SSGCID: Target Page

RibeA.00135.a

Queuine tRNA-ribosyltransferase

CENTER ID:	RibeA.00135.a
ORGANISM:	Rickettsia bellii RML369-C
ASSOCIATED DISEASE:	Typhus
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RibeA.00135.a.B1.GE35573	Full length( RibeA.00135.a )		1	359

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|336407.7.peg.1421
RefSeq:	YP_538428.1
UniProt:	Q1RH25

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKFSFNINH QYKKARSGII TTAHGNIRTP AFMPVGTRGT VKAMLPESVA ETGADILLGN TYHLMLQPSA ERIAKLGGLH KFMNWGKPIL TDSGGFQVMS LSKLRKITEE GVSFNSHING DKYLLTPERS TEIQHLLGST ITMAFDECTP YPATFEEAKT SMQLTTRWAH RSREAFVKRD GYAQFGIIQG STYEELREQS AKDLIELDFE GYAIGGLAVG EGQELMFKVL APDFLPQNKP RYLMGVGKPA DIIGAVSRGV DMFDCVIPTR SGRNGQAFTK YGTVNIRNSK YAEDNDPLEA DCLCPACQNY SKAYLHHLVR IGEILGAMLM TWHNLTYFQN LMSRIREYIA LGKDFDFTT

NT Sequence

GTGTCAAAAT TTTCCTTTAA TATCAATCAT CAATATAAAA AAGCAAGAAG CGGGATTATT ACTACTGCTC ACGGCAATAT TCGTACGCCT GCTTTTATGC CGGTCGGAAC AAGAGGAACT GTTAAAGCAA TGCTGCCTGA ATCGGTAGCC GAAACAGGGG CTGATATTCT GCTTGGTAAC ACCTACCACT TAATGCTACA ACCAAGTGCC GAGCGTATTG CTAAGCTTGG TGGTTTGCAT AAATTTATGA ATTGGGGTAA GCCAATACTA ACCGATTCTG GTGGTTTTCA AGTAATGTCT TTATCAAAAT TACGCAAGAT AACGGAAGAG GGGGTAAGTT TTAACTCGCA TATTAATGGT GATAAATATT TGCTGACGCC TGAACGTTCT ACCGAAATAC AACATCTACT CGGCAGCACT ATTACCATGG CTTTTGACGA ATGTACGCCT TACCCTGCAA CGTTTGAAGA AGCAAAAACC TCTATGCAGC TCACAACTAG ATGGGCACAT AGATCAAGAG AAGCGTTTGT TAAGCGAGAT GGTTATGCAC AGTTTGGTAT TATTCAAGGT AGCACTTACG AAGAGTTACG TGAGCAATCG GCTAAAGATT TAATAGAGCT AGATTTTGAA GGATATGCTA TAGGCGGTCT TGCAGTTGGG GAGGGGCAAG AACTTATGTT TAAAGTTCTT GCCCCTGATT TCCTACCGCA AAATAAACCT AGATATTTGA TGGGGGTAGG AAAGCCTGCT GATATTATCG GAGCAGTCAG CAGAGGGGTA GATATGTTTG ACTGCGTAAT CCCGACTCGG TCAGGTCGTA ATGGTCAGGC TTTTACAAAA TACGGCACGG TAAATATCCG CAATAGTAAA TATGCTGAAG ATAACGATCC TTTGGAAGCT GATTGCCTTT GCCCTGCTTG TCAAAATTAT AGCAAAGCCT ATTTACATCA CTTGGTGAGA ATAGGCGAAA TACTTGGAGC GATGCTAATG ACTTGGCACA ATCTTACATA TTTCCAAAAC CTTATGAGCC GTATTAGAGA GTATATTGCC TTAGGTAAAG ATTTTGATTT TACTACT

Details for RibeA.00135.a.B1.GE35573

HARVESTED ON:	6/12/2012
SEQUENCED ON:	6/12/2012
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	70

Validated AA Sequence

MAHHHHHHMV SKFSFNINHQ YKKARSGIIT TAHGNIRTPA FMPVGTRGTV KAMLPESVAE TGADILLGNT YHLMLQPSAE RIAKLGGLHK FMNWGKPILT DSGGFQVMSL SKLRKITEEG VSFNSHINGD KYLLTPERST EIQHLLGSTI TMAFDECTPY PATFEEAKTS MQLTTRWAHR SREAFVKRDG YAQFGIIQGS TYEELREQSA KDLIELDFEG YAIGGLAVGE GQELMFKVLA XDFLPQINLX XXGGRKAC

Validated NT Sequence

ttnntnnntt taagaaggag anataccatg gctcaccacc accaccacca tatggtgtca aaattttcct ttaatatcaa tcatcaatat aaaaaagcaa gaagcgggat tattactact gctcacggca atattcgtac gcctgctttt atgccggtcg gaacaagagg aactgttaaa gcaatgctgc ctgaatcggt agccgaaaca ggggctgata ttctgcttgg taacacctac cacttaatgc tacaaccaag tgccgagcgt attgctaagc ttggtggttt gcataaattt atgaattggg gtaagccaat actaaccgat tctggtggtt ttcaagtaat gtctttatca aaattacgca agataacgga agagggggta agttttaact cgcatattaa tggtgataaa tatttgctga cgcctgaacg ttctaccgaa atacaacatc tactcggcag cactattacc atggcttttg acgaatgtac gccttaccct gcaacgtttg aagaagcaaa aacctctatg cagctcacaa ctagatgggc acatagatca agagaagcgt ttgttaagcg agatggttat gcacagtttg gtattattca aggtagcact tacgaagagt tacgtgagca atcggctaaa gatttaatag agctagattt tgaaggatat gctataggcg gtcttgcagt tggggagggg caagaactta tgtttaaagt tcttgccncn gatttcctac cgcaaataaa cctanatatn nangggggna ggaaagcctg ctgatnttat cnnancannc ancananggg gnnnanatgt ttgactgcnt aatccnactc ggtcnggncg naatggncnn gctttncaan nnnnnnncgg nnaananccn cannnntaag ntgaannnna cnancnttnn nanctgatng nnttnnccng nntgnnnaan nnnnnnannn cnnntnnnnt nnnnngnnnn nannnnnnnn nannnntgnn ngnnncnnan gnacnngnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntnn nnnnnnncnn nnnnaannnn nnnttcnnnn nnnnnnnnnn nnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgt caaaattttc ctttaatatc aatcatcaat ataaaaaagc aagaagcggg attattacta ctgctcacgg caatattcgt acgcctgctt ttatgccggt cggaacaaga ggaactgtta aagcaatgct gcctgaatcg gtagccgaaa caggggctga tattctgctt ggtaacacct accacttaat gctacaacca agtgccgagc gtattgctaa gcttggtggt ttgcataaat ttatgaattg gggtaagcca atactaaccg attctggtgg ttttcaagta atgtctttat caaaattacg caagataacg gaagaggggg taagttttaa ctcgcatatt aatggtgata aatatttgct gacgcctgaa cgttctaccg aaatacaaca tctactcggc agcactatta ccatggcttt tgacgaatgt acgccttacc ctgcaacgtt tgaagaagca aaaacctcta tgcagctcac aactagatgg gcacatagat caagagaagc gtttgttaag cgagatggtt atgcacagtt tggtattatt caaggtagca cttacgaaga gttacgtgag caatcggcta aagatttaat agagctagat tttgaaggat atgctatagg cggtcttgca gttggggagg ggcaagaact tatgtttaaa gttcttgccc ctgatttcct accgcaaaat aaacctagat atttgatggg ggtaggaaag cctgctgata ttatcggagc agtcagcaga ggggtagata tgtttgactg cgtaatcccg actcggtcag gtcgtaatgg tcaggctttt acaaaatacg gcacggtaaa tatccgcaat agtaaatatg ctgaagataa cgatcctttg gaagctgatt gcctttgccc tgcttgtcaa aattatagca aagcctattt acatcacttg gtgagaatag gcgaaatact tggagcgatg ctaatgactt ggcacaatct tacatatttc caaaacctta tgagccgtat tagagagtat attgccttag gtaaagattt tgattttact acttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat