SSGCID: Target Page

RibeA.00738.a

RNA polymerase sigma factor

CENTER ID:	RibeA.00738.a
ORGANISM:	Rickettsia bellii RML369-C
ASSOCIATED DISEASE:	Typhus
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RibeA.00738.a.B1.GE35550	Full length( RibeA.00738.a )		1	294

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|336407.7.peg.600
RefSeq:	YP_537696.1
UniProt:	Q1RJ57

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSNINALAI SSESGFYSYL QKINKIPSLT QEEEFLLAKS YLEQNDLQAA HKLVTSHLKL VAKIATGYRT YGLPVTELVS EGNIGLMQAV KKFNPELGFR LSTYAMWWIK AAIQEYILKS WSLVKMGTTA AQKKLFFSLS KIKNKITNLY SRAITSDDFV QIADELGVSV SEVSDMNIRL SGSDLSLNNT INNDDASSGE LIELLPETRP SPEAIAISKE DMTHKRALLS SAMKVLNARE LRILTERKLQ DTPKTLDVLS SEYNISKERI RQIENTAFEK IKKFILSSDA IKAN

NT Sequence

ATGACTAGTA ATATTAATGC ATTAGCAATC TCTAGTGAAT CTGGCTTTTA TAGCTACTTA CAGAAAATTA ATAAAATTCC TTCGCTAACG CAAGAAGAAG AATTTTTGCT GGCTAAATCT TATTTAGAGC AGAATGATCT GCAAGCTGCA CATAAGCTAG TAACTAGCCA TCTTAAGTTA GTTGCAAAAA TTGCAACTGG TTATAGAACT TATGGTCTTC CTGTTACCGA ATTAGTATCG GAAGGTAATA TAGGTTTAAT GCAAGCAGTG AAAAAATTCA ATCCTGAGCT TGGTTTTCGT TTATCAACTT ATGCTATGTG GTGGATTAAA GCAGCTATTC AAGAATATAT ATTAAAATCT TGGTCATTAG TAAAGATGGG TACGACTGCT GCACAGAAAA AATTATTTTT TAGCCTCAGT AAAATTAAAA ATAAAATTAC TAATTTATAT TCAAGAGCTA TTACAAGCGA TGATTTTGTC CAAATCGCTG ATGAATTAGG CGTTTCAGTT AGTGAAGTGT CCGATATGAA TATAAGGCTT TCCGGTTCTG ATTTATCGTT AAATAATACT ATAAATAATG ATGATGCAAG TTCAGGCGAG TTGATAGAAT TACTGCCTGA AACACGCCCA AGCCCTGAAG CTATAGCCAT TAGTAAAGAA GATATGACTC ATAAGAGAGC TTTATTATCC AGTGCTATGA AAGTTTTAAA TGCCAGAGAA TTACGTATTT TAACAGAGCG TAAATTGCAA GATACTCCTA AAACTCTTGA TGTTTTAAGT AGTGAATATA ATATTTCTAA AGAACGTATT AGACAAATAG AAAATACTGC TTTTGAGAAG ATAAAGAAAT TTATTTTAAG TAGCGATGCT ATTAAAGCTA AT

Details for RibeA.00738.a.B1.GE35550

HARVESTED ON:	6/12/2012
SEQUENCED ON:	6/12/2012
EXPECTED MW:	33kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT SNINALAISS ESGFYSYLQK INKIPSLTQE EEFLLAKSYL EQNDLQAAHK LVTSHLKLVA KIATGYRTYG LPVTELVSEG NIGLMQAVKK FNPELGFRLS TYAMWWIKAA IQEYILKSWS LVKMGTTAAQ KKLFFSLSKI KNKITNLYSR AITSDDFVQI ADELGVSVSE VSDMNIRLSG SDLSLNNTIN NDDASSGELI ELLPETRPSP EAIAISKEDM THKRALLSSA MKVLNARELR ILTERKLQDT PKTLDVLSSE YNISKERIXQ IXXTAFEKIK KFILSSDAIK AN

Validated NT Sequence

ttgnnnnntt taagaaggag atataccatg gctcaccacc accaccacca tatgactagt aatattaatg cattagcaat ctctagtgaa tctggctttt atagctactt acagaaaatt aataaaattc cttcgctaac gcaagaagaa gaatttttgc tggctaaatc ttatttagag cagaatgatc tgcaagctgc acataagcta gtaactagcc atcttaagtt agttgcaaaa attgcaactg gttatagaac ttatggtctt cctgttaccg aattagtatc ggaaggtaat ataggtttaa tgcaagcagt gaaaaaattc aatcctgagc ttggttttcg tttatcaact tatgctatgt ggtggattaa agcagctatt caagaatata tattaaaatc ttggtcatta gtaaagatgg gtacgactgc tgcacagaaa aaattatttt ttagcctcag taaaattaaa aataaaatta ctaatttata ttcaagagct attacaagcg atgattttgt ccaaatcgct gatgaattag gcgtttcagt tagtgaagtg tccgatatga atataaggct ttccggttct gatttatcgt taaataatac tataaataat gatgatgcaa gttcaggcga gttgatagaa ttactgcctg aaacacgccc aagccctgaa gctatagcca ttagtaaaga agatatgact cataagagag ctttattatc cagtgctatg aaagttttaa atgccagaga attacgtatt ttaacagagc gtaaattgca agatactcct aaaactcttg atgttttaag tagtgaatat aatatttcta aagaacgtat tanacaaata naanatactg cttttgagaa gataaagaaa tttattttaa gtagcgatgc tattaaagct aattaagtga gtaagatagn ancnnntnnn nnnnnnnccn nnnaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacta gtaatattaa tgcattagca atctctagtg aatctggctt ttatagctac ttacagaaaa ttaataaaat tccttcgcta acgcaagaag aagaattttt gctggctaaa tcttatttag agcagaatga tctgcaagct gcacataagc tagtaactag ccatcttaag ttagttgcaa aaattgcaac tggttataga acttatggtc ttcctgttac cgaattagta tcggaaggta atataggttt aatgcaagca gtgaaaaaat tcaatcctga gcttggtttt cgtttatcaa cttatgctat gtggtggatt aaagcagcta ttcaagaata tatattaaaa tcttggtcat tagtaaagat gggtacgact gctgcacaga aaaaattatt ttttagcctc agtaaaatta aaaataaaat tactaattta tattcaagag ctattacaag cgatgatttt gtccaaatcg ctgatgaatt aggcgtttca gttagtgaag tgtccgatat gaatataagg ctttccggtt ctgatttatc gttaaataat actataaata atgatgatgc aagttcaggc gagttgatag aattactgcc tgaaacacgc ccaagccctg aagctatagc cattagtaaa gaagatatga ctcataagag agctttatta tccagtgcta tgaaagtttt aaatgccaga gaattacgta ttttaacaga gcgtaaattg caagatactc ctaaaactct tgatgtttta agtagtgaat ataatatttc taaagaacgt attagacaaa tagaaaatac tgcttttgag aagataaaga aatttatttt aagtagcgat gctattaaag ctaattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat