SSGCID: Target Page

RibeA.00893.a

Thioredoxin reductase

CENTER ID:	RibeA.00893.a
ORGANISM:	Rickettsia bellii RML369-C
ASSOCIATED DISEASE:	Typhus
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RibeA.00893.a.B2.GE35588	Mature protein( RibeA.00893.a )		23	310

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|336407.7.peg.505
RefSeq:	YP_537615.1
UniProt:	Q1RJD8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKITTKVLII GSGPAGLSAA IYAARASLNP ILINGIQPGG QLTITTDVEN YPGFAESVQG PWLMEQMRMQ AENVGTKIVN DYVEKVDLSQ RPFKVSTGSR TEYEAESIII CTGAEARWLG IPTEQEFMGF GVSACATCDG FFFKNQKVVV VGGGNSAVEE ALYLTNHASK VTIVHRRDNF RAEKILQERL FKNPKISVIW DHVVEEIVGN NNPKSVTGVK IQNVHTKETS LVNCSGVFVA IGHKPNTALF AEQVTMDNDN YIITTPGSTK TNIEGVFAAG DVQDKIYRQA ITAAGTGCMA ALEAEKFLNK

NT Sequence

ATGAAAATTA CTACTAAAGT ACTAATAATT GGCTCAGGTC CTGCCGGTTT AAGTGCTGCT ATTTATGCAG CAAGGGCATC TTTAAATCCA ATATTAATTA ATGGTATTCA GCCTGGCGGT CAGCTTACTA TTACCACCGA TGTTGAGAAT TATCCAGGAT TTGCAGAAAG CGTACAAGGT CCTTGGCTAA TGGAACAGAT GCGTATGCAA GCTGAAAATG TCGGCACCAA AATTGTTAAC GATTATGTTG AGAAAGTGGA TTTGTCACAA AGACCTTTTA AAGTGTCTAC AGGATCTAGG ACAGAGTACG AAGCTGAAAG CATAATTATT TGTACTGGTG CGGAAGCAAG ATGGCTCGGC ATCCCTACAG AGCAAGAATT TATGGGATTT GGGGTGTCAG CTTGTGCTAC TTGCGATGGT TTCTTTTTCA AGAATCAGAA AGTAGTGGTA GTTGGTGGCG GTAATAGTGC TGTTGAAGAG GCTTTATATC TGACCAATCA CGCAAGCAAA GTTACTATAG TACATAGAAG AGATAATTTT AGAGCTGAGA AAATATTACA AGAGCGGCTA TTTAAAAATC CTAAAATATC TGTGATTTGG GATCATGTAG TAGAAGAGAT TGTTGGTAAT AATAACCCAA AATCCGTTAC TGGCGTTAAA ATCCAAAATG TTCATACCAA AGAGACGAGC TTAGTAAATT GTAGCGGCGT ATTTGTAGCT ATCGGGCATA AACCAAATAC TGCTTTATTT GCAGAGCAAG TTACAATGGA TAACGATAAT TATATTATTA CTACCCCTGG AAGTACTAAA ACTAATATAG AGGGTGTTTT TGCTGCTGGT GATGTTCAGG ATAAAATCTA TAGACAAGCT ATAACCGCAG CAGGTACTGG CTGTATGGCT GCCCTTGAAG CAGAGAAATT TTTGAATAAG

Details for RibeA.00893.a.B2.GE35588

HARVESTED ON:	6/12/2012
SEQUENCED ON:	6/12/2012
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	95
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMA ARASLNPILI NGIQPGGQLT ITTDVENYPG FAESVQGPWL MEQMRMQAEN VGTKIVNDYV EKVDLSQRPF KVSTGSRTEY EAESIIICTG AEARWLGIPT EQEFMGFGVS ACATCDGFFF KNQKVVVVGG GNSAVEEALY LTNHASKVTI VHRRDNFRAE KILQERLFKN PKISVIWDHV VEEIVGNNNP KSVTGVKIQN VHTKETSLVN CSGVFVAIGH KPNTALFAEQ VTMDNDNYII TTPGSTKTNI EGVFAAGDVX XKIYRQAITX AXLXXWLPLK XXNF

Validated NT Sequence

ttttgtttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatggca gcaagggcat ctttaaatcc aatattaatt aatggtattc agcctggcgg tcagcttact attaccaccg atgttgagaa ttatccagga tttgcagaaa gcgtacaagg tccttggcta atggaacaga tgcgtatgca agctgaaaat gtcggcacca aaattgttaa cgattatgtt gagaaagtgg atttgtcaca aagacctttt aaagtgtcta caggatctag gacagagtac gaagctgaaa gcataattat ttgtactggt gcggaagcaa gatggctcgg catccctaca gagcaagaat ttatgggatt tggggtgtca gcttgtgcta cttgcgatgg tttctttttc aagaatcaga aagtagtggt agttggtggc ggtaatagtg ctgttgaaga ggctttatat ctgaccaatc acgcaagcaa agttactata gtacatagaa gagataattt tagagctgag aaaatattac aagagcggct atttaaaaat cctaaaatat ctgtgatttg ggatcatgta gtagaagaga ttgttggtaa taataaccca aaatccgtta ctggcgttaa aatccaaaat gttcatacca aagagacgag cttagtaaat tgtagcggcg tatttgtagc tatcgggcat aaaccaaata ctgctttatt tgcagagcaa gttacaatgg ataacgataa ttatattatt actacccctg gaagtactaa aactaatata gagggtgttt ttgctgctgg tgatgttcnn nataaaatct atagacaagc tataaccnca gcnnnactgg ntgnatggct gcccttgaag cananaaatt tttgantnan taantgagan nnnnnnnnnn tgnnnnnnnn cccnnaannn nnnnnnnngn nnngnnnnnn nt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag caagggcatc tttaaatcca atattaatta atggtattca gcctggcggt cagcttacta ttaccaccga tgttgagaat tatccaggat ttgcagaaag cgtacaaggt ccttggctaa tggaacagat gcgtatgcaa gctgaaaatg tcggcaccaa aattgttaac gattatgttg agaaagtgga tttgtcacaa agacctttta aagtgtctac aggatctagg acagagtacg aagctgaaag cataattatt tgtactggtg cggaagcaag atggctcggc atccctacag agcaagaatt tatgggattt ggggtgtcag cttgtgctac ttgcgatggt ttctttttca agaatcagaa agtagtggta gttggtggcg gtaatagtgc tgttgaagag gctttatatc tgaccaatca cgcaagcaaa gttactatag tacatagaag agataatttt agagctgaga aaatattaca agagcggcta tttaaaaatc ctaaaatatc tgtgatttgg gatcatgtag tagaagagat tgttggtaat aataacccaa aatccgttac tggcgttaaa atccaaaatg ttcataccaa agagacgagc ttagtaaatt gtagcggcgt atttgtagct atcgggcata aaccaaatac tgctttattt gcagagcaag ttacaatgga taacgataat tatattatta ctacccctgg aagtactaaa actaatatag agggtgtttt tgctgctggt gatgttcagg ataaaatcta tagacaagct ataaccgcag caggtactgg ctgtatggct gcccttgaag cagagaaatt tttgaataag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat