RibeA.00061.a
UDP-N-acetylglucosamine 2-epimerase
| CENTER ID: | RibeA.00061.a |
| ORGANISM: | Rickettsia bellii RML369-C |
| ASSOCIATED DISEASE: | Typhus |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB/C |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RibeA.00061.a.B1.GE35579 | Full length( RibeA.00061.a ) | 1 | 377 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RibeA.00061.a.B1.PW36203 | Full length( RibeA.00061.a ) | 1 | 377 |
Structures
4HWG
DEPOSITED: 11/7/2012
DETERMINATION: XRay
CLONE: RibeA.00061.a.B1.GE35579
PROTEIN: RibeA.00061.a.B1.PW36203
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131791 |
| PATRIC ID: | fig|336407.7.peg.812 |
| RefSeq: | YP_537878.1 |
| UniProt: | Q1RIM5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKVMTIVGT RPELIKLCCV ISEFDKHTKH ILVHTGQNYA YELNQVFFDD MGIRKPDYFL EVAADNTAKS IGLVIEKVDE VLEKEKPDAV LFYGDTNSCL SAIAAKRRKI PIFHMEAGNR CFDQRVPEEI NRKIIDHISD VNITLTEHAR RYLIAEGLPA ELTFKSGSHM PEVLDRFMPK ILKSDILDKL SLTPKQYFLI SSHREENVDV KNNLKELLNS LQMLIKEYNF LIIFSTHPRT KKRLEDLEGF KELGDKIRFL PAFSFTDYVK LQMNAFCILS DSGTITEEAS ILNLPALNIR EAHERPEGMD AGTLIMSGFK AERVLQAVKT ITEEHDNNKR TQGLVPDYNE AGLVSKKILR IVLSYVDYIN RTVWFKW
NT Sequence
ATGCTTAAGG TAATGACTAT TGTCGGTACT CGCCCAGAGC TTATTAAGCT GTGTTGTGTG ATTTCTGAGT TTGATAAACA TACCAAACAT ATTCTAGTTC ATACCGGTCA AAATTATGCG TACGAATTAA ATCAGGTTTT TTTTGATGAT ATGGGAATCA GAAAACCAGA TTATTTTTTA GAGGTTGCAG CAGATAATAC AGCAAAATCT ATCGGACTTG TTATTGAAAA AGTAGATGAA GTTCTTGAAA AAGAAAAACC TGATGCTGTG CTATTTTACG GTGATACTAA TTCTTGTCTT TCTGCAATTG CTGCTAAACG TCGTAAGATT CCAATTTTTC ATATGGAAGC AGGAAATCGC TGCTTTGACC AAAGAGTACC AGAAGAAATA AACCGCAAAA TCATTGATCA CATTAGCGAT GTTAATATTA CATTAACCGA GCATGCAAGA CGTTATTTAA TTGCTGAAGG ACTACCGGCG GAGCTTACCT TTAAATCAGG TTCACATATG CCAGAAGTAC TTGATCGCTT CATGCCTAAA ATATTAAAGT CAGATATTCT AGATAAATTA TCACTCACAC CAAAACAATA TTTCTTAATT AGCTCACATC GTGAAGAAAA TGTAGACGTC AAAAATAATC TAAAAGAATT ATTAAATAGT TTACAAATGC TTATTAAAGA GTATAATTTT TTGATAATCT TTTCTACGCA TCCGAGAACT AAAAAAAGGC TTGAAGATTT AGAAGGGTTT AAAGAGCTTG GAGATAAAAT AAGATTTTTA CCGGCTTTTA GTTTTACCGA TTATGTAAAA TTACAAATGA ATGCTTTTTG TATTTTATCG GATAGCGGTA CTATCACTGA AGAAGCATCA ATACTTAATT TGCCTGCCTT AAATATTAGA GAGGCTCACG AGCGTCCTGA AGGTATGGAT GCCGGCACGT TAATTATGTC CGGTTTTAAG GCTGAACGGG TATTACAAGC GGTAAAGACT ATTACCGAGG AGCATGATAA CAACAAACGT ACTCAAGGCT TAGTTCCTGA TTATAACGAA GCAGGGCTTG TATCGAAAAA AATATTGCGT ATAGTTTTAA GTTATGTGGA TTATATTAAT CGTACAGTCT GGTTTAAATG G
Details for RibeA.00061.a.B1.GE35579
| HARVESTED ON: | 6/12/2012 |
| SEQUENCED ON: | 6/12/2012 |
| EXPECTED MW: | 44kDa |
| OBSERVED MW: | 44kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 67 |
Validated AA Sequence
MAHHHHHHML KVMTIVGTRP ELIKLCCVIS EFDKHTKHIL VHTGQNYAYE LNQVFFDDMG IRKPDYFLEV AADNTAKSIG LVIEKVDEVL EKEKPDAVLF YGDTNSCLSA IAAKRRKIPI FHMEAGNRCF DQRVPEEINR KIIDHISDVN ITLTEHARRY LIAEGLPAEL TFKSGSHMPE VLDRFMPKIL KSDILDKLSL TPKQYFLISS HREENVDVKN NLKELLNSLQ MLIKEYNFLI IFSTHPRTKK RLEXLEXV
Validated NT Sequence
ttgnnnnnct ttaagaagga gatataccat ggctcaccac caccaccacc atatgcttaa ggtaatgact attgtcggta ctcgcccaga gcttattaag ctgtgttgtg tgatttctga gtttgataaa cataccaaac atattctagt tcataccggt caaaattatg cgtacgaatt aaatcaggtt ttttttgatg atatgggaat cagaaaacca gattattttt tagaggttgc agcagataat acagcaaaat ctatcggact tgttattgaa aaagtagatg aagttcttga aaaagaaaaa cctgatgctg tgctatttta cggtgatact aattcttgtc tttctgcaat tgctgctaaa cgtcgtaaga ttccaatttt tcatatggaa gcaggaaatc gctgctttga ccaaagagta ccagaagaaa taaaccgcaa aatcattgat cacattagcg atgttaatat tacattaacc gagcatgcaa gacgttattt aattgctgaa ggactaccgg cggagcttac ctttaaatca ggttcacata tgccagaagt acttgatcgc ttcatgccta aaatattaaa gtcagatatt ctagataaat tatcactcac accaaaacaa tatttcttaa ttagctcaca tcgtgaagaa aatgtagacg tcaaaaataa tctaaaagaa ttattaaata gtttacaaat gcttattaaa gagtataatt ttttgataat cttttctacg catccgagaa ctaaaaaaag gcttgaagan ttagaanggg tttaaagagc ttggngataa ataagatttt taccggcttt tanttttacc gantatgtaa aattacaatg aatgcttttt gnattttatc ggatagcggt actatcactg aagaancatc antacttaat ttgnctgnct taannnntag anaggnncac gagcgtcctg naggnatgga tgncnggcac nnnnnnntgt cngnnttnnn nngannggta ttnngcngna anannnntnn cnagnannat nntaacnncn angtactnan tnnnnnnnnn nnnannnnnn nnnntnnnan aaaatnnnnn nnnnnnnnnn ancnnnnnnn nnnnnnnnnn nnnnnnnnnn ancgnnnnnn nnnaancc
Expected Protein Sequence
MAHHHHHHML KVMTIVGTRP ELIKLCCVIS EFDKHTKHIL VHTGQNYAYE LNQVFFDDMG IRKPDYFLEV AADNTAKSIG LVIEKVDEVL EKEKPDAVLF YGDTNSCLSA IAAKRRKIPI FHMEAGNRCF DQRVPEEINR KIIDHISDVN ITLTEHARRY LIAEGLPAEL TFKSGSHMPE VLDRFMPKIL KSDILDKLSL TPKQYFLISS HREENVDVKN NLKELLNSLQ MLIKEYNFLI IFSTHPRTKK RLEDLEGFKE LGDKIRFLPA FSFTDYVKLQ MNAFCILSDS GTITEEASIL NLPALNIREA HERPEGMDAG TLIMSGFKAE RVLQAVKTIT EEHDNNKRTQ GLVPDYNEAG LVSKKILRIV LSYVDYINRT VWFKW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctta aggtaatgac tattgtcggt actcgcccag agcttattaa gctgtgttgt gtgatttctg agtttgataa acataccaaa catattctag ttcataccgg tcaaaattat gcgtacgaat taaatcaggt tttttttgat gatatgggaa tcagaaaacc agattatttt ttagaggttg cagcagataa tacagcaaaa tctatcggac ttgttattga aaaagtagat gaagttcttg aaaaagaaaa acctgatgct gtgctatttt acggtgatac taattcttgt ctttctgcaa ttgctgctaa acgtcgtaag attccaattt ttcatatgga agcaggaaat cgctgctttg accaaagagt accagaagaa ataaaccgca aaatcattga tcacattagc gatgttaata ttacattaac cgagcatgca agacgttatt taattgctga aggactaccg gcggagctta cctttaaatc aggttcacat atgccagaag tacttgatcg cttcatgcct aaaatattaa agtcagatat tctagataaa ttatcactca caccaaaaca atatttctta attagctcac atcgtgaaga aaatgtagac gtcaaaaata atctaaaaga attattaaat agtttacaaa tgcttattaa agagtataat tttttgataa tcttttctac gcatccgaga actaaaaaaa ggcttgaaga tttagaaggg tttaaagagc ttggagataa aataagattt ttaccggctt ttagttttac cgattatgta aaattacaaa tgaatgcttt ttgtatttta tcggatagcg gtactatcac tgaagaagca tcaatactta atttgcctgc cttaaatatt agagaggctc acgagcgtcc tgaaggtatg gatgccggca cgttaattat gtccggtttt aaggctgaac gggtattaca agcggtaaag actattaccg aggagcatga taacaacaaa cgtactcaag gcttagttcc tgattataac gaagcagggc ttgtatcgaa aaaaatattg cgtatagttt taagttatgt ggattatatt aatcgtacag tctggtttaa atggtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for RibeA.00061.a.B1.PW36203
| PURIFICATION DATe: | 8/13/2012 |
| CONCENTRATION: | 29.73mg/ml |
| OBSERVED MW: | 43kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 15 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 67 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML KVMTIVGTRP ELIKLCCVIS EFDKHTKHIL VHTGQNYAYE LNQVFFDDMG IRKPDYFLEV AADNTAKSIG LVIEKVDEVL EKEKPDAVLF YGDTNSCLSA IAAKRRKIPI FHMEAGNRCF DQRVPEEINR KIIDHISDVN ITLTEHARRY LIAEGLPAEL TFKSGSHMPE VLDRFMPKIL KSDILDKLSL TPKQYFLISS HREENVDVKN NLKELLNSLQ MLIKEYNFLI IFSTHPRTKK RLEXLEXV
Validated NT Sequence
ttgnnnnnct ttaagaagga gatataccat ggctcaccac caccaccacc atatgcttaa ggtaatgact attgtcggta ctcgcccaga gcttattaag ctgtgttgtg tgatttctga gtttgataaa cataccaaac atattctagt tcataccggt caaaattatg cgtacgaatt aaatcaggtt ttttttgatg atatgggaat cagaaaacca gattattttt tagaggttgc agcagataat acagcaaaat ctatcggact tgttattgaa aaagtagatg aagttcttga aaaagaaaaa cctgatgctg tgctatttta cggtgatact aattcttgtc tttctgcaat tgctgctaaa cgtcgtaaga ttccaatttt tcatatggaa gcaggaaatc gctgctttga ccaaagagta ccagaagaaa taaaccgcaa aatcattgat cacattagcg atgttaatat tacattaacc gagcatgcaa gacgttattt aattgctgaa ggactaccgg cggagcttac ctttaaatca ggttcacata tgccagaagt acttgatcgc ttcatgccta aaatattaaa gtcagatatt ctagataaat tatcactcac accaaaacaa tatttcttaa ttagctcaca tcgtgaagaa aatgtagacg tcaaaaataa tctaaaagaa ttattaaata gtttacaaat gcttattaaa gagtataatt ttttgataat cttttctacg catccgagaa ctaaaaaaag gcttgaagan ttagaanggg tttaaagagc ttggngataa ataagatttt taccggcttt tanttttacc gantatgtaa aattacaatg aatgcttttt gnattttatc ggatagcggt actatcactg aagaancatc antacttaat ttgnctgnct taannnntag anaggnncac gagcgtcctg naggnatgga tgncnggcac nnnnnnntgt cngnnttnnn nngannggta ttnngcngna anannnntnn cnagnannat nntaacnncn angtactnan tnnnnnnnnn nnnannnnnn nnnntnnnan aaaatnnnnn nnnnnnnnnn ancnnnnnnn nnnnnnnnnn nnnnnnnnnn ancgnnnnnn nnnaancc
Expressed Protein Sequence
MAHHHHHHML KVMTIVGTRP ELIKLCCVIS EFDKHTKHIL VHTGQNYAYE LNQVFFDDMG IRKPDYFLEV AADNTAKSIG LVIEKVDEVL EKEKPDAVLF YGDTNSCLSA IAAKRRKIPI FHMEAGNRCF DQRVPEEINR KIIDHISDVN ITLTEHARRY LIAEGLPAEL TFKSGSHMPE VLDRFMPKIL KSDILDKLSL TPKQYFLISS HREENVDVKN NLKELLNSLQ MLIKEYNFLI IFSTHPRTKK RLEDLEGFKE LGDKIRFLPA FSFTDYVKLQ MNAFCILSDS GTITEEASIL NLPALNIREA HERPEGMDAG TLIMSGFKAE RVLQAVKTIT EEHDNNKRTQ GLVPDYNEAG LVSKKILRIV LSYVDYINRT VWFKW
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTTA AGGTAATGAC TATTGTCGGT ACTCGCCCAG AGCTTATTAA GCTGTGTTGT GTGATTTCTG AGTTTGATAA ACATACCAAA CATATTCTAG TTCATACCGG TCAAAATTAT GCGTACGAAT TAAATCAGGT TTTTTTTGAT GATATGGGAA TCAGAAAACC AGATTATTTT TTAGAGGTTG CAGCAGATAA TACAGCAAAA TCTATCGGAC TTGTTATTGA AAAAGTAGAT GAAGTTCTTG AAAAAGAAAA ACCTGATGCT GTGCTATTTT ACGGTGATAC TAATTCTTGT CTTTCTGCAA TTGCTGCTAA ACGTCGTAAG ATTCCAATTT TTCATATGGA AGCAGGAAAT CGCTGCTTTG ACCAAAGAGT ACCAGAAGAA ATAAACCGCA AAATCATTGA TCACATTAGC GATGTTAATA TTACATTAAC CGAGCATGCA AGACGTTATT TAATTGCTGA AGGACTACCG GCGGAGCTTA CCTTTAAATC AGGTTCACAT ATGCCAGAAG TACTTGATCG CTTCATGCCT AAAATATTAA AGTCAGATAT TCTAGATAAA TTATCACTCA CACCAAAACA ATATTTCTTA ATTAGCTCAC ATCGTGAAGA AAATGTAGAC GTCAAAAATA ATCTAAAAGA ATTATTAAAT AGTTTACAAA TGCTTATTAA AGAGTATAAT TTTTTGATAA TCTTTTCTAC GCATCCGAGA ACTAAAAAAA GGCTTGAAGA TTTAGAAGGG TTTAAAGAGC TTGGAGATAA AATAAGATTT TTACCGGCTT TTAGTTTTAC CGATTATGTA AAATTACAAA TGAATGCTTT TTGTATTTTA TCGGATAGCG GTACTATCAC TGAAGAAGCA TCAATACTTA ATTTGCCTGC CTTAAATATT AGAGAGGCTC ACGAGCGTCC TGAAGGTATG GATGCCGGCA CGTTAATTAT GTCCGGTTTT AAGGCTGAAC GGGTATTACA AGCGGTAAAG ACTATTACCG AGGAGCATGA TAACAACAAA CGTACTCAAG GCTTAGTTCC TGATTATAAC GAAGCAGGGC TTGTATCGAA AAAAATATTG CGTATAGTTT TAAGTTATGT GGATTATATT AATCGTACAG TCTGGTTTAA ATGGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT