RibeA.00369.a

Penicillin-binding protein dacF

CENTER ID: RibeA.00369.a
ORGANISM: Rickettsia bellii RML369-C
ASSOCIATED DISEASE: Typhus
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.00369.a.B2.GE35592 Mature protein( RibeA.00369.a ) 23 307
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.00369.a.B2.PS01607 Mature protein( RibeA.00369.a ) 23 307
RibeA.00369.a.B2.PS01608 Mature protein( RibeA.00369.a ) 23 307

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|336407.7.peg.1059
RefSeq: YP_538101.1
UniProt: Q1RI02

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFRKILLPYI FVIISLLTQV SEAKKAVKNS SKVPIPVQTS LVIDAKTGKV LHAHNSQIRI YPASLTKLMT LYVMFELIEL GKLSLDKKLL VSAKAAKMPP CKIGLRAGDT ITTREAINML IVKSANDAAV TVAENIRLSE KDFAHLMNIK AKQLGMKNTY FKNASGWHDP LQQTTARDMA KLTLAIKQKF PKYYPLFSKT SFVFRGNVIN GHNKITKNYA GAEGMKTGFH TPAGYNLVTT ASRNGKSLIA VVTGGRSAGS RDQKMIGLLD QHFNVASAKP KVTLASNNKV KAKPKPKIVI KKGVMRS
NT Sequence
ATGTTTAGAA AAATTTTATT GCCTTACATT TTTGTTATCA TTTCTTTACT TACCCAAGTA AGTGAAGCAA AAAAGGCTGT AAAAAATTCT TCTAAAGTTC CTATTCCCGT TCAAACCAGC TTAGTAATTG ATGCAAAAAC CGGTAAAGTT TTGCACGCTC ATAATTCTCA AATCCGAATT TATCCCGCCT CTTTAACTAA GTTAATGACT TTATATGTGA TGTTTGAGTT AATTGAATTA GGTAAATTAT CTTTAGATAA AAAGCTTCTT GTATCGGCAA AAGCAGCAAA AATGCCTCCC TGTAAAATTG GTTTAAGGGC TGGGGATACT ATAACAACTA GAGAAGCAAT CAATATGCTA ATTGTTAAAT CAGCAAATGA TGCTGCTGTT ACGGTTGCTG AAAATATTAG ACTTTCTGAA AAAGACTTTG CTCACTTAAT GAATATTAAA GCCAAGCAGC TTGGAATGAA AAATACTTAT TTTAAAAATG CTTCAGGTTG GCATGACCCA TTACAGCAAA CTACAGCACG AGATATGGCT AAGCTTACTT TAGCGATTAA ACAAAAATTC CCTAAATATT ATCCGTTATT TTCTAAAACT AGTTTTGTGT TTCGTGGTAA TGTAATAAAT GGTCATAATA AGATTACTAA AAATTATGCA GGAGCTGAAG GTATGAAAAC AGGTTTTCAT ACTCCAGCAG GATATAATTT AGTTACTACT GCCTCAAGAA ACGGTAAATC ATTAATCGCA GTTGTTACAG GTGGTAGAAG TGCTGGATCT CGTGATCAGA AAATGATCGG GTTATTAGAT CAGCACTTTA ACGTAGCATC TGCCAAACCT AAAGTCACAC TTGCTTCTAA CAATAAAGTA AAAGCAAAAC CTAAGCCAAA AATAGTGATA AAAAAAGGAG TCATGCGTAG T
Details for RibeA.00369.a.B2.GE35592
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAKKAVKNSS KVPIPVQTSL VIDAKTGKVL HAHNSQIRIY PASLTKLMTL YVMFELIELG KLSLDKKLLV SAKAAKMPPC KIGLRAGDTI TTREAINMLI VKSANDAAVT VAENIRLSEK DFAHLMNIKA KQLGMKNTYF KNASGWHDPL QQTTARDMAK LTLAIKQKFP KYYPLFSKTS FVFRGNVING HNKITKNYAG AEGMKTGFHT PAGYNLVTTA SRNGKSLIAV VTGGRSAGSR DQKMIGLLDQ HFNVASAKPK VTLASNNKVK AKPKPKIVIK KGVMRS
Validated NT Sequence
tcttactcac ttaactacgc atgactcctt tttttatcac tatttttggc ttaggttttg cttttacttt attgttagaa gcaagtgtga ctttaggttt ggcagatgct acgttaaagt gctgatctaa taacccgatc attttctgat cacgagatcc agcacttcta ccacctgtaa caactgcgat taatgattta ccgtttcttg aggcagtagt aactaaatta tatcctgctg gagtatgaaa acctgttttc ataccttcag ctcctgcata atttttagta atcttattat gaccatttat tacattacca cgaaacacaa aactagtttt agaaaataac ggataatatt tagggaattt ttgtttaatc gctaaagtaa gcttagccat atctcgtgct gtagtttgct gtaatgggtc atgccaacct gaagcatttt taaaataagt atttttcatt ccaagctgct tggctttaat attcattaag tgagcaaagt ctttttcaga aagtctaata ttttcagcaa ccgtaacagc agcatcattt gctgatttaa caattagcat attgattgct tctctagttg ttatagtatc cccagccctt aaaccaattt tacagggagg catttttgct gcttttgccg atacaagaag ctttttatct aaagataatt tacctaattc aattaactca aacatcacat ataaagtcat taacttagtt aaagaggcgg gataaattcg gatttgagaa ttatgagcgt gcaaaacttt accggttttt gcatcaatta ctaagctggt ttgaacggga ataggaactt tagaagaatt ttttacagcc ttttttgcca tatggtggtg gtggtggtga gcca
Expected Protein Sequence
MAHHHHHHMA KKAVKNSSKV PIPVQTSLVI DAKTGKVLHA HNSQIRIYPA SLTKLMTLYV MFELIELGKL SLDKKLLVSA KAAKMPPCKI GLRAGDTITT REAINMLIVK SANDAAVTVA ENIRLSEKDF AHLMNIKAKQ LGMKNTYFKN ASGWHDPLQQ TTARDMAKLT LAIKQKFPKY YPLFSKTSFV FRGNVINGHN KITKNYAGAE GMKTGFHTPA GYNLVTTASR NGKSLIAVVT GGRSAGSRDQ KMIGLLDQHF NVASAKPKVT LASNNKVKAK PKPKIVIKKG VMRS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa aaaaggctgt aaaaaattct tctaaagttc ctattcccgt tcaaaccagc ttagtaattg atgcaaaaac cggtaaagtt ttgcacgctc ataattctca aatccgaatt tatcccgcct ctttaactaa gttaatgact ttatatgtga tgtttgagtt aattgaatta ggtaaattat ctttagataa aaagcttctt gtatcggcaa aagcagcaaa aatgcctccc tgtaaaattg gtttaagggc tggggatact ataacaacta gagaagcaat caatatgcta attgttaaat cagcaaatga tgctgctgtt acggttgctg aaaatattag actttctgaa aaagactttg ctcacttaat gaatattaaa gccaagcagc ttggaatgaa aaatacttat tttaaaaatg cttcaggttg gcatgaccca ttacagcaaa ctacagcacg agatatggct aagcttactt tagcgattaa acaaaaattc cctaaatatt atccgttatt ttctaaaact agttttgtgt ttcgtggtaa tgtaataaat ggtcataata agattactaa aaattatgca ggagctgaag gtatgaaaac aggttttcat actccagcag gatataattt agttactact gcctcaagaa acggtaaatc attaatcgca gttgttacag gtggtagaag tgctggatct cgtgatcaga aaatgatcgg gttattagat cagcacttta acgtagcatc tgccaaacct aaagtcacac ttgcttctaa caataaagta aaagcaaaac ctaagccaaa aatagtgata aaaaaaggag tcatgcgtag ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for RibeA.00369.a.B2.PS01607
PURIFICATION DATe: 8/22/2012
CONCENTRATION: 20.4mg/ml
OBSERVED MW: 31kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAKKAVKNSS KVPIPVQTSL VIDAKTGKVL HAHNSQIRIY PASLTKLMTL YVMFELIELG KLSLDKKLLV SAKAAKMPPC KIGLRAGDTI TTREAINMLI VKSANDAAVT VAENIRLSEK DFAHLMNIKA KQLGMKNTYF KNASGWHDPL QQTTARDMAK LTLAIKQKFP KYYPLFSKTS FVFRGNVING HNKITKNYAG AEGMKTGFHT PAGYNLVTTA SRNGKSLIAV VTGGRSAGSR DQKMIGLLDQ HFNVASAKPK VTLASNNKVK AKPKPKIVIK KGVMRS
Validated NT Sequence
tcttactcac ttaactacgc atgactcctt tttttatcac tatttttggc ttaggttttg cttttacttt attgttagaa gcaagtgtga ctttaggttt ggcagatgct acgttaaagt gctgatctaa taacccgatc attttctgat cacgagatcc agcacttcta ccacctgtaa caactgcgat taatgattta ccgtttcttg aggcagtagt aactaaatta tatcctgctg gagtatgaaa acctgttttc ataccttcag ctcctgcata atttttagta atcttattat gaccatttat tacattacca cgaaacacaa aactagtttt agaaaataac ggataatatt tagggaattt ttgtttaatc gctaaagtaa gcttagccat atctcgtgct gtagtttgct gtaatgggtc atgccaacct gaagcatttt taaaataagt atttttcatt ccaagctgct tggctttaat attcattaag tgagcaaagt ctttttcaga aagtctaata ttttcagcaa ccgtaacagc agcatcattt gctgatttaa caattagcat attgattgct tctctagttg ttatagtatc cccagccctt aaaccaattt tacagggagg catttttgct gcttttgccg atacaagaag ctttttatct aaagataatt tacctaattc aattaactca aacatcacat ataaagtcat taacttagtt aaagaggcgg gataaattcg gatttgagaa ttatgagcgt gcaaaacttt accggttttt gcatcaatta ctaagctggt ttgaacggga ataggaactt tagaagaatt ttttacagcc ttttttgcca tatggtggtg gtggtggtga gcca
Expressed Protein Sequence
MAHHHHHHMA KKAVKNSSKV PIPVQTSLVI DAKTGKVLHA HNSQIRIYPA SLTKLMTLYV MFELIELGKL SLDKKLLVSA KAAKMPPCKI GLRAGDTITT REAINMLIVK SANDAAVTVA ENIRLSEKDF AHLMNIKAKQ LGMKNTYFKN ASGWHDPLQQ TTARDMAKLT LAIKQKFPKY YPLFSKTSFV FRGNVINGHN KITKNYAGAE GMKTGFHTPA GYNLVTTASR NGKSLIAVVT GGRSAGSRDQ KMIGLLDQHF NVASAKPKVT LASNNKVKAK PKPKIVIKKG VMRS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAA AAAAGGCTGT AAAAAATTCT TCTAAAGTTC CTATTCCCGT TCAAACCAGC TTAGTAATTG ATGCAAAAAC CGGTAAAGTT TTGCACGCTC ATAATTCTCA AATCCGAATT TATCCCGCCT CTTTAACTAA GTTAATGACT TTATATGTGA TGTTTGAGTT AATTGAATTA GGTAAATTAT CTTTAGATAA AAAGCTTCTT GTATCGGCAA AAGCAGCAAA AATGCCTCCC TGTAAAATTG GTTTAAGGGC TGGGGATACT ATAACAACTA GAGAAGCAAT CAATATGCTA ATTGTTAAAT CAGCAAATGA TGCTGCTGTT ACGGTTGCTG AAAATATTAG ACTTTCTGAA AAAGACTTTG CTCACTTAAT GAATATTAAA GCCAAGCAGC TTGGAATGAA AAATACTTAT TTTAAAAATG CTTCAGGTTG GCATGACCCA TTACAGCAAA CTACAGCACG AGATATGGCT AAGCTTACTT TAGCGATTAA ACAAAAATTC CCTAAATATT ATCCGTTATT TTCTAAAACT AGTTTTGTGT TTCGTGGTAA TGTAATAAAT GGTCATAATA AGATTACTAA AAATTATGCA GGAGCTGAAG GTATGAAAAC AGGTTTTCAT ACTCCAGCAG GATATAATTT AGTTACTACT GCCTCAAGAA ACGGTAAATC ATTAATCGCA GTTGTTACAG GTGGTAGAAG TGCTGGATCT CGTGATCAGA AAATGATCGG GTTATTAGAT CAGCACTTTA ACGTAGCATC TGCCAAACCT AAAGTCACAC TTGCTTCTAA CAATAAAGTA AAAGCAAAAC CTAAGCCAAA AATAGTGATA AAAAAAGGAG TCATGCGTAG TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT
Details for RibeA.00369.a.B2.PS01608
PURIFICATION DATe: 8/22/2012
CONCENTRATION: 27.1mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAKKAVKNSS KVPIPVQTSL VIDAKTGKVL HAHNSQIRIY PASLTKLMTL YVMFELIELG KLSLDKKLLV SAKAAKMPPC KIGLRAGDTI TTREAINMLI VKSANDAAVT VAENIRLSEK DFAHLMNIKA KQLGMKNTYF KNASGWHDPL QQTTARDMAK LTLAIKQKFP KYYPLFSKTS FVFRGNVING HNKITKNYAG AEGMKTGFHT PAGYNLVTTA SRNGKSLIAV VTGGRSAGSR DQKMIGLLDQ HFNVASAKPK VTLASNNKVK AKPKPKIVIK KGVMRS
Validated NT Sequence
tcttactcac ttaactacgc atgactcctt tttttatcac tatttttggc ttaggttttg cttttacttt attgttagaa gcaagtgtga ctttaggttt ggcagatgct acgttaaagt gctgatctaa taacccgatc attttctgat cacgagatcc agcacttcta ccacctgtaa caactgcgat taatgattta ccgtttcttg aggcagtagt aactaaatta tatcctgctg gagtatgaaa acctgttttc ataccttcag ctcctgcata atttttagta atcttattat gaccatttat tacattacca cgaaacacaa aactagtttt agaaaataac ggataatatt tagggaattt ttgtttaatc gctaaagtaa gcttagccat atctcgtgct gtagtttgct gtaatgggtc atgccaacct gaagcatttt taaaataagt atttttcatt ccaagctgct tggctttaat attcattaag tgagcaaagt ctttttcaga aagtctaata ttttcagcaa ccgtaacagc agcatcattt gctgatttaa caattagcat attgattgct tctctagttg ttatagtatc cccagccctt aaaccaattt tacagggagg catttttgct gcttttgccg atacaagaag ctttttatct aaagataatt tacctaattc aattaactca aacatcacat ataaagtcat taacttagtt aaagaggcgg gataaattcg gatttgagaa ttatgagcgt gcaaaacttt accggttttt gcatcaatta ctaagctggt ttgaacggga ataggaactt tagaagaatt ttttacagcc ttttttgcca tatggtggtg gtggtggtga gcca
Expressed Protein Sequence
MAHHHHHHMA KKAVKNSSKV PIPVQTSLVI DAKTGKVLHA HNSQIRIYPA SLTKLMTLYV MFELIELGKL SLDKKLLVSA KAAKMPPCKI GLRAGDTITT REAINMLIVK SANDAAVTVA ENIRLSEKDF AHLMNIKAKQ LGMKNTYFKN ASGWHDPLQQ TTARDMAKLT LAIKQKFPKY YPLFSKTSFV FRGNVINGHN KITKNYAGAE GMKTGFHTPA GYNLVTTASR NGKSLIAVVT GGRSAGSRDQ KMIGLLDQHF NVASAKPKVT LASNNKVKAK PKPKIVIKKG VMRS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAA AAAAGGCTGT AAAAAATTCT TCTAAAGTTC CTATTCCCGT TCAAACCAGC TTAGTAATTG ATGCAAAAAC CGGTAAAGTT TTGCACGCTC ATAATTCTCA AATCCGAATT TATCCCGCCT CTTTAACTAA GTTAATGACT TTATATGTGA TGTTTGAGTT AATTGAATTA GGTAAATTAT CTTTAGATAA AAAGCTTCTT GTATCGGCAA AAGCAGCAAA AATGCCTCCC TGTAAAATTG GTTTAAGGGC TGGGGATACT ATAACAACTA GAGAAGCAAT CAATATGCTA ATTGTTAAAT CAGCAAATGA TGCTGCTGTT ACGGTTGCTG AAAATATTAG ACTTTCTGAA AAAGACTTTG CTCACTTAAT GAATATTAAA GCCAAGCAGC TTGGAATGAA AAATACTTAT TTTAAAAATG CTTCAGGTTG GCATGACCCA TTACAGCAAA CTACAGCACG AGATATGGCT AAGCTTACTT TAGCGATTAA ACAAAAATTC CCTAAATATT ATCCGTTATT TTCTAAAACT AGTTTTGTGT TTCGTGGTAA TGTAATAAAT GGTCATAATA AGATTACTAA AAATTATGCA GGAGCTGAAG GTATGAAAAC AGGTTTTCAT ACTCCAGCAG GATATAATTT AGTTACTACT GCCTCAAGAA ACGGTAAATC ATTAATCGCA GTTGTTACAG GTGGTAGAAG TGCTGGATCT CGTGATCAGA AAATGATCGG GTTATTAGAT CAGCACTTTA ACGTAGCATC TGCCAAACCT AAAGTCACAC TTGCTTCTAA CAATAAAGTA AAAGCAAAAC CTAAGCCAAA AATAGTGATA AAAAAAGGAG TCATGCGTAG TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT