RibeA.00145.a

Aspartyl-tRNA synthetase

CENTER ID: RibeA.00145.a
ORGANISM: Rickettsia bellii RML369-C
ASSOCIATED DISEASE: Typhus
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.00145.a.B1.GE35580 Full length( RibeA.00145.a ) 1 602
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|336407.7.peg.396
RefSeq: YP_537519.1
UniProt: Q1RJN4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHKYRTHNCN ELKISDVGAE VKLSGWVHRR RDHGNLVFVD LRDHYGITQI VFTDQNPQLM DDASRLRYES VVTVIGKVVA RSEETINNTL PTGHIEVLAG EFIVESAADT LPFVINTEKD APEDSRLKHR FLDLRREKLH NNIMLRSQII SYIRQLMTAR GFTEFQTPIL TASSPEGARD FLVPSRLHPG KFYALPQAPQ QFKQLLMVSG FDRYFQIAPC FRDEDARADR SPGEFYQLDI EMSFVTQEDI FSTIEPVMYE LFTKFTDKKV SEAPFIRIPY NESMLKYGSD KPDLRNPIVI ADVTEIFRDS DFTIFRENIK KGSIVRAIPA PYAAAQPRSF FDKMIEFAIS EGAGGLGYIQ FSETGEAKGP VAKFLSTQQL EDLKATANIS NGDAVFFASD KKDKAAKLAG KVRIKLADEL DLLEKDCFKF CWITDFPFYE LNEETGKIDF SHNPFSMPQG GLEALENAKT TEELLELTAY QYDIVCNGIE LSSGAVRNHK PEIMYKAFAI AGYSEEEVDK RFGGMIRAFK FGAPPHGGIA PGIDRIVMLL AEATNIREII AFPLNQQAED LLMNAPNYVE DKALKELNVM LSPSARKNAE KE
NT Sequence
ATGCATAAAT ATAGAACTCA TAATTGTAAC GAATTAAAAA TTTCTGATGT TGGAGCTGAA GTAAAATTAT CTGGTTGGGT ACATAGAAGA AGAGATCATG GCAATCTAGT TTTTGTAGAT TTACGTGATC ATTATGGCAT AACACAAATC GTGTTTACCG ACCAAAATCC GCAGCTTATG GACGATGCTA GCCGTTTACG TTATGAGTCA GTTGTTACAG TAATTGGAAA AGTAGTAGCA AGATCAGAAG AGACTATTAA TAATACGCTT CCTACCGGTC ATATAGAAGT TTTAGCCGGT GAGTTTATTG TTGAATCAGC TGCTGATACT TTACCATTTG TGATTAATAC GGAAAAAGAC GCACCTGAAG ACTCAAGGCT TAAACATCGC TTTTTAGATT TAAGACGTGA GAAGTTGCAT AATAATATAA TGCTACGTTC ACAAATTATT TCTTATATCC GTCAATTGAT GACTGCAAGA GGTTTTACCG AGTTCCAAAC ACCTATTTTA ACTGCTAGCT CACCGGAAGG AGCGAGGGAT TTCTTAGTCC CAAGCAGATT ACATCCGGGC AAATTTTATG CATTACCGCA AGCACCGCAA CAATTCAAGC AACTATTAAT GGTATCGGGA TTTGATCGTT ATTTCCAAAT TGCTCCGTGT TTTCGTGATG AAGACGCAAG AGCAGATAGG TCACCAGGCG AGTTTTATCA GCTAGATATC GAAATGTCGT TTGTTACACA AGAAGATATA TTCAGCACAA TCGAGCCTGT AATGTATGAG CTATTTACGA AATTTACAGA TAAGAAAGTA TCAGAAGCAC CCTTTATCCG TATTCCCTAT AATGAATCTA TGCTGAAATA TGGTTCTGAT AAGCCTGATT TACGAAACCC TATTGTAATT GCTGATGTAA CGGAAATTTT TAGAGATTCA GACTTTACTA TTTTTAGGGA AAATATCAAA AAAGGTAGTA TTGTCCGAGC AATTCCAGCT CCTTATGCAG CCGCACAACC TCGTAGTTTC TTTGATAAAA TGATAGAGTT TGCAATATCG GAGGGAGCTG GAGGACTTGG CTATATTCAG TTTAGCGAAA CCGGTGAAGC AAAAGGACCA GTTGCAAAAT TCTTAAGCAC TCAGCAGTTA GAAGATTTAA AGGCTACTGC TAATATTAGC AATGGCGATG CGGTATTTTT CGCCAGCGAT AAAAAAGATA AAGCTGCAAA ACTTGCGGGT AAGGTAAGAA TTAAGCTTGC CGATGAGTTA GATTTACTTG AAAAGGATTG CTTTAAATTC TGCTGGATTA CCGATTTTCC ATTCTATGAG CTAAACGAAG AAACCGGTAA AATTGATTTT AGCCATAATC CGTTTTCTAT GCCTCAGGGT GGGTTAGAAG CTCTTGAGAA CGCAAAAACA ACCGAAGAGT TACTAGAGCT TACCGCATAT CAGTATGATA TTGTTTGTAA TGGCATAGAA CTTTCTAGCG GTGCTGTTAG AAATCATAAG CCTGAGATAA TGTATAAGGC ATTTGCTATA GCTGGTTACA GTGAAGAGGA AGTTGATAAG AGATTCGGCG GTATGATCAG AGCGTTTAAA TTTGGTGCCC CGCCACATGG CGGCATAGCC CCAGGTATAG ACCGAATCGT TATGTTACTT GCGGAAGCTA CTAATATAAG GGAGATAATC GCTTTTCCAT TAAATCAGCA AGCTGAAGAT CTATTAATGA ACGCCCCGAA TTACGTAGAA GATAAGGCGT TAAAAGAATT AAATGTTATG CTCTCTCCAT CTGCTAGAAA GAATGCGGAG AAAGAG
Details for RibeA.00145.a.B1.GE35580
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/14/2012
EXPECTED MW: 69kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 36
Validated AA Sequence
MAHHHHHHMH KYRTHNCNEL KISDVGAEVK LSGWVHRRRD HGNLVFVDLR DHYGITQIVF TDQNPQLMDD ASRLRYESVV TVIGKVVARS EETINNTLPT GHIEVLAGEF IVESAADTLP FVINTEKDAP EDSRLKHRFL DLRREKLHNN IMLRSQIISY IRQLMTARGF TEFQTPILTA SSPEGARDFL VPSRLHPGKF YALPQAPXQF KQLLMVSGFD RYF
Validated NT Sequence
atggctcacc accaccacca ccatatgcat aaatatagaa ctcataattg taacgaatta aaaatttctg atgttggagc tgaagtaaaa ttatctggtt gggtacatag aagaagagat catggcaatc tagtttttgt agatttacgt gatcattatg gcataacaca aatcgtgttt accgaccaaa atccgcagct tatggacgat gctagccgtt tacgttatga gtcagttgtt acagtaattg gaaaagtagt agcaagatca gaagagacta ttaataatac gcttcctacc ggtcatatag aagttttagc cggtgagttt attgttgaat cagctgctga tactttacca tttgtgatta atacggaaaa agacgcacct gaagactcaa ggcttaaaca tcgcttttta gatttaagac gtgagaagtt gcataataat ataatgctac gttcacaaat tatttcttat atccgtcaat tgatgactgc aagaggtttt accgagttcc aaacacctat tttaactgct agctcaccgg aaggagcgag ggatttctta gtcccaagca gattacatcc gggcaaattt tatgcattac cgcaagcacc gcnncaattc aagcaactat taatggtatc gggatttgat cgttatttcc
Expected Protein Sequence
MAHHHHHHMH KYRTHNCNEL KISDVGAEVK LSGWVHRRRD HGNLVFVDLR DHYGITQIVF TDQNPQLMDD ASRLRYESVV TVIGKVVARS EETINNTLPT GHIEVLAGEF IVESAADTLP FVINTEKDAP EDSRLKHRFL DLRREKLHNN IMLRSQIISY IRQLMTARGF TEFQTPILTA SSPEGARDFL VPSRLHPGKF YALPQAPQQF KQLLMVSGFD RYFQIAPCFR DEDARADRSP GEFYQLDIEM SFVTQEDIFS TIEPVMYELF TKFTDKKVSE APFIRIPYNE SMLKYGSDKP DLRNPIVIAD VTEIFRDSDF TIFRENIKKG SIVRAIPAPY AAAQPRSFFD KMIEFAISEG AGGLGYIQFS ETGEAKGPVA KFLSTQQLED LKATANISNG DAVFFASDKK DKAAKLAGKV RIKLADELDL LEKDCFKFCW ITDFPFYELN EETGKIDFSH NPFSMPQGGL EALENAKTTE ELLELTAYQY DIVCNGIELS SGAVRNHKPE IMYKAFAIAG YSEEEVDKRF GGMIRAFKFG APPHGGIAPG IDRIVMLLAE ATNIREIIAF PLNQQAEDLL MNAPNYVEDK ALKELNVMLS PSARKNAEKE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcata aatatagaac tcataattgt aacgaattaa aaatttctga tgttggagct gaagtaaaat tatctggttg ggtacataga agaagagatc atggcaatct agtttttgta gatttacgtg atcattatgg cataacacaa atcgtgttta ccgaccaaaa tccgcagctt atggacgatg ctagccgttt acgttatgag tcagttgtta cagtaattgg aaaagtagta gcaagatcag aagagactat taataatacg cttcctaccg gtcatataga agttttagcc ggtgagttta ttgttgaatc agctgctgat actttaccat ttgtgattaa tacggaaaaa gacgcacctg aagactcaag gcttaaacat cgctttttag atttaagacg tgagaagttg cataataata taatgctacg ttcacaaatt atttcttata tccgtcaatt gatgactgca agaggtttta ccgagttcca aacacctatt ttaactgcta gctcaccgga aggagcgagg gatttcttag tcccaagcag attacatccg ggcaaatttt atgcattacc gcaagcaccg caacaattca agcaactatt aatggtatcg ggatttgatc gttatttcca aattgctccg tgttttcgtg atgaagacgc aagagcagat aggtcaccag gcgagtttta tcagctagat atcgaaatgt cgtttgttac acaagaagat atattcagca caatcgagcc tgtaatgtat gagctattta cgaaatttac agataagaaa gtatcagaag caccctttat ccgtattccc tataatgaat ctatgctgaa atatggttct gataagcctg atttacgaaa ccctattgta attgctgatg taacggaaat ttttagagat tcagacttta ctatttttag ggaaaatatc aaaaaaggta gtattgtccg agcaattcca gctccttatg cagccgcaca acctcgtagt ttctttgata aaatgataga gtttgcaata tcggagggag ctggaggact tggctatatt cagtttagcg aaaccggtga agcaaaagga ccagttgcaa aattcttaag cactcagcag ttagaagatt taaaggctac tgctaatatt agcaatggcg atgcggtatt tttcgccagc gataaaaaag ataaagctgc aaaacttgcg ggtaaggtaa gaattaagct tgccgatgag ttagatttac ttgaaaagga ttgctttaaa ttctgctgga ttaccgattt tccattctat gagctaaacg aagaaaccgg taaaattgat tttagccata atccgttttc tatgcctcag ggtgggttag aagctcttga gaacgcaaaa acaaccgaag agttactaga gcttaccgca tatcagtatg atattgtttg taatggcata gaactttcta gcggtgctgt tagaaatcat aagcctgaga taatgtataa ggcatttgct atagctggtt acagtgaaga ggaagttgat aagagattcg gcggtatgat cagagcgttt aaatttggtg ccccgccaca tggcggcata gccccaggta tagaccgaat cgttatgtta cttgcggaag ctactaatat aagggagata atcgcttttc cattaaatca gcaagctgaa gatctattaa tgaacgcccc gaattacgta gaagataagg cgttaaaaga attaaatgtt atgctctctc catctgctag aaagaatgcg gagaaagagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat