RibeA.00810.a

3-octaprenyl-4-hydroxybenzoate carboxy-lyase

CENTER ID:	RibeA.00810.a
ORGANISM:	Rickettsia bellii RML369-C
ASSOCIATED DISEASE:	Typhus
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RibeA.00810.a.B2.GE35582	Mature protein( RibeA.00810.a )		17	186

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_131043
PATRIC ID:	fig\|336407.7.peg.1053
RefSeq:	YP_538096.1
UniProt:	Q1RI07

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKIIIAISG ASGAIYGIRL LEVLKEQNIE THLVISDGAA LTIKAETEYS IEKVKALANH HYSNKDIGAA IASGSFKTSG MIIAPCSMRT LASVAHAMED NLITRAAGVV LKDKRKLVLM TRETPLHIGH IENMLKVANY GGIIAPPIPA FYSKPKEIDD IVNHSIARVL DLFDIETNLI KRWSGL

NT Sequence

ATGAAAAAAA TTATCATAGC AATTTCTGGA GCTTCAGGGG CAATATATGG GATTCGTTTG CTTGAAGTAT TAAAAGAACA AAATATAGAG ACTCATTTAG TTATTTCAGA TGGAGCCGCT CTTACTATAA AAGCTGAAAC TGAATATTCT ATAGAAAAAG TAAAAGCTCT TGCAAATCAT CACTATAGCA ATAAAGATAT AGGAGCTGCT ATTGCAAGTG GTTCGTTTAA AACTTCTGGC ATGATAATAG CACCATGCAG TATGAGGACT TTAGCTAGCG TTGCTCATGC AATGGAAGAT AATTTAATTA CTAGAGCAGC AGGAGTAGTA TTAAAAGATA AAAGAAAGCT CGTTTTAATG ACTCGCGAAA CACCTCTGCA TATAGGACAT ATTGAAAATA TGCTGAAAGT AGCAAATTAT GGCGGAATTA TAGCACCACC AATTCCGGCT TTTTATAGCA AACCTAAAGA GATAGATGAT ATAGTAAACC ACTCTATCGC TAGAGTTTTA GACCTTTTCG ATATCGAAAC TAATCTGATT AAAAGATGGA GTGGGCTT

Details for RibeA.00810.a.B2.GE35582

HARVESTED ON:	6/12/2012
SEQUENCED ON:	6/12/2012
EXPECTED MW:	19kDa
OBSERVED MW:	19kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG IRLLEVLKEQ NIETHLVISD GAALTIKAET EYSIEKVKAL ANHHYSNKDI GAAIASGSFK TSGMIIAPCS MRTLASVAHA MEDNLITRAA GVVLKDKRKL VLMTRETPLH IGHIENMLKV ANYGGIIAPP IPAFYSKPKE IDDIVNHSIA RVLDLFDIET NLIKRWSGL

Validated NT Sequence

ttgtttactt taagaaggag atataccatg gctcaccacc accaccacca tatggggatt cgtttgcttg aagtattaaa agaacaaaat atagagactc atttagttat ttcagatgga gccgctctta ctataaaagc tgaaactgaa tattctatag aaaaagtaaa agctcttgca aatcatcact atagcaataa agatatagga gctgctattg caagtggttc gtttaaaact tctggcatga taatagcacc atgcagtatg aggactttag ctagcgttgc tcatgcaatg gaagataatt taattactag agcagcagga gtagtattaa aagataaaag aaagctcgtt ttaatgactc gcgaaacacc tctgcatata ggacatattg aaaatatgct gaaagtagca aattatggcg gaattatagc accaccaatt ccggcttttt atagcaaacc taaagagata gatgatatag taaaccactc tatcgctaga gttttagacc ttttcgatat cgaaactaat ctgattaaaa gatggagtgg gctttaagtg agtaagatag gatccggctg ctaacaagcc ccngaangnn nnnngnnnnt nnnnnnnnnn nnnnnnnnnn nnnnngnntn nna

Expected Protein Sequence

MAHHHHHHMG IRLLEVLKEQ NIETHLVISD GAALTIKAET EYSIEKVKAL ANHHYSNKDI GAAIASGSFK TSGMIIAPCS MRTLASVAHA MEDNLITRAA GVVLKDKRKL VLMTRETPLH IGHIENMLKV ANYGGIIAPP IPAFYSKPKE IDDIVNHSIA RVLDLFDIET NLIKRWSGL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggga ttcgtttgct tgaagtatta aaagaacaaa atatagagac tcatttagtt atttcagatg gagccgctct tactataaaa gctgaaactg aatattctat agaaaaagta aaagctcttg caaatcatca ctatagcaat aaagatatag gagctgctat tgcaagtggt tcgtttaaaa cttctggcat gataatagca ccatgcagta tgaggacttt agctagcgtt gctcatgcaa tggaagataa tttaattact agagcagcag gagtagtatt aaaagataaa agaaagctcg ttttaatgac tcgcgaaaca cctctgcata taggacatat tgaaaatatg ctgaaagtag caaattatgg cggaattata gcaccaccaa ttccggcttt ttatagcaaa cctaaagaga tagatgatat agtaaaccac tctatcgcta gagttttaga ccttttcgat atcgaaacta atctgattaa aagatggagt gggctttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t