SSGCID: Target Page

RiafA.18411.a

VirB10

CENTER ID:	RiafA.18411.a
ORGANISM:	Rickettsia africae ESF-5
ASSOCIATED DISEASE:	African tick-bite fever
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiafA.18411.a.B2.GE39984	soluble domain		58	482
RiafA.18411.a.B3.GE39985	globular domain (aln H.pyl struc)		190	482

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiafA.18411.a.B2.PS38258	soluble domain		58	482
RiafA.18411.a.B3.PW37930	globular domain (aln H.pyl struc)		190	482

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|347255.3.peg.439
RefSeq:	WP_012719546.1
UniProt:	C3PMZ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAEEQNNNHN TGSLSGVDSP EVQRELSKVS VSFNKSIVIV VVICGIFIYI FYTLFFGTAK EEIPETQIPS NIVKPVTEVD DNIPEIPKLP DPPKLETPTA PPPPPPSVVK VPPVLPPTTP VEENKDKTLP LPPVSLPSTS GTLVESNAEG QRREAKRKSA IVLIGGVEPK KTPEQITAET TFKDRGDMSL VLGRGKLIDA VLETAINSDL GGEIRAIISR DVFSEKDKVI LIPKGSKIFG KYATSTSSDS YGRVSIIWDR IDLTSGYTIE FDSPAVDNLG RPGLQGRVDN KYKEQFANAV LQSGFNIGLA KVLDKLVPPP IASQAAATNS ATAKQLLNTA QTIASNTAMD ANTRIVTICT NILAAITDKT STAYTTMTQA CTTAQTASSA NTAEQRLQTL VQAVNTAASS LLTTTSIAST PTQAQQASTQ AFTDVTNVVK NMITQQQFKP TTTVNQGTPV RIYVNKDYKF PKAVLLKSKV MK

NT Sequence

atggctgaag agcaaaataa caatcataat accggttctt tatcaggagt agattctcct gaagtacaaa gagaattatc aaaagtttcg gttagcttta ataagagtat tgtgatagta gtagtaattt gtggtatttt tatatatatt ttttatactc tttttttcgg tactgcaaaa gaagaaatac cggagactca gataccgagt aatattgtaa aacctgttac ggaggttgat gataatattc cggagattcc aaagttacct gatccgccta agcttgagac gcccaccgct cctccaccgc ctcctccttc ggtagtaaaa gttccaccgg tcttacctcc tactactcct gtagaagaga ataaagacaa aacgcttcca ttgccgccgg tttcattgcc ttcgacttca ggaacgttag ttgagagcaa tgcagagggg caacgtcgtg aagcaaaaag aaaatcagct atagtgttaa ttggtggtgt agagcctaaa aaaacaccgg aacagattac ggcagaaacg acctttaaag atcgcggtga tatgtctttg gttctcggac gcggtaaatt aattgatgca gttcttgaga cggcaataaa tagtgatctc ggcggtgaaa taagagccat tattagtaga gacgtatttt ctgagaaaga taaagtgata ttgataccga aagggtcaaa aatattcggt aaatatgcaa cctcaacttc gtccgatagt tatggtagag tttctataat atgggataga atcgatttaa cgagcggtta tactatagaa ttcgactcac ctgcagttga taatttaggt agaccgggat tacaaggaag agttgataat aaatataaag aacaatttgc aaatgccgta ttacaatccg gctttaatat agggcttgct aaagtgcttg ataagttagt gccgcctcct atagcttctc aggcagcggc taccaatagt gctacggcaa aacaattgtt aaatactgct caaactattg cgtctaatac cgctatggat gcaaatacta ggatagttac aatttgtact aatatacttg ctgctattac cgataaaact tcgactgctt atactactat gactcaagca tgtacaaccg cacaaactgc ttcttcagcg aatactgctg agcagagact tcaaacctta gtacaggcag ttaatacggc agcttcaagt ttgcttacta ctacctctat agcatcaact ccgactcaag cacaacaagc ttctactcaa gcttttacgg atgtaacgaa tgttgtaaaa aatatgataa ctcagcagca gtttaagccg acgacaacgg ttaatcaagg tacgccggtt agaatatacg ttaataagga ttataaattc ccaaaagccg ttttattaaa atcgaaggta atgaaatga

Details for RiafA.18411.a.B2.GE39984

HARVESTED ON:	2/26/2016
SEQUENCED ON:	3/1/2016
EXPECTED MW:	46kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMT AKEEIPETQI PSNIVKPVTE VDDNIPEIPK LPDPPKLETP TAPPPPPPSV VKVPPVLPPT TPVEENKDKT LPLPPVSLPS TSGTLVESNA EGQRREAKRK SAIVLIGGVE PKKTPEQITA ETTFKDRGDM SLVLGRGKLI DAVLETAINS DLGGEIRAII SRDVFSEKDK VILIPKGSKI FGKYATSTSS DSYGRVSIIW DRIDLTNGYT IEFDSPAVDN LGRPGLQGRV DNKYKEQFAN AVLQSGFNIG LAKVLDKLVP PPIASQAAAT NSATAKQLLN TAQTIASNTA MDANTRIVTI CTNILAAITD KTSTAYTTMT QACTTAQTAS SANTAEQRLQ TLVQAVNTAA SSLLTTTSIA STPTQAQQAS TQAFTDVTNV VKNMITQQQF KPTTTVNQGT PVRIYVNKDY KF

Validated NT Sequence

tgggaattta taatccttat taacgtatat tctaaccggc gtaccttgat taaccgttgt cgtcggctta aactgctgct gagttatcat attttttaca acattcgtta catccgtaaa agcttgagta gaagcttgtt gtgcttgagt cggagttgat gctatagagg tagtagtaag caaacttgaa gctgccgtat taactgcctg tactaaggtt tgaagtctct gctcagcagt attcgctgaa gaagcagttt gtgcggttgt acatgcttga gtcatagtag tataagcagt cgaagtttta tcggtaatag cagcaagtat attagtacaa attgtaacta tcctagtatt tgcatccata gcggtattag acgcaatagt ttgagcagta tttaacaatt gttttgccgt agcactattg gtagccgctg cctgagaagc tataggaggc ggcactaact tatcaagcac tttagcaagc cctatattaa agccggattg taatacggca tttgcaaatt gttctttata tttattatca actcttcctt gtaatcccgg tctacctaaa ttatcaactg caggtgagtc gaattctata gtataaccgt tcgttaaatc gattctatcc catattatag aaactctacc ataactatcg gacgaagttg aggttgcata tttaccgaat atttttgacc ctttcggtat caatatcact ttatctttct cagaaaatac gtctctacta ataatggctc ttatttcacc gccgagatca ctatttattg ccgtctcaag aactgcatca attaatttac cgcgtccgag aaccaaagac atatcaccgc gatctttaaa ggtcgtttct gccgtaatct gttccggtgt ttttttaggc tctacaccac caattaacac tatagctgat tttctttttg cttcacgacg ttgcccctct gcattgctct caactaacgt tcctgaagtc gaaggcaatg aaaccggcgg caatggaagc gttttgtctt tattctcttc tacaggagta gtaggaggta agaccggtgg aacttttact accgaaggag gaggcggtgg aggagcggtg ggcgtctcaa gcttaggcgg atcaggtaac tttggaatct ccggaatatt atcatcaacc tccgtaacag gttttacaat attactcggt atctgagtct ccggtatttc ttcttttgca gtcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHTA KEEIPETQIP SNIVKPVTEV DDNIPEIPKL PDPPKLETPT APPPPPPSVV KVPPVLPPTT PVEENKDKTL PLPPVSLPST SGTLVESNAE GQRREAKRKS AIVLIGGVEP KKTPEQITAE TTFKDRGDMS LVLGRGKLID AVLETAINSD LGGEIRAIIS RDVFSEKDKV ILIPKGSKIF GKYATSTSSD SYGRVSIIWD RIDLTSGYTI EFDSPAVDNL GRPGLQGRVD NKYKEQFANA VLQSGFNIGL AKVLDKLVPP PIASQAAATN SATAKQLLNT AQTIASNTAM DANTRIVTIC TNILAAITDK TSTAYTTMTQ ACTTAQTASS ANTAEQRLQT LVQAVNTAAS SLLTTTSIAS TPTQAQQAST QAFTDVTNVV KNMITQQQFK PTTTVNQGTP VRIYVNKDYK FPKAVLLKSK VMK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catactgcaa aagaagaaat accggagact cagataccga gtaatattgt aaaacctgtt acggaggttg atgataatat tccggagatt ccaaagttac ctgatccgcc taagcttgag acgcccaccg ctcctccacc gcctcctcct tcggtagtaa aagttccacc ggtcttacct cctactactc ctgtagaaga gaataaagac aaaacgcttc cattgccgcc ggtttcattg ccttcgactt caggaacgtt agttgagagc aatgcagagg ggcaacgtcg tgaagcaaaa agaaaatcag ctatagtgtt aattggtggt gtagagccta aaaaaacacc ggaacagatt acggcagaaa cgacctttaa agatcgcggt gatatgtctt tggttctcgg acgcggtaaa ttaattgatg cagttcttga gacggcaata aatagtgatc tcggcggtga aataagagcc attattagta gagacgtatt ttctgagaaa gataaagtga tattgatacc gaaagggtca aaaatattcg gtaaatatgc aacctcaact tcgtccgata gttatggtag agtttctata atatgggata gaatcgattt aacgagcggt tatactatag aattcgactc acctgcagtt gataatttag gtagaccggg attacaagga agagttgata ataaatataa agaacaattt gcaaatgccg tattacaatc cggctttaat atagggcttg ctaaagtgct tgataagtta gtgccgcctc ctatagcttc tcaggcagcg gctaccaata gtgctacggc aaaacaattg ttaaatactg ctcaaactat tgcgtctaat accgctatgg atgcaaatac taggatagtt acaatttgta ctaatatact tgctgctatt accgataaaa cttcgactgc ttatactact atgactcaag catgtacaac cgcacaaact gcttcttcag cgaatactgc tgagcagaga cttcaaacct tagtacaggc agttaatacg gcagcttcaa gtttgcttac tactacctct atagcatcaa ctccgactca agcacaacaa gcttctactc aagcttttac ggatgtaacg aatgttgtaa aaaatatgat aactcagcag cagtttaagc cgacgacaac ggttaatcaa ggtacgccgg ttagaatata cgttaataag gattataaat tcccaaaagc cgttttatta aaatcgaagg taatgaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for RiafA.18411.a.B3.GE39985

HARVESTED ON:	2/26/2016
SEQUENCED ON:	3/1/2016
EXPECTED MW:	32kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML VLGRGKLIDA VLETAINSDL GGEIRAIISR DVFSEKDKVI LIPKGSKIFG KYATSTSSDS YGRVSIIWDR IDLTNGYTIE FDSPAVDNLG RPGLQGRVDN KYKEQFANAV LQSGFNIGLA KVLDKLVPPP IASQAAATNS ATAKQLLNTA QTIASNTAMD ANTRIVTICT NILAAITDKT STAYTTMTQA CTTAQTASSA NTAEQRLQTL VQAVNTAASS LLTTTSIAST PTQAQQASTQ AFTDVTNVVK NMITQQQFKP TTTVNQGTPV RIYVNKDYKF PKAXXXKSKV MK

Validated NT Sequence

tcttactcac ttatttcatt accttcgatt tnnnnnaann ggcttttggg aatttataat ccttattaac gtatattcta accggcgtac cttgattaac cgttgtcgtc ggcttaaact gctgctgagt tatcatattt tttacaacat tcgttacatc cgtaaaagct tgagtagaag cttgttgtgc ttgagtcgga gttgatgcta tagaggtagt agtaagcaaa cttgaagctg ccgtattaac tgcctgtact aaggtttgaa gtctctgctc agcagtattc gctgaagaag cagtttgtgc ggttgtacat gcttgagtca tagtagtata agcagtcgaa gttttatcgg taatagcagc aagtatatta gtacaaattg taactatcct agtatttgca tccatagcgg tattagacgc aatagtttga gcagtattta acaattgttt tgccgtagca ctattggtag ccgctgcctg agaagctata ggaggcggca ctaacttatc aagcacttta gcaagcccta tattaaagcc ggattgtaat acggcatttg caaattgttc tttatattta ttatcaactc ttccttgtaa tcccggtcta cctaaattat caactgcagg tgagtcgaat tctatagtat aaccgttcgt taaatcgatt ctatcccata ttatagaaac tctaccataa ctatcggacg aagttgaggt tgcatattta ccgaatattt ttgacccttt cggtatcaat atcactttat ctttctcaga aaatacgtct ctactaataa tggctcttat ttcaccgccg agatcactat ttattgccgt ctcaagaact gcatcaatta atttaccgcg tccgagaacc aacatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHLV LGRGKLIDAV LETAINSDLG GEIRAIISRD VFSEKDKVIL IPKGSKIFGK YATSTSSDSY GRVSIIWDRI DLTSGYTIEF DSPAVDNLGR PGLQGRVDNK YKEQFANAVL QSGFNIGLAK VLDKLVPPPI ASQAAATNSA TAKQLLNTAQ TIASNTAMDA NTRIVTICTN ILAAITDKTS TAYTTMTQAC TTAQTASSAN TAEQRLQTLV QAVNTAASSL LTTTSIASTP TQAQQASTQA FTDVTNVVKN MITQQQFKPT TTVNQGTPVR IYVNKDYKFP KAVLLKSKVM K

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggttc tcggacgcgg taaattaatt gatgcagttc ttgagacggc aataaatagt gatctcggcg gtgaaataag agccattatt agtagagacg tattttctga gaaagataaa gtgatattga taccgaaagg gtcaaaaata ttcggtaaat atgcaacctc aacttcgtcc gatagttatg gtagagtttc tataatatgg gatagaatcg atttaacgag cggttatact atagaattcg actcacctgc agttgataat ttaggtagac cgggattaca aggaagagtt gataataaat ataaagaaca atttgcaaat gccgtattac aatccggctt taatataggg cttgctaaag tgcttgataa gttagtgccg cctcctatag cttctcaggc agcggctacc aatagtgcta cggcaaaaca attgttaaat actgctcaaa ctattgcgtc taataccgct atggatgcaa atactaggat agttacaatt tgtactaata tacttgctgc tattaccgat aaaacttcga ctgcttatac tactatgact caagcatgta caaccgcaca aactgcttct tcagcgaata ctgctgagca gagacttcaa accttagtac aggcagttaa tacggcagct tcaagtttgc ttactactac ctctatagca tcaactccga ctcaagcaca acaagcttct actcaagctt ttacggatgt aacgaatgtt gtaaaaaata tgataactca gcagcagttt aagccgacga caacggttaa tcaaggtacg ccggttagaa tatacgttaa taaggattat aaattcccaa aagccgtttt attaaaatcg aaggtaatga aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for RiafA.18411.a.B2.PS38258

PURIFICATION DATe:	4/18/2017
CONCENTRATION:	11.6mg/ml
OBSERVED MW:	55kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer: 20mM HEPES pH 7.0, 300mM NaCl, 5%glycerol, 1m MTCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATACTGCAA AAGAAGAAAT ACCGGAGACT CAGATACCGA GTAATATTGT AAAACCTGTT ACGGAGGTTG ATGATAATAT TCCGGAGATT CCAAAGTTAC CTGATCCGCC TAAGCTTGAG ACGCCCACCG CTCCTCCACC GCCTCCTCCT TCGGTAGTAA AAGTTCCACC GGTCTTACCT CCTACTACTC CTGTAGAAGA GAATAAAGAC AAAACGCTTC CATTGCCGCC GGTTTCATTG CCTTCGACTT CAGGAACGTT AGTTGAGAGC AATGCAGAGG GGCAACGTCG TGAAGCAAAA AGAAAATCAG CTATAGTGTT AATTGGTGGT GTAGAGCCTA AAAAAACACC GGAACAGATT ACGGCAGAAA CGACCTTTAA AGATCGCGGT GATATGTCTT TGGTTCTCGG ACGCGGTAAA TTAATTGATG CAGTTCTTGA GACGGCAATA AATAGTGATC TCGGCGGTGA AATAAGAGCC ATTATTAGTA GAGACGTATT TTCTGAGAAA GATAAAGTGA TATTGATACC GAAAGGGTCA AAAATATTCG GTAAATATGC AACCTCAACT TCGTCCGATA GTTATGGTAG AGTTTCTATA ATATGGGATA GAATCGATTT AACGAGCGGT TATACTATAG AATTCGACTC ACCTGCAGTT GATAATTTAG GTAGACCGGG ATTACAAGGA AGAGTTGATA ATAAATATAA AGAACAATTT GCAAATGCCG TATTACAATC CGGCTTTAAT ATAGGGCTTG CTAAAGTGCT TGATAAGTTA GTGCCGCCTC CTATAGCTTC TCAGGCAGCG GCTACCAATA GTGCTACGGC AAAACAATTG TTAAATACTG CTCAAACTAT TGCGTCTAAT ACCGCTATGG ATGCAAATAC TAGGATAGTT ACAATTTGTA CTAATATACT TGCTGCTATT ACCGATAAAA CTTCGACTGC TTATACTACT ATGACTCAAG CATGTACAAC CGCACAAACT GCTTCTTCAG CGAATACTGC TGAGCAGAGA CTTCAAACCT TAGTACAGGC AGTTAATACG GCAGCTTCAA GTTTGCTTAC TACTACCTCT ATAGCATCAA CTCCGACTCA AGCACAACAA GCTTCTACTC AAGCTTTTAC GGATGTAACG AATGTTGTAA AAAATATGAT AACTCAGCAG CAGTTTAAGC CGACGACAAC GGTTAATCAA GGTACGCCGG TTAGAATATA CGTTAATAAG GATTATAAAT TCCCAAAAGC CGTTTTATTA AAATCGAAGG TAATGAAATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT

Details for RiafA.18411.a.B3.PW37930

PURIFICATION DATe:	7/12/2016
CONCENTRATION:	22mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGGTTC TCGGACGCGG TAAATTAATT GATGCAGTTC TTGAGACGGC AATAAATAGT GATCTCGGCG GTGAAATAAG AGCCATTATT AGTAGAGACG TATTTTCTGA GAAAGATAAA GTGATATTGA TACCGAAAGG GTCAAAAATA TTCGGTAAAT ATGCAACCTC AACTTCGTCC GATAGTTATG GTAGAGTTTC TATAATATGG GATAGAATCG ATTTAACGAG CGGTTATACT ATAGAATTCG ACTCACCTGC AGTTGATAAT TTAGGTAGAC CGGGATTACA AGGAAGAGTT GATAATAAAT ATAAAGAACA ATTTGCAAAT GCCGTATTAC AATCCGGCTT TAATATAGGG CTTGCTAAAG TGCTTGATAA GTTAGTGCCG CCTCCTATAG CTTCTCAGGC AGCGGCTACC AATAGTGCTA CGGCAAAACA ATTGTTAAAT ACTGCTCAAA CTATTGCGTC TAATACCGCT ATGGATGCAA ATACTAGGAT AGTTACAATT TGTACTAATA TACTTGCTGC TATTACCGAT AAAACTTCGA CTGCTTATAC TACTATGACT CAAGCATGTA CAACCGCACA AACTGCTTCT TCAGCGAATA CTGCTGAGCA GAGACTTCAA ACCTTAGTAC AGGCAGTTAA TACGGCAGCT TCAAGTTTGC TTACTACTAC CTCTATAGCA TCAACTCCGA CTCAAGCACA ACAAGCTTCT ACTCAAGCTT TTACGGATGT AACGAATGTT GTAAAAAATA TGATAACTCA GCAGCAGTTT AAGCCGACGA CAACGGTTAA TCAAGGTACG CCGGTTAGAA TATACGTTAA TAAGGATTAT AAATTCCCAA AAGCCGTTTT ATTAAAATCG AAGGTAATGA AATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT