RiafA.17300.a

Type I secretion outer membrane protein TolC

CENTER ID: RiafA.17300.a
ORGANISM: Rickettsia africae ESF-5
ASSOCIATED DISEASE: African tick-bite fever
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiafA.17300.a.B2.GE35554 Mature protein( RiafA.17300.a ) 19 453
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiafA.17300.a.B2.PD00339 Mature protein( RiafA.17300.a ) 19 453
RiafA.17300.a.B2.PD00340 Mature protein( RiafA.17300.a ) 19 453

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|347255.3.peg.341
RefSeq: YP_002844973.1
UniProt: C3PMS0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRKLITFIVT LLLTSSVAAV DLQGALTEGY KNNEELKAAR IKFLDSIEQF PRAFSGFMPS AGLQINRQHN KTKYNKKYAD RLGLTPRDTD SDQGALTIQQ SLFNGGSSVA ALKAAQAGFR AARGAYYAGE QKILLNLITA YLDCFESKEK YDISESRVRT NIQQVNTVEE KLRLGEATEV DIATARAGLA AAETNKLAAY ADFQGKKANF IRVFGIEPTN ITMPELPKML PASLDELTGR AAKLNPDIDS ARHNVTAAKA LEMAEKGKLL PQVSVQLQSG RTYYNPQGDI NAINNRSYTT TLSVNIPIYP EGGAQYSRIR SAKNQTRNSA VQLDNVIKQT QAGVVSVWEG FEAAKSRIVA ANQGVDAAQI SYDGTVQEEI VGSKTILDVL TAAEKLYEAK ITRVDAYKSS VLAAYQMKLL TGELTAQSLK LKVKYFSPEE EFKTIKKKMF IGF
NT Sequence
ATGCGTAAAT TAATTACATT TATTGTTACA TTATTACTAA CTAGTTCGGT TGCAGCAGTT GATTTGCAGG GAGCTTTAAC TGAAGGATAT AAGAATAATG AGGAGCTGAA AGCTGCTAGA ATTAAGTTTT TAGACTCGAT TGAGCAGTTT CCGCGAGCTT TTTCGGGGTT TATGCCTAGT GCCGGATTAC AGATTAATAG ACAACATAAT AAGACTAAAT ATAATAAAAA ATATGCTGAT AGGCTTGGTC TTACTCCAAG GGACACAGAT AGTGATCAAG GTGCATTAAC AATTCAACAG TCTCTATTTA ATGGTGGTTC TAGTGTTGCG GCTCTTAAAG CTGCTCAAGC AGGATTTAGA GCTGCACGGG GTGCATATTA TGCCGGTGAA CAAAAAATAT TATTAAATTT AATAACTGCG TATCTTGATT GTTTTGAGAG CAAAGAGAAA TATGATATTT CCGAAAGTAG AGTTCGCACT AACATACAAC AAGTTAACAC CGTTGAGGAA AAATTAAGAC TTGGAGAAGC AACGGAAGTA GATATAGCAA CCGCAAGAGC GGGGCTTGCA GCAGCAGAAA CGAATAAACT AGCTGCTTAT GCCGATTTTC AAGGAAAAAA AGCAAATTTT ATTAGAGTAT TTGGAATAGA GCCGACTAAT ATAACTATGC CTGAGTTACC TAAAATGTTA CCTGCTTCAT TAGATGAGTT AACTGGAAGA GCTGCTAAAC TCAATCCTGA TATTGATTCG GCAAGGCATA ATGTAACTGC GGCTAAAGCT TTAGAAATGG CAGAAAAAGG GAAATTATTA CCGCAAGTAA GTGTGCAACT GCAATCCGGT AGAACATACT ACAATCCACA AGGTGATATA AATGCTATAA ATAATAGAAG CTATACTACT ACTCTTTCGG TAAATATCCC TATTTATCCT GAGGGAGGAG CTCAATATTC AAGAATCAGA TCGGCTAAAA ATCAAACAAG AAATAGTGCA GTTCAACTTG ATAATGTGAT AAAGCAGACC CAAGCAGGGG TTGTAAGCGT GTGGGAGGGA TTTGAAGCAG CAAAATCTCG TATTGTTGCC GCTAATCAAG GAGTGGATGC TGCTCAAATA TCATATGACG GTACTGTGCA AGAGGAAATA GTCGGATCTA AAACAATATT AGATGTTTTA ACTGCCGCAG AAAAATTATA CGAAGCAAAA ATAACTCGTG TGGATGCGTA TAAATCTTCA GTACTTGCGG CATATCAAAT GAAATTGTTA ACTGGCGAGC TAACGGCTCA AAGTTTAAAA CTTAAAGTAA AATATTTTAG TCCTGAAGAA GAGTTTAAGA CTATTAAAAA GAAAATGTTT ATAGGTTTC
Details for RiafA.17300.a.B2.GE35554
HARVESTED ON: 6/12/2012
SEQUENCED ON: 6/12/2012
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 86
Validated AA Sequence
MPSAGLQINR QHNKTKYNKK YADRLGLTPR DTDSDQGALT IQQSLFNGGS SVAALKAAQA GFRAARGAYY AGEQKILLNL ITAYLDCFES KEKYDISESR VRTNIQQVNT VEEKLRLGEA TEVDIATARA GLAAAETNKL AAYADFQGKK ANFIRVFGIE PTNITMPELP KMLPASLDEL TGRAAKLNPD IDAARHNVTA AKALEMAEKG KLLPQVSVQL QSGRTYYNPQ GDINAINNRS YTTTLSVNIP IYPEGGAQYS RIRSAKNQTR NSAVQLDNVI KQTQAGVVSV WEGFEAAKSR IVAANQGVDA AQISYDGTVQ EEIVGSKTIL DVLTAEEKLY EAKITRVDAY KSSVLAAYQM KLLTGELTAQ SLKLKVKYFS PEEE
Validated NT Sequence
aaactcttct tcaggactaa aatattttac tttaagtttt aaactttgag ccgttagctc gccagttaac aatttcattt gatatgccgc aagtactgaa gatttatacg catccacacg agttattttt gcttcgtata atttttcttc ggcagttaaa acatctaata ttgttttaga tccgactatt tcctcttgca cagtaccgtc atatgatatt tgagcagcat ccactccttg attagcggca acaatacgag attttgctgc ttcaaatccc tcccacacgc ttacaacccc tgcttgggtc tgctttatca cattatcaag ttgaactgca ctatttcttg tttgattttt agccgatctg attcttgaat attgagctcc tccctcagga taaataggga tatttaccga aagagtagta gtatagcttc tattatttat agcatttata tcaccttgtg gattgtagta tgttctaccg gattgcagtt gcacacttac ttgcggtaat aatttccctt tttctgccat ttctaaagct ttagccgcag ttacattatg ccttgccgca tcaatatcag gattgagttt agcagctctt ccagttaact catctaatga agcaggtaac attttaggta actcaggcat agttatatta gtcggctcta ttccaaatac tctaataaaa tttgcttttt ttccttgaaa atcggcataa gcagctagtt tattcgtttc tgctgctgca agccccgctc ttgcggttgc tatatctact tccgttgctt ctccaagtct taatttttcc tcaacggtgt taacttgttg tatgttagtg cgaactctac tttcggaaat atcatatttc tctttgctct caaaacaatc aagatacgca gttattaaat ttaataatat tttttgttca ccggcataat atgcaccccg tgcagctcta aatcctgctt gagcagcttt aagagccgca acactagaac caccattaaa tagagactgt tgaattgtta atgcaccttg atcactatct gtgtcccttg gagtaagacc aagcctatca gcatattttt tattatattt agtcttatta tgttgtctat taatctgtaa tccggcacta ggcataaacc ccgaaaaagc tcgcggaaac tgctcaatcg agtctaaaaa cttaattcta gcagctttca gctcctcatt attcttatat ccttcagtta aagctccctg caaatcaact gcca
Expected Protein Sequence
MAHHHHHHMA VDLQGALTEG YKNNEELKAA RIKFLDSIEQ FPRAFSGFMP SAGLQINRQH NKTKYNKKYA DRLGLTPRDT DSDQGALTIQ QSLFNGGSSV AALKAAQAGF RAARGAYYAG EQKILLNLIT AYLDCFESKE KYDISESRVR TNIQQVNTVE EKLRLGEATE VDIATARAGL AAAETNKLAA YADFQGKKAN FIRVFGIEPT NITMPELPKM LPASLDELTG RAAKLNPDID SARHNVTAAK ALEMAEKGKL LPQVSVQLQS GRTYYNPQGD INAINNRSYT TTLSVNIPIY PEGGAQYSRI RSAKNQTRNS AVQLDNVIKQ TQAGVVSVWE GFEAAKSRIV AANQGVDAAQ ISYDGTVQEE IVGSKTILDV LTAAEKLYEA KITRVDAYKS SVLAAYQMKL LTGELTAQSL KLKVKYFSPE EEFKTIKKKM FIGF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag ttgatttgca gggagcttta actgaaggat ataagaataa tgaggagctg aaagctgcta gaattaagtt tttagactcg attgagcagt ttccgcgagc tttttcgggg tttatgccta gtgccggatt acagattaat agacaacata ataagactaa atataataaa aaatatgctg ataggcttgg tcttactcca agggacacag atagtgatca aggtgcatta acaattcaac agtctctatt taatggtggt tctagtgttg cggctcttaa agctgctcaa gcaggattta gagctgcacg gggtgcatat tatgccggtg aacaaaaaat attattaaat ttaataactg cgtatcttga ttgttttgag agcaaagaga aatatgatat ttccgaaagt agagttcgca ctaacataca acaagttaac accgttgagg aaaaattaag acttggagaa gcaacggaag tagatatagc aaccgcaaga gcggggcttg cagcagcaga aacgaataaa ctagctgctt atgccgattt tcaaggaaaa aaagcaaatt ttattagagt atttggaata gagccgacta atataactat gcctgagtta cctaaaatgt tacctgcttc attagatgag ttaactggaa gagctgctaa actcaatcct gatattgatt cggcaaggca taatgtaact gcggctaaag ctttagaaat ggcagaaaaa gggaaattat taccgcaagt aagtgtgcaa ctgcaatccg gtagaacata ctacaatcca caaggtgata taaatgctat aaataataga agctatacta ctactctttc ggtaaatatc cctatttatc ctgagggagg agctcaatat tcaagaatca gatcggctaa aaatcaaaca agaaatagtg cagttcaact tgataatgtg ataaagcaga cccaagcagg ggttgtaagc gtgtgggagg gatttgaagc agcaaaatct cgtattgttg ccgctaatca aggagtggat gctgctcaaa tatcatatga cggtactgtg caagaggaaa tagtcggatc taaaacaata ttagatgttt taactgccgc agaaaaatta tacgaagcaa aaataactcg tgtggatgcg tataaatctt cagtacttgc ggcatatcaa atgaaattgt taactggcga gctaacggct caaagtttaa aacttaaagt aaaatatttt agtcctgaag aagagtttaa gactattaaa aagaaaatgt ttataggttt ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for RiafA.17300.a.B2.PD00339
PURIFICATION DATe: 9/10/2014
CONCENTRATION: 11.1mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 17
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 86
Protocol Notes
notes unavailable
Validated AA Sequence
MPSAGLQINR QHNKTKYNKK YADRLGLTPR DTDSDQGALT IQQSLFNGGS SVAALKAAQA GFRAARGAYY AGEQKILLNL ITAYLDCFES KEKYDISESR VRTNIQQVNT VEEKLRLGEA TEVDIATARA GLAAAETNKL AAYADFQGKK ANFIRVFGIE PTNITMPELP KMLPASLDEL TGRAAKLNPD IDAARHNVTA AKALEMAEKG KLLPQVSVQL QSGRTYYNPQ GDINAINNRS YTTTLSVNIP IYPEGGAQYS RIRSAKNQTR NSAVQLDNVI KQTQAGVVSV WEGFEAAKSR IVAANQGVDA AQISYDGTVQ EEIVGSKTIL DVLTAEEKLY EAKITRVDAY KSSVLAAYQM KLLTGELTAQ SLKLKVKYFS PEEE
Validated NT Sequence
aaactcttct tcaggactaa aatattttac tttaagtttt aaactttgag ccgttagctc gccagttaac aatttcattt gatatgccgc aagtactgaa gatttatacg catccacacg agttattttt gcttcgtata atttttcttc ggcagttaaa acatctaata ttgttttaga tccgactatt tcctcttgca cagtaccgtc atatgatatt tgagcagcat ccactccttg attagcggca acaatacgag attttgctgc ttcaaatccc tcccacacgc ttacaacccc tgcttgggtc tgctttatca cattatcaag ttgaactgca ctatttcttg tttgattttt agccgatctg attcttgaat attgagctcc tccctcagga taaataggga tatttaccga aagagtagta gtatagcttc tattatttat agcatttata tcaccttgtg gattgtagta tgttctaccg gattgcagtt gcacacttac ttgcggtaat aatttccctt tttctgccat ttctaaagct ttagccgcag ttacattatg ccttgccgca tcaatatcag gattgagttt agcagctctt ccagttaact catctaatga agcaggtaac attttaggta actcaggcat agttatatta gtcggctcta ttccaaatac tctaataaaa tttgcttttt ttccttgaaa atcggcataa gcagctagtt tattcgtttc tgctgctgca agccccgctc ttgcggttgc tatatctact tccgttgctt ctccaagtct taatttttcc tcaacggtgt taacttgttg tatgttagtg cgaactctac tttcggaaat atcatatttc tctttgctct caaaacaatc aagatacgca gttattaaat ttaataatat tttttgttca ccggcataat atgcaccccg tgcagctcta aatcctgctt gagcagcttt aagagccgca acactagaac caccattaaa tagagactgt tgaattgtta atgcaccttg atcactatct gtgtcccttg gagtaagacc aagcctatca gcatattttt tattatattt agtcttatta tgttgtctat taatctgtaa tccggcacta ggcataaacc ccgaaaaagc tcgcggaaac tgctcaatcg agtctaaaaa cttaattcta gcagctttca gctcctcatt attcttatat ccttcagtta aagctccctg caaatcaact gcca
Expressed Protein Sequence
MAHHHHHHMA VDLQGALTEG YKNNEELKAA RIKFLDSIEQ FPRAFSGFMP SAGLQINRQH NKTKYNKKYA DRLGLTPRDT DSDQGALTIQ QSLFNGGSSV AALKAAQAGF RAARGAYYAG EQKILLNLIT AYLDCFESKE KYDISESRVR TNIQQVNTVE EKLRLGEATE VDIATARAGL AAAETNKLAA YADFQGKKAN FIRVFGIEPT NITMPELPKM LPASLDELTG RAAKLNPDID SARHNVTAAK ALEMAEKGKL LPQVSVQLQS GRTYYNPQGD INAINNRSYT TTLSVNIPIY PEGGAQYSRI RSAKNQTRNS AVQLDNVIKQ TQAGVVSVWE GFEAAKSRIV AANQGVDAAQ ISYDGTVQEE IVGSKTILDV LTAAEKLYEA KITRVDAYKS SVLAAYQMKL LTGELTAQSL KLKVKYFSPE EEFKTIKKKM FIGF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAG TTGATTTGCA GGGAGCTTTA ACTGAAGGAT ATAAGAATAA TGAGGAGCTG AAAGCTGCTA GAATTAAGTT TTTAGACTCG ATTGAGCAGT TTCCGCGAGC TTTTTCGGGG TTTATGCCTA GTGCCGGATT ACAGATTAAT AGACAACATA ATAAGACTAA ATATAATAAA AAATATGCTG ATAGGCTTGG TCTTACTCCA AGGGACACAG ATAGTGATCA AGGTGCATTA ACAATTCAAC AGTCTCTATT TAATGGTGGT TCTAGTGTTG CGGCTCTTAA AGCTGCTCAA GCAGGATTTA GAGCTGCACG GGGTGCATAT TATGCCGGTG AACAAAAAAT ATTATTAAAT TTAATAACTG CGTATCTTGA TTGTTTTGAG AGCAAAGAGA AATATGATAT TTCCGAAAGT AGAGTTCGCA CTAACATACA ACAAGTTAAC ACCGTTGAGG AAAAATTAAG ACTTGGAGAA GCAACGGAAG TAGATATAGC AACCGCAAGA GCGGGGCTTG CAGCAGCAGA AACGAATAAA CTAGCTGCTT ATGCCGATTT TCAAGGAAAA AAAGCAAATT TTATTAGAGT ATTTGGAATA GAGCCGACTA ATATAACTAT GCCTGAGTTA CCTAAAATGT TACCTGCTTC ATTAGATGAG TTAACTGGAA GAGCTGCTAA ACTCAATCCT GATATTGATT CGGCAAGGCA TAATGTAACT GCGGCTAAAG CTTTAGAAAT GGCAGAAAAA GGGAAATTAT TACCGCAAGT AAGTGTGCAA CTGCAATCCG GTAGAACATA CTACAATCCA CAAGGTGATA TAAATGCTAT AAATAATAGA AGCTATACTA CTACTCTTTC GGTAAATATC CCTATTTATC CTGAGGGAGG AGCTCAATAT TCAAGAATCA GATCGGCTAA AAATCAAACA AGAAATAGTG CAGTTCAACT TGATAATGTG ATAAAGCAGA CCCAAGCAGG GGTTGTAAGC GTGTGGGAGG GATTTGAAGC AGCAAAATCT CGTATTGTTG CCGCTAATCA AGGAGTGGAT GCTGCTCAAA TATCATATGA CGGTACTGTG CAAGAGGAAA TAGTCGGATC TAAAACAATA TTAGATGTTT TAACTGCCGC AGAAAAATTA TACGAAGCAA AAATAACTCG TGTGGATGCG TATAAATCTT CAGTACTTGC GGCATATCAA ATGAAATTGT TAACTGGCGA GCTAACGGCT CAAAGTTTAA AACTTAAAGT AAAATATTTT AGTCCTGAAG AAGAGTTTAA GACTATTAAA AAGAAAATGT TTATAGGTTT CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT
Details for RiafA.17300.a.B2.PD00340
PURIFICATION DATe: 9/10/2014
CONCENTRATION: 2mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20mM Tris-HCl, pH 8.0, 200mM NaCl, 1% glycerol, 1mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 86
Protocol Notes
notes unavailable
Validated AA Sequence
MPSAGLQINR QHNKTKYNKK YADRLGLTPR DTDSDQGALT IQQSLFNGGS SVAALKAAQA GFRAARGAYY AGEQKILLNL ITAYLDCFES KEKYDISESR VRTNIQQVNT VEEKLRLGEA TEVDIATARA GLAAAETNKL AAYADFQGKK ANFIRVFGIE PTNITMPELP KMLPASLDEL TGRAAKLNPD IDAARHNVTA AKALEMAEKG KLLPQVSVQL QSGRTYYNPQ GDINAINNRS YTTTLSVNIP IYPEGGAQYS RIRSAKNQTR NSAVQLDNVI KQTQAGVVSV WEGFEAAKSR IVAANQGVDA AQISYDGTVQ EEIVGSKTIL DVLTAEEKLY EAKITRVDAY KSSVLAAYQM KLLTGELTAQ SLKLKVKYFS PEEE
Validated NT Sequence
aaactcttct tcaggactaa aatattttac tttaagtttt aaactttgag ccgttagctc gccagttaac aatttcattt gatatgccgc aagtactgaa gatttatacg catccacacg agttattttt gcttcgtata atttttcttc ggcagttaaa acatctaata ttgttttaga tccgactatt tcctcttgca cagtaccgtc atatgatatt tgagcagcat ccactccttg attagcggca acaatacgag attttgctgc ttcaaatccc tcccacacgc ttacaacccc tgcttgggtc tgctttatca cattatcaag ttgaactgca ctatttcttg tttgattttt agccgatctg attcttgaat attgagctcc tccctcagga taaataggga tatttaccga aagagtagta gtatagcttc tattatttat agcatttata tcaccttgtg gattgtagta tgttctaccg gattgcagtt gcacacttac ttgcggtaat aatttccctt tttctgccat ttctaaagct ttagccgcag ttacattatg ccttgccgca tcaatatcag gattgagttt agcagctctt ccagttaact catctaatga agcaggtaac attttaggta actcaggcat agttatatta gtcggctcta ttccaaatac tctaataaaa tttgcttttt ttccttgaaa atcggcataa gcagctagtt tattcgtttc tgctgctgca agccccgctc ttgcggttgc tatatctact tccgttgctt ctccaagtct taatttttcc tcaacggtgt taacttgttg tatgttagtg cgaactctac tttcggaaat atcatatttc tctttgctct caaaacaatc aagatacgca gttattaaat ttaataatat tttttgttca ccggcataat atgcaccccg tgcagctcta aatcctgctt gagcagcttt aagagccgca acactagaac caccattaaa tagagactgt tgaattgtta atgcaccttg atcactatct gtgtcccttg gagtaagacc aagcctatca gcatattttt tattatattt agtcttatta tgttgtctat taatctgtaa tccggcacta ggcataaacc ccgaaaaagc tcgcggaaac tgctcaatcg agtctaaaaa cttaattcta gcagctttca gctcctcatt attcttatat ccttcagtta aagctccctg caaatcaact gcca
Expressed Protein Sequence
MAHHHHHHMA VDLQGALTEG YKNNEELKAA RIKFLDSIEQ FPRAFSGFMP SAGLQINRQH NKTKYNKKYA DRLGLTPRDT DSDQGALTIQ QSLFNGGSSV AALKAAQAGF RAARGAYYAG EQKILLNLIT AYLDCFESKE KYDISESRVR TNIQQVNTVE EKLRLGEATE VDIATARAGL AAAETNKLAA YADFQGKKAN FIRVFGIEPT NITMPELPKM LPASLDELTG RAAKLNPDID SARHNVTAAK ALEMAEKGKL LPQVSVQLQS GRTYYNPQGD INAINNRSYT TTLSVNIPIY PEGGAQYSRI RSAKNQTRNS AVQLDNVIKQ TQAGVVSVWE GFEAAKSRIV AANQGVDAAQ ISYDGTVQEE IVGSKTILDV LTAAEKLYEA KITRVDAYKS SVLAAYQMKL LTGELTAQSL KLKVKYFSPE EEFKTIKKKM FIGF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAG TTGATTTGCA GGGAGCTTTA ACTGAAGGAT ATAAGAATAA TGAGGAGCTG AAAGCTGCTA GAATTAAGTT TTTAGACTCG ATTGAGCAGT TTCCGCGAGC TTTTTCGGGG TTTATGCCTA GTGCCGGATT ACAGATTAAT AGACAACATA ATAAGACTAA ATATAATAAA AAATATGCTG ATAGGCTTGG TCTTACTCCA AGGGACACAG ATAGTGATCA AGGTGCATTA ACAATTCAAC AGTCTCTATT TAATGGTGGT TCTAGTGTTG CGGCTCTTAA AGCTGCTCAA GCAGGATTTA GAGCTGCACG GGGTGCATAT TATGCCGGTG AACAAAAAAT ATTATTAAAT TTAATAACTG CGTATCTTGA TTGTTTTGAG AGCAAAGAGA AATATGATAT TTCCGAAAGT AGAGTTCGCA CTAACATACA ACAAGTTAAC ACCGTTGAGG AAAAATTAAG ACTTGGAGAA GCAACGGAAG TAGATATAGC AACCGCAAGA GCGGGGCTTG CAGCAGCAGA AACGAATAAA CTAGCTGCTT ATGCCGATTT TCAAGGAAAA AAAGCAAATT TTATTAGAGT ATTTGGAATA GAGCCGACTA ATATAACTAT GCCTGAGTTA CCTAAAATGT TACCTGCTTC ATTAGATGAG TTAACTGGAA GAGCTGCTAA ACTCAATCCT GATATTGATT CGGCAAGGCA TAATGTAACT GCGGCTAAAG CTTTAGAAAT GGCAGAAAAA GGGAAATTAT TACCGCAAGT AAGTGTGCAA CTGCAATCCG GTAGAACATA CTACAATCCA CAAGGTGATA TAAATGCTAT AAATAATAGA AGCTATACTA CTACTCTTTC GGTAAATATC CCTATTTATC CTGAGGGAGG AGCTCAATAT TCAAGAATCA GATCGGCTAA AAATCAAACA AGAAATAGTG CAGTTCAACT TGATAATGTG ATAAAGCAGA CCCAAGCAGG GGTTGTAAGC GTGTGGGAGG GATTTGAAGC AGCAAAATCT CGTATTGTTG CCGCTAATCA AGGAGTGGAT GCTGCTCAAA TATCATATGA CGGTACTGTG CAAGAGGAAA TAGTCGGATC TAAAACAATA TTAGATGTTT TAACTGCCGC AGAAAAATTA TACGAAGCAA AAATAACTCG TGTGGATGCG TATAAATCTT CAGTACTTGC GGCATATCAA ATGAAATTGT TAACTGGCGA GCTAACGGCT CAAAGTTTAA AACTTAAAGT AAAATATTTT AGTCCTGAAG AAGAGTTTAA GACTATTAAA AAGAAAATGT TTATAGGTTT CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT