RibeA.01654.a

Cell surface antigen Sca9

CENTER ID: RibeA.01654.a
ORGANISM: Rickettsia bellii RML369-C
ASSOCIATED DISEASE: Typhus
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.01654.a.B2.GE35551 Mature protein( RibeA.01654.a ) 30 354
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|336407.7.peg.168
RefSeq: YP_537316.1
UniProt: Q1RK87

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSQKNNIKD FVKKALVSTS MAATMLTAES AFGTSTLINR NVIGSSADLN SGAGLDAGTP LLPGQMLTVP IQSNINVNTP TVNGTYLSAS GTVMTFTNPN TTIDAIQSSN GNPNALSMVI TPGVHVTLTG VGYPADPDAK DASGNSNPFA GTATNQSDIA SIDFQNSNGI IEMNAKTSGL MDFATGTTPT IIANAADATT IIGAGTIIQV KNQSWTTMGT IDIGQNSQYK YINNLDNNVN VYPTGQTFIF RGPTSVVDFS NESNLSDAFV SDKPIAPITD NSGAITDSYG TAAFHVSTSE TTIAVNIGLS NKQRLNTFIT DGKNITTITG NIFAQNIQIA QNPSGVDPDT TQLI
NT Sequence
ATGTCAAGTC AAAAAAATAA CATAAAGGAT TTCGTAAAAA AGGCACTAGT TAGTACTTCT ATGGCTGCAA CAATGCTTAC AGCAGAAAGT GCTTTCGGTA CATCAACGTT AATAAATCGA AATGTGATAG GAAGTAGTGC AGATTTAAAC AGCGGGGCGG GACTTGATGC AGGAACACCA CTTCTCCCTG GTCAAATGCT TACAGTTCCA ATACAGTCCA ACATCAATGT TAACACGCCC ACGGTTAACG GAACATATCT AAGTGCTTCA GGTACTGTTA TGACCTTTAC AAATCCTAAT ACAACAATTG ATGCTATTCA AAGCTCAAAT GGTAACCCGA ACGCATTAAG TATGGTTATA ACGCCAGGAG TACATGTTAC CCTGACAGGT GTAGGATATC CCGCTGATCC AGATGCAAAA GATGCTTCCG GTAACTCAAA TCCTTTTGCT GGTACTGCAA CTAATCAAAG TGATATCGCA AGTATAGATT TCCAAAACAG TAATGGAATA ATCGAAATGA ATGCCAAAAC TTCTGGCTTA ATGGATTTTG CTACTGGCAC TACACCTACT ATTATTGCTA ATGCTGCGGA TGCTACTACA ATAATCGGGG CTGGCACTAT TATACAGGTA AAAAACCAAT CTTGGACTAC CATGGGAACA ATTGATATTG GTCAAAATTC ACAATATAAA TATATTAACA ACTTAGATAA TAATGTTAAT GTATATCCAA CCGGGCAAAC TTTTATATTT CGAGGTCCAA CTTCTGTAGT TGATTTTAGT AATGAAAGCA ATTTAAGCGA TGCATTTGTA TCCGATAAAC CTATAGCACC TATCACTGAT AACAGTGGAG CAATAACAGA CAGCTATGGG ACAGCAGCCT TTCACGTTTC TACCTCAGAA ACAACTATTG CTGTAAATAT CGGACTTAGC AATAAGCAAA GACTAAATAC TTTTATTACT GATGGTAAGA ATATAACAAC CATTACTGGT AATATTTTTG CTCAAAATAT TCAAATTGCT CAAAACCCTT CAGGGGTAGA TCCCGATACT ACACAGCTTA TT
Details for RibeA.01654.a.B2.GE35551
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 34kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS AFGTSTLINR NVIGSSADLN SGAGLDAGTP LLPGQMLTVP IQSNINVNTP TVNGTYLSAS GTVMTFTNPN TTIDAIQSSN GNPNALSMVI TPGVHVTLTG VGYPADPDAK DASGNSNPFA GTATNQSDIA SIDFQNSNGI IEMNAKTSGL MDFATGTTPT IIANAADATT IIGAGTIIQV KNQSWTTMGT IDIGQNSQYK YINNLDNNVN VYPTGQTFIF RGPTSVVDFS NESNLSDAFV SDKPIAPITD NSGAITDSYG TAAFHVSTSE TTIAVNIGLS NKQRLNTFIT DGKNXTTITG XIFAQNSXCS KXFXGXSRYX XXXXXX
Validated NT Sequence
ttgtnnnnnt taagaaggag atataccatg gctcaccacc accaccacca tatgagtgct ttcggtacat caacgttaat aaatcgaaat gtgataggaa gtagtgcaga tttaaacagc ggggcgggac ttgatgcagg aacaccactt ctccctggtc aaatgcttac agttccaata cagtccaaca tcaatgttaa cacgcccacg gttaacggaa catatctaag tgcttcaggt actgttatga cctttacaaa tcctaataca acaattgatg ctattcaaag ctcaaatggt aacccgaacg cattaagtat ggttataacg ccaggagtac atgttaccct gacaggtgta ggatatcccg ctgatccaga tgcaaaagat gcttccggta actcaaatcc ttttgctggt actgcaacta atcaaagtga tatcgcaagt atagatttcc aaaacagtaa tggaataatc gaaatgaatg ccaaaacttc tggcttaatg gattttgcta ctggcactac acctactatt attgctaatg ctgcggatgc tactacaata atcggggctg gcactattat acaggtaaaa aaccaatctt ggactaccat gggaacaatt gatattggtc aaaattcaca atataaatat attaacaact tagataataa tgttaatgta tatccaaccg ggcaaacttt tatatttcga ggtccaactt ctgtagttga ttttagtaat gaaagcaatt taagcgatgc atttgtatcc gataaaccta tagcacctat cactgataac agtggagcaa taacagacag ctatgggaca gcagcctttc acgtttctac ctcagaaaca actattgctg taaatatcgg acttagcaat aagcaaagac taaatacttt tattactgat ggtaagaata naacaaccat tactggnaan atttttgctc aaaattcaan ttgctcaaaa nccttcnngg gtanatcccg atactannna nnntanttna nnnngtaana nagnnnccgg ctgcnnnnna nnncc
Expected Protein Sequence
MAHHHHHHMS AFGTSTLINR NVIGSSADLN SGAGLDAGTP LLPGQMLTVP IQSNINVNTP TVNGTYLSAS GTVMTFTNPN TTIDAIQSSN GNPNALSMVI TPGVHVTLTG VGYPADPDAK DASGNSNPFA GTATNQSDIA SIDFQNSNGI IEMNAKTSGL MDFATGTTPT IIANAADATT IIGAGTIIQV KNQSWTTMGT IDIGQNSQYK YINNLDNNVN VYPTGQTFIF RGPTSVVDFS NESNLSDAFV SDKPIAPITD NSGAITDSYG TAAFHVSTSE TTIAVNIGLS NKQRLNTFIT DGKNITTITG NIFAQNIQIA QNPSGVDPDT TQLI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtg ctttcggtac atcaacgtta ataaatcgaa atgtgatagg aagtagtgca gatttaaaca gcggggcggg acttgatgca ggaacaccac ttctccctgg tcaaatgctt acagttccaa tacagtccaa catcaatgtt aacacgccca cggttaacgg aacatatcta agtgcttcag gtactgttat gacctttaca aatcctaata caacaattga tgctattcaa agctcaaatg gtaacccgaa cgcattaagt atggttataa cgccaggagt acatgttacc ctgacaggtg taggatatcc cgctgatcca gatgcaaaag atgcttccgg taactcaaat ccttttgctg gtactgcaac taatcaaagt gatatcgcaa gtatagattt ccaaaacagt aatggaataa tcgaaatgaa tgccaaaact tctggcttaa tggattttgc tactggcact acacctacta ttattgctaa tgctgcggat gctactacaa taatcggggc tggcactatt atacaggtaa aaaaccaatc ttggactacc atgggaacaa ttgatattgg tcaaaattca caatataaat atattaacaa cttagataat aatgttaatg tatatccaac cgggcaaact tttatatttc gaggtccaac ttctgtagtt gattttagta atgaaagcaa tttaagcgat gcatttgtat ccgataaacc tatagcacct atcactgata acagtggagc aataacagac agctatggga cagcagcctt tcacgtttct acctcagaaa caactattgc tgtaaatatc ggacttagca ataagcaaag actaaatact tttattactg atggtaagaa tataacaacc attactggta atatttttgc tcaaaatatt caaattgctc aaaacccttc aggggtagat cccgatacta cacagcttat ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat