RiafA.17834.a

Putative methyltransferase

CENTER ID: RiafA.17834.a
ORGANISM: Rickettsia africae ESF-5
ASSOCIATED DISEASE: African tick-bite fever
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiafA.17834.a.B1.GE35899 Full length( RiafA.17834.a ) 1 554
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiafA.17834.a.B1.PS01705 Full length( RiafA.17834.a ) 1 554
RiafA.17834.a.B1.PS01706 Full length( RiafA.17834.a ) 1 554

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|347255.3.peg.1369
RefSeq: YP_002845651.1
UniProt: C3PLR8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSPKATNSSS TPNGHDKMAK KTHSAQSVVN GAVSDHNTYD EIPYESYPYA LTNPYHLSTL ATLFGVNAPE VENAKILELG CAAGGNLIPH AVLYPKAYFV GIDLSKVQID EANKNVKALG LKNIEFHHCS ITDINDSFGK FDYIICHGVI SWVPKTVRDK IFEVCNKNLS PNGIAYISYN TLPGWNMVRT IRDMMMYHSS SFANVRDRIA QSRLLLEFVK DSLENSKTPY AETLKTEAGL LAKQTDHYLR HDHLEEENAQ FYFHEFMNEA RKHNLQYLAD CNLSTMYLGN MPPKVVEQLK AVNDIVRTEQ YMDFITNRRF RTTLLCHSDV KINRNINNDD ITKFNIIFNI VPEKPLKEVD LNNASENLKF FLNGNQDSNL TTSSPYMKAI LYTFSENLNN PLSFEKITTE ANKKLHNTKL NEIKAELLNN AMKLVLQGYI SITNQKHRNN PELDKPKTTK MVIHQATHTP SMWVTNLQHE PIGVNFFEKF ALRYMDGKHD KKAIIEAVLG HVEKGELTLS KEGQKVENKE EIRKELESLF IPMIKKFSSN ALLV
NT Sequence
ATGTCTCCAA AAGCTACTAA CTCTTCTTCT ACCCCTAACG GTCATGATAA GATGGCAAAA AAAACACATT CCGCACAATC CGTTGTTAAC GGTGCGGTTT CAGATCATAA CACTTATGAT GAAATACCGT ATGAAAGCTA CCCATATGCT CTTACAAATC CTTATCACTT AAGTACACTT GCAACTCTTT TCGGTGTAAA TGCTCCTGAA GTCGAGAATG CAAAAATATT AGAGCTTGGT TGTGCAGCAG GCGGTAATTT AATACCACAT GCAGTTCTTT ATCCAAAAGC TTATTTTGTT GGGATTGATT TGTCTAAGGT ACAAATTGAC GAAGCAAATA AAAATGTTAA AGCACTAGGA TTAAAAAATA TAGAGTTCCA TCATTGTTCG ATAACTGATA TTAACGATTC TTTCGGTAAG TTTGATTATA TAATTTGCCA TGGCGTAATT TCTTGGGTAC CAAAAACCGT TAGAGATAAA ATTTTTGAAG TTTGTAATAA AAATCTTAGT CCAAACGGAA TAGCATATAT TAGCTATAAT ACCCTCCCTG GCTGGAATAT GGTCCGTACT ATTAGAGATA TGATGATGTA TCATTCTAGC TCATTTGCAA ATGTACGGGA TAGAATAGCT CAATCTAGAT TGTTACTAGA ATTTGTTAAG GATAGCTTAG AAAACTCTAA AACTCCTTAT GCAGAAACAT TAAAAACCGA AGCAGGGCTA CTTGCTAAAC AAACCGATCA TTATTTACGT CATGATCATC TAGAAGAAGA AAATGCTCAA TTCTACTTCC ATGAATTTAT GAATGAAGCA AGAAAGCACA ATTTACAATA TTTAGCCGAT TGTAATCTTT CGACTATGTA TCTCGGTAAT ATGCCGCCGA AAGTAGTAGA GCAGTTAAAA GCGGTAAACG ATATAGTTAG AACCGAACAA TATATGGATT TCATTACGAA TCGTCGTTTT AGAACCACTT TACTATGTCA TAGTGATGTG AAAATTAATA GAAATATTAA CAATGATGAT ATAACGAAAT TTAATATAAT CTTTAATATA GTGCCTGAAA AACCACTCAA AGAAGTAGAT CTTAATAATG CTTCCGAAAA TTTAAAATTC TTCTTAAACG GCAATCAAGA CTCTAACTTA ACAACTAGTT CGCCTTATAT GAAAGCTATT TTATACACTT TCAGCGAAAA TCTAAATAAT CCGTTAAGCT TTGAGAAAAT AACCACTGAA GCTAATAAAA AGCTACATAA TACTAAATTA AACGAAATCA AAGCTGAGCT TTTAAATAAT GCAATGAAGC TAGTACTACA AGGTTATATT AGTATTACAA ATCAGAAGCA TCGAAATAAC CCTGAGCTTG ATAAGCCTAA AACAACAAAA ATGGTGATAC ATCAAGCAAC CCATACACCT TCTATGTGGG TTACAAATTT ACAACATGAA CCGATCGGCG TTAATTTCTT TGAGAAGTTT GCACTTAGAT ATATGGATGG TAAACATGAT AAAAAAGCAA TCATTGAGGC TGTCCTTGGT CATGTAGAAA AAGGCGAGCT AACTTTAAGT AAAGAAGGTC AAAAAGTAGA AAACAAGGAA GAAATACGCA AAGAACTAGA AAGCTTATTT ATTCCGATGA TCAAGAAATT TTCTTCTAAT GCTTTGTTGG TG
Details for RiafA.17834.a.B1.GE35899
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 64kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 58
Validated AA Sequence
MAKKTHSAQS VVNGAVSDHN TYDEIPYESY PYALTNPYHL STLATLFGVN APEVENAKIL ELGCAAGGNL IPHAVLYPKA YFVGIDLSKV QIDEANKNVK ALGLKNIEFH HCSITDINDS FGKFDYIICH GVISWVPKTV RDKIFEVCNK NLSPNGIAYI SYNTLPGWNM VRTIRDMMMY HSSSFANVRD RIAQSRLLLE FVKDSLENSK TPYAETLKTE AXLLAKQTDH YLRHDHLEEE NAQFYFHEFM NEARKHNLQY LADCNLSTMY LGNMPPKVVE QLKAVNDIVR TEQYMDFITN RRFRTTLLCH SDVKINRNXN NDDITXF
Validated NT Sequence
agaagaaaat ttcttgatca tcggaataaa taagctttct agttctttgc gtatttcttc cttgttttct actttttgac cttctttact taaagttagc tcgccttttt ctacatgacc aaggacagcc tcaatgattg cttttttatc atgtttacca tccatatatc taagtgcaaa cttctcaaag aaattaacgc cgatcggttc atgttgtaaa tttgtaaccc acatagaagg tgtatgggtt gcttgatgta tcaccatttt tgttgtttta ggcttatcaa gctcagggtt atttcgatgc ttctgatttg taatactaat ataaccttgt agtactagct tcattgcatt atttaaaagc tcagctttga tttcgtttaa tttagtatta tgtagctttt tattagcttc agtggttatt ttctcaaagc ttaacggatt atttagattt tcgctgaaag tgtataaaat agctttcata taaggcgaac tagttgttaa gttagagtct tgattgccgt ttaagaagaa ttttaaattt tcggaagcat tattaagatc tacttctttg agtggttttt caggcactat attaaagatt atattaaaan ttcgttatat catcattgtt natatttcta ttaattttca catcactatg acatagtaaa gtggttctaa aacgacgatt cgtaatgaaa tccatatatt gttcggttct aactatatcg tttaccgctt ttaactgctc tactactttc ggcggcatat taccgagata catagtcgaa agattacaat cggctaaata ttgtaaattg tgctttcttg cttcattcat aaattcatgg aagtagaatt gagcattttc ttcttctaga tgatcatgac gtaaataatg atcggtttgt ttagcaagta gccntgcttc ggtttttaat gtttctgcat aaggagtttt agagttttct aagctatcct taacaaattc tagtaacaat ctagattgag ctattctatc ccgtacattt gcaaatgagc tagaatgata catcatcata tctctaatag tacggaccat attccagcca gggagggtat tatagctaat atatgctatt ccgtttggac taagattttt attacaaact tcaaaaattt tatctctaac ggtttttggt acccaagaaa ttacgccatg gcaaattata taatcaaact taccgaaaga atcgttaata tcagttatcg aacaatgatg gaactctata ttttttaatc ctagtgcttt aacattttta tttgcttcgt caatttgtac cttagacaaa tcaatcccaa caaaataagc ttttggataa agaactgcat gtggtattaa attaccgcct gctgcacaac caagctctaa tatttttgca ttctcgactt caggagcatt tacaccgaaa agagttgcaa gtgtacttaa gtgataagga tttgtaagag catatgggta gctttcatac ggtatttcat cataagtgtt atgatctgaa accgcaccgt taacaacgga ttgtgcggaa tgtgtttttt ttgccatctt atcatgaccg ttaggggtag aagaagagtt agtagctt
Expected Protein Sequence
MAHHHHHHMS PKATNSSSTP NGHDKMAKKT HSAQSVVNGA VSDHNTYDEI PYESYPYALT NPYHLSTLAT LFGVNAPEVE NAKILELGCA AGGNLIPHAV LYPKAYFVGI DLSKVQIDEA NKNVKALGLK NIEFHHCSIT DINDSFGKFD YIICHGVISW VPKTVRDKIF EVCNKNLSPN GIAYISYNTL PGWNMVRTIR DMMMYHSSSF ANVRDRIAQS RLLLEFVKDS LENSKTPYAE TLKTEAGLLA KQTDHYLRHD HLEEENAQFY FHEFMNEARK HNLQYLADCN LSTMYLGNMP PKVVEQLKAV NDIVRTEQYM DFITNRRFRT TLLCHSDVKI NRNINNDDIT KFNIIFNIVP EKPLKEVDLN NASENLKFFL NGNQDSNLTT SSPYMKAILY TFSENLNNPL SFEKITTEAN KKLHNTKLNE IKAELLNNAM KLVLQGYISI TNQKHRNNPE LDKPKTTKMV IHQATHTPSM WVTNLQHEPI GVNFFEKFAL RYMDGKHDKK AIIEAVLGHV EKGELTLSKE GQKVENKEEI RKELESLFIP MIKKFSSNAL LV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctc caaaagctac taactcttct tctaccccta acggtcatga taagatggca aaaaaaacac attccgcaca atccgttgtt aacggtgcgg tttcagatca taacacttat gatgaaatac cgtatgaaag ctacccatat gctcttacaa atccttatca cttaagtaca cttgcaactc ttttcggtgt aaatgctcct gaagtcgaga atgcaaaaat attagagctt ggttgtgcag caggcggtaa tttaatacca catgcagttc tttatccaaa agcttatttt gttgggattg atttgtctaa ggtacaaatt gacgaagcaa ataaaaatgt taaagcacta ggattaaaaa atatagagtt ccatcattgt tcgataactg atattaacga ttctttcggt aagtttgatt atataatttg ccatggcgta atttcttggg taccaaaaac cgttagagat aaaatttttg aagtttgtaa taaaaatctt agtccaaacg gaatagcata tattagctat aataccctcc ctggctggaa tatggtccgt actattagag atatgatgat gtatcattct agctcatttg caaatgtacg ggatagaata gctcaatcta gattgttact agaatttgtt aaggatagct tagaaaactc taaaactcct tatgcagaaa cattaaaaac cgaagcaggg ctacttgcta aacaaaccga tcattattta cgtcatgatc atctagaaga agaaaatgct caattctact tccatgaatt tatgaatgaa gcaagaaagc acaatttaca atatttagcc gattgtaatc tttcgactat gtatctcggt aatatgccgc cgaaagtagt agagcagtta aaagcggtaa acgatatagt tagaaccgaa caatatatgg atttcattac gaatcgtcgt tttagaacca ctttactatg tcatagtgat gtgaaaatta atagaaatat taacaatgat gatataacga aatttaatat aatctttaat atagtgcctg aaaaaccact caaagaagta gatcttaata atgcttccga aaatttaaaa ttcttcttaa acggcaatca agactctaac ttaacaacta gttcgcctta tatgaaagct attttataca ctttcagcga aaatctaaat aatccgttaa gctttgagaa aataaccact gaagctaata aaaagctaca taatactaaa ttaaacgaaa tcaaagctga gcttttaaat aatgcaatga agctagtact acaaggttat attagtatta caaatcagaa gcatcgaaat aaccctgagc ttgataagcc taaaacaaca aaaatggtga tacatcaagc aacccataca ccttctatgt gggttacaaa tttacaacat gaaccgatcg gcgttaattt ctttgagaag tttgcactta gatatatgga tggtaaacat gataaaaaag caatcattga ggctgtcctt ggtcatgtag aaaaaggcga gctaacttta agtaaagaag gtcaaaaagt agaaaacaag gaagaaatac gcaaagaact agaaagctta tttattccga tgatcaagaa attttcttct aatgctttgt tggtgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for RiafA.17834.a.B1.PS01705
PURIFICATION DATe: 12/12/2012
CONCENTRATION: 12.7mg/ml
OBSERVED MW: 66kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 58
Protocol Notes
notes unavailable
Validated AA Sequence
MAKKTHSAQS VVNGAVSDHN TYDEIPYESY PYALTNPYHL STLATLFGVN APEVENAKIL ELGCAAGGNL IPHAVLYPKA YFVGIDLSKV QIDEANKNVK ALGLKNIEFH HCSITDINDS FGKFDYIICH GVISWVPKTV RDKIFEVCNK NLSPNGIAYI SYNTLPGWNM VRTIRDMMMY HSSSFANVRD RIAQSRLLLE FVKDSLENSK TPYAETLKTE AXLLAKQTDH YLRHDHLEEE NAQFYFHEFM NEARKHNLQY LADCNLSTMY LGNMPPKVVE QLKAVNDIVR TEQYMDFITN RRFRTTLLCH SDVKINRNXN NDDITXF
Validated NT Sequence
agaagaaaat ttcttgatca tcggaataaa taagctttct agttctttgc gtatttcttc cttgttttct actttttgac cttctttact taaagttagc tcgccttttt ctacatgacc aaggacagcc tcaatgattg cttttttatc atgtttacca tccatatatc taagtgcaaa cttctcaaag aaattaacgc cgatcggttc atgttgtaaa tttgtaaccc acatagaagg tgtatgggtt gcttgatgta tcaccatttt tgttgtttta ggcttatcaa gctcagggtt atttcgatgc ttctgatttg taatactaat ataaccttgt agtactagct tcattgcatt atttaaaagc tcagctttga tttcgtttaa tttagtatta tgtagctttt tattagcttc agtggttatt ttctcaaagc ttaacggatt atttagattt tcgctgaaag tgtataaaat agctttcata taaggcgaac tagttgttaa gttagagtct tgattgccgt ttaagaagaa ttttaaattt tcggaagcat tattaagatc tacttctttg agtggttttt caggcactat attaaagatt atattaaaan ttcgttatat catcattgtt natatttcta ttaattttca catcactatg acatagtaaa gtggttctaa aacgacgatt cgtaatgaaa tccatatatt gttcggttct aactatatcg tttaccgctt ttaactgctc tactactttc ggcggcatat taccgagata catagtcgaa agattacaat cggctaaata ttgtaaattg tgctttcttg cttcattcat aaattcatgg aagtagaatt gagcattttc ttcttctaga tgatcatgac gtaaataatg atcggtttgt ttagcaagta gccntgcttc ggtttttaat gtttctgcat aaggagtttt agagttttct aagctatcct taacaaattc tagtaacaat ctagattgag ctattctatc ccgtacattt gcaaatgagc tagaatgata catcatcata tctctaatag tacggaccat attccagcca gggagggtat tatagctaat atatgctatt ccgtttggac taagattttt attacaaact tcaaaaattt tatctctaac ggtttttggt acccaagaaa ttacgccatg gcaaattata taatcaaact taccgaaaga atcgttaata tcagttatcg aacaatgatg gaactctata ttttttaatc ctagtgcttt aacattttta tttgcttcgt caatttgtac cttagacaaa tcaatcccaa caaaataagc ttttggataa agaactgcat gtggtattaa attaccgcct gctgcacaac caagctctaa tatttttgca ttctcgactt caggagcatt tacaccgaaa agagttgcaa gtgtacttaa gtgataagga tttgtaagag catatgggta gctttcatac ggtatttcat cataagtgtt atgatctgaa accgcaccgt taacaacgga ttgtgcggaa tgtgtttttt ttgccatctt atcatgaccg ttaggggtag aagaagagtt agtagctt
Expressed Protein Sequence
MAHHHHHHMS PKATNSSSTP NGHDKMAKKT HSAQSVVNGA VSDHNTYDEI PYESYPYALT NPYHLSTLAT LFGVNAPEVE NAKILELGCA AGGNLIPHAV LYPKAYFVGI DLSKVQIDEA NKNVKALGLK NIEFHHCSIT DINDSFGKFD YIICHGVISW VPKTVRDKIF EVCNKNLSPN GIAYISYNTL PGWNMVRTIR DMMMYHSSSF ANVRDRIAQS RLLLEFVKDS LENSKTPYAE TLKTEAGLLA KQTDHYLRHD HLEEENAQFY FHEFMNEARK HNLQYLADCN LSTMYLGNMP PKVVEQLKAV NDIVRTEQYM DFITNRRFRT TLLCHSDVKI NRNINNDDIT KFNIIFNIVP EKPLKEVDLN NASENLKFFL NGNQDSNLTT SSPYMKAILY TFSENLNNPL SFEKITTEAN KKLHNTKLNE IKAELLNNAM KLVLQGYISI TNQKHRNNPE LDKPKTTKMV IHQATHTPSM WVTNLQHEPI GVNFFEKFAL RYMDGKHDKK AIIEAVLGHV EKGELTLSKE GQKVENKEEI RKELESLFIP MIKKFSSNAL LV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTC CAAAAGCTAC TAACTCTTCT TCTACCCCTA ACGGTCATGA TAAGATGGCA AAAAAAACAC ATTCCGCACA ATCCGTTGTT AACGGTGCGG TTTCAGATCA TAACACTTAT GATGAAATAC CGTATGAAAG CTACCCATAT GCTCTTACAA ATCCTTATCA CTTAAGTACA CTTGCAACTC TTTTCGGTGT AAATGCTCCT GAAGTCGAGA ATGCAAAAAT ATTAGAGCTT GGTTGTGCAG CAGGCGGTAA TTTAATACCA CATGCAGTTC TTTATCCAAA AGCTTATTTT GTTGGGATTG ATTTGTCTAA GGTACAAATT GACGAAGCAA ATAAAAATGT TAAAGCACTA GGATTAAAAA ATATAGAGTT CCATCATTGT TCGATAACTG ATATTAACGA TTCTTTCGGT AAGTTTGATT ATATAATTTG CCATGGCGTA ATTTCTTGGG TACCAAAAAC CGTTAGAGAT AAAATTTTTG AAGTTTGTAA TAAAAATCTT AGTCCAAACG GAATAGCATA TATTAGCTAT AATACCCTCC CTGGCTGGAA TATGGTCCGT ACTATTAGAG ATATGATGAT GTATCATTCT AGCTCATTTG CAAATGTACG GGATAGAATA GCTCAATCTA GATTGTTACT AGAATTTGTT AAGGATAGCT TAGAAAACTC TAAAACTCCT TATGCAGAAA CATTAAAAAC CGAAGCAGGG CTACTTGCTA AACAAACCGA TCATTATTTA CGTCATGATC ATCTAGAAGA AGAAAATGCT CAATTCTACT TCCATGAATT TATGAATGAA GCAAGAAAGC ACAATTTACA ATATTTAGCC GATTGTAATC TTTCGACTAT GTATCTCGGT AATATGCCGC CGAAAGTAGT AGAGCAGTTA AAAGCGGTAA ACGATATAGT TAGAACCGAA CAATATATGG ATTTCATTAC GAATCGTCGT TTTAGAACCA CTTTACTATG TCATAGTGAT GTGAAAATTA ATAGAAATAT TAACAATGAT GATATAACGA AATTTAATAT AATCTTTAAT ATAGTGCCTG AAAAACCACT CAAAGAAGTA GATCTTAATA ATGCTTCCGA AAATTTAAAA TTCTTCTTAA ACGGCAATCA AGACTCTAAC TTAACAACTA GTTCGCCTTA TATGAAAGCT ATTTTATACA CTTTCAGCGA AAATCTAAAT AATCCGTTAA GCTTTGAGAA AATAACCACT GAAGCTAATA AAAAGCTACA TAATACTAAA TTAAACGAAA TCAAAGCTGA GCTTTTAAAT AATGCAATGA AGCTAGTACT ACAAGGTTAT ATTAGTATTA CAAATCAGAA GCATCGAAAT AACCCTGAGC TTGATAAGCC TAAAACAACA AAAATGGTGA TACATCAAGC AACCCATACA CCTTCTATGT GGGTTACAAA TTTACAACAT GAACCGATCG GCGTTAATTT CTTTGAGAAG TTTGCACTTA GATATATGGA TGGTAAACAT GATAAAAAAG CAATCATTGA GGCTGTCCTT GGTCATGTAG AAAAAGGCGA GCTAACTTTA AGTAAAGAAG GTCAAAAAGT AGAAAACAAG GAAGAAATAC GCAAAGAACT AGAAAGCTTA TTTATTCCGA TGATCAAGAA ATTTTCTTCT AATGCTTTGT TGGTGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT
Details for RiafA.17834.a.B1.PS01706
PURIFICATION DATe: 12/12/2012
CONCENTRATION: 14.8mg/ml
OBSERVED MW: 66kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 58
Protocol Notes
notes unavailable
Validated AA Sequence
MAKKTHSAQS VVNGAVSDHN TYDEIPYESY PYALTNPYHL STLATLFGVN APEVENAKIL ELGCAAGGNL IPHAVLYPKA YFVGIDLSKV QIDEANKNVK ALGLKNIEFH HCSITDINDS FGKFDYIICH GVISWVPKTV RDKIFEVCNK NLSPNGIAYI SYNTLPGWNM VRTIRDMMMY HSSSFANVRD RIAQSRLLLE FVKDSLENSK TPYAETLKTE AXLLAKQTDH YLRHDHLEEE NAQFYFHEFM NEARKHNLQY LADCNLSTMY LGNMPPKVVE QLKAVNDIVR TEQYMDFITN RRFRTTLLCH SDVKINRNXN NDDITXF
Validated NT Sequence
agaagaaaat ttcttgatca tcggaataaa taagctttct agttctttgc gtatttcttc cttgttttct actttttgac cttctttact taaagttagc tcgccttttt ctacatgacc aaggacagcc tcaatgattg cttttttatc atgtttacca tccatatatc taagtgcaaa cttctcaaag aaattaacgc cgatcggttc atgttgtaaa tttgtaaccc acatagaagg tgtatgggtt gcttgatgta tcaccatttt tgttgtttta ggcttatcaa gctcagggtt atttcgatgc ttctgatttg taatactaat ataaccttgt agtactagct tcattgcatt atttaaaagc tcagctttga tttcgtttaa tttagtatta tgtagctttt tattagcttc agtggttatt ttctcaaagc ttaacggatt atttagattt tcgctgaaag tgtataaaat agctttcata taaggcgaac tagttgttaa gttagagtct tgattgccgt ttaagaagaa ttttaaattt tcggaagcat tattaagatc tacttctttg agtggttttt caggcactat attaaagatt atattaaaan ttcgttatat catcattgtt natatttcta ttaattttca catcactatg acatagtaaa gtggttctaa aacgacgatt cgtaatgaaa tccatatatt gttcggttct aactatatcg tttaccgctt ttaactgctc tactactttc ggcggcatat taccgagata catagtcgaa agattacaat cggctaaata ttgtaaattg tgctttcttg cttcattcat aaattcatgg aagtagaatt gagcattttc ttcttctaga tgatcatgac gtaaataatg atcggtttgt ttagcaagta gccntgcttc ggtttttaat gtttctgcat aaggagtttt agagttttct aagctatcct taacaaattc tagtaacaat ctagattgag ctattctatc ccgtacattt gcaaatgagc tagaatgata catcatcata tctctaatag tacggaccat attccagcca gggagggtat tatagctaat atatgctatt ccgtttggac taagattttt attacaaact tcaaaaattt tatctctaac ggtttttggt acccaagaaa ttacgccatg gcaaattata taatcaaact taccgaaaga atcgttaata tcagttatcg aacaatgatg gaactctata ttttttaatc ctagtgcttt aacattttta tttgcttcgt caatttgtac cttagacaaa tcaatcccaa caaaataagc ttttggataa agaactgcat gtggtattaa attaccgcct gctgcacaac caagctctaa tatttttgca ttctcgactt caggagcatt tacaccgaaa agagttgcaa gtgtacttaa gtgataagga tttgtaagag catatgggta gctttcatac ggtatttcat cataagtgtt atgatctgaa accgcaccgt taacaacgga ttgtgcggaa tgtgtttttt ttgccatctt atcatgaccg ttaggggtag aagaagagtt agtagctt
Expressed Protein Sequence
MAHHHHHHMS PKATNSSSTP NGHDKMAKKT HSAQSVVNGA VSDHNTYDEI PYESYPYALT NPYHLSTLAT LFGVNAPEVE NAKILELGCA AGGNLIPHAV LYPKAYFVGI DLSKVQIDEA NKNVKALGLK NIEFHHCSIT DINDSFGKFD YIICHGVISW VPKTVRDKIF EVCNKNLSPN GIAYISYNTL PGWNMVRTIR DMMMYHSSSF ANVRDRIAQS RLLLEFVKDS LENSKTPYAE TLKTEAGLLA KQTDHYLRHD HLEEENAQFY FHEFMNEARK HNLQYLADCN LSTMYLGNMP PKVVEQLKAV NDIVRTEQYM DFITNRRFRT TLLCHSDVKI NRNINNDDIT KFNIIFNIVP EKPLKEVDLN NASENLKFFL NGNQDSNLTT SSPYMKAILY TFSENLNNPL SFEKITTEAN KKLHNTKLNE IKAELLNNAM KLVLQGYISI TNQKHRNNPE LDKPKTTKMV IHQATHTPSM WVTNLQHEPI GVNFFEKFAL RYMDGKHDKK AIIEAVLGHV EKGELTLSKE GQKVENKEEI RKELESLFIP MIKKFSSNAL LV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTC CAAAAGCTAC TAACTCTTCT TCTACCCCTA ACGGTCATGA TAAGATGGCA AAAAAAACAC ATTCCGCACA ATCCGTTGTT AACGGTGCGG TTTCAGATCA TAACACTTAT GATGAAATAC CGTATGAAAG CTACCCATAT GCTCTTACAA ATCCTTATCA CTTAAGTACA CTTGCAACTC TTTTCGGTGT AAATGCTCCT GAAGTCGAGA ATGCAAAAAT ATTAGAGCTT GGTTGTGCAG CAGGCGGTAA TTTAATACCA CATGCAGTTC TTTATCCAAA AGCTTATTTT GTTGGGATTG ATTTGTCTAA GGTACAAATT GACGAAGCAA ATAAAAATGT TAAAGCACTA GGATTAAAAA ATATAGAGTT CCATCATTGT TCGATAACTG ATATTAACGA TTCTTTCGGT AAGTTTGATT ATATAATTTG CCATGGCGTA ATTTCTTGGG TACCAAAAAC CGTTAGAGAT AAAATTTTTG AAGTTTGTAA TAAAAATCTT AGTCCAAACG GAATAGCATA TATTAGCTAT AATACCCTCC CTGGCTGGAA TATGGTCCGT ACTATTAGAG ATATGATGAT GTATCATTCT AGCTCATTTG CAAATGTACG GGATAGAATA GCTCAATCTA GATTGTTACT AGAATTTGTT AAGGATAGCT TAGAAAACTC TAAAACTCCT TATGCAGAAA CATTAAAAAC CGAAGCAGGG CTACTTGCTA AACAAACCGA TCATTATTTA CGTCATGATC ATCTAGAAGA AGAAAATGCT CAATTCTACT TCCATGAATT TATGAATGAA GCAAGAAAGC ACAATTTACA ATATTTAGCC GATTGTAATC TTTCGACTAT GTATCTCGGT AATATGCCGC CGAAAGTAGT AGAGCAGTTA AAAGCGGTAA ACGATATAGT TAGAACCGAA CAATATATGG ATTTCATTAC GAATCGTCGT TTTAGAACCA CTTTACTATG TCATAGTGAT GTGAAAATTA ATAGAAATAT TAACAATGAT GATATAACGA AATTTAATAT AATCTTTAAT ATAGTGCCTG AAAAACCACT CAAAGAAGTA GATCTTAATA ATGCTTCCGA AAATTTAAAA TTCTTCTTAA ACGGCAATCA AGACTCTAAC TTAACAACTA GTTCGCCTTA TATGAAAGCT ATTTTATACA CTTTCAGCGA AAATCTAAAT AATCCGTTAA GCTTTGAGAA AATAACCACT GAAGCTAATA AAAAGCTACA TAATACTAAA TTAAACGAAA TCAAAGCTGA GCTTTTAAAT AATGCAATGA AGCTAGTACT ACAAGGTTAT ATTAGTATTA CAAATCAGAA GCATCGAAAT AACCCTGAGC TTGATAAGCC TAAAACAACA AAAATGGTGA TACATCAAGC AACCCATACA CCTTCTATGT GGGTTACAAA TTTACAACAT GAACCGATCG GCGTTAATTT CTTTGAGAAG TTTGCACTTA GATATATGGA TGGTAAACAT GATAAAAAAG CAATCATTGA GGCTGTCCTT GGTCATGTAG AAAAAGGCGA GCTAACTTTA AGTAAAGAAG GTCAAAAAGT AGAAAACAAG GAAGAAATAC GCAAAGAACT AGAAAGCTTA TTTATTCCGA TGATCAAGAA ATTTTCTTCT AATGCTTTGT TGGTGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT