RiafA.17295.a
Glycosyltransferase
| CENTER ID: | RiafA.17295.a |
| ORGANISM: | Rickettsia africae ESF-5 |
| ASSOCIATED DISEASE: | African tick-bite fever |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB/C |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RiafA.17295.a.B1.GE35844 | Full length( RiafA.17295.a ) | 1 | 390 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| RiafA.17295.a.B1.PW36512 | Full length( RiafA.17295.a ) | 1 | 390 |
Structures
7MI0
DEPOSITED: 4/16/2021
DETERMINATION: XRay
CLONE: RiafA.17295.a.B1.GE35844
PROTEIN: RiafA.17295.a.B1.PW36512
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_139219 |
| PATRIC ID: | fig|347255.3.peg.522 |
| RefSeq: | YP_002845109.1 |
| UniProt: | C3PN56 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPSLKSSKRY NKYTILQVVP ALVSGGVERG TIEVAKYLKI LGHTPIIISV GGTLVKELDK EDILHIEMNS NSKNPFVILN NAKLIAEIIK KYNVDIVHTR SRAPAWSSYL ATKWTNAKFL TTFHGVYNIP NSFKKYYNSI MLKGKKVVAV SNFVKQHLLE NYKIDEDKIV VIERGVNCDY FDPANLTPEK LEKCCEKYDV PSNVPIILMP SRMTSWKGHL VLVEALSKLK HRDFYCLMVG DISRHPNFTN RVKELIANLK LQNKIQIFGN DSDIINLYGI SDIIISASIE PEAFGRTIIE GQAMKKLVIA TNIGGAVETI NNNITGFHVE PNNAEALAQK IDYCFSILGT DLAKKIQEAA RHTVINNFSL NLMLRKNLEI YKEILKNSHN
NT Sequence
ATGCCTTCAT TAAAGTCAAG TAAACGATAC AATAAATATA CTATTTTGCA AGTAGTGCCT GCTCTAGTTT CGGGAGGGGT TGAAAGAGGT ACTATAGAAG TTGCAAAATA TCTTAAAATT CTCGGTCATA CTCCTATCAT AATTTCAGTA GGCGGTACTC TAGTTAAAGA GTTAGATAAG GAAGATATAT TGCATATTGA AATGAATAGT AACAGTAAAA ATCCTTTTGT AATTCTGAAT AATGCAAAAT TGATAGCAGA AATAATCAAA AAATATAATG TTGATATAGT TCATACAAGA TCAAGAGCAC CGGCATGGAG TTCATATCTA GCAACAAAAT GGACAAATGC TAAATTTTTG ACTACCTTTC ATGGGGTTTA TAATATCCCG AATAGTTTTA AAAAATATTA TAATAGCATA ATGCTAAAAG GCAAGAAAGT TGTTGCCGTG TCTAATTTTG TAAAACAACA CTTGCTCGAA AACTATAAAA TTGATGAAGA TAAAATAGTA GTAATTGAGC GTGGAGTGAA TTGTGATTAT TTCGATCCCG CAAATTTAAC GCCGGAAAAA CTTGAAAAAT GTTGTGAGAA ATATGATGTA CCGAGTAACG TGCCGATAAT ATTAATGCCT TCTAGAATGA CAAGTTGGAA AGGACATCTT GTTCTAGTAG AAGCATTAAG TAAATTAAAA CATAGAGACT TTTATTGTTT AATGGTCGGT GATATATCTA GACATCCCAA TTTTACTAAT AGAGTAAAAG AGCTTATAGC TAATTTAAAA CTTCAAAATA AAATTCAAAT TTTTGGTAAT GATTCGGATA TAATAAACCT ATATGGGATT TCCGATATTA TCATTTCTGC CTCAATCGAG CCTGAAGCTT TCGGGCGTAC TATTATAGAA GGGCAGGCGA TGAAGAAGCT TGTTATTGCA ACTAATATCG GTGGGGCAGT AGAGACAATA AATAATAATA TAACCGGTTT CCATGTAGAA CCAAATAATG CCGAAGCGTT AGCACAGAAA ATCGATTATT GTTTCTCGAT TTTAGGTACT GATCTTGCTA AGAAAATCCA AGAAGCGGCA AGGCATACGG TAATTAATAA TTTCTCTCTT AATCTGATGC TAAGAAAAAA CCTTGAAATC TATAAAGAAA TCTTAAAAAA CTCCCATAAT
Details for RiafA.17295.a.B1.GE35844
| HARVESTED ON: | 8/14/2012 |
| SEQUENCED ON: | 8/18/2012 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS SLKSSKRYNK YTILQVVPAL VSGGVERGTI EVAKYLKILG HTPIIISVGG TLVKELDKED ILHIEMNSNS KNPFVILNNA KLIAEIIKKY NVDIVHTRSR APAWSSYLAT KWTNAKFLTT FHGVYNIPNS FKKYYNSIML KGKKVVAVSN FVKQHLLENY KIDEDKIVVI ERGVNCDYFD PANLTPEKLE KCCEKYDVPS NVPIILMPSR MTSWKGHLVL VEALSKLKHR DFYCLMVGDI SRHPNFTNRV KELIANLKLQ NKIQIFGNDS DIINLYGISD IIISASIEPE AFGRTIIEGQ AMKKLVIATN IGGAVETINN NITGFHVEPN NAEALAQKID YCFSILGTDL AKKIQEAARH TVINNFSLNL MLRKNLEIYK EILKNSHN
Validated NT Sequence
atggctcacc accaccacca ccatatgtct tcattaaagt caagtaaacg atacaataaa tatactattt tgcaagtagt gcctgctcta gtttcgggag gggttgaaag aggtactata gaagttgcaa aatatcttaa aattctcggt catactccta tcataatttc agtaggcggt actctagtta aagagttaga taaggaagat atattgcata ttgaaatgaa tagtaacagt aaaaatcctt ttgtaattct gaataatgca aaattgatag cagaaataat c-aaaaaata taatg-ttga tatagttcat acaagatcaa gagcaccgg- catggagttc atatctagca acaaaa-tgg acaaatgcta aa-tttttga ctacctttca tggggtttat aatatcccg- aatagtttta aaaaa-tatt ataatagcat aatgctaaaa ggcaagaaag ttgttgccgt gtctaatttt gtaaaacaac acttgctcga aaactataaa attgatgaag ataaaatagt agtaattgag cgtggagtga attgtgatta tttcgatccc gcaaatttaa cgccggaaaa acttgaaaaa tgttgtgaga aatatgatgt accgagtaac gtgccgataa tattaatgcc ttctagaatg acaagttgga aaggacatct tgttctagta gaagcattaa gtaaattaaa acatagagac ttttattgtt taatggtcgg tgatatatct agacatccca attttactaa tagagtaaaa gagcttatag ctaatttaaa acttcaaaat aaaattcaaa tttttggtaa tgattcggat ataataaacc tatatgggat ttccgatatt atcatttctg cctcaatcga gcctgaagct ttcgggcgta ctattataga agggcaggcg atgaagaagc ttgttattgc aactaatatc ggtggggcag tagagacaat aaataataat ataacc-ggt ttccacgtag aaccaaataa tgccgaagcg ttagcacag- aaaatcgatt attgtttctc gattttaggt actgatcttg ctaagaaaat ccaagaagcg gcaaggcata cggtaattaa taatttctct cttaatctga tgctaagaaa aaaccttgaa atctataaag aaatcttaaa aaactcccat aattaa
Expected Protein Sequence
MAHHHHHHMP SLKSSKRYNK YTILQVVPAL VSGGVERGTI EVAKYLKILG HTPIIISVGG TLVKELDKED ILHIEMNSNS KNPFVILNNA KLIAEIIKKY NVDIVHTRSR APAWSSYLAT KWTNAKFLTT FHGVYNIPNS FKKYYNSIML KGKKVVAVSN FVKQHLLENY KIDEDKIVVI ERGVNCDYFD PANLTPEKLE KCCEKYDVPS NVPIILMPSR MTSWKGHLVL VEALSKLKHR DFYCLMVGDI SRHPNFTNRV KELIANLKLQ NKIQIFGNDS DIINLYGISD IIISASIEPE AFGRTIIEGQ AMKKLVIATN IGGAVETINN NITGFHVEPN NAEALAQKID YCFSILGTDL AKKIQEAARH TVINNFSLNL MLRKNLEIYK EILKNSHN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcctt cattaaagtc aagtaaacga tacaataaat atactatttt gcaagtagtg cctgctctag tttcgggagg ggttgaaaga ggtactatag aagttgcaaa atatcttaaa attctcggtc atactcctat cataatttca gtaggcggta ctctagttaa agagttagat aaggaagata tattgcatat tgaaatgaat agtaacagta aaaatccttt tgtaattctg aataatgcaa aattgatagc agaaataatc aaaaaatata atgttgatat agttcataca agatcaagag caccggcatg gagttcatat ctagcaacaa aatggacaaa tgctaaattt ttgactacct ttcatggggt ttataatatc ccgaatagtt ttaaaaaata ttataatagc ataatgctaa aaggcaagaa agttgttgcc gtgtctaatt ttgtaaaaca acacttgctc gaaaactata aaattgatga agataaaata gtagtaattg agcgtggagt gaattgtgat tatttcgatc ccgcaaattt aacgccggaa aaacttgaaa aatgttgtga gaaatatgat gtaccgagta acgtgccgat aatattaatg ccttctagaa tgacaagttg gaaaggacat cttgttctag tagaagcatt aagtaaatta aaacatagag acttttattg tttaatggtc ggtgatatat ctagacatcc caattttact aatagagtaa aagagcttat agctaattta aaacttcaaa ataaaattca aatttttggt aatgattcgg atataataaa cctatatggg atttccgata ttatcatttc tgcctcaatc gagcctgaag ctttcgggcg tactattata gaagggcagg cgatgaagaa gcttgttatt gcaactaata tcggtggggc agtagagaca ataaataata atataaccgg tttccatgta gaaccaaata atgccgaagc gttagcacag aaaatcgatt attgtttctc gattttaggt actgatcttg ctaagaaaat ccaagaagcg gcaaggcata cggtaattaa taatttctct cttaatctga tgctaagaaa aaaccttgaa atctataaag aaatcttaaa aaactcccat aattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for RiafA.17295.a.B1.PW36512
| PURIFICATION DATe: | 12/10/2012 |
| CONCENTRATION: | 23.9mg/ml |
| OBSERVED MW: | 45kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS SLKSSKRYNK YTILQVVPAL VSGGVERGTI EVAKYLKILG HTPIIISVGG TLVKELDKED ILHIEMNSNS KNPFVILNNA KLIAEIIKKY NVDIVHTRSR APAWSSYLAT KWTNAKFLTT FHGVYNIPNS FKKYYNSIML KGKKVVAVSN FVKQHLLENY KIDEDKIVVI ERGVNCDYFD PANLTPEKLE KCCEKYDVPS NVPIILMPSR MTSWKGHLVL VEALSKLKHR DFYCLMVGDI SRHPNFTNRV KELIANLKLQ NKIQIFGNDS DIINLYGISD IIISASIEPE AFGRTIIEGQ AMKKLVIATN IGGAVETINN NITGFHVEPN NAEALAQKID YCFSILGTDL AKKIQEAARH TVINNFSLNL MLRKNLEIYK EILKNSHN
Validated NT Sequence
atggctcacc accaccacca ccatatgtct tcattaaagt caagtaaacg atacaataaa tatactattt tgcaagtagt gcctgctcta gtttcgggag gggttgaaag aggtactata gaagttgcaa aatatcttaa aattctcggt catactccta tcataatttc agtaggcggt actctagtta aagagttaga taaggaagat atattgcata ttgaaatgaa tagtaacagt aaaaatcctt ttgtaattct gaataatgca aaattgatag cagaaataat c-aaaaaata taatg-ttga tatagttcat acaagatcaa gagcaccgg- catggagttc atatctagca acaaaa-tgg acaaatgcta aa-tttttga ctacctttca tggggtttat aatatcccg- aatagtttta aaaaa-tatt ataatagcat aatgctaaaa ggcaagaaag ttgttgccgt gtctaatttt gtaaaacaac acttgctcga aaactataaa attgatgaag ataaaatagt agtaattgag cgtggagtga attgtgatta tttcgatccc gcaaatttaa cgccggaaaa acttgaaaaa tgttgtgaga aatatgatgt accgagtaac gtgccgataa tattaatgcc ttctagaatg acaagttgga aaggacatct tgttctagta gaagcattaa gtaaattaaa acatagagac ttttattgtt taatggtcgg tgatatatct agacatccca attttactaa tagagtaaaa gagcttatag ctaatttaaa acttcaaaat aaaattcaaa tttttggtaa tgattcggat ataataaacc tatatgggat ttccgatatt atcatttctg cctcaatcga gcctgaagct ttcgggcgta ctattataga agggcaggcg atgaagaagc ttgttattgc aactaatatc ggtggggcag tagagacaat aaataataat ataacc-ggt ttccacgtag aaccaaataa tgccgaagcg ttagcacag- aaaatcgatt attgtttctc gattttaggt actgatcttg ctaagaaaat ccaagaagcg gcaaggcata cggtaattaa taatttctct cttaatctga tgctaagaaa aaaccttgaa atctataaag aaatcttaaa aaactcccat aattaa
Expressed Protein Sequence
MAHHHHHHMP SLKSSKRYNK YTILQVVPAL VSGGVERGTI EVAKYLKILG HTPIIISVGG TLVKELDKED ILHIEMNSNS KNPFVILNNA KLIAEIIKKY NVDIVHTRSR APAWSSYLAT KWTNAKFLTT FHGVYNIPNS FKKYYNSIML KGKKVVAVSN FVKQHLLENY KIDEDKIVVI ERGVNCDYFD PANLTPEKLE KCCEKYDVPS NVPIILMPSR MTSWKGHLVL VEALSKLKHR DFYCLMVGDI SRHPNFTNRV KELIANLKLQ NKIQIFGNDS DIINLYGISD IIISASIEPE AFGRTIIEGQ AMKKLVIATN IGGAVETINN NITGFHVEPN NAEALAQKID YCFSILGTDL AKKIQEAARH TVINNFSLNL MLRKNLEIYK EILKNSHN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCTT CATTAAAGTC AAGTAAACGA TACAATAAAT ATACTATTTT GCAAGTAGTG CCTGCTCTAG TTTCGGGAGG GGTTGAAAGA GGTACTATAG AAGTTGCAAA ATATCTTAAA ATTCTCGGTC ATACTCCTAT CATAATTTCA GTAGGCGGTA CTCTAGTTAA AGAGTTAGAT AAGGAAGATA TATTGCATAT TGAAATGAAT AGTAACAGTA AAAATCCTTT TGTAATTCTG AATAATGCAA AATTGATAGC AGAAATAATC AAAAAATATA ATGTTGATAT AGTTCATACA AGATCAAGAG CACCGGCATG GAGTTCATAT CTAGCAACAA AATGGACAAA TGCTAAATTT TTGACTACCT TTCATGGGGT TTATAATATC CCGAATAGTT TTAAAAAATA TTATAATAGC ATAATGCTAA AAGGCAAGAA AGTTGTTGCC GTGTCTAATT TTGTAAAACA ACACTTGCTC GAAAACTATA AAATTGATGA AGATAAAATA GTAGTAATTG AGCGTGGAGT GAATTGTGAT TATTTCGATC CCGCAAATTT AACGCCGGAA AAACTTGAAA AATGTTGTGA GAAATATGAT GTACCGAGTA ACGTGCCGAT AATATTAATG CCTTCTAGAA TGACAAGTTG GAAAGGACAT CTTGTTCTAG TAGAAGCATT AAGTAAATTA AAACATAGAG ACTTTTATTG TTTAATGGTC GGTGATATAT CTAGACATCC CAATTTTACT AATAGAGTAA AAGAGCTTAT AGCTAATTTA AAACTTCAAA ATAAAATTCA AATTTTTGGT AATGATTCGG ATATAATAAA CCTATATGGG ATTTCCGATA TTATCATTTC TGCCTCAATC GAGCCTGAAG CTTTCGGGCG TACTATTATA GAAGGGCAGG CGATGAAGAA GCTTGTTATT GCAACTAATA TCGGTGGGGC AGTAGAGACA ATAAATAATA ATATAACCGG TTTCCATGTA GAACCAAATA ATGCCGAAGC GTTAGCACAG AAAATCGATT ATTGTTTCTC GATTTTAGGT ACTGATCTTG CTAAGAAAAT CCAAGAAGCG GCAAGGCATA CGGTAATTAA TAATTTCTCT CTTAATCTGA TGCTAAGAAA AAACCTTGAA ATCTATAAAG AAATCTTAAA AAACTCCCAT AATTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT