SSGCID: Target Page

RiafA.17528.a

DNA repair protein RecN

CENTER ID:	RiafA.17528.a
ORGANISM:	Rickettsia africae ESF-5
ASSOCIATED DISEASE:	African tick-bite fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiafA.17528.a.B1.GE35895	Full length( RiafA.17528.a )		1	545

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|347255.3.peg.258
RefSeq:	YP_002844926.1
UniProt:	C3PMM3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFYSLSVKNF ILIDELEIEF NKGLCVITGE TGAGKSILLD AILFCLGYKT SNNIIKRGKD YAVVNILFSL NEEIKNFLVQ NLIEPEELLF VKCLQKAEGR KNFFINNQVV TKALMQQLAT YLFELHGQNN NISLLAANTQ RDILDSYGNL LDFRAELSNC YQAWQNTRKE IAAITLKQNS IEQEIDYLSF ATEELTKLNI QTGEEEQLAN LRKDLQNKDK DLQLIKDILE QINNPEINTS INRAEKLLAR QGNNERFEAI ITSLEEAYNN LEEARQELSN LLDSFNCEEY NLEETEERLF LIKAISRKYN VPADALGVFL DKSLEQLSIL KNKIANSNEL KAQEVLLQKK YYELASNLSA KRLIVAKHLE ESLHQELKQL KMAKAIFEIE INAKEPTANG NDDIVFKAST NPGTAAAAIN KIASGGELSR FMLALKTSLF DKMVKPSIIF DEIDVGIGGE VADKVGERLK KLSSVTQVIV ITHQPQVAGK ADLHIKIEKT QLEKETKVTV KALNLAERQE ELARMISGKT ITKASLKAAK ELLHL

NT Sequence

ATGTTCTACA GTCTCTCAGT TAAAAACTTC ATTCTGATAG ACGAGCTGGA AATTGAGTTT AATAAAGGTT TATGTGTTAT TACCGGTGAA ACGGGAGCCG GTAAATCTAT TTTACTTGAT GCTATTTTAT TTTGCTTAGG TTATAAAACT TCAAATAATA TAATAAAGCG TGGAAAAGAT TACGCTGTTG TTAATATACT ATTCTCTTTA AATGAAGAAA TAAAAAATTT TCTGGTTCAA AATTTGATTG AACCTGAAGA GTTATTGTTT GTAAAGTGTC TGCAAAAAGC CGAAGGACGA AAAAATTTTT TCATTAATAA TCAAGTGGTT ACTAAAGCTC TTATGCAGCA ATTAGCTACT TATTTATTCG AGCTTCATGG ACAAAATAAT AATATTTCTC TTCTAGCAGC AAATACGCAG CGTGATATTT TAGATAGTTA TGGAAATCTT TTGGACTTTC GAGCGGAGCT TTCCAACTGC TATCAGGCTT GGCAAAATAC TCGGAAAGAA ATAGCCGCAA TAACACTTAA ACAAAATTCT ATAGAACAAG AAATTGATTA TTTGAGCTTT GCAACGGAAG AATTAACTAA ACTGAACATT CAAACAGGTG AAGAAGAACA ATTAGCAAAT CTACGAAAAG ATTTGCAAAA TAAAGATAAG GATTTACAGC TAATAAAAGA TATTCTAGAA CAGATTAATA ACCCTGAAAT AAATACATCG ATTAATAGAG CAGAGAAATT ATTAGCAAGG CAAGGCAACA ATGAGCGTTT TGAAGCTATC ATTACAAGTT TAGAGGAAGC CTATAATAAT CTAGAAGAAG CACGCCAAGA ATTATCAAAT CTGTTAGATA GTTTCAATTG TGAGGAATAT AATCTTGAAG AAACAGAAGA AAGATTATTT TTAATAAAAG CTATTAGCCG TAAATATAAT GTTCCTGCTG ATGCGTTAGG TGTATTTTTA GATAAATCTT TAGAGCAGCT CAGTATATTA AAAAACAAAA TAGCAAATAG TAATGAGTTG AAAGCTCAAG AAGTACTACT GCAGAAAAAA TATTATGAAC TAGCGAGTAA TTTATCTGCA AAGCGTCTTA TTGTTGCAAA ACATCTAGAA GAATCGTTGC ATCAGGAGCT AAAGCAGCTT AAAATGGCGA AAGCAATTTT TGAGATTGAG ATAAATGCTA AAGAACCAAC AGCCAACGGT AATGACGATA TTGTGTTTAA AGCGTCTACT AACCCGGGAA CGGCAGCTGC AGCAATTAAT AAAATCGCTT CCGGCGGTGA ATTATCAAGA TTCATGTTAG CTTTAAAAAC TTCTTTGTTT GATAAAATGG TAAAACCATC TATTATATTT GATGAAATAG ATGTCGGGAT TGGCGGAGAG GTCGCAGATA AAGTAGGTGA GCGATTAAAA AAACTTAGTA GCGTTACTCA AGTAATAGTT ATTACGCACC AGCCGCAAGT AGCAGGTAAA GCGGATTTGC ATATAAAAAT CGAGAAAACA CAGCTAGAGA AAGAAACGAA AGTAACGGTA AAAGCTTTAA ATTTAGCTGA AAGACAGGAG GAACTCGCCC GCATGATTTC AGGTAAAACA ATCACCAAAG CAAGTTTAAA AGCAGCAAAG GAATTGTTGC ATTTG

Details for RiafA.17528.a.B1.GE35895

HARVESTED ON:	8/14/2012
SEQUENCED ON:	8/18/2012
EXPECTED MW:	62kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	55

Validated AA Sequence

Validated NT Sequence

ttttntttac tttaagaagg aganatacca tggctcacca ccaccaccac catatgttct acagtctctc agttaaaaac ttcattctga tagacgagct ggaaattgag tttaataaag gtttatgtgt tattaccggt gaaacgggag ccggtaaatc tattttactt gatgctattt tattttgctt aggttataaa acttcaaata atataataaa gcgtggaaaa gattacgctg ttgttaatat actattctct ttaaatgaag aaataaaaaa ttttctggtt caaaatttga ttgaacctga agagttattg tttgtaaagt gtctgcaaaa agccgaagga cgaaaaaatt ttttcattaa taatcaagtg gttactaaag ctcttatgca gcaattagct acttatttat tcgagcttca tggacaaaat aataatattt ctcttctagc agcaaatacg cagcgtgata ttttagatag ttatggaaat cttttggact ttcgagcgga gctttccaaa tgctatcagg cttggcaaaa tactcggaaa gaaatagccg caataacact taagcaaaat tctatagaac aagaaattga ttatttgagc tttgcaacgg aaganttaac taaactgaac attcaaacag gtgaagaaga acaattagca aatctacgaa aagatttgca aaataaagat aaggatttac agctaataaa agatattcta gaacagatta ataaccctga aatnnntaca tcgattaata gagcagagaa attattagca aggcaaggca acaatgagcg ttttgaagct atcattacaa gtttagagga agcctataat aatctagaag aancacgcca agaattatca aatctgttag atagtttcaa ttatgaggaa tataatcttg aagaancaga agannattat ttttaataaa agctattagc cgtaaatata atgntcctgc tgatgcgttn gngnantttt taganaannt ttnnagcagc tcagtnnant naaaaannaa anngcaanna gtangnntnn angctcnnnn nactnctgnn naaaaaannn tnnnantann nnnnnnnnnc tgnnnnnnnn ntnnnnnnng nnannnnnnn nnnnnnnnnn nnncnnnnnn ntnannnnnn nnnnnnnnnn nnnnnna

Expected Protein Sequence

MAHHHHHHMF YSLSVKNFIL IDELEIEFNK GLCVITGETG AGKSILLDAI LFCLGYKTSN NIIKRGKDYA VVNILFSLNE EIKNFLVQNL IEPEELLFVK CLQKAEGRKN FFINNQVVTK ALMQQLATYL FELHGQNNNI SLLAANTQRD ILDSYGNLLD FRAELSNCYQ AWQNTRKEIA AITLKQNSIE QEIDYLSFAT EELTKLNIQT GEEEQLANLR KDLQNKDKDL QLIKDILEQI NNPEINTSIN RAEKLLARQG NNERFEAIIT SLEEAYNNLE EARQELSNLL DSFNCEEYNL EETEERLFLI KAISRKYNVP ADALGVFLDK SLEQLSILKN KIANSNELKA QEVLLQKKYY ELASNLSAKR LIVAKHLEES LHQELKQLKM AKAIFEIEIN AKEPTANGND DIVFKASTNP GTAAAAINKI ASGGELSRFM LALKTSLFDK MVKPSIIFDE IDVGIGGEVA DKVGERLKKL SSVTQVIVIT HQPQVAGKAD LHIKIEKTQL EKETKVTVKA LNLAERQEEL ARMISGKTIT KASLKAAKEL LHL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttct acagtctctc agttaaaaac ttcattctga tagacgagct ggaaattgag tttaataaag gtttatgtgt tattaccggt gaaacgggag ccggtaaatc tattttactt gatgctattt tattttgctt aggttataaa acttcaaata atataataaa gcgtggaaaa gattacgctg ttgttaatat actattctct ttaaatgaag aaataaaaaa ttttctggtt caaaatttga ttgaacctga agagttattg tttgtaaagt gtctgcaaaa agccgaagga cgaaaaaatt ttttcattaa taatcaagtg gttactaaag ctcttatgca gcaattagct acttatttat tcgagcttca tggacaaaat aataatattt ctcttctagc agcaaatacg cagcgtgata ttttagatag ttatggaaat cttttggact ttcgagcgga gctttccaac tgctatcagg cttggcaaaa tactcggaaa gaaatagccg caataacact taaacaaaat tctatagaac aagaaattga ttatttgagc tttgcaacgg aagaattaac taaactgaac attcaaacag gtgaagaaga acaattagca aatctacgaa aagatttgca aaataaagat aaggatttac agctaataaa agatattcta gaacagatta ataaccctga aataaataca tcgattaata gagcagagaa attattagca aggcaaggca acaatgagcg ttttgaagct atcattacaa gtttagagga agcctataat aatctagaag aagcacgcca agaattatca aatctgttag atagtttcaa ttgtgaggaa tataatcttg aagaaacaga agaaagatta tttttaataa aagctattag ccgtaaatat aatgttcctg ctgatgcgtt aggtgtattt ttagataaat ctttagagca gctcagtata ttaaaaaaca aaatagcaaa tagtaatgag ttgaaagctc aagaagtact actgcagaaa aaatattatg aactagcgag taatttatct gcaaagcgtc ttattgttgc aaaacatcta gaagaatcgt tgcatcagga gctaaagcag cttaaaatgg cgaaagcaat ttttgagatt gagataaatg ctaaagaacc aacagccaac ggtaatgacg atattgtgtt taaagcgtct actaacccgg gaacggcagc tgcagcaatt aataaaatcg cttccggcgg tgaattatca agattcatgt tagctttaaa aacttctttg tttgataaaa tggtaaaacc atctattata tttgatgaaa tagatgtcgg gattggcgga gaggtcgcag ataaagtagg tgagcgatta aaaaaactta gtagcgttac tcaagtaata gttattacgc accagccgca agtagcaggt aaagcggatt tgcatataaa aatcgagaaa acacagctag agaaagaaac gaaagtaacg gtaaaagctt taaatttagc tgaaagacag gaggaactcg cccgcatgat ttcaggtaaa acaatcacca aagcaagttt aaaagcagca aaggaattgt tgcatttgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat