RibeA.01650.a

Phospholipase D

CENTER ID: RibeA.01650.a
ORGANISM: Rickettsia bellii RML369-C
ASSOCIATED DISEASE: Typhus
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.01650.a.B2.GE35583 Mature protein( RibeA.01650.a ) 25 201
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|336407.7.peg.200
RefSeq: YP_537345.1
UniProt: Q1RK58

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRKNKKFTE IFIAFILGIA IGVLGYSDYG TDLINQFKIS HTPPPKIKHY NISQLSRSKV STCFTPPSGC TKFIADQIDT ARESIYMHAY GMSDSLITAA LIDAQARGVQ VKILLDRSNL KQKFSKLHEL QRAKIEVGID KVPGIAHNKV IIIDKKKVIT GSFNFTAAAD KRNAENVIVI EDAELADSYL QNWLSRKARN G
NT Sequence
GTGAAGAGAA AAAATAAAAA ATTTACGGAA ATATTTATTG CCTTTATTTT AGGCATAGCA ATAGGTGTTT TAGGATATTC AGATTATGGA ACTGATCTAA TAAATCAGTT CAAAATATCG CATACACCGC CTCCAAAAAT AAAGCATTAC AATATTTCGC AGCTTTCTAG AAGTAAAGTT AGTACATGTT TTACTCCCCC ATCAGGTTGC ACTAAATTTA TAGCAGATCA GATTGATACG GCTAGAGAGT CTATTTACAT GCATGCATAT GGTATGAGCG ATTCGTTAAT TACTGCGGCT TTAATTGATG CTCAAGCACG TGGTGTGCAA GTTAAGATCT TACTTGATCG TAGTAATTTA AAGCAAAAAT TTTCTAAATT ACACGAATTA CAACGAGCAA AAATTGAGGT AGGTATAGAT AAAGTTCCGG GTATTGCTCA TAATAAAGTT ATAATTATCG ATAAAAAGAA AGTAATAACC GGTTCATTTA ATTTTACTGC CGCTGCTGAT AAACGTAATG CAGAAAATGT AATCGTTATA GAAGATGCTG AGCTTGCAGA TTCTTATCTA CAAAACTGGC TCAGTAGAAA AGCAAGAAAT GGA
Details for RibeA.01650.a.B2.GE35583
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 20kDa
OBSERVED MW: 20kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG YSDYGTDLIN QFKISHTPPP KIKHYNISQL SRSKVSTCFT PPSGCTKFIA DQIDTARESI YMHAYGMSDS LITAALIDAQ ARGVQVKILL DRSNLKQKFS KLHELQRAKI EVGIDKVPGI AHNKVIIIDK KKVITGSFNF TAAADKRNAE NVIVIEDAEL ADSYLQNWLS RKARNG
Validated NT Sequence
tttgnnnnnn ttaagaagga ganataccat ggctcaccac caccaccacc atatgggata ttcagattat ggaactgatc taataaatca gttcaaaata tcgcatacac cgcctccaaa aataaagcat tacaatattt cgcagctttc tagaagtaaa gttagtacat gttttactcc cccatcaggt tgcactaaat ttatagcaga tcagattgat acggctagag agtctattta catgcatgca tatggtatga gcgattcgtt aattactgcg gctttaattg atgctcaagc acgtggtgtg caagttaaga tcttacttga tcgtagtaat ttaaagcaaa aattttctaa attacacgaa ttacaacgag caaaaattga ggtaggtata gataaagttc cgggtattgc tcataataaa gttataatta tcgataaaaa gaaagtaata accggttcat ttaattttac tgccgctgct gataaacgta atgcagaaaa tgtaatcgtt atagaagatg ctgagcttgc agattcttat ctacaaaact ggctcagtag aaaagcaaga aatggataag tgagtaagat aggatccggc tgctaacaag ccccgaaaga nnnnngcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntnnnnnt
Expected Protein Sequence
MAHHHHHHMG YSDYGTDLIN QFKISHTPPP KIKHYNISQL SRSKVSTCFT PPSGCTKFIA DQIDTARESI YMHAYGMSDS LITAALIDAQ ARGVQVKILL DRSNLKQKFS KLHELQRAKI EVGIDKVPGI AHNKVIIIDK KKVITGSFNF TAAADKRNAE NVIVIEDAEL ADSYLQNWLS RKARNG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggat attcagatta tggaactgat ctaataaatc agttcaaaat atcgcataca ccgcctccaa aaataaagca ttacaatatt tcgcagcttt ctagaagtaa agttagtaca tgttttactc ccccatcagg ttgcactaaa tttatagcag atcagattga tacggctaga gagtctattt acatgcatgc atatggtatg agcgattcgt taattactgc ggctttaatt gatgctcaag cacgtggtgt gcaagttaag atcttacttg atcgtagtaa tttaaagcaa aaattttcta aattacacga attacaacga gcaaaaattg aggtaggtat agataaagtt ccgggtattg ctcataataa agttataatt atcgataaaa agaaagtaat aaccggttca tttaatttta ctgccgctgc tgataaacgt aatgcagaaa atgtaatcgt tatagaagat gctgagcttg cagattctta tctacaaaac tggctcagta gaaaagcaag aaatggatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at