SSGCID: Target Page

RiafA.17612.a

Putative uncharacterized protein

CENTER ID:	RiafA.17612.a
ORGANISM:	Rickettsia africae ESF-5
ASSOCIATED DISEASE:	African tick-bite fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiafA.17612.a.B1.GE35896	Full length( RiafA.17612.a )		1	199

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|347255.3.peg.10
RefSeq:	YP_002844743.1
UniProt:	C3PM40

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MYKVKGQFLT IDMCPSSKSF EEDFFKKLLE LSIKLNKPIP IAICVSGLWI NKHTEEFLWL LKQQENGYLQ ITWVNHSFSH PYFKDKPLED NFLLSNKDDF ENEVLEAGKI LVSYNIAPSP FFRFPGLVSD QTLIEKLKDL GFIPLGSNAW LAKGEKVQNG SFILVHGNSN EKAGIDLIMP MLPELKLLPI EKTFLLHDN

NT Sequence

ATGTATAAAG TAAAAGGACA GTTCTTGACA ATTGACATGT GTCCTTCTTC CAAAAGCTTT GAAGAAGATT TTTTTAAAAA GCTCTTAGAA TTATCGATCA AACTAAATAA ACCTATTCCG ATTGCTATAT GTGTTTCAGG ATTATGGATT AATAAGCATA CAGAAGAATT CTTATGGTTA TTAAAACAAC AAGAAAACGG TTATTTACAA ATAACATGGG TTAATCATTC ATTTAGTCAT CCTTATTTTA AAGATAAACC TCTTGAAGAT AATTTCCTTC TTTCCAATAA AGATGATTTT GAAAATGAAG TATTAGAAGC AGGGAAAATA TTAGTAAGTT ATAATATTGC TCCGTCACCT TTCTTTCGTT TTCCTGGATT AGTTTCCGAT CAAACATTAA TAGAAAAATT AAAAGATTTG GGATTTATTC CGCTTGGGAG TAATGCATGG CTTGCTAAAG GCGAAAAAGT TCAAAATGGT TCTTTTATAT TGGTACACGG TAATAGTAAC GAAAAAGCCG GAATAGATTT AATTATGCCG ATGTTACCGG AATTAAAATT ACTTCCTATT GAAAAGACGT TTTTATTACA TGACAAT

Details for RiafA.17612.a.B1.GE35896

HARVESTED ON:	8/14/2012
SEQUENCED ON:	8/18/2012
EXPECTED MW:	23kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMY KVKGQFLTID MCPSSKSFEE DFFKKLVELS IKLNKPIPIA ICVSGLWINK HTEEFLWLLK QQENGYLQIT WVNHSFSHPY FKDKPLEDNF LLSNKDDFEN EVLEAGKILV SYNIAPSPFF RFPGLVSDQT LIEKLKDLGF IPLGSNAWLA KGEKVQNGSF ILVHGNSNEK AGIDLIMPML PELKLLPIEK TFLLHDN

Validated NT Sequence

ttgtttactt taagaaggag anataccatg gctcaccacc accaccacca tatgtataaa gtaaaaggac agttcttgac aattgacatg tgtccttctt ccaaaagctt tgaagaagat ttttttaaaa agctcgtaga attatcgatc aaactaaata aacctattcc gattgctata tgtgtttcag gattatggat taataagcat acagaagaat tcttatggtt attaaaacaa caagaaaacg gttatttaca aataacatgg gttaatcatt catttagtca tccttatttt aaagataaac ctcttgaaga taatttcctt ctttccaata aagatgattt tgaaaatgaa gtattagaag cagggaaaat attagtaagt tataatattg ctccgtcacc tttctttcgt tttcctggat tagtttccga tcaaacatta atagaaaaat taaaagattt gggatttatt ccgcttggga gtaatgcatg gcttgctaaa ggcgaaaaag ttcaaaatgg ttcttttata ttggtacacg gtaatagtaa cgaaaaagcc ggaatagatt taattatgcc gatgttaccg gaattaaaat tacttcctat tgaaaagacg tttttattac atgacaatta agtgagtaag ataggatccg gctgctaaca cccccnnnan gnannnnnnn nnnaaaanaa nnnnnnnnnn nntcagnnnn nnnnnnnnnc nnnnnnnnnn nnngnnanng nannnnnntn nannnnnnna ntcnnnnnnn nnngcnnnnn nnnnnnnnnn tanncnnncn nnacanngnt nnttnnang

Expected Protein Sequence

MAHHHHHHMY KVKGQFLTID MCPSSKSFEE DFFKKLLELS IKLNKPIPIA ICVSGLWINK HTEEFLWLLK QQENGYLQIT WVNHSFSHPY FKDKPLEDNF LLSNKDDFEN EVLEAGKILV SYNIAPSPFF RFPGLVSDQT LIEKLKDLGF IPLGSNAWLA KGEKVQNGSF ILVHGNSNEK AGIDLIMPML PELKLLPIEK TFLLHDN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtata aagtaaaagg acagttcttg acaattgaca tgtgtccttc ttccaaaagc tttgaagaag atttttttaa aaagctctta gaattatcga tcaaactaaa taaacctatt ccgattgcta tatgtgtttc aggattatgg attaataagc atacagaaga attcttatgg ttattaaaac aacaagaaaa cggttattta caaataacat gggttaatca ttcatttagt catccttatt ttaaagataa acctcttgaa gataatttcc ttctttccaa taaagatgat tttgaaaatg aagtattaga agcagggaaa atattagtaa gttataatat tgctccgtca cctttctttc gttttcctgg attagtttcc gatcaaacat taatagaaaa attaaaagat ttgggattta ttccgcttgg gagtaatgca tggcttgcta aaggcgaaaa agttcaaaat ggttctttta tattggtaca cggtaatagt aacgaaaaag ccggaataga tttaattatg ccgatgttac cggaattaaa attacttcct attgaaaaga cgtttttatt acatgacaat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat