SSGCID: Target Page

RibeA.00141.a

Malonyl CoA-acyl carrier protein transacylase

CENTER ID:	RibeA.00141.a
ORGANISM:	Rickettsia bellii RML369-C
ASSOCIATED DISEASE:	Typhus
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RibeA.00141.a.B1.GE35577	Full length( RibeA.00141.a )		1	312

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RibeA.00141.a.B1.PS01594	Full length( RibeA.00141.a )		1	312

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|336407.7.peg.768
RefSeq:	YP_537841.1
UniProt:	Q1RIR2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKTAFIFPGQ GSQAIGMGND FYDNFKSAKE TFQTIDEALN RKLTDIIFNS TSEELTLTTN AQPALMAVSM AIINTIKAET NKNIVQLCDY VAGHSLGEYS ALCAAESISI ETAAKLLHTR SSSMQEACPA NFGAMAACIG ISIDKLEKIV KESKCEIAND NIEGQIVISG KVEAVDHAIN LIKEAGYKAI KLKVSAPFHC SLMKPAEEKM RIALDNSVIT KPLIPVIQNY TAKPVLEPLE IKQNLVLQIC GRVRWRETLE LFNDLNITHI VEIGAGNVLT NMLGKINYPF KLSNISNVEE MKLFLQAYET AN

NT Sequence

ATGAAAACAG CCTTTATCTT TCCAGGTCAA GGGTCGCAAG CCATTGGCAT GGGCAATGAT TTTTACGATA ATTTTAAATC CGCAAAAGAA ACATTCCAAA CAATCGATGA AGCACTAAAC CGAAAACTTA CTGATATAAT TTTTAATTCT ACTTCGGAAG AATTAACATT AACTACTAAT GCACAGCCAG CATTAATGGC TGTATCTATG GCGATAATAA ATACCATAAA AGCCGAGACT AATAAAAATA TAGTTCAGCT TTGTGATTAT GTTGCTGGTC ATTCCTTAGG TGAATACTCT GCTCTATGTG CTGCTGAAAG TATTAGCATT GAGACGGCAG CGAAATTACT GCATACACGC AGCAGCTCCA TGCAAGAAGC CTGCCCTGCT AATTTTGGTG CTATGGCTGC TTGTATCGGT ATATCCATCG ATAAGCTTGA GAAAATAGTT AAAGAAAGCA AATGCGAAAT AGCAAATGAT AATATCGAAG GACAGATTGT AATTAGCGGT AAAGTGGAAG CTGTTGATCA CGCTATTAAC CTTATTAAAG AAGCAGGCTA TAAAGCAATA AAGCTAAAGG TAAGTGCTCC GTTTCACTGT AGTTTAATGA AGCCGGCAGA GGAGAAAATG CGAATAGCAC TTGATAATAG CGTAATTACC AAACCGCTAA TACCAGTAAT TCAAAATTAT ACTGCAAAGC CTGTGTTAGA ACCTCTAGAA ATCAAGCAAA ATCTAGTTTT ACAAATATGC GGACGAGTTA GATGGCGTGA AACTTTAGAG CTATTTAACG ATCTAAATAT CACCCATATA GTAGAAATAG GAGCAGGAAA TGTGCTAACA AATATGCTTG GAAAAATCAA CTATCCATTT AAATTGAGTA ATATAAGTAA CGTAGAAGAG ATGAAACTAT TTCTACAAGC TTATGAAACT GCAAAT

Details for RibeA.00141.a.B1.GE35577

HARVESTED ON:	6/12/2012
SEQUENCED ON:	6/12/2012
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

tactcactaa tttgcagttt cataagcttg tagaaatagt ttcatctctt ctacgttact tatattactc aatttaaatg gatagttgat ttttccaagc atatttgtta gcacatttcc tgctcctatt tctactatat gggtgatatt tagatcgtta aatagctcta aagtttcacg ccatctaact cgtccgcata tttgtaaaac tagattttgc ttgatttcta gaggttctaa cacaggcttt gcagtataat tttgaattac tggtattagc ggtttggtaa ttacgctatt atcaagtgct attcgcattt tctcctctgc cggcttcatt aaactacagt gaaacggagc acttaccttt agctttattg ctttatagcc tgcttcttta ataaggttaa tagcgtgatc aacagcttcc actttaccgc taattacaat ctgtccttcg atattatcat ttgctatttc gcatttgctt tctttaacta ttttctcaag cttatcgatg gatataccga tacaagcagc catagcacca aaattagcag ggcaggcttc ttgcatggag ctgctgcgtg tatgcagtaa tttcgctgcc gtctcaatgc taatactttc agcagcacat agagcagagt attcacctaa ggaatgacca gcaacataat cacaaagctg aactatattt ttattagtct cggcttttat ggtatttatt atcgccatag atacagccat taatgctggc tgtgcattag tagttaatgt taattcttcc gaagtagaat taaaaattat atcagtaagt tttcggttta gtgcttcatc gattgtttgg aatgtttctt ttgcggattt aaaattatcg taaaaatcat tgcccatgcc aatggcttgc gacccttgac ctggaaagat aaaggctgtt ttcatatg

Expected Protein Sequence

MAHHHHHHMK TAFIFPGQGS QAIGMGNDFY DNFKSAKETF QTIDEALNRK LTDIIFNSTS EELTLTTNAQ PALMAVSMAI INTIKAETNK NIVQLCDYVA GHSLGEYSAL CAAESISIET AAKLLHTRSS SMQEACPANF GAMAACIGIS IDKLEKIVKE SKCEIANDNI EGQIVISGKV EAVDHAINLI KEAGYKAIKL KVSAPFHCSL MKPAEEKMRI ALDNSVITKP LIPVIQNYTA KPVLEPLEIK QNLVLQICGR VRWRETLELF NDLNITHIVE IGAGNVLTNM LGKINYPFKL SNISNVEEMK LFLQAYETAN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa cagcctttat ctttccaggt caagggtcgc aagccattgg catgggcaat gatttttacg ataattttaa atccgcaaaa gaaacattcc aaacaatcga tgaagcacta aaccgaaaac ttactgatat aatttttaat tctacttcgg aagaattaac attaactact aatgcacagc cagcattaat ggctgtatct atggcgataa taaataccat aaaagccgag actaataaaa atatagttca gctttgtgat tatgttgctg gtcattcctt aggtgaatac tctgctctat gtgctgctga aagtattagc attgagacgg cagcgaaatt actgcataca cgcagcagct ccatgcaaga agcctgccct gctaattttg gtgctatggc tgcttgtatc ggtatatcca tcgataagct tgagaaaata gttaaagaaa gcaaatgcga aatagcaaat gataatatcg aaggacagat tgtaattagc ggtaaagtgg aagctgttga tcacgctatt aaccttatta aagaagcagg ctataaagca ataaagctaa aggtaagtgc tccgtttcac tgtagtttaa tgaagccggc agaggagaaa atgcgaatag cacttgataa tagcgtaatt accaaaccgc taataccagt aattcaaaat tatactgcaa agcctgtgtt agaacctcta gaaatcaagc aaaatctagt tttacaaata tgcggacgag ttagatggcg tgaaacttta gagctattta acgatctaaa tatcacccat atagtagaaa taggagcagg aaatgtgcta acaaatatgc ttggaaaaat caactatcca tttaaattga gtaatataag taacgtagaa gagatgaaac tatttctaca agcttatgaa actgcaaatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for RibeA.00141.a.B1.PS01594

PURIFICATION DATe:	8/14/2012
CONCENTRATION:	100.44mg/ml
OBSERVED MW:	31kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	9
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAA CAGCCTTTAT CTTTCCAGGT CAAGGGTCGC AAGCCATTGG CATGGGCAAT GATTTTTACG ATAATTTTAA ATCCGCAAAA GAAACATTCC AAACAATCGA TGAAGCACTA AACCGAAAAC TTACTGATAT AATTTTTAAT TCTACTTCGG AAGAATTAAC ATTAACTACT AATGCACAGC CAGCATTAAT GGCTGTATCT ATGGCGATAA TAAATACCAT AAAAGCCGAG ACTAATAAAA ATATAGTTCA GCTTTGTGAT TATGTTGCTG GTCATTCCTT AGGTGAATAC TCTGCTCTAT GTGCTGCTGA AAGTATTAGC ATTGAGACGG CAGCGAAATT ACTGCATACA CGCAGCAGCT CCATGCAAGA AGCCTGCCCT GCTAATTTTG GTGCTATGGC TGCTTGTATC GGTATATCCA TCGATAAGCT TGAGAAAATA GTTAAAGAAA GCAAATGCGA AATAGCAAAT GATAATATCG AAGGACAGAT TGTAATTAGC GGTAAAGTGG AAGCTGTTGA TCACGCTATT AACCTTATTA AAGAAGCAGG CTATAAAGCA ATAAAGCTAA AGGTAAGTGC TCCGTTTCAC TGTAGTTTAA TGAAGCCGGC AGAGGAGAAA ATGCGAATAG CACTTGATAA TAGCGTAATT ACCAAACCGC TAATACCAGT AATTCAAAAT TATACTGCAA AGCCTGTGTT AGAACCTCTA GAAATCAAGC AAAATCTAGT TTTACAAATA TGCGGACGAG TTAGATGGCG TGAAACTTTA GAGCTATTTA ACGATCTAAA TATCACCCAT ATAGTAGAAA TAGGAGCAGG AAATGTGCTA ACAAATATGC TTGGAAAAAT CAACTATCCA TTTAAATTGA GTAATATAAG TAACGTAGAA GAGATGAAAC TATTTCTACA AGCTTATGAA ACTGCAAATT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT