RibeA.00057.b

ABC transporter ATP-binding protein

CENTER ID: RibeA.00057.b
ORGANISM: Rickettsia bellii RML369-C
ASSOCIATED DISEASE: Typhus
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.00057.b.B1.GE35571 Full length( RibeA.00057.b ) 1 242
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RibeA.00057.b.B1.PS01593 Full length( RibeA.00057.b ) 1 242

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|336407.7.peg.994
RefSeq: YP_538043.1
UniProt: Q1RI60

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKPYLYAEKV QFKVKERNEP IISETTLNIA EEEFVVVLGT NGSGKSTLTK ILAGYLKPTS GQVFLDQMRI DKLPQLQKAN MLLTITQKAE DRLFSELTLE ENITLWESRF PANERKTDVE VLELIGLSKS KHFLSRLTQP LSNFSGGEKQ LILLALAISH PPKILFLDEH TASLDPKASH EVMKKTAEII DEHKITAVMI THNLEDAVNY GKRLIVLDNG KVAQDYLKPP AFSMKELKKI LG
NT Sequence
ATGAAACCTT ATTTATATGC TGAGAAAGTG CAGTTTAAAG TAAAAGAGCG TAATGAGCCG ATAATATCGG AAACTACTTT AAATATTGCC GAAGAAGAGT TTGTTGTAGT GCTTGGGACT AATGGCAGCG GTAAATCTAC TTTAACCAAA ATTCTAGCAG GTTATTTAAA GCCGACTAGC GGGCAGGTGT TTTTAGATCA AATGCGAATT GATAAATTAC CGCAATTGCA AAAAGCTAAT ATGTTGCTAA CTATAACCCA AAAAGCAGAA GATAGATTGT TTTCGGAGTT AACATTAGAA GAAAATATTA CCCTATGGGA GAGCCGCTTT CCAGCAAATG AGCGTAAAAC AGATGTGGAA GTATTAGAGC TTATCGGTTT ATCGAAATCT AAGCATTTTT TGTCACGTCT AACGCAGCCA CTTAGTAATT TTTCCGGTGG GGAGAAACAG CTTATATTGC TGGCACTAGC TATTTCTCAT CCGCCTAAAA TATTATTTCT AGATGAACAT ACTGCAAGCC TTGACCCTAA AGCTTCGCAT GAAGTGATGA AAAAAACGGC AGAGATTATT GATGAGCATA AAATAACTGC CGTAATGATC ACACATAATT TGGAAGATGC TGTAAATTAC GGAAAAAGAC TTATAGTTTT AGACAATGGT AAGGTAGCAC AAGACTACCT AAAACCACCG GCTTTTTCTA TGAAAGAATT GAAAAAGATA TTAGGG
Details for RibeA.00057.b.B1.GE35571
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/14/2012
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK PYLYAEKVQF KVKERNEPII SETTLNIAEE EFVVVLGTNG SGKSTLTKIL AGYLKPTSGQ VFLDQMRIDK LPQLQKANML LTITQKAEDR LFSELTLEEN ITLWESRFPA NERKTDVEVL ELIGLSKSKH FLSRLTQPLS NFSGGEKQLI LLALAISHPP KILFLDEHTA SLDPKASHEV MKKTAEIIDE HKITAVMITH NLEDAVNYGK RLIVLDNGKV AQDYLKPPAF SMKELKKILG
Validated NT Sequence
tcttactcac ttaccctaat atctttttca attctttcat agaaaaagcc ggtggtttta ggtagtcttg tgctacctta ccattgtcta aaactataag tctttttccg taatttacag catcttccaa attatgtgtg atcattacgg cagttatttt atgctcatca ataatctctg ccgttttttt catcacttca tgcgaagctt tagggtcaag gcttgcagta tgttcatcta gaaataatat tttaggcgga tgagaaatag ctagtgccag caatataagc tgtttctccc caccggaaaa attactaagt ggctgcgtta gacgtgacaa aaaatgctta gatttcgata aaccgataag ctctaatact tccacatctg ttttacgctc atttgctgga aagcggctct cccatagggt aatattttct tctaatgtta actccgaaaa caatctatct tctgcttttt gggttatagt tagcaacata ttagcttttt gcaattgcgg taatttatca attcgcattt gatctaaaaa cacctgcccg ctagtcggct ttaaataacc tgctagaatt ttggttaaag tagatttacc gctgccatta gtcccaagca ctacaacaaa ctcttcttcg gcaatattta aagtagtttc cgatattatc ggctcattac gctcttttac tttaaactgc actttctcag catataaata aggtttcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMK PYLYAEKVQF KVKERNEPII SETTLNIAEE EFVVVLGTNG SGKSTLTKIL AGYLKPTSGQ VFLDQMRIDK LPQLQKANML LTITQKAEDR LFSELTLEEN ITLWESRFPA NERKTDVEVL ELIGLSKSKH FLSRLTQPLS NFSGGEKQLI LLALAISHPP KILFLDEHTA SLDPKASHEV MKKTAEIIDE HKITAVMITH NLEDAVNYGK RLIVLDNGKV AQDYLKPPAF SMKELKKILG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac cttatttata tgctgagaaa gtgcagttta aagtaaaaga gcgtaatgag ccgataatat cggaaactac tttaaatatt gccgaagaag agtttgttgt agtgcttggg actaatggca gcggtaaatc tactttaacc aaaattctag caggttattt aaagccgact agcgggcagg tgtttttaga tcaaatgcga attgataaat taccgcaatt gcaaaaagct aatatgttgc taactataac ccaaaaagca gaagatagat tgttttcgga gttaacatta gaagaaaata ttaccctatg ggagagccgc tttccagcaa atgagcgtaa aacagatgtg gaagtattag agcttatcgg tttatcgaaa tctaagcatt ttttgtcacg tctaacgcag ccacttagta atttttccgg tggggagaaa cagcttatat tgctggcact agctatttct catccgccta aaatattatt tctagatgaa catactgcaa gccttgaccc taaagcttcg catgaagtga tgaaaaaaac ggcagagatt attgatgagc ataaaataac tgccgtaatg atcacacata atttggaaga tgctgtaaat tacggaaaaa gacttatagt tttagacaat ggtaaggtag cacaagacta cctaaaacca ccggcttttt ctatgaaaga attgaaaaag atattagggt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for RibeA.00057.b.B1.PS01593
PURIFICATION DATe: 8/15/2012
CONCENTRATION: 10.9mg/ml
OBSERVED MW: 26kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK PYLYAEKVQF KVKERNEPII SETTLNIAEE EFVVVLGTNG SGKSTLTKIL AGYLKPTSGQ VFLDQMRIDK LPQLQKANML LTITQKAEDR LFSELTLEEN ITLWESRFPA NERKTDVEVL ELIGLSKSKH FLSRLTQPLS NFSGGEKQLI LLALAISHPP KILFLDEHTA SLDPKASHEV MKKTAEIIDE HKITAVMITH NLEDAVNYGK RLIVLDNGKV AQDYLKPPAF SMKELKKILG
Validated NT Sequence
tcttactcac ttaccctaat atctttttca attctttcat agaaaaagcc ggtggtttta ggtagtcttg tgctacctta ccattgtcta aaactataag tctttttccg taatttacag catcttccaa attatgtgtg atcattacgg cagttatttt atgctcatca ataatctctg ccgttttttt catcacttca tgcgaagctt tagggtcaag gcttgcagta tgttcatcta gaaataatat tttaggcgga tgagaaatag ctagtgccag caatataagc tgtttctccc caccggaaaa attactaagt ggctgcgtta gacgtgacaa aaaatgctta gatttcgata aaccgataag ctctaatact tccacatctg ttttacgctc atttgctgga aagcggctct cccatagggt aatattttct tctaatgtta actccgaaaa caatctatct tctgcttttt gggttatagt tagcaacata ttagcttttt gcaattgcgg taatttatca attcgcattt gatctaaaaa cacctgcccg ctagtcggct ttaaataacc tgctagaatt ttggttaaag tagatttacc gctgccatta gtcccaagca ctacaacaaa ctcttcttcg gcaatattta aagtagtttc cgatattatc ggctcattac gctcttttac tttaaactgc actttctcag catataaata aggtttcata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMK PYLYAEKVQF KVKERNEPII SETTLNIAEE EFVVVLGTNG SGKSTLTKIL AGYLKPTSGQ VFLDQMRIDK LPQLQKANML LTITQKAEDR LFSELTLEEN ITLWESRFPA NERKTDVEVL ELIGLSKSKH FLSRLTQPLS NFSGGEKQLI LLALAISHPP KILFLDEHTA SLDPKASHEV MKKTAEIIDE HKITAVMITH NLEDAVNYGK RLIVLDNGKV AQDYLKPPAF SMKELKKILG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAC CTTATTTATA TGCTGAGAAA GTGCAGTTTA AAGTAAAAGA GCGTAATGAG CCGATAATAT CGGAAACTAC TTTAAATATT GCCGAAGAAG AGTTTGTTGT AGTGCTTGGG ACTAATGGCA GCGGTAAATC TACTTTAACC AAAATTCTAG CAGGTTATTT AAAGCCGACT AGCGGGCAGG TGTTTTTAGA TCAAATGCGA ATTGATAAAT TACCGCAATT GCAAAAAGCT AATATGTTGC TAACTATAAC CCAAAAAGCA GAAGATAGAT TGTTTTCGGA GTTAACATTA GAAGAAAATA TTACCCTATG GGAGAGCCGC TTTCCAGCAA ATGAGCGTAA AACAGATGTG GAAGTATTAG AGCTTATCGG TTTATCGAAA TCTAAGCATT TTTTGTCACG TCTAACGCAG CCACTTAGTA ATTTTTCCGG TGGGGAGAAA CAGCTTATAT TGCTGGCACT AGCTATTTCT CATCCGCCTA AAATATTATT TCTAGATGAA CATACTGCAA GCCTTGACCC TAAAGCTTCG CATGAAGTGA TGAAAAAAAC GGCAGAGATT ATTGATGAGC ATAAAATAAC TGCCGTAATG ATCACACATA ATTTGGAAGA TGCTGTAAAT TACGGAAAAA GACTTATAGT TTTAGACAAT GGTAAGGTAG CACAAGACTA CCTAAAACCA CCGGCTTTTT CTATGAAAGA ATTGAAAAAG ATATTAGGGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT