RibeA.00057.d

ABC-type multidrug transport system, ATPase component

CENTER ID: RibeA.00057.d
ORGANISM: Rickettsia bellii RML369-C
ASSOCIATED DISEASE: Typhus
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.00057.d.B1.GE35578 Full length( RibeA.00057.d ) 1 256
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.00057.d.B1.PW36202 Full length( RibeA.00057.d ) 1 256

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|336407.7.peg.318
RefSeq: YP_537452.1
UniProt: Q1RJV1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIIKKGEIIF NKVKFQYKNT TPLFQNKSLV IKPGGKVGLV GYSGSGKTTF VNLILRMYDI AEGKIVIDDQ DIKEITQESL RNNIGVIPQE ASLFHRSLME NIRYGRPDAS DAEVIEAAKK AYAHEFIEKL PLGYESLVGE RGIKLSGGQR QRIAIARAIL KNAPILILDE ATSQLDSVTE EYIQKSLWDL MQGKTTIVIA HRLSTLLHMD RIIVFDKGRI VEDGSHNELL DRNGLYKTLW DSQIGGFLPD KELAVV
NT Sequence
TTGATAATTA AAAAGGGAGA GATAATTTTT AATAAAGTAA AATTCCAATA TAAAAATACT ACGCCGTTAT TTCAAAATAA ATCGTTAGTA ATCAAGCCCG GGGGAAAAGT AGGGTTGGTA GGTTATTCCG GAAGTGGCAA GACAACTTTT GTTAATTTGA TCCTTAGAAT GTATGATATA GCAGAAGGAA AAATAGTTAT TGACGATCAA GATATTAAAG AAATTACGCA AGAGTCGCTA CGTAATAATA TAGGCGTAAT ACCGCAAGAA GCTTCACTTT TTCATCGTAG CCTTATGGAA AATATTAGAT ATGGTAGACC TGATGCTTCA GATGCAGAAG TAATAGAGGC TGCTAAAAAA GCTTATGCAC ATGAGTTTAT TGAAAAATTA CCGCTTGGTT ATGAGTCGTT AGTTGGTGAG CGAGGTATCA AGCTATCAGG CGGACAAAGA CAGAGAATAG CAATCGCAAG GGCTATTCTT AAAAATGCTC CAATTTTGAT TTTAGACGAA GCCACTTCAC AACTAGATTC TGTGACTGAG GAATATATTC AAAAAAGCTT ATGGGATTTA ATGCAAGGAA AAACAACGAT AGTAATTGCC CACCGTTTAT CAACGCTCTT GCATATGGAT AGGATTATTG TTTTTGATAA AGGTAGAATT GTCGAAGACG GCAGCCATAA CGAGCTACTA GATCGTAACG GGTTATATAA GACTTTATGG GACTCTCAAA TTGGTGGCTT TTTGCCTGAT AAAGAATTAG CTGTTGTG
Details for RibeA.00057.d.B1.GE35578
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML IIKKGEIIFN KVKFQYKNTT PLFQNKSLVI KPGGKVGLVG YSGSGKTTFV NLILRMYDIA EGKIVIDDQD IKEITQESLR NNIGVIPQEA SLFHRSLMEN IRYGRPDASD AEVIEAAKKA YAHEFIEKLP LGYESLVGER GIKLSGGQRQ RIAIARAILK NAPILILDEA TSQLDSVTEE YIQKSLWDLM QGKTTIVIAH RLSTLLHMDR IIVFDKGRIV EDGSHNELLD RNGLYKTLWD SQIGGFLPDK ELAVCK
Validated NT Sequence
atcttactca cttacacaca gctaattctt tatcaggcaa aaagccacca atttgagagt cccataaagt cttatataac ccgttacgat ctagtagctc gttatggctg ccgtcttcga caattctacc tttatcaaaa acaataatcc tatccatatg caagagcgtt gataaacggt gggcaattac tatcgttgtt tttccttgca ttaaatccca taagcttttt tgaatatatt cctcagtcac agaatctagt tgtgaagtgg cttcgtctaa aatcaaaatt ggagcatttt taagaatagc ccttgcgatt gctattctct gtctttgtcc gcctgatagc ttgatacctc gctcaccaac taacgactca taaccaagcg gtaatttttc aataaactca tgtgcataag cttttttagc agcctctatt acttctgcat ctgaagcatc aggtctacca tatctaatat tttccataag gctacgatga aaaagtgaag cttcttgcgg tattacgcct atattattac gtagcgactc ttgcgtaatt tctttaatat cttgatcgtc aataactatt tttccttctg ctatatcata cattctaagg atcaaattaa caaaagttgt cttgccactt ccggaataac ctaccaaccc tacttttccc ccgggcttga ttactaacga tttattttga aataacggcg tagtattttt atattggaat tttactttat taaaaattat ctctcccttt ttaattatca acatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHML IIKKGEIIFN KVKFQYKNTT PLFQNKSLVI KPGGKVGLVG YSGSGKTTFV NLILRMYDIA EGKIVIDDQD IKEITQESLR NNIGVIPQEA SLFHRSLMEN IRYGRPDASD AEVIEAAKKA YAHEFIEKLP LGYESLVGER GIKLSGGQRQ RIAIARAILK NAPILILDEA TSQLDSVTEE YIQKSLWDLM QGKTTIVIAH RLSTLLHMDR IIVFDKGRIV EDGSHNELLD RNGLYKTLWD SQIGGFLPDK ELAVV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga taattaaaaa gggagagata atttttaata aagtaaaatt ccaatataaa aatactacgc cgttatttca aaataaatcg ttagtaatca agcccggggg aaaagtaggg ttggtaggtt attccggaag tggcaagaca acttttgtta atttgatcct tagaatgtat gatatagcag aaggaaaaat agttattgac gatcaagata ttaaagaaat tacgcaagag tcgctacgta ataatatagg cgtaataccg caagaagctt cactttttca tcgtagcctt atggaaaata ttagatatgg tagacctgat gcttcagatg cagaagtaat agaggctgct aaaaaagctt atgcacatga gtttattgaa aaattaccgc ttggttatga gtcgttagtt ggtgagcgag gtatcaagct atcaggcgga caaagacaga gaatagcaat cgcaagggct attcttaaaa atgctccaat tttgatttta gacgaagcca cttcacaact agattctgtg actgaggaat atattcaaaa aagcttatgg gatttaatgc aaggaaaaac aacgatagta attgcccacc gtttatcaac gctcttgcat atggatagga ttattgtttt tgataaaggt agaattgtcg aagacggcag ccataacgag ctactagatc gtaacgggtt atataagact ttatgggact ctcaaattgg tggctttttg cctgataaag aattagctgt tgtgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for RibeA.00057.d.B1.PW36202
PURIFICATION DATe: 7/23/2012
CONCENTRATION: 22mg/ml
OBSERVED MW: 29kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 24
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML IIKKGEIIFN KVKFQYKNTT PLFQNKSLVI KPGGKVGLVG YSGSGKTTFV NLILRMYDIA EGKIVIDDQD IKEITQESLR NNIGVIPQEA SLFHRSLMEN IRYGRPDASD AEVIEAAKKA YAHEFIEKLP LGYESLVGER GIKLSGGQRQ RIAIARAILK NAPILILDEA TSQLDSVTEE YIQKSLWDLM QGKTTIVIAH RLSTLLHMDR IIVFDKGRIV EDGSHNELLD RNGLYKTLWD SQIGGFLPDK ELAVCK
Validated NT Sequence
atcttactca cttacacaca gctaattctt tatcaggcaa aaagccacca atttgagagt cccataaagt cttatataac ccgttacgat ctagtagctc gttatggctg ccgtcttcga caattctacc tttatcaaaa acaataatcc tatccatatg caagagcgtt gataaacggt gggcaattac tatcgttgtt tttccttgca ttaaatccca taagcttttt tgaatatatt cctcagtcac agaatctagt tgtgaagtgg cttcgtctaa aatcaaaatt ggagcatttt taagaatagc ccttgcgatt gctattctct gtctttgtcc gcctgatagc ttgatacctc gctcaccaac taacgactca taaccaagcg gtaatttttc aataaactca tgtgcataag cttttttagc agcctctatt acttctgcat ctgaagcatc aggtctacca tatctaatat tttccataag gctacgatga aaaagtgaag cttcttgcgg tattacgcct atattattac gtagcgactc ttgcgtaatt tctttaatat cttgatcgtc aataactatt tttccttctg ctatatcata cattctaagg atcaaattaa caaaagttgt cttgccactt ccggaataac ctaccaaccc tacttttccc ccgggcttga ttactaacga tttattttga aataacggcg tagtattttt atattggaat tttactttat taaaaattat ctctcccttt ttaattatca acatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHML IIKKGEIIFN KVKFQYKNTT PLFQNKSLVI KPGGKVGLVG YSGSGKTTFV NLILRMYDIA EGKIVIDDQD IKEITQESLR NNIGVIPQEA SLFHRSLMEN IRYGRPDASD AEVIEAAKKA YAHEFIEKLP LGYESLVGER GIKLSGGQRQ RIAIARAILK NAPILILDEA TSQLDSVTEE YIQKSLWDLM QGKTTIVIAH RLSTLLHMDR IIVFDKGRIV EDGSHNELLD RNGLYKTLWD SQIGGFLPDK ELAVV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTGA TAATTAAAAA GGGAGAGATA ATTTTTAATA AAGTAAAATT CCAATATAAA AATACTACGC CGTTATTTCA AAATAAATCG TTAGTAATCA AGCCCGGGGG AAAAGTAGGG TTGGTAGGTT ATTCCGGAAG TGGCAAGACA ACTTTTGTTA ATTTGATCCT TAGAATGTAT GATATAGCAG AAGGAAAAAT AGTTATTGAC GATCAAGATA TTAAAGAAAT TACGCAAGAG TCGCTACGTA ATAATATAGG CGTAATACCG CAAGAAGCTT CACTTTTTCA TCGTAGCCTT ATGGAAAATA TTAGATATGG TAGACCTGAT GCTTCAGATG CAGAAGTAAT AGAGGCTGCT AAAAAAGCTT ATGCACATGA GTTTATTGAA AAATTACCGC TTGGTTATGA GTCGTTAGTT GGTGAGCGAG GTATCAAGCT ATCAGGCGGA CAAAGACAGA GAATAGCAAT CGCAAGGGCT ATTCTTAAAA ATGCTCCAAT TTTGATTTTA GACGAAGCCA CTTCACAACT AGATTCTGTG ACTGAGGAAT ATATTCAAAA AAGCTTATGG GATTTAATGC AAGGAAAAAC AACGATAGTA ATTGCCCACC GTTTATCAAC GCTCTTGCAT ATGGATAGGA TTATTGTTTT TGATAAAGGT AGAATTGTCG AAGACGGCAG CCATAACGAG CTACTAGATC GTAACGGGTT ATATAAGACT TTATGGGACT CTCAAATTGG TGGCTTTTTG CCTGATAAAG AATTAGCTGT TGTGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT