RibeA.00069.a

Protein RecA

CENTER ID: RibeA.00069.a
ORGANISM: Rickettsia bellii RML369-C
ASSOCIATED DISEASE: Typhus
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.00069.a.B1.GE35549 Full length( RibeA.00069.a ) 1 343
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|336407.7.peg.134
RefSeq: YP_537287.1
UniProt: Q1RKB6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNIDKEKAV AAALAQIEKS YGKGSVMKLG QRPNVDIESV STGSLGLDIA LGIGGIPKGR IIEIFGPESS GKTTLTLHLI AEAQKKGGTC AFIDAEHALD PAYAKKLGVN IDELIISQPD TGEQALEIAD TLIRSGGIDM IIIDSVAALV PKSEIEGEMG DAQMASQARL MSQALRKLTA SINRTNCITV FINQIRMKIG VMFGSPETTT GGNALKFYAS VRIDIRRIGS IKDKEEVIGS QTKVKVVKNK VSPPFKTADF DIMYGSGISK EGEIIDLGVK LDIIEKSGSW FSYNSVRIGQ GRENVKQYLK DNPKISNEIE KLVREKSSKV TNINFEQEEE VND
NT Sequence
ATGTCAAATA TAGATAAAGA AAAAGCAGTT GCTGCGGCTC TCGCACAGAT TGAAAAAAGC TATGGTAAAG GCTCGGTTAT GAAACTTGGT CAGCGTCCTA ATGTTGATAT AGAATCTGTA TCAACCGGTT CGCTTGGGCT TGATATAGCT CTTGGAATTG GCGGAATTCC TAAAGGTAGA ATAATTGAAA TTTTTGGTCC TGAAAGCTCG GGTAAAACTA CTCTCACATT GCATTTAATT GCTGAGGCAC AGAAAAAAGG CGGAACATGT GCATTTATTG ATGCTGAACA TGCGTTAGAT CCGGCTTATG CTAAAAAGCT TGGAGTCAAT ATTGACGAGC TTATCATATC TCAGCCTGAT ACAGGAGAGC AAGCTTTAGA AATTGCTGAT ACTTTAATTC GCTCCGGCGG TATTGATATG ATAATAATAG ATAGTGTTGC CGCTCTAGTA CCGAAATCAG AGATTGAAGG CGAGATGGGC GATGCACAAA TGGCTTCTCA AGCAAGATTA ATGAGTCAGG CTTTACGTAA ACTTACTGCC TCAATTAATC GTACTAACTG TATTACTGTT TTCATCAACC AAATTAGAAT GAAAATAGGT GTAATGTTTG GTAGTCCTGA AACTACAACC GGCGGTAATG CTTTAAAATT CTATGCTTCA GTTAGAATAG ATATCAGAAG AATAGGATCT ATTAAGGATA AAGAAGAAGT AATAGGCAGC CAAACCAAAG TAAAAGTAGT AAAAAACAAA GTATCACCTC CATTTAAAAC AGCAGATTTT GACATAATGT ATGGCTCAGG TATCTCAAAA GAAGGTGAAA TAATCGACCT TGGAGTGAAA CTTGACATAA TAGAAAAATC CGGCTCTTGG TTTTCTTATA ATAGCGTACG TATCGGTCAA GGGCGTGAAA ATGTTAAACA ATATTTAAAA GATAACCCAA AAATTTCTAA TGAAATTGAG AAGCTTGTAA GAGAAAAATC ATCTAAAGTC ACTAATATAA ATTTTGAACA AGAGGAGGAA GTAAATGAT
Details for RibeA.00069.a.B1.GE35549
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 38kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MSNIDKEKAV AAALAQIEKS YGKGSVMKLG QRPNVDIESV STGSLGLDIA LGIGGIPKGR IIEIFGPESS GKTTLTLHLI AEAQKKGGTC AFIDAEHALD PAYAKKLGVN IDELIISQPD TGEQALEIAD TLIRSGGIDM IIIDSVAALV PKSEIEGEMG DAQMASQARL MSQALRKLTA SINRTNCITV FINQIRMKIG VMFGSPETTT GGNALKFYAS VRIDIRRIGS IKDKEEVIGS QTKVKVVKNK VSPPFKTADF DIMYGSGISK EGEIIDLGVK LDIIEKSGSW FSYNSVRIGQ GRENVKQYLK DNPKISNEIE KLVREKSSKV TNIN
Validated NT Sequence
aaaatttata ttagtgactt tagatgattt ttctcttaca agcttctcaa tttcattaga aatttttggg ttatctttta aatattgttt aacattttca cgcccttgac cgatacgtac gctattataa gaaaaccaag agccggattt ttctattatg tcaagtttca ctccaaggtc gattatttca ccttcttttg agatacctga gccatacatt atgtcaaaat ctgctgtttt aaatggaggt gatactttgt tttttactac ttttactttg gtttggctgc ctattacttc ttctttatcc ttaatagatc ctattcttct gatatctatt ctaactgaag catagaattt taaagcatta ccgccggttg tagtttcagg actaccaaac attacaccta ttttcattct aatttggttg atgaaaacag taatacagtt agtacgatta attgaggcag taagtttacg taaagcctga ctcattaatc ttgcttgaga agccatttgt gcatcgccca tctcgccttc aatctctgat ttcggtacta gagcggcaac actatctatt attatcatat caataccgcc ggagcgaatt aaagtatcag caatttctaa agcttgctct cctgtatcag gctgagatat gataagctcg tcaatattga ctccaagctt tttagcataa gccggatcta acgcatgttc agcatcaata aatgcacatg ttccgccttt tttctgtgcc tcagcaatta aatgcaatgt gagagtagtt ttacccgagc tttcaggacc aaaaatttca attattctac ctttaggaat tccgccaatt ccaagagcta tatcaagccc aagcgaaccg gttgatacag attctatatc aacattagga cgctgaccaa gtttcataac cgagccttta ccatagcttt tttcaatctg tgcgagagcc gcagcaactg ctttttcttt atctatattt gacatatggt ggtggtggtg
Expected Protein Sequence
MAHHHHHHMS NIDKEKAVAA ALAQIEKSYG KGSVMKLGQR PNVDIESVST GSLGLDIALG IGGIPKGRII EIFGPESSGK TTLTLHLIAE AQKKGGTCAF IDAEHALDPA YAKKLGVNID ELIISQPDTG EQALEIADTL IRSGGIDMII IDSVAALVPK SEIEGEMGDA QMASQARLMS QALRKLTASI NRTNCITVFI NQIRMKIGVM FGSPETTTGG NALKFYASVR IDIRRIGSIK DKEEVIGSQT KVKVVKNKVS PPFKTADFDI MYGSGISKEG EIIDLGVKLD IIEKSGSWFS YNSVRIGQGR ENVKQYLKDN PKISNEIEKL VREKSSKVTN INFEQEEEVN D
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcaa atatagataa agaaaaagca gttgctgcgg ctctcgcaca gattgaaaaa agctatggta aaggctcggt tatgaaactt ggtcagcgtc ctaatgttga tatagaatct gtatcaaccg gttcgcttgg gcttgatata gctcttggaa ttggcggaat tcctaaaggt agaataattg aaatttttgg tcctgaaagc tcgggtaaaa ctactctcac attgcattta attgctgagg cacagaaaaa aggcggaaca tgtgcattta ttgatgctga acatgcgtta gatccggctt atgctaaaaa gcttggagtc aatattgacg agcttatcat atctcagcct gatacaggag agcaagcttt agaaattgct gatactttaa ttcgctccgg cggtattgat atgataataa tagatagtgt tgccgctcta gtaccgaaat cagagattga aggcgagatg ggcgatgcac aaatggcttc tcaagcaaga ttaatgagtc aggctttacg taaacttact gcctcaatta atcgtactaa ctgtattact gttttcatca accaaattag aatgaaaata ggtgtaatgt ttggtagtcc tgaaactaca accggcggta atgctttaaa attctatgct tcagttagaa tagatatcag aagaatagga tctattaagg ataaagaaga agtaataggc agccaaacca aagtaaaagt agtaaaaaac aaagtatcac ctccatttaa aacagcagat tttgacataa tgtatggctc aggtatctca aaagaaggtg aaataatcga ccttggagtg aaacttgaca taatagaaaa atccggctct tggttttctt ataatagcgt acgtatcggt caagggcgtg aaaatgttaa acaatattta aaagataacc caaaaatttc taatgaaatt gagaagcttg taagagaaaa atcatctaaa gtcactaata taaattttga acaagaggag gaagtaaatg attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat