SSGCID: Target Page

RiafA.17320.a

Putative ATPase n2B

CENTER ID:	RiafA.17320.a
ORGANISM:	Rickettsia africae ESF-5
ASSOCIATED DISEASE:	African tick-bite fever
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiafA.17320.a.B1.GE35846	Full length( RiafA.17320.a )		1	350

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiafA.17320.a.B1.PS01687	Full length( RiafA.17320.a )		1	350

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|347255.3.peg.312
RefSeq:	YP_002844955.1
UniProt:	C3PMQ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIKSSTSLIR DSKQTALQEE LKQLAAALNK PKSLFKFFNQ NNLESGIYLY GPVGSGKTML MNSFCEELTT PKIIIHYQNF MQEIHKSMHK LQTANQKDII PNIAKNYTKQ TKVLCIDEFE IKDITDAMII GRLFNELIKQ NIFIFITSNT SPNNLYKDGL QRESFLPFIK IINNTFYVKY LDNQHDYRFD KALRVKEARI IYPLTLENQN KLEKIITDIS DNNLAPQNIQ VLGREISFQK VHKRILVTDY NELFTRGLSY IDYVNICQNF NVIIVGNVHT IDSNDTNTAV RFINFIDNAY FYKILLFMSL EDNPNKIYQG SARAAEFKRT ISRLNEMNSE SYLLNNDFKN

NT Sequence

ATGATTAAAT CCTCTACTTC ATTAATACGA GATTCAAAAC AAACTGCTTT ACAAGAAGAA TTAAAACAGC TGGCAGCAGC ACTTAATAAA CCTAAAAGTC TATTCAAATT CTTTAATCAA AACAACTTAG AAAGCGGAAT TTATTTATAC GGTCCGGTGG GCAGCGGTAA AACTATGCTC ATGAATTCTT TTTGTGAAGA ATTAACCACA CCTAAAATAA TAATACATTA TCAGAACTTT ATGCAGGAAA TTCATAAATC TATGCATAAA TTACAAACAG CAAATCAGAA AGATATCATC CCTAACATTG CTAAAAACTA TACTAAACAA ACTAAAGTGT TATGTATAGA TGAGTTTGAA ATTAAAGATA TTACTGATGC AATGATAATC GGTAGATTAT TTAATGAATT AATAAAGCAA AATATTTTTA TTTTTATAAC TTCTAACACG AGTCCCAATA ATCTGTACAA AGACGGATTA CAACGAGAAT CTTTCTTACC TTTTATAAAG ATAATCAATA ATACATTTTA TGTTAAATAT CTAGATAATC AACATGATTA TCGATTTGAT AAAGCGTTAA GAGTTAAAGA GGCAAGGATA ATTTATCCTT TAACTTTAGA AAACCAAAAC AAACTTGAAA AAATTATCAC GGATATTAGT GATAATAATC TTGCTCCTCA AAATATACAG GTTCTAGGTA GAGAAATATC CTTTCAAAAA GTGCATAAAA GAATATTAGT AACAGATTAT AATGAATTAT TTACAAGAGG CTTAAGCTAT ATCGATTATG TTAATATTTG CCAAAATTTT AACGTTATTA TAGTGGGGAA CGTTCATACT ATCGATTCTA ACGATACAAA TACAGCCGTT AGATTTATCA ACTTTATCGA TAATGCCTAT TTCTATAAAA TACTCTTATT TATGAGCCTA GAAGATAACC CAAATAAAAT ATATCAAGGT TCAGCACGAG CCGCAGAATT TAAACGCACT ATTTCAAGAC TAAATGAAAT GAATAGCGAA TCTTATTTAT TAAATAATGA TTTTAAGAAT

Details for RiafA.17320.a.B1.GE35846

HARVESTED ON:	8/14/2012
SEQUENCED ON:	8/18/2012
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	91
PERCENT COVERAGE:	104

Validated AA Sequence

MAHHHHHHMI KSSTSLIRDS KQTALQEELK QLAAALNKPK SLFKFFNQNN LESGIYLYGP VGSGKTMLMN SFCEELTTPK IIIHYQNFMQ EIHKSMHKLQ TANQKDIIPN IAKNYTKQTK VLCIDEFEIK DITDAMIIGR LFNELIKQNI FIFITSNTSP NNLYKDGLQR ESFLPFIKII NNTFYVKYLD NQHDYRFDKA LRVKEARIIY PLTLENQNKL EKIITDISDN NLAPQNIQVL GREISFQKVH KRILVTDYNE LFTRGLSYID YVNICQNFNV IIVGNVHTID SNDTNTAVRF INFIDNAXFY KILLFMSLXX XPNKIXXXSA RAAXXXALFX XXXNXXXXXX XXFXXXXXXX XXXXXXXXXX XXXX

Validated NT Sequence

antttnnnnn ntttaagaag gaganatacc atggctcacc accaccacca ccatatgatt aaatcctcta cttcattaat acgagattca aaacaaactg ctttacaaga agaattaaaa cagctggcag cagcacttaa taaacctaaa agtctattca aattctttaa tcaaaacaac ttagaaagcg gaatttattt atacggtccg gtgggcagcg gtaaaactat gctcatgaat tctttttgtg aagaattaac cacacctaaa ataataatac attatcagaa ctttatgcag gaaattcata aatctatgca taaattacaa acagcaaatc agaaagatat catccctaac attgctaaaa actatactaa acaaactaaa gtgttatgta tagatgagtt tgaaattaaa gatattactg atgcaatgat aatcggtaga ttatttaatg aattaataaa gcaaaatatt tttattttta taacttctaa cacgagtccc aataatctgt acaaagacgg attacaacga gaatctttct taccttttat aaagataatc aataatacat tttatgttaa atatctagat aatcaacatg attatcgatt tgataaagcg ttaagagtta aagaggcaag gataatttat cctttaactt tagaaaacca aaacaaactt gaaaaaatta tcacggatat tagtgataat aatcttgctc ctcaaaatat acaggttcta ggtagagaaa tatcctttca aaaagtgcat aaaagaatat tagtaacaga ttataatgaa ttatttacaa gaggcttaag ctatatcgat tatgttaata tttgccaaaa ttttaacgtt attatagtgg ggaacgttca tactatcgat tctaacgata caaatacagc cgttagattt atcaacttta tcgataatgc ctanttctat aaaatactct tatttatgag cctanaagan nacccnaata aaatatnnca nngntcagca cgagcngcan antnnancgc nctatttcan nctnannnna atganannna nnnnntnntn annngntttn nnnnnnnnnt annnnnnnnn nnnnnnnnnn nncnnnnnnn nnnnnnnnnn nntnnnnnnn nnt

Expected Protein Sequence

MAHHHHHHMI KSSTSLIRDS KQTALQEELK QLAAALNKPK SLFKFFNQNN LESGIYLYGP VGSGKTMLMN SFCEELTTPK IIIHYQNFMQ EIHKSMHKLQ TANQKDIIPN IAKNYTKQTK VLCIDEFEIK DITDAMIIGR LFNELIKQNI FIFITSNTSP NNLYKDGLQR ESFLPFIKII NNTFYVKYLD NQHDYRFDKA LRVKEARIIY PLTLENQNKL EKIITDISDN NLAPQNIQVL GREISFQKVH KRILVTDYNE LFTRGLSYID YVNICQNFNV IIVGNVHTID SNDTNTAVRF INFIDNAYFY KILLFMSLED NPNKIYQGSA RAAEFKRTIS RLNEMNSESY LLNNDFKN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatta aatcctctac ttcattaata cgagattcaa aacaaactgc tttacaagaa gaattaaaac agctggcagc agcacttaat aaacctaaaa gtctattcaa attctttaat caaaacaact tagaaagcgg aatttattta tacggtccgg tgggcagcgg taaaactatg ctcatgaatt ctttttgtga agaattaacc acacctaaaa taataataca ttatcagaac tttatgcagg aaattcataa atctatgcat aaattacaaa cagcaaatca gaaagatatc atccctaaca ttgctaaaaa ctatactaaa caaactaaag tgttatgtat agatgagttt gaaattaaag atattactga tgcaatgata atcggtagat tatttaatga attaataaag caaaatattt ttatttttat aacttctaac acgagtccca ataatctgta caaagacgga ttacaacgag aatctttctt accttttata aagataatca ataatacatt ttatgttaaa tatctagata atcaacatga ttatcgattt gataaagcgt taagagttaa agaggcaagg ataatttatc ctttaacttt agaaaaccaa aacaaacttg aaaaaattat cacggatatt agtgataata atcttgctcc tcaaaatata caggttctag gtagagaaat atcctttcaa aaagtgcata aaagaatatt agtaacagat tataatgaat tatttacaag aggcttaagc tatatcgatt atgttaatat ttgccaaaat tttaacgtta ttatagtggg gaacgttcat actatcgatt ctaacgatac aaatacagcc gttagattta tcaactttat cgataatgcc tatttctata aaatactctt atttatgagc ctagaagata acccaaataa aatatatcaa ggttcagcac gagccgcaga atttaaacgc actatttcaa gactaaatga aatgaatagc gaatcttatt tattaaataa tgattttaag aattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for RiafA.17320.a.B1.PS01687

PURIFICATION DATe:	11/7/2012
CONCENTRATION:	39.7mg/ml
OBSERVED MW:	40kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	110µl
PERCENT IDENTITY:	91
PERCENT COVERAGE:	104

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMI KSSTSLIRDS KQTALQEELK QLAAALNKPK SLFKFFNQNN LESGIYLYGP VGSGKTMLMN SFCEELTTPK IIIHYQNFMQ EIHKSMHKLQ TANQKDIIPN IAKNYTKQTK VLCIDEFEIK DITDAMIIGR LFNELIKQNI FIFITSNTSP NNLYKDGLQR ESFLPFIKII NNTFYVKYLD NQHDYRFDKA LRVKEARIIY PLTLENQNKL EKIITDISDN NLAPQNIQVL GREISFQKVH KRILVTDYNE LFTRGLSYID YVNICQNFNV IIVGNVHTID SNDTNTAVRF INFIDNAYFY KILLFMSLED NPNKIYQGSA RAAEFKRTIS RLNEMNSESY LLNNDFKN

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATTA AATCCTCTAC TTCATTAATA CGAGATTCAA AACAAACTGC TTTACAAGAA GAATTAAAAC AGCTGGCAGC AGCACTTAAT AAACCTAAAA GTCTATTCAA ATTCTTTAAT CAAAACAACT TAGAAAGCGG AATTTATTTA TACGGTCCGG TGGGCAGCGG TAAAACTATG CTCATGAATT CTTTTTGTGA AGAATTAACC ACACCTAAAA TAATAATACA TTATCAGAAC TTTATGCAGG AAATTCATAA ATCTATGCAT AAATTACAAA CAGCAAATCA GAAAGATATC ATCCCTAACA TTGCTAAAAA CTATACTAAA CAAACTAAAG TGTTATGTAT AGATGAGTTT GAAATTAAAG ATATTACTGA TGCAATGATA ATCGGTAGAT TATTTAATGA ATTAATAAAG CAAAATATTT TTATTTTTAT AACTTCTAAC ACGAGTCCCA ATAATCTGTA CAAAGACGGA TTACAACGAG AATCTTTCTT ACCTTTTATA AAGATAATCA ATAATACATT TTATGTTAAA TATCTAGATA ATCAACATGA TTATCGATTT GATAAAGCGT TAAGAGTTAA AGAGGCAAGG ATAATTTATC CTTTAACTTT AGAAAACCAA AACAAACTTG AAAAAATTAT CACGGATATT AGTGATAATA ATCTTGCTCC TCAAAATATA CAGGTTCTAG GTAGAGAAAT ATCCTTTCAA AAAGTGCATA AAAGAATATT AGTAACAGAT TATAATGAAT TATTTACAAG AGGCTTAAGC TATATCGATT ATGTTAATAT TTGCCAAAAT TTTAACGTTA TTATAGTGGG GAACGTTCAT ACTATCGATT CTAACGATAC AAATACAGCC GTTAGATTTA TCAACTTTAT CGATAATGCC TATTTCTATA AAATACTCTT ATTTATGAGC CTAGAAGATA ACCCAAATAA AATATATCAA GGTTCAGCAC GAGCCGCAGA ATTTAAACGC ACTATTTCAA GACTAAATGA AATGAATAGC GAATCTTATT TATTAAATAA TGATTTTAAG AATTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT