aatagtttaa acgaaaatat agataattta gctaaaaatg aagtagctga taatcataat ctaactgatg aaagcaaaat aaatagtact actgaaaaag actttgaaaa ttctgaaaat aatttagtac attatgctaa caatagctta acatcaacga aagattctac aaccacagaa tttgcaccac gtagagtggt taataatacc gatattttaa tatctaatat tgataataga ttaaattcat taacaagaag ttttgcagca ttagccgcag gagatgaaga acaaatttat ggattttata ttaatccatt tataggcaaa gcaactcaaa aagaaacaaa agaaataagt agatatcata gtacaagtaa aggcacaata atagggcatg atgcacactt aaacgattct ttgcaacttg gagcaaattt tactagagta gaaacaaaat ttaattttcc aagatataaa actacagtta gaagtaatta ctactcattg tatggactat atacaccttc aaaccaaaac atctttacaa gtataattgg aatatatgga attagtgata taattaactt taatcaaata aaaaatactc acaaagccta tactagtact atacaagtaa tgcttggctc taactataag ttatttagta ctacgcatat taagcctatg ttaggactta aatatagcaa aattagagaa gcatcatatt tagaagataa tttatcaata gcaagtgtta aagagcggac gantgaaggt atcttanggg naaaantagc aaattcaaat acactaaaat cagtaattat tactcctgaa atatatgctt ttgcccataa aatattaagt caaaaatacc gagngctcaa caannnttat agannnanan nntanctnnn nnnnncnnnn nnnacnttna nttttantta nggggnaaaa ntnannnnna nncnaanncn nnganannnn aannaatnnn nangnnnnna gnnnnnnnnn nnnncgcnnt ngnnnnnnnt naatnnnnna nnnnnnnann nnnnnncgnn nnnnnnnnnn nnc
Expected Protein Sequence
MAHHHHHHML TQHNFDANNS LNENIDNLAK NEVADNHNLT DESKINSTTE KDFENSENNL VHYANNSLTS TKDSTTTEFA PRRVVNNTDI LISNIDNRLN SLTRSFAALA AGDEEQIYGF YINPFIGKAT QKETKEISRY HSTSKGTIIG HDAHLNDSLQ LGANFTRVET KFNFPRYKTT VRSNYYSLYG LYTPSNQNIF TSIIGIYGIS DIINFNQIKN THKAYTSTIQ VMLGSNYKLF STTHIKPMLG LKYSKIREAS YLEDNLSIAS VKERTIEGIL GVKLANSNTL KSVIITPEIY AFAHKNIKSK IPSAQTNIID NRILPIYIRK LPLSFSYGAK LSLKTKSIEI GINYNGTKAN KFISHLGSIN FKINL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga cccagcataa ttttgatgca aataatagtt taaacgaaaa tatagataat ttagctaaaa atgaagtagc tgataatcat aatctaactg atgaaagcaa aataaatagt actactgaaa aagactttga aaattctgaa aataatttag tacattatgc taacaatagc ttaacatcaa cgaaagattc tacaaccaca gaatttgcac cacgtagagt ggttaataat accgatattt taatatctaa tattgataat agattaaatt cattaacaag aagttttgca gcattagccg caggagatga agaacaaatt tatggatttt atattaatcc atttataggc aaagcaactc aaaaagaaac aaaagaaata agtagatatc atagtacaag taaaggcaca ataatagggc atgatgcaca cttaaacgat tctttgcaac ttggagcaaa ttttactaga gtagaaacaa aatttaattt tccaagatat aaaactacag ttagaagtaa ttactactca ttgtatggac tatatacacc ttcaaaccaa aacatcttta caagtataat tggaatatat ggaattagtg atataattaa ctttaatcaa ataaaaaata ctcacaaagc ctatactagt actatacaag taatgcttgg ctctaactat aagttattta gtactacgca tattaagcct atgttaggac ttaaatatag caaaattaga gaagcatcat atttagaaga taatttatca atagcaagtg ttaaagagcg gacgattgaa ggtatcttag gggtaaaatt agcaaattca aatacactaa aatcagtaat tattactcct gaaatatatg cttttgccca taaaaatatt aagtcaaaaa taccgagtgc tcaaacaaat attatagata atagaatatt acctatatat atcaggaaat tacctttaag ttttagttat ggggcaaaat taagtcttaa aacaaaatca attgagatag gaataaatta taatggaact aaagcaaata aatttatatc gcatttagga agtataaact tcaaaattaa tttatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat