SSGCID: Target Page

RiafA.10478.a

Branched-chain amino acid aminotransferase

CENTER ID:	RiafA.10478.a
ORGANISM:	Rickettsia africae ESF-5
ASSOCIATED DISEASE:	African tick-bite fever
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiafA.10478.a.B1.GE35843	Full length( RiafA.10478.a )		1	290

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|347255.3.peg.668
RefSeq:	YP_002845207.1
UniProt:	C3PNF4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTMVKLEQIF GHVWINGDLV PYQFARIHVL THSLHYSGSV FEGERAYNGK VFKLKEHTAR LIKSAEALGL KVPYNVDEII KAHERVIKQN NIKDAYIRPL IWCGDESLNI TNQYLSTNLL IAGIPSMPRS FEKGINLHVS RWRKAMPDST PVQSKSAAQY NMAITSKKEA KALGYEDALL LDYEGYIAEC TTTNIFFVKD KILYTPIADR FLNGITRQTI IEIAKDLGLE VKEERLKLEQ IEDFTGCFVT GTAIEVQNID SIDLGNKKII FDDHKIADRL KEEYRRVVRE

NT Sequence

ATGACAATGG TAAAACTAGA GCAAATTTTT GGGCATGTTT GGATTAACGG TGATTTAGTT CCTTATCAAT TTGCAAGGAT TCATGTTTTG ACTCATAGCC TGCATTATTC AGGATCGGTA TTTGAGGGTG AGAGAGCTTA TAACGGCAAA GTTTTTAAAC TAAAAGAACA TACGGCAAGA TTAATAAAAT CAGCAGAAGC ATTAGGTTTG AAAGTACCTT ATAATGTAGA CGAGATAATA AAGGCTCATG AACGTGTAAT AAAACAAAAT AATATTAAGG ATGCATATAT AAGACCGCTT ATTTGGTGCG GAGACGAGTC GCTAAATATC ACTAATCAGT ATTTATCTAC TAATCTTTTG ATTGCCGGTA TTCCATCTAT GCCGAGGTCT TTTGAGAAAG GTATAAATTT GCATGTAAGC CGCTGGCGTA AAGCAATGCC TGATAGCACC CCGGTACAAT CTAAATCCGC CGCTCAATAT AATATGGCAA TAACGAGTAA AAAAGAAGCT AAGGCTCTTG GCTATGAGGA TGCATTATTA CTTGATTATG AAGGGTATAT TGCTGAATGC ACTACAACAA ATATTTTCTT TGTTAAAGAT AAAATATTAT ATACTCCAAT TGCCGATAGG TTTTTAAACG GTATAACAAG ACAGACTATT ATTGAAATTG CAAAAGATTT AGGTCTAGAA GTAAAAGAAG AGCGTTTAAA ATTAGAGCAA ATAGAGGATT TTACCGGATG TTTTGTTACA GGCACAGCAA TTGAGGTACA AAATATTGAT TCTATAGATC TTGGCAATAA AAAGATTATT TTTGATGATC ATAAAATTGC AGATCGTTTG AAAGAAGAGT ATAGGCGTGT TGTTAGGGAG

Details for RiafA.10478.a.B1.GE35843

HARVESTED ON:	8/14/2012
SEQUENCED ON:	8/18/2012
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

cctatcttac tcacttactc cctaacaaca cgcctatact cttctttcaa acgatctgca attttatgat catcaaaaat aatcttttta ttgccaagat ctatagaatc aatattttgt acctcaattg ctgtgcctgt aacaaaacat ccggtaaaat cctctatttg ctctaatttt aaacgctctt cttttacttc tagacctaaa tcttttgcaa tttcaataat agtctgtctt gttataccgt ttaaaaacct atcggcaatt ggagtatata atattttatc tttaacaaag aaaatatttg ttgtagtgca ttcagcaata tacccttcat aatcaagtaa taatgcatcc tcatagccaa gagccttagc ttctttttta ctcgttattg ccatattata ttgagcggcg gatttagatt gtaccggggt gctatcaggc attgctttac gccagcggct tacatgcaaa tttatacctt tctcaaaaga cctcggcata gatggaatac cggcaatcaa aagattagta gataaatact gattagtgat atttagcgac tcgtctccgc accaaataag cggtcttata tatgcatcct taatattatt ttgttttatt acacgttcat gagcctttat tatctcgtct acattataag gtactttcaa acctaatgct tctgctgatt ttattaatct tgccgtatgt tcttttagtt taaaaacttt gccgttataa gctctctcac cctcaaatac cgatcctgaa taatgcaggc tatgagtcaa aacatgaatc cttgcaaatt gataaggaac taaatcaccg ttaatccaaa catgcccaaa aatttgctct agttttacca ttgtcatatg gtggtggtgg tggtgagcca

Expected Protein Sequence

MAHHHHHHMT MVKLEQIFGH VWINGDLVPY QFARIHVLTH SLHYSGSVFE GERAYNGKVF KLKEHTARLI KSAEALGLKV PYNVDEIIKA HERVIKQNNI KDAYIRPLIW CGDESLNITN QYLSTNLLIA GIPSMPRSFE KGINLHVSRW RKAMPDSTPV QSKSAAQYNM AITSKKEAKA LGYEDALLLD YEGYIAECTT TNIFFVKDKI LYTPIADRFL NGITRQTIIE IAKDLGLEVK EERLKLEQIE DFTGCFVTGT AIEVQNIDSI DLGNKKIIFD DHKIADRLKE EYRRVVRE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacaa tggtaaaact agagcaaatt tttgggcatg tttggattaa cggtgattta gttccttatc aatttgcaag gattcatgtt ttgactcata gcctgcatta ttcaggatcg gtatttgagg gtgagagagc ttataacggc aaagttttta aactaaaaga acatacggca agattaataa aatcagcaga agcattaggt ttgaaagtac cttataatgt agacgagata ataaaggctc atgaacgtgt aataaaacaa aataatatta aggatgcata tataagaccg cttatttggt gcggagacga gtcgctaaat atcactaatc agtatttatc tactaatctt ttgattgccg gtattccatc tatgccgagg tcttttgaga aaggtataaa tttgcatgta agccgctggc gtaaagcaat gcctgatagc accccggtac aatctaaatc cgccgctcaa tataatatgg caataacgag taaaaaagaa gctaaggctc ttggctatga ggatgcatta ttacttgatt atgaagggta tattgctgaa tgcactacaa caaatatttt ctttgttaaa gataaaatat tatatactcc aattgccgat aggtttttaa acggtataac aagacagact attattgaaa ttgcaaaaga tttaggtcta gaagtaaaag aagagcgttt aaaattagag caaatagagg attttaccgg atgttttgtt acaggcacag caattgaggt acaaaatatt gattctatag atcttggcaa taaaaagatt atttttgatg atcataaaat tgcagatcgt ttgaaagaag agtataggcg tgttgttagg gagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat