RibeA.00075.a

Delta-aminolevulinic acid dehydratase

CENTER ID: RibeA.00075.a
ORGANISM: Rickettsia bellii RML369-C
ASSOCIATED DISEASE: Typhus
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.00075.a.B1.GE35576 Full length( RibeA.00075.a ) 1 330
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|336407.7.peg.1046
RefSeq: YP_538090.1
UniProt: Q1RI13

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMRYIMYPLI RLRRNRKAAW LRDLVAETTL RVEDLVLPLF VVEGHNQRQE IKTLPDIYRL SIDQVIETAR KAADLGIKAI ALFPCIDQSL KNENGDEAYN LDNLMCRTIR SIKNAGINIG IICDVALDPY TTHGHDGIIH NGEVDNDKSV KALCNQALTL AKAGVDIVAP SDMMDGRIMA IREYLDKEHF INVGILAYAA KYASSFYGLY RDAVGSTKKT YLDKASYQLD IRNIKEAMLE IEHDIAEGAD MVMVKPGMMF LDVIREAANN FNTKIFAYQI TAEYAMLKFA ANAGAIDFEK GLLESLISFK RAGASGIFTY AALEAAELYL
NT Sequence
ATGATGAGAT ATATTATGTA CCCACTTATT AGACTTAGAA GAAATAGAAA AGCTGCTTGG CTTCGTGATT TAGTTGCTGA AACTACTCTT AGAGTAGAAG ATTTGGTATT ACCTTTATTT GTTGTGGAAG GGCATAATCA GCGACAAGAA ATTAAAACTT TGCCAGATAT ATATCGCCTA TCTATAGATC AGGTGATAGA AACGGCAAGA AAAGCAGCGG ATCTCGGTAT TAAAGCTATA GCACTATTTC CCTGTATTGA TCAAAGTTTA AAAAACGAGA ATGGTGACGA AGCTTATAAT CTAGATAATT TAATGTGCCG TACTATAAGA AGCATTAAAA ATGCTGGGAT CAATATTGGA ATAATATGTG ATGTTGCACT TGATCCTTAT ACTACGCACG GGCATGATGG CATAATACAT AACGGCGAAG TGGATAATGA TAAATCGGTA AAAGCCTTAT GTAACCAAGC TTTAACTCTT GCAAAAGCTG GTGTTGATAT TGTTGCTCCT TCTGATATGA TGGATGGTAG GATTATGGCT ATAAGAGAAT ATCTTGACAA AGAGCATTTT ATCAATGTTG GGATTCTTGC CTATGCTGCT AAATATGCTT CAAGTTTTTA CGGTTTATAT CGTGATGCAG TCGGTAGTAC TAAGAAAACT TATTTAGATA AGGCAAGTTA TCAATTGGAC ATACGAAATA TTAAAGAAGC AATGCTTGAG ATTGAACATG ATATAGCAGA GGGAGCTGAT ATGGTCATGG TTAAGCCCGG TATGATGTTT TTAGATGTTA TTCGTGAAGC AGCTAATAAC TTTAATACTA AAATTTTTGC TTATCAAATT ACTGCGGAAT ATGCCATGTT AAAATTTGCA GCAAATGCCG GAGCTATAGA TTTCGAAAAA GGTTTATTAG AATCATTAAT TAGTTTTAAA AGAGCAGGGG CAAGCGGTAT ATTTACTTAT GCAGCTCTTG AAGCAGCTGA ACTATACCTT
Details for RibeA.00075.a.B1.GE35576
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MMRYIMYPLI RLRRNRKAAW LRDLVAETTL RVEDLVLPLF VVEGHNQRQE IKTLPDIYRL SIDQVIETAR KAADLGIKAI ALFPCIDQSL KNENGDEAYN LDNLMCRTIR SIKNAGINIG IICDVALDPY TTHGHDGIIH NGEVDNDKSV KALCNQALTL AKAGVDIVAP SDMMDGRIMA IREYLDKEHF INVGILAYAA KYASSFYGLY RDAVGSTKKT YLDKASYQLD IRNIKEAMLE IEHDIAEGAD MVMVKPGMMF LDVIREAANN FNTKIFAYQI TAEYAMLKFA ANAGAIDFEK GLLESLISFK RAGASGIFTY A
Validated NT Sequence
gctgcataag taaatatacc gcttgcccct gctcttttaa aactaattaa tgattctaat aaaccttttt cgaaatctat agctccggca tttgctgcaa attttaacat ggcatattcc gcagtaattt gataagcaaa aattttagta ttaaagttat tagctgcttc acgaataaca tctaaaaaca tcataccggg cttaaccatg accatatcag ctccctctgc tatatcatgt tcaatctcaa gcattgcttc tttaatattt cgtatgtcca attgataact tgccttatct aaataagttt tcttagtact accgactgca tcacgatata aaccgtaaaa acttgaagca tatttagcag cataggcaag aatcccaaca ttgataaaat gctctttgtc aagatattct cttatagcca taatcctacc atccatcata tcagaaggag caacaatatc aacaccagct tttgcaagag ttaaagcttg gttacataag gcttttaccg atttatcatt atccacttcg ccgttatgta ttatgccatc atgcccgtgc gtagtataag gatcaagtgc aacatcacat attattccaa tattgatccc agcattttta atgcttctta tagtacggca cattaaatta tctagattat aagcttcgtc accattctcg ttttttaaac tttgatcaat acagggaaat agtgctatag ctttaatacc gagatccgct gcttttcttg ccgtttctat cacctgatct atagataggc gatatatatc tggcaaagtt ttaatttctt gtcgctgatt atgcccttcc acaacaaata aaggtaatac caaatcttct actctaagag tagtttcagc aactaaatca cgaagccaag cagcttttct atttcttcta agtctaataa gtgggtacat aatatatctc atcatatggt ggtggtgg
Expected Protein Sequence
MAHHHHHHMM RYIMYPLIRL RRNRKAAWLR DLVAETTLRV EDLVLPLFVV EGHNQRQEIK TLPDIYRLSI DQVIETARKA ADLGIKAIAL FPCIDQSLKN ENGDEAYNLD NLMCRTIRSI KNAGINIGII CDVALDPYTT HGHDGIIHNG EVDNDKSVKA LCNQALTLAK AGVDIVAPSD MMDGRIMAIR EYLDKEHFIN VGILAYAAKY ASSFYGLYRD AVGSTKKTYL DKASYQLDIR NIKEAMLEIE HDIAEGADMV MVKPGMMFLD VIREAANNFN TKIFAYQITA EYAMLKFAAN AGAIDFEKGL LESLISFKRA GASGIFTYAA LEAAELYL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatga gatatattat gtacccactt attagactta gaagaaatag aaaagctgct tggcttcgtg atttagttgc tgaaactact cttagagtag aagatttggt attaccttta tttgttgtgg aagggcataa tcagcgacaa gaaattaaaa ctttgccaga tatatatcgc ctatctatag atcaggtgat agaaacggca agaaaagcag cggatctcgg tattaaagct atagcactat ttccctgtat tgatcaaagt ttaaaaaacg agaatggtga cgaagcttat aatctagata atttaatgtg ccgtactata agaagcatta aaaatgctgg gatcaatatt ggaataatat gtgatgttgc acttgatcct tatactacgc acgggcatga tggcataata cataacggcg aagtggataa tgataaatcg gtaaaagcct tatgtaacca agctttaact cttgcaaaag ctggtgttga tattgttgct ccttctgata tgatggatgg taggattatg gctataagag aatatcttga caaagagcat tttatcaatg ttgggattct tgcctatgct gctaaatatg cttcaagttt ttacggttta tatcgtgatg cagtcggtag tactaagaaa acttatttag ataaggcaag ttatcaattg gacatacgaa atattaaaga agcaatgctt gagattgaac atgatatagc agagggagct gatatggtca tggttaagcc cggtatgatg tttttagatg ttattcgtga agcagctaat aactttaata ctaaaatttt tgcttatcaa attactgcgg aatatgccat gttaaaattt gcagcaaatg ccggagctat agatttcgaa aaaggtttat tagaatcatt aattagtttt aaaagagcag gggcaagcgg tatatttact tatgcagctc ttgaagcagc tgaactatac ctttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat