SSGCID: Target Page

RibeA.01649.a

Possible hemolysin C

CENTER ID:	RibeA.01649.a
ORGANISM:	Rickettsia bellii RML369-C
ASSOCIATED DISEASE:	Typhus
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RibeA.01649.a.B1.GE35548	Full length( RibeA.01649.a )		1	301

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|336407.7.peg.1477
RefSeq:	YP_538481.1
UniProt:	Q1RGX2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKSSKKEDD SKKNHDNKSI FHLRKLFSPI KSLFSNKVPD DFFSVIKRLK TNSQKMTLDE RNILANLLKL KGKTIEDIMV PRSDIAAIKL TTNIEELNES IKVKIPHTRT LIYDGTLDNI VGFIHIKDLF KALVTKQNFR LKKLIRKHII AAPSMKLLDL LAKMRREKTH IAIVIDEYGG TDGLVTIEDV MEALVGRIDD EHDQKSEYDN YKVINNSTII SNARVEVEVL EEIIGEKLKD DDDEFDTISG LVLTKMGNVP AVGTKINVSE NIEIEVTDAN PRSLKQVKIT LKNSLKRAKS S

NT Sequence

ATGTTAAAAT CTTCAAAAAA AGAAGATGAC AGTAAAAAGA ATCACGATAA TAAGTCAATT TTTCATTTGC GGAAATTATT CTCACCGATA AAATCTTTAT TTTCAAATAA AGTACCGGAT GATTTTTTTA GCGTTATAAA ACGTCTAAAA ACTAATAGTC AGAAAATGAC TTTAGATGAA CGTAATATTT TAGCTAATTT GCTAAAATTA AAAGGCAAAA CCATTGAAGA CATAATGGTA CCACGCTCTG ATATTGCGGC AATAAAACTT ACTACAAATA TAGAAGAATT AAACGAATCT ATTAAGGTAA AAATTCCGCA TACCAGAACT CTAATATATG ATGGTACTTT GGATAATATA GTTGGTTTTA TTCACATCAA AGATCTATTT AAAGCATTAG TTACGAAACA AAATTTTCGC TTAAAAAAGC TAATACGTAA GCATATAATC GCTGCTCCCT CTATGAAGTT ATTGGATTTG CTTGCAAAAA TGCGTCGTGA GAAAACTCAT ATTGCTATAG TTATTGATGA GTATGGCGGT ACTGATGGAT TAGTTACGAT TGAAGATGTT ATGGAAGCAT TAGTTGGGCG AATCGATGAT GAGCATGATC AGAAATCAGA GTATGATAAC TATAAGGTCA TTAATAACTC AACAATTATT TCAAACGCAC GTGTTGAAGT GGAAGTGCTT GAAGAAATAA TCGGCGAAAA GCTAAAAGAC GATGATGATG AGTTCGATAC TATAAGCGGT TTAGTGCTTA CGAAAATGGG CAATGTTCCG GCGGTGGGTA CTAAAATAAA TGTTTCAGAA AATATTGAAA TTGAAGTAAC GGATGCAAAT CCTCGCTCAT TAAAGCAAGT CAAGATTACG CTTAAAAATA GTTTAAAACG TGCTAAATCA TCT

Details for RibeA.01649.a.B1.GE35548

HARVESTED ON:	6/12/2012
SEQUENCED ON:	6/12/2012
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHML KSSKKEDDSK KNHDNKSIFH LRKLFSPIKS LFSNKVPDDF FSVIKRLKTN SQKMTLDERN ILANLLKLKG KTIEDIMVPR SDIAAIKLTT NIEELNESIK VKIPHTRTLI YDGTLDNIVG FIHIKDLFKA LVTKQNFRLK KLIRKHIIAA PSMKLLDLLA KMRREKTHIA IVIDEYGGTD GLVTIEDVME ALVGRIDDEH DQKSEYDNYK VINNSTIISN ARVEVEVLEE IIGEKLKDDD DEFDTISGLV LTKMGNVPAV GTKINVSXNI EIEVTDANPR SLKQVXITLK IV

Validated NT Sequence

annnttttgt ttaactttaa gaaggagana taccatggct caccaccacc accaccatat gttaaaatct tcaaaaaaag aagatgacag taaaaagaat cacgataata agtcaatttt tcatttgcgg aaattattct caccgataaa atctttattt tcaaataaag taccggatga tttttttagc gttataaaac gtctaaaaac taatagtcag aaaatgactt tagatgaacg taatatttta gctaatttgc taaaattaaa aggcaaaacc attgaagaca taatggtacc acgctctgat attgcggcaa taaaacttac tacaaatata gaagaattaa acgaatctat taaggtaaaa attccgcata ccagaactct aatatatgat ggtactttgg ataatatagt tggttttatt cacatcaaag atctatttaa agcattagtt acgaaacaaa attttcgctt aaaaaagcta atacgtaagc atataatcgc tgctccctct atgaagttat tggatttgct tgcaaaaatg cgtcgtgaga aaactcatat tgctatagtt attgatgagt atggcggtac tgatggatta gttacgattg aagatgttat ggaagcatta gttgggcgaa tcgatgatga gcatgatcag aaatcagagt atgataacta taaggtcatt aataactcaa caattatttc aaacgcacgt gttgaagtgg aagtgcttga agaaataatc ggcgaaaagc taaaagacga tgatgatgag ttcgatacta taagcggttt agtgcttacg aaaatgggca atgttccggc ggtgggtact aaaataaatg tttcnnaaaa tattgaaatt gaagtaacgg atgcaaatcc tcgctcatta aagcaagtcn agattacgct taaaatagtt taaaacgtgc tnaatcatct taagtganna nanagnatcn gnctgnnnnn nnncccnnaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttaa aatcttcaaa aaaagaagat gacagtaaaa agaatcacga taataagtca atttttcatt tgcggaaatt attctcaccg ataaaatctt tattttcaaa taaagtaccg gatgattttt ttagcgttat aaaacgtcta aaaactaata gtcagaaaat gactttagat gaacgtaata ttttagctaa tttgctaaaa ttaaaaggca aaaccattga agacataatg gtaccacgct ctgatattgc ggcaataaaa cttactacaa atatagaaga attaaacgaa tctattaagg taaaaattcc gcataccaga actctaatat atgatggtac tttggataat atagttggtt ttattcacat caaagatcta tttaaagcat tagttacgaa acaaaatttt cgcttaaaaa agctaatacg taagcatata atcgctgctc cctctatgaa gttattggat ttgcttgcaa aaatgcgtcg tgagaaaact catattgcta tagttattga tgagtatggc ggtactgatg gattagttac gattgaagat gttatggaag cattagttgg gcgaatcgat gatgagcatg atcagaaatc agagtatgat aactataagg tcattaataa ctcaacaatt atttcaaacg cacgtgttga agtggaagtg cttgaagaaa taatcggcga aaagctaaaa gacgatgatg atgagttcga tactataagc ggtttagtgc ttacgaaaat gggcaatgtt ccggcggtgg gtactaaaat aaatgtttca gaaaatattg aaattgaagt aacggatgca aatcctcgct cattaaagca agtcaagatt acgcttaaaa atagtttaaa acgtgctaaa tcatcttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t