SSGCID: Target Page

RibeA.00137.a

UDP-N-acetylmuramate--L-alanine ligase

CENTER ID:	RibeA.00137.a
ORGANISM:	Rickettsia bellii RML369-C
ASSOCIATED DISEASE:	Typhus
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RibeA.00137.a.B1.GE35572	Full length( RibeA.00137.a )		1	478

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|336407.7.peg.1093
RefSeq:	YP_538133.1
UniProt:	Q1RHX0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLLLELKKTN QTLETIHFIG IGGVGMSGIA EILHNLGYKV QGSDLSENYN TKRLEAYGIK IFVGHTPQNI TNVSYVVVSS AINRNNPEVK EALERKIPII RRAEMLAELM RLKCSVAVSG SHGKTTTTSL VACLFEAAGL HPTVINGGII NNRSTNAYLG SSNYLIAEAD ESDATFISIP STIAIITNID PEHLDYYKDF DTLINAFRSF ITNLPFYGFA VCCIDHKIVR ELVNNITERK VITYGIDSDD AHITAFNIST DITSSTFDVK ISLPNVSGVT IIEKITIPTP GRHNILNSLA AISVGVELDF GIKAIKNGFN SFKGVKRRFT KVAEYNQASV IDDYAHHPEE IKATLSTAKN IANKQHGKVI AIFQPHRYSR MQHLFDDFMR CFDDADLLYI TDVYAAGESP IEGISGQSLI DNIIKLKYHN QANFLAKLDD SLEVIMNKAS PGDMIIMMGA GNISNFANEL PQKFGNLS

NT Sequence

ATGCTGTTAC TTGAATTAAA AAAAACTAAT CAAACTTTAG AAACTATACA TTTTATTGGC ATTGGCGGCG TTGGAATGAG CGGCATTGCT GAGATATTAC ATAATCTTGG TTATAAGGTG CAAGGTTCTG ATTTATCAGA AAATTATAAT ACCAAACGTT TGGAAGCATA TGGCATTAAA ATATTTGTTG GTCATACACC ACAAAATATT ACAAATGTCT CATATGTTGT TGTTTCTTCG GCAATTAATC GAAATAATCC GGAAGTAAAA GAAGCTTTGG AGCGTAAAAT CCCAATCATC AGACGGGCAG AAATGCTTGC TGAACTTATG AGGTTAAAAT GCTCGGTAGC GGTATCTGGT TCGCATGGTA AGACTACTAC CACTTCTTTA GTTGCTTGCT TATTTGAGGC AGCTGGTTTA CATCCTACAG TCATTAATGG TGGTATTATT AATAATAGGT CAACAAATGC TTATCTTGGT TCAAGTAATT ATTTAATTGC TGAAGCAGAT GAGTCAGATG CAACTTTTAT CAGTATTCCG TCGACTATTG CAATAATAAC CAATATTGAT CCGGAACATT TAGATTATTA CAAAGATTTT GATACATTAA TTAATGCCTT TAGGAGTTTC ATTACAAATT TACCTTTTTA TGGTTTTGCC GTTTGCTGTA TTGACCATAA AATAGTACGT GAGTTAGTAA ATAATATTAC CGAGCGGAAA GTTATCACTT ATGGTATTGA TTCCGATGAT GCTCATATTA CCGCATTTAA TATTAGTACA GATATTACTT CATCTACCTT TGATGTAAAA ATTAGTTTGC CAAATGTAAG TGGTGTTACA ATTATAGAAA AAATTACTAT TCCTACTCCA GGAAGGCATA ATATTCTAAA TAGTTTAGCA GCAATTAGCG TAGGGGTGGA GTTAGATTTC GGTATTAAGG CTATTAAAAA CGGCTTTAAT AGCTTTAAAG GAGTAAAAAG AAGATTTACT AAAGTAGCTG AATATAATCA AGCTTCGGTT ATTGATGATT ATGCCCATCA CCCTGAAGAA ATTAAGGCAA CATTATCTAC TGCTAAAAAC ATAGCCAATA AGCAACATGG TAAGGTTATT GCTATTTTCC AGCCGCATAG ATATTCAAGA ATGCAGCATT TATTTGATGA TTTTATGCGT TGCTTTGATG ATGCGGATTT ACTTTATATT ACAGATGTAT ATGCGGCAGG TGAAAGCCCT ATAGAGGGTA TTAGCGGGCA AAGTTTAATT GATAACATTA TTAAGCTCAA ATATCATAAT CAAGCAAATT TCTTAGCAAA ATTAGATGAT AGCTTAGAAG TTATAATGAA TAAAGCGTCT CCTGGTGATA TGATCATTAT GATGGGAGCA GGTAATATTT CAAACTTTGC TAATGAATTA CCGCAAAAAT TTGGTAATTT GTCA

Details for RibeA.00137.a.B1.GE35572

HARVESTED ON:	6/12/2012
SEQUENCED ON:	6/12/2012
EXPECTED MW:	53kDa
OBSERVED MW:	53kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	45

Validated AA Sequence

MSGIAEILHN LGYKVQGSDL SENYNTKRLE AYGIKIFVGH TPQNITNVSY VVVSSAINRN NPEVKEALER KIPIIRRAEM LAELMRLKCS VAVSGSHGKT TTTSLVACLF EAAGLHPTVI NGGIINNRST NAYLGSSNYL IAEADESDAT FISIPSTIAI ITNIDPEHLD YYKDFDTLIN AFRSFITNLP FYGFAVCCID HKIVRELVNN XYRAESYHLW Y

Validated NT Sequence

taattcatta gcaaagtttg aaatattacc tgctcccatc ataatgatca tatcaccagg agacgcttta ttcattataa cttctaagct atcatctaat tttgctaaga aatttgcttg attatgatat ttgagcttaa taatgttatc aattaaactt tgcccgctaa taccctctat agggctttca cctgccgcat atacatctgt aatataaagt aaatccgcat catcaaagca acgcataaaa tcatcaaata aatgctgcat tcttgaatat ctatgcggct ggaaaatagc aataacctta ccatgttgct tattggctat gtttttagca gtagataatg ttgccttaat ttcttcaggg tgatgggcat aatcatcaat aaccgaagct tgattatatt cagctacttt agtaaatctt ctttttactc ctttaaagct attaaagccg tttttaatag ccttaatacc gaaatctaac tccaccccta cgctaattgc tgctaaacta tttagaatat tatgccttcc tggagtagga atagtaattt tttctataat tgtaacacca cttacatttg gcaaactaat ttttacatca naggtagatg aagtaatatc tgtactaata ttaaatgcgg taatatgagc atcatcggaa tcaataccat aagtgataac tttccgctcg gtaantatta tttactaact cacgtactat tttatggtca atacagcaaa cggcaaaacc ataaaaaggt aaatttgtaa tgaaactcct aaaggcatta attaatgtat caaaatcttt gtaataatct aaatgttccg gatcaatatt ggttattatt gcaatagtcg acggaatact gataaaagtt gcatctgact catctgcttc agcaattaaa taattacttg aaccaagata agcatttgtt gacctattat taataatacc accattaatg actgtaggat gtaaaccagc tgcctcaaat aagcaagcaa ctaaagaagt ggtagtagtc ttaccatgcg aaccagatac cgctaccgag cattttaacc tcataagttc agcaagcatt tctgcccgtc tgatgattgg gattttacgc tccaaagctt cttttacttc cggattattt cgattaattg ccgaagaaac aacaacatat gagacatttg taatattttg tggtgtatga ccaacaaata ttttaatgcc atatgcttcc aaacgtttgg tattataatt ttctgataaa tcagaacctt gcaccttata accaagatta tgtaatatct cagcaatgcc gctcattcca acgccgccaa tgccaataaa atgtatagtt tctaaagttt gattagtttt ttttaattca agtaacagca

Expected Protein Sequence

MAHHHHHHML LLELKKTNQT LETIHFIGIG GVGMSGIAEI LHNLGYKVQG SDLSENYNTK RLEAYGIKIF VGHTPQNITN VSYVVVSSAI NRNNPEVKEA LERKIPIIRR AEMLAELMRL KCSVAVSGSH GKTTTTSLVA CLFEAAGLHP TVINGGIINN RSTNAYLGSS NYLIAEADES DATFISIPST IAIITNIDPE HLDYYKDFDT LINAFRSFIT NLPFYGFAVC CIDHKIVREL VNNITERKVI TYGIDSDDAH ITAFNISTDI TSSTFDVKIS LPNVSGVTII EKITIPTPGR HNILNSLAAI SVGVELDFGI KAIKNGFNSF KGVKRRFTKV AEYNQASVID DYAHHPEEIK ATLSTAKNIA NKQHGKVIAI FQPHRYSRMQ HLFDDFMRCF DDADLLYITD VYAAGESPIE GISGQSLIDN IIKLKYHNQA NFLAKLDDSL EVIMNKASPG DMIIMMGAGN ISNFANELPQ KFGNLS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgt tacttgaatt aaaaaaaact aatcaaactt tagaaactat acattttatt ggcattggcg gcgttggaat gagcggcatt gctgagatat tacataatct tggttataag gtgcaaggtt ctgatttatc agaaaattat aataccaaac gtttggaagc atatggcatt aaaatatttg ttggtcatac accacaaaat attacaaatg tctcatatgt tgttgtttct tcggcaatta atcgaaataa tccggaagta aaagaagctt tggagcgtaa aatcccaatc atcagacggg cagaaatgct tgctgaactt atgaggttaa aatgctcggt agcggtatct ggttcgcatg gtaagactac taccacttct ttagttgctt gcttatttga ggcagctggt ttacatccta cagtcattaa tggtggtatt attaataata ggtcaacaaa tgcttatctt ggttcaagta attatttaat tgctgaagca gatgagtcag atgcaacttt tatcagtatt ccgtcgacta ttgcaataat aaccaatatt gatccggaac atttagatta ttacaaagat tttgatacat taattaatgc ctttaggagt ttcattacaa atttaccttt ttatggtttt gccgtttgct gtattgacca taaaatagta cgtgagttag taaataatat taccgagcgg aaagttatca cttatggtat tgattccgat gatgctcata ttaccgcatt taatattagt acagatatta cttcatctac ctttgatgta aaaattagtt tgccaaatgt aagtggtgtt acaattatag aaaaaattac tattcctact ccaggaaggc ataatattct aaatagttta gcagcaatta gcgtaggggt ggagttagat ttcggtatta aggctattaa aaacggcttt aatagcttta aaggagtaaa aagaagattt actaaagtag ctgaatataa tcaagcttcg gttattgatg attatgccca tcaccctgaa gaaattaagg caacattatc tactgctaaa aacatagcca ataagcaaca tggtaaggtt attgctattt tccagccgca tagatattca agaatgcagc atttatttga tgattttatg cgttgctttg atgatgcgga tttactttat attacagatg tatatgcggc aggtgaaagc cctatagagg gtattagcgg gcaaagttta attgataaca ttattaagct caaatatcat aatcaagcaa atttcttagc aaaattagat gatagcttag aagttataat gaataaagcg tctcctggtg atatgatcat tatgatggga gcaggtaata tttcaaactt tgctaatgaa ttaccgcaaa aatttggtaa tttgtcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at