RibeA.00169.a

Replicative DNA helicase

CENTER ID: RibeA.00169.a
ORGANISM: Rickettsia bellii RML369-C
ASSOCIATED DISEASE: Typhus
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.00169.a.B1.GE35589 Full length( RibeA.00169.a ) 1 498
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.00169.a.B1.PW36223 Full length( RibeA.00169.a ) 1 498
RibeA.00169.a.B1.PW36545 Full length( RibeA.00169.a ) 1 498

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|336407.7.peg.1056
RefSeq: YP_538099.1
UniProt: Q1RI04

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARNKTNNDK NIANDEASTV LARVLPSNVQ AEQMLLGAIL TNNELLNYVS EFLRSEHFFE PIHQKIYNGI EVITEKGLIA TPITLRSMLA NDELFHEMEG AEYLAKLVTM SMMVINPIDY GKIIYDLAIK RYLINIGEEV VNDAYNSSLE FEAKEQIERA ESKLYDLASE GINEKSFTKI AIPISESIAS INRAMKNNDH VIGISTGLID LDSKLFGFHN SDLVILAGRP AMGKTAFALN LALNACNNML IKNKRDNEEV QSVGFFSLEM SSEQLTTRLL SMSAGIDSTT LRTGMINEAN YNRLRKEAIA LSELSFFIDD TPALTISAIR TRARRMKRKH NLGILFVDYL QLIRGVSKSD NRVNEVSEIT QGLKAIAKEL NIPVIALSQL SRAVELREDK RPMLSDLRES GSIEQDADIV MFIYREEYYL SRKEPAASDP KHAEKYAEWQ DNMSKAHNIT DIIIAKHRSG PVGNVQLFYD SQYSKFVNLE TKYPVHPR
NT Sequence
ATGGCACGTA ATAAAACAAA CAACGATAAA AATATAGCTA ACGATGAAGC AAGTACTGTA TTAGCAAGAG TTTTACCTTC AAATGTTCAA GCAGAACAAA TGTTGCTTGG AGCAATTTTA ACTAATAATG AATTGCTAAA TTATGTATCA GAATTTTTAC GCAGTGAGCA TTTTTTTGAA CCTATTCACC AAAAAATCTA TAATGGAATT GAAGTAATTA CCGAGAAAGG TTTAATAGCT ACCCCTATAA CATTACGTAG TATGCTAGCA AATGATGAGC TTTTTCATGA AATGGAGGGA GCTGAATATT TAGCAAAATT AGTCACTATG TCAATGATGG TAATTAATCC TATTGACTAT GGTAAAATAA TATATGATTT AGCAATAAAA CGTTATTTAA TAAATATCGG TGAAGAAGTA GTAAACGACG CATATAATTC TTCTTTAGAG TTTGAAGCAA AAGAACAAAT TGAACGAGCC GAATCTAAGC TTTATGATTT AGCTAGTGAA GGGATAAATG AAAAAAGCTT TACCAAAATT GCTATACCCA TTTCAGAGTC GATTGCTAGT ATAAACAGAG CAATGAAAAA TAACGATCAT GTAATAGGTA TATCTACTGG CTTAATTGAT TTAGATAGTA AATTATTTGG TTTTCATAAT TCTGATCTTG TAATTCTTGC AGGACGTCCT GCAATGGGTA AAACAGCCTT TGCTTTAAAT CTAGCCTTAA ATGCTTGCAA TAATATGCTT ATTAAAAATA AGCGTGATAA TGAAGAGGTG CAATCTGTGG GATTTTTCTC TCTAGAAATG TCCTCAGAGC AGCTTACAAC ACGTTTGCTT TCAATGAGTG CAGGAATTGA CTCTACTACT CTTCGTACGG GAATGATTAA TGAAGCAAAT TATAATCGTC TTCGCAAAGA AGCCATAGCT TTATCAGAAT TATCATTTTT TATTGATGAT ACTCCTGCCT TAACTATTTC AGCTATAAGA ACACGTGCTA GAAGAATGAA ACGCAAGCAT AATTTAGGTA TATTATTTGT CGATTATTTA CAATTAATTA GAGGTGTAAG TAAATCCGAT AATAGGGTTA ATGAAGTTTC AGAAATCACC CAAGGTTTAA AAGCTATAGC AAAAGAATTA AATATTCCTG TCATTGCATT ATCTCAGCTA TCAAGAGCCG TAGAGCTTCG TGAGGATAAA AGACCTATGC TATCCGATCT TAGAGAATCA GGAAGTATTG AACAAGATGC CGATATAGTT ATGTTTATTT ACCGAGAAGA ATATTATCTA AGCAGAAAAG AGCCAGCAGC AAGCGATCCT AAACATGCCG AAAAATATGC CGAATGGCAA GATAATATGA GTAAAGCACA TAATATTACC GATATTATAA TTGCAAAACA CCGTAGTGGT CCTGTTGGTA ATGTTCAACT TTTCTATGAC AGTCAATATT CTAAATTTGT TAATCTAGAA ACAAAATATC CAGTGCATCC GAGA
Details for RibeA.00169.a.B1.GE35589
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 57kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 57
Validated AA Sequence
MAHHHHHHMA RNKTNNDKNI ANDEASTVLA RVLPSNVQAE QMLLGAILTN NELLNYVSEF LRSEHFFEPI HQKIYNGIEV ITEKGLIATP ITLRSMLAND ELFHEMEGAE YLAKLVTMSM MVINPIDYGK IIYDLAIKRY LINIGEEVVN DAYNSSLEFE AKEQIERAES KLYDLASEGI NEKSFTKIAI PISESIASIN RAMKNNDHVI XISTGLIDLD SKLFGFHNSD LVILAGRPAM GKTAFALNLA LNACNNMLIK NKRDNEXVQS XGFFXXEMXS XQLTXVXXX
Validated NT Sequence
tttgtnnnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatggcacg taataaaaca aacaacgata aaaatatagc taacgatgaa gcaagtactg tattagcaag agttttacct tcaaatgttc aagcagaaca aatgttgctt ggagcaattt taactaataa tgaattgcta aattatgtat cagaattttt acgcagtgag catttttttg aacctattca ccaaaaaatc tataatggaa ttgaagtaat taccgagaaa ggtttaatag ctacccctat aacattacgt agtatgctag caaatgatga gctttttcat gaaatggagg gagctgaata tttagcaaaa ttagtcacta tgtcaatgat ggtaattaat cctattgact atggtaaaat aatatatgat ttagcaataa aacgttattt aataaatatc ggtgaagaag tagtaaacga cgcatataat tcttctttag agtttgaagc aaaagaacaa attgaacgag ccgaatctaa gctttatgat ttagctagtg aagggataaa tgaaaaaagc tttaccaaaa ttgctatacc catttcagag tcgattgcta gtataaacag agcaatgaaa aataacgatc atgtaatang tatatctact ggcttaattg atttagatag taaattattt ggntttcata attctgatct tgtaattctt gcaggacgtc ctgcaatggg taaaacagcc tttgctttaa atctagcctt aaatgcttgc aataatatgc ttattaaaaa taagcgtgat aatgaagang tgcaatctgn gggatttttc nctcnngaaa tgncctcana gcagcttaca nncgttngcn ttcnatgann gnngnaattg acnnnactac tnntcgtacg ggnatgnnnn annnncaant nnancgtcnt cnnaanaanc cntncttnnn nnnannnnnc nttttttann gatnnnnnnn ncnnnnncnn antnnngcnn nnanaanncn nnnnnnaann aannnnnnnn gcnnantnnn nnnnnnnncn nnnnntnnnn nnnngnnnnn nannnccnnn nnnnnnnnnn nnnnnannnn nnntnnnnnn nnnnnnnnnn aannnnnng
Expected Protein Sequence
MAHHHHHHMA RNKTNNDKNI ANDEASTVLA RVLPSNVQAE QMLLGAILTN NELLNYVSEF LRSEHFFEPI HQKIYNGIEV ITEKGLIATP ITLRSMLAND ELFHEMEGAE YLAKLVTMSM MVINPIDYGK IIYDLAIKRY LINIGEEVVN DAYNSSLEFE AKEQIERAES KLYDLASEGI NEKSFTKIAI PISESIASIN RAMKNNDHVI GISTGLIDLD SKLFGFHNSD LVILAGRPAM GKTAFALNLA LNACNNMLIK NKRDNEEVQS VGFFSLEMSS EQLTTRLLSM SAGIDSTTLR TGMINEANYN RLRKEAIALS ELSFFIDDTP ALTISAIRTR ARRMKRKHNL GILFVDYLQL IRGVSKSDNR VNEVSEITQG LKAIAKELNI PVIALSQLSR AVELREDKRP MLSDLRESGS IEQDADIVMF IYREEYYLSR KEPAASDPKH AEKYAEWQDN MSKAHNITDI IIAKHRSGPV GNVQLFYDSQ YSKFVNLETK YPVHPR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcac gtaataaaac aaacaacgat aaaaatatag ctaacgatga agcaagtact gtattagcaa gagttttacc ttcaaatgtt caagcagaac aaatgttgct tggagcaatt ttaactaata atgaattgct aaattatgta tcagaatttt tacgcagtga gcattttttt gaacctattc accaaaaaat ctataatgga attgaagtaa ttaccgagaa aggtttaata gctaccccta taacattacg tagtatgcta gcaaatgatg agctttttca tgaaatggag ggagctgaat atttagcaaa attagtcact atgtcaatga tggtaattaa tcctattgac tatggtaaaa taatatatga tttagcaata aaacgttatt taataaatat cggtgaagaa gtagtaaacg acgcatataa ttcttcttta gagtttgaag caaaagaaca aattgaacga gccgaatcta agctttatga tttagctagt gaagggataa atgaaaaaag ctttaccaaa attgctatac ccatttcaga gtcgattgct agtataaaca gagcaatgaa aaataacgat catgtaatag gtatatctac tggcttaatt gatttagata gtaaattatt tggttttcat aattctgatc ttgtaattct tgcaggacgt cctgcaatgg gtaaaacagc ctttgcttta aatctagcct taaatgcttg caataatatg cttattaaaa ataagcgtga taatgaagag gtgcaatctg tgggattttt ctctctagaa atgtcctcag agcagcttac aacacgtttg ctttcaatga gtgcaggaat tgactctact actcttcgta cgggaatgat taatgaagca aattataatc gtcttcgcaa agaagccata gctttatcag aattatcatt ttttattgat gatactcctg ccttaactat ttcagctata agaacacgtg ctagaagaat gaaacgcaag cataatttag gtatattatt tgtcgattat ttacaattaa ttagaggtgt aagtaaatcc gataataggg ttaatgaagt ttcagaaatc acccaaggtt taaaagctat agcaaaagaa ttaaatattc ctgtcattgc attatctcag ctatcaagag ccgtagagct tcgtgaggat aaaagaccta tgctatccga tcttagagaa tcaggaagta ttgaacaaga tgccgatata gttatgttta tttaccgaga agaatattat ctaagcagaa aagagccagc agcaagcgat cctaaacatg ccgaaaaata tgccgaatgg caagataata tgagtaaagc acataatatt accgatatta taattgcaaa acaccgtagt ggtcctgttg gtaatgttca acttttctat gacagtcaat attctaaatt tgttaatcta gaaacaaaat atccagtgca tccgagatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for RibeA.00169.a.B1.PW36223
PURIFICATION DATe: 7/30/2012
CONCENTRATION: 2.56mg/ml
OBSERVED MW: 56kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 22
VIAL VOLUME: 200µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 57
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA RNKTNNDKNI ANDEASTVLA RVLPSNVQAE QMLLGAILTN NELLNYVSEF LRSEHFFEPI HQKIYNGIEV ITEKGLIATP ITLRSMLAND ELFHEMEGAE YLAKLVTMSM MVINPIDYGK IIYDLAIKRY LINIGEEVVN DAYNSSLEFE AKEQIERAES KLYDLASEGI NEKSFTKIAI PISESIASIN RAMKNNDHVI XISTGLIDLD SKLFGFHNSD LVILAGRPAM GKTAFALNLA LNACNNMLIK NKRDNEXVQS XGFFXXEMXS XQLTXVXXX
Validated NT Sequence
tttgtnnnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatggcacg taataaaaca aacaacgata aaaatatagc taacgatgaa gcaagtactg tattagcaag agttttacct tcaaatgttc aagcagaaca aatgttgctt ggagcaattt taactaataa tgaattgcta aattatgtat cagaattttt acgcagtgag catttttttg aacctattca ccaaaaaatc tataatggaa ttgaagtaat taccgagaaa ggtttaatag ctacccctat aacattacgt agtatgctag caaatgatga gctttttcat gaaatggagg gagctgaata tttagcaaaa ttagtcacta tgtcaatgat ggtaattaat cctattgact atggtaaaat aatatatgat ttagcaataa aacgttattt aataaatatc ggtgaagaag tagtaaacga cgcatataat tcttctttag agtttgaagc aaaagaacaa attgaacgag ccgaatctaa gctttatgat ttagctagtg aagggataaa tgaaaaaagc tttaccaaaa ttgctatacc catttcagag tcgattgcta gtataaacag agcaatgaaa aataacgatc atgtaatang tatatctact ggcttaattg atttagatag taaattattt ggntttcata attctgatct tgtaattctt gcaggacgtc ctgcaatggg taaaacagcc tttgctttaa atctagcctt aaatgcttgc aataatatgc ttattaaaaa taagcgtgat aatgaagang tgcaatctgn gggatttttc nctcnngaaa tgncctcana gcagcttaca nncgttngcn ttcnatgann gnngnaattg acnnnactac tnntcgtacg ggnatgnnnn annnncaant nnancgtcnt cnnaanaanc cntncttnnn nnnannnnnc nttttttann gatnnnnnnn ncnnnnncnn antnnngcnn nnanaanncn nnnnnnaann aannnnnnnn gcnnantnnn nnnnnnnncn nnnnntnnnn nnnngnnnnn nannnccnnn nnnnnnnnnn nnnnnannnn nnntnnnnnn nnnnnnnnnn aannnnnng
Expressed Protein Sequence
MAHHHHHHMA RNKTNNDKNI ANDEASTVLA RVLPSNVQAE QMLLGAILTN NELLNYVSEF LRSEHFFEPI HQKIYNGIEV ITEKGLIATP ITLRSMLAND ELFHEMEGAE YLAKLVTMSM MVINPIDYGK IIYDLAIKRY LINIGEEVVN DAYNSSLEFE AKEQIERAES KLYDLASEGI NEKSFTKIAI PISESIASIN RAMKNNDHVI GISTGLIDLD SKLFGFHNSD LVILAGRPAM GKTAFALNLA LNACNNMLIK NKRDNEEVQS VGFFSLEMSS EQLTTRLLSM SAGIDSTTLR TGMINEANYN RLRKEAIALS ELSFFIDDTP ALTISAIRTR ARRMKRKHNL GILFVDYLQL IRGVSKSDNR VNEVSEITQG LKAIAKELNI PVIALSQLSR AVELREDKRP MLSDLRESGS IEQDADIVMF IYREEYYLSR KEPAASDPKH AEKYAEWQDN MSKAHNITDI IIAKHRSGPV GNVQLFYDSQ YSKFVNLETK YPVHPR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAC GTAATAAAAC AAACAACGAT AAAAATATAG CTAACGATGA AGCAAGTACT GTATTAGCAA GAGTTTTACC TTCAAATGTT CAAGCAGAAC AAATGTTGCT TGGAGCAATT TTAACTAATA ATGAATTGCT AAATTATGTA TCAGAATTTT TACGCAGTGA GCATTTTTTT GAACCTATTC ACCAAAAAAT CTATAATGGA ATTGAAGTAA TTACCGAGAA AGGTTTAATA GCTACCCCTA TAACATTACG TAGTATGCTA GCAAATGATG AGCTTTTTCA TGAAATGGAG GGAGCTGAAT ATTTAGCAAA ATTAGTCACT ATGTCAATGA TGGTAATTAA TCCTATTGAC TATGGTAAAA TAATATATGA TTTAGCAATA AAACGTTATT TAATAAATAT CGGTGAAGAA GTAGTAAACG ACGCATATAA TTCTTCTTTA GAGTTTGAAG CAAAAGAACA AATTGAACGA GCCGAATCTA AGCTTTATGA TTTAGCTAGT GAAGGGATAA ATGAAAAAAG CTTTACCAAA ATTGCTATAC CCATTTCAGA GTCGATTGCT AGTATAAACA GAGCAATGAA AAATAACGAT CATGTAATAG GTATATCTAC TGGCTTAATT GATTTAGATA GTAAATTATT TGGTTTTCAT AATTCTGATC TTGTAATTCT TGCAGGACGT CCTGCAATGG GTAAAACAGC CTTTGCTTTA AATCTAGCCT TAAATGCTTG CAATAATATG CTTATTAAAA ATAAGCGTGA TAATGAAGAG GTGCAATCTG TGGGATTTTT CTCTCTAGAA ATGTCCTCAG AGCAGCTTAC AACACGTTTG CTTTCAATGA GTGCAGGAAT TGACTCTACT ACTCTTCGTA CGGGAATGAT TAATGAAGCA AATTATAATC GTCTTCGCAA AGAAGCCATA GCTTTATCAG AATTATCATT TTTTATTGAT GATACTCCTG CCTTAACTAT TTCAGCTATA AGAACACGTG CTAGAAGAAT GAAACGCAAG CATAATTTAG GTATATTATT TGTCGATTAT TTACAATTAA TTAGAGGTGT AAGTAAATCC GATAATAGGG TTAATGAAGT TTCAGAAATC ACCCAAGGTT TAAAAGCTAT AGCAAAAGAA TTAAATATTC CTGTCATTGC ATTATCTCAG CTATCAAGAG CCGTAGAGCT TCGTGAGGAT AAAAGACCTA TGCTATCCGA TCTTAGAGAA TCAGGAAGTA TTGAACAAGA TGCCGATATA GTTATGTTTA TTTACCGAGA AGAATATTAT CTAAGCAGAA AAGAGCCAGC AGCAAGCGAT CCTAAACATG CCGAAAAATA TGCCGAATGG CAAGATAATA TGAGTAAAGC ACATAATATT ACCGATATTA TAATTGCAAA ACACCGTAGT GGTCCTGTTG GTAATGTTCA ACTTTTCTAT GACAGTCAAT ATTCTAAATT TGTTAATCTA GAAACAAAAT ATCCAGTGCA TCCGAGATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for RibeA.00169.a.B1.PW36545
PURIFICATION DATe: 7/30/2012
CONCENTRATION: 4.76mg/ml
OBSERVED MW: 56kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 110µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 57
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA RNKTNNDKNI ANDEASTVLA RVLPSNVQAE QMLLGAILTN NELLNYVSEF LRSEHFFEPI HQKIYNGIEV ITEKGLIATP ITLRSMLAND ELFHEMEGAE YLAKLVTMSM MVINPIDYGK IIYDLAIKRY LINIGEEVVN DAYNSSLEFE AKEQIERAES KLYDLASEGI NEKSFTKIAI PISESIASIN RAMKNNDHVI XISTGLIDLD SKLFGFHNSD LVILAGRPAM GKTAFALNLA LNACNNMLIK NKRDNEXVQS XGFFXXEMXS XQLTXVXXX
Validated NT Sequence
tttgtnnnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatggcacg taataaaaca aacaacgata aaaatatagc taacgatgaa gcaagtactg tattagcaag agttttacct tcaaatgttc aagcagaaca aatgttgctt ggagcaattt taactaataa tgaattgcta aattatgtat cagaattttt acgcagtgag catttttttg aacctattca ccaaaaaatc tataatggaa ttgaagtaat taccgagaaa ggtttaatag ctacccctat aacattacgt agtatgctag caaatgatga gctttttcat gaaatggagg gagctgaata tttagcaaaa ttagtcacta tgtcaatgat ggtaattaat cctattgact atggtaaaat aatatatgat ttagcaataa aacgttattt aataaatatc ggtgaagaag tagtaaacga cgcatataat tcttctttag agtttgaagc aaaagaacaa attgaacgag ccgaatctaa gctttatgat ttagctagtg aagggataaa tgaaaaaagc tttaccaaaa ttgctatacc catttcagag tcgattgcta gtataaacag agcaatgaaa aataacgatc atgtaatang tatatctact ggcttaattg atttagatag taaattattt ggntttcata attctgatct tgtaattctt gcaggacgtc ctgcaatggg taaaacagcc tttgctttaa atctagcctt aaatgcttgc aataatatgc ttattaaaaa taagcgtgat aatgaagang tgcaatctgn gggatttttc nctcnngaaa tgncctcana gcagcttaca nncgttngcn ttcnatgann gnngnaattg acnnnactac tnntcgtacg ggnatgnnnn annnncaant nnancgtcnt cnnaanaanc cntncttnnn nnnannnnnc nttttttann gatnnnnnnn ncnnnnncnn antnnngcnn nnanaanncn nnnnnnaann aannnnnnnn gcnnantnnn nnnnnnnncn nnnnntnnnn nnnngnnnnn nannnccnnn nnnnnnnnnn nnnnnannnn nnntnnnnnn nnnnnnnnnn aannnnnng
Expressed Protein Sequence
MAHHHHHHMA RNKTNNDKNI ANDEASTVLA RVLPSNVQAE QMLLGAILTN NELLNYVSEF LRSEHFFEPI HQKIYNGIEV ITEKGLIATP ITLRSMLAND ELFHEMEGAE YLAKLVTMSM MVINPIDYGK IIYDLAIKRY LINIGEEVVN DAYNSSLEFE AKEQIERAES KLYDLASEGI NEKSFTKIAI PISESIASIN RAMKNNDHVI GISTGLIDLD SKLFGFHNSD LVILAGRPAM GKTAFALNLA LNACNNMLIK NKRDNEEVQS VGFFSLEMSS EQLTTRLLSM SAGIDSTTLR TGMINEANYN RLRKEAIALS ELSFFIDDTP ALTISAIRTR ARRMKRKHNL GILFVDYLQL IRGVSKSDNR VNEVSEITQG LKAIAKELNI PVIALSQLSR AVELREDKRP MLSDLRESGS IEQDADIVMF IYREEYYLSR KEPAASDPKH AEKYAEWQDN MSKAHNITDI IIAKHRSGPV GNVQLFYDSQ YSKFVNLETK YPVHPR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAC GTAATAAAAC AAACAACGAT AAAAATATAG CTAACGATGA AGCAAGTACT GTATTAGCAA GAGTTTTACC TTCAAATGTT CAAGCAGAAC AAATGTTGCT TGGAGCAATT TTAACTAATA ATGAATTGCT AAATTATGTA TCAGAATTTT TACGCAGTGA GCATTTTTTT GAACCTATTC ACCAAAAAAT CTATAATGGA ATTGAAGTAA TTACCGAGAA AGGTTTAATA GCTACCCCTA TAACATTACG TAGTATGCTA GCAAATGATG AGCTTTTTCA TGAAATGGAG GGAGCTGAAT ATTTAGCAAA ATTAGTCACT ATGTCAATGA TGGTAATTAA TCCTATTGAC TATGGTAAAA TAATATATGA TTTAGCAATA AAACGTTATT TAATAAATAT CGGTGAAGAA GTAGTAAACG ACGCATATAA TTCTTCTTTA GAGTTTGAAG CAAAAGAACA AATTGAACGA GCCGAATCTA AGCTTTATGA TTTAGCTAGT GAAGGGATAA ATGAAAAAAG CTTTACCAAA ATTGCTATAC CCATTTCAGA GTCGATTGCT AGTATAAACA GAGCAATGAA AAATAACGAT CATGTAATAG GTATATCTAC TGGCTTAATT GATTTAGATA GTAAATTATT TGGTTTTCAT AATTCTGATC TTGTAATTCT TGCAGGACGT CCTGCAATGG GTAAAACAGC CTTTGCTTTA AATCTAGCCT TAAATGCTTG CAATAATATG CTTATTAAAA ATAAGCGTGA TAATGAAGAG GTGCAATCTG TGGGATTTTT CTCTCTAGAA ATGTCCTCAG AGCAGCTTAC AACACGTTTG CTTTCAATGA GTGCAGGAAT TGACTCTACT ACTCTTCGTA CGGGAATGAT TAATGAAGCA AATTATAATC GTCTTCGCAA AGAAGCCATA GCTTTATCAG AATTATCATT TTTTATTGAT GATACTCCTG CCTTAACTAT TTCAGCTATA AGAACACGTG CTAGAAGAAT GAAACGCAAG CATAATTTAG GTATATTATT TGTCGATTAT TTACAATTAA TTAGAGGTGT AAGTAAATCC GATAATAGGG TTAATGAAGT TTCAGAAATC ACCCAAGGTT TAAAAGCTAT AGCAAAAGAA TTAAATATTC CTGTCATTGC ATTATCTCAG CTATCAAGAG CCGTAGAGCT TCGTGAGGAT AAAAGACCTA TGCTATCCGA TCTTAGAGAA TCAGGAAGTA TTGAACAAGA TGCCGATATA GTTATGTTTA TTTACCGAGA AGAATATTAT CTAAGCAGAA AAGAGCCAGC AGCAAGCGAT CCTAAACATG CCGAAAAATA TGCCGAATGG CAAGATAATA TGAGTAAAGC ACATAATATT ACCGATATTA TAATTGCAAA ACACCGTAGT GGTCCTGTTG GTAATGTTCA ACTTTTCTAT GACAGTCAAT ATTCTAAATT TGTTAATCTA GAAACAAAAT ATCCAGTGCA TCCGAGATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT