RiafA.10100.a

Periplasmic serine protease

CENTER ID: RiafA.10100.a
ORGANISM: Rickettsia africae ESF-5
ASSOCIATED DISEASE: African tick-bite fever
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiafA.10100.a.B2.GE35841 Mature protein( RiafA.10100.a ) 21 492
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiafA.10100.a.B2.PW37234 Mature protein( RiafA.10100.a ) 21 492

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|347255.3.peg.182
RefSeq: YP_002844865.1
UniProt: C3PMG2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVNLKIVIVI IVLISSNVVL AKENSRSLKV AAQEENEFTE INSAPLKVSE AARYSFADIV EPLIPAVVNI STIEYVNSKS ENAEKDPLQE KVNDFLEKLN IPLNLEEVDQ TPKSVPLGSG FIIEPNGLIV TNYHVIANVD KINIKLADNT ELSAKLIGND TKTDLALLKI DSEEPLPFVE FGDSNDARVG DWVIAIGNPF GNLGGTVTSG IISSKGRDID IDTDNIVDNF IQTDAAINNG NSGGPMFNLD QKVIGVNTAI FSPLGTNIGI GFAIPSNTAK PIIERLKKDG KVSRGRLGVT IQDLTEEISE GLGLKNTRGV LVAKVQEDGP GDKAGIKTGD IIIEFADIPV KNTKKLRVII ADAPIDQEVK VKILRDKKEL ELPIKITSDN EEVTKDSTEE TNKKEITNKE ENNLSITKNN ITFGNLTEEL RQKYTIPQDK MGIVITNINQ KSIDDISKLE ELYENAKKSD KQNILLLIER GSSNMFVPLQ VM
NT Sequence
ATGGTTAATC TGAAAATAGT TATTGTTATA ATAGTTTTAA TATCAAGTAA CGTTGTTCTA GCAAAAGAAA ATAGTAGGTC TTTAAAAGTA GCAGCTCAAG AAGAGAATGA ATTTACGGAA ATAAATTCTG CTCCTTTAAA AGTAAGTGAA GCCGCTCGTT ATAGCTTTGC CGATATAGTT GAACCGTTAA TTCCTGCAGT CGTTAATATT TCAACAATAG AATATGTTAA TAGTAAATCG GAGAACGCCG AGAAAGATCC TCTGCAAGAA AAAGTTAATG ATTTTTTAGA AAAGCTTAAT ATACCGCTGA ATTTGGAAGA GGTTGATCAA ACTCCGAAAA GTGTCCCGCT TGGTTCAGGA TTTATTATTG AACCTAACGG CTTAATAGTG ACAAACTATC ATGTAATTGC AAATGTTGAT AAAATTAATA TAAAACTTGC AGATAATACC GAATTATCGG CTAAATTAAT AGGTAACGAT ACTAAAACCG ATTTAGCTCT CTTAAAAATA GATAGCGAGG AACCTCTACC TTTTGTTGAG TTTGGAGATT CAAATGATGC AAGAGTAGGA GATTGGGTTA TTGCGATCGG TAATCCGTTC GGTAATCTAG GTGGTACGGT GACAAGTGGT ATTATTTCTT CTAAAGGGCG GGATATCGAT ATAGATACGG ACAATATAGT CGATAATTTT ATTCAAACGG ATGCTGCAAT TAATAACGGT AATTCCGGTG GTCCTATGTT TAATTTGGAT CAGAAAGTAA TCGGCGTAAA TACGGCAATT TTTTCACCGC TCGGTACTAA TATAGGTATC GGTTTTGCAA TACCGTCAAA TACTGCAAAG CCTATAATCG AACGTCTTAA GAAAGATGGA AAAGTAAGTA GAGGGCGCCT TGGAGTAACA ATACAAGATT TAACCGAGGA AATTTCTGAA GGGCTAGGAC TTAAAAATAC TAGAGGAGTG TTAGTAGCTA AAGTACAAGA AGACGGTCCA GGTGATAAAG CGGGAATTAA AACAGGTGAT ATAATAATAG AGTTTGCAGA TATACCGGTT AAAAATACTA AAAAATTACG TGTAATTATT GCAGATGCTC CTATTGATCA GGAAGTAAAA GTAAAAATAC TTCGCGATAA AAAGGAGCTT GAGTTACCTA TTAAAATTAC TTCGGATAAT GAAGAGGTTA CCAAAGATTC TACAGAAGAG ACCAATAAGA AAGAAATAAC AAATAAAGAA GAAAATAATT TATCTATTAC TAAAAATAAT ATTACTTTTG GTAATTTGAC TGAAGAACTA AGACAAAAAT ATACTATTCC TCAAGATAAA ATGGGAATAG TGATAACCAA TATTAATCAG AAAAGCATAG ACGATATAAG TAAGCTAGAA GAATTATATG AAAATGCTAA AAAATCAGAT AAGCAAAATA TTTTGCTCTT GATTGAAAGG GGGAGTAGTA ATATGTTCGT ACCATTGCAG GTTATG
Details for RiafA.10100.a.B2.GE35841
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 49
Validated AA Sequence
MFNLDQKVIG VNTAIFSPLG TNIGIGFAIP SNTAKPIIER LKKDGKVSRG RLGVTIQDLT EEISEGLGLK NTRGVLVAKV QEDGPGDKAG IKTGDIIIEF ADIPVKNTKK LRVIIADAPI DQEVKVKILR DKKELELPIK ITSDNEEVTK DSTEETNKKE ITNKEENNLS ITKNNITFGN LTEELRQKYT IPQDKMGIVI TNINQKSIDD ISKLEELYEN AKKSDKQNIL LLIERGSS
Validated NT Sequence
attactactc cccctttcaa tcaagagcaa aatattttgc ttatctgatt ttttagcatt ttcatataat tcttctagct tacttatatc gtctatgctt ttctgattaa tattggttat cactattccc attttatctt gaggaatagt atatttttgt cttagttctt cagtcaaatt accaaaagta atattatttt tagtaataga taaattattt tcttctttat ttgttatttc tttcttattg gtctcttctg tagaatcttt ggtaacctct tcattatccg aagtaatttt aataggtaac tcaagctcct ttttatcgcg aagtattttt acttttactt cctgatcaat aggagcatct gcaataatta cacgtaattt tttagtattt ttaaccggta tatctgcaaa ctctattatt atatcacctg ttttaattcc cgctttatca cctggaccgt cttcttgtac tttagctact aacactcctc tagtattttt aagtcctagc ccttcagaaa tttcctcggt taaatcttgt attgttactc caaggcgccc tctacttact tttccatctt tcttaagacg ttcgattata ggctttgcag tatttgacgg tattgcaaaa ccgataccta tattagtacc gagcggtgaa aaaattgccg tatttacgcc gattactttc tgatccaaat taaacatagg accaccggaa ttaccgttat taattgcagc atccgtttga ataaaattat cgactatatt gtccgtatct atatcgatat cccgcccttt agaagaaata ataccacttg tcaccgtacc acctagatta ccgaacggat taccgatcgc aataacccaa tctcctactc ttgcatcatt tgaatctcca aactcaacaa aaggtagagg ttcctcgcta tctattttta agagagctaa atcggtttta gtatcgttac ctattaattt agccgataat tcggtattat ctgcaagttt tatattaatt ttatcaacat ttgcaattac atgatagttt gtcactatta agccgttagg ttcaataata aatcctgaac caagcgggac acttttcgga gtttgatcaa cctcttccaa attcagcggt atattaagct tttctaaaaa atcattaact ttttcttgca gaggatcttt ctcggcgttc tccgatttac tattaacata ttctattgtt gaaatattaa cgactgcagg aattaacggt tcaactatat cggcaaagct ataacgagcg gcttcactta cttttaaagg agcagaattt atttccgtaa attcattctc ttcttgagct gctactttta aagacctact attt
Expected Protein Sequence
MAHHHHHHMA KENSRSLKVA AQEENEFTEI NSAPLKVSEA ARYSFADIVE PLIPAVVNIS TIEYVNSKSE NAEKDPLQEK VNDFLEKLNI PLNLEEVDQT PKSVPLGSGF IIEPNGLIVT NYHVIANVDK INIKLADNTE LSAKLIGNDT KTDLALLKID SEEPLPFVEF GDSNDARVGD WVIAIGNPFG NLGGTVTSGI ISSKGRDIDI DTDNIVDNFI QTDAAINNGN SGGPMFNLDQ KVIGVNTAIF SPLGTNIGIG FAIPSNTAKP IIERLKKDGK VSRGRLGVTI QDLTEEISEG LGLKNTRGVL VAKVQEDGPG DKAGIKTGDI IIEFADIPVK NTKKLRVIIA DAPIDQEVKV KILRDKKELE LPIKITSDNE EVTKDSTEET NKKEITNKEE NNLSITKNNI TFGNLTEELR QKYTIPQDKM GIVITNINQK SIDDISKLEE LYENAKKSDK QNILLLIERG SSNMFVPLQV M
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa aagaaaatag taggtcttta aaagtagcag ctcaagaaga gaatgaattt acggaaataa attctgctcc tttaaaagta agtgaagccg ctcgttatag ctttgccgat atagttgaac cgttaattcc tgcagtcgtt aatatttcaa caatagaata tgttaatagt aaatcggaga acgccgagaa agatcctctg caagaaaaag ttaatgattt tttagaaaag cttaatatac cgctgaattt ggaagaggtt gatcaaactc cgaaaagtgt cccgcttggt tcaggattta ttattgaacc taacggctta atagtgacaa actatcatgt aattgcaaat gttgataaaa ttaatataaa acttgcagat aataccgaat tatcggctaa attaataggt aacgatacta aaaccgattt agctctctta aaaatagata gcgaggaacc tctacctttt gttgagtttg gagattcaaa tgatgcaaga gtaggagatt gggttattgc gatcggtaat ccgttcggta atctaggtgg tacggtgaca agtggtatta tttcttctaa agggcgggat atcgatatag atacggacaa tatagtcgat aattttattc aaacggatgc tgcaattaat aacggtaatt ccggtggtcc tatgtttaat ttggatcaga aagtaatcgg cgtaaatacg gcaatttttt caccgctcgg tactaatata ggtatcggtt ttgcaatacc gtcaaatact gcaaagccta taatcgaacg tcttaagaaa gatggaaaag taagtagagg gcgccttgga gtaacaatac aagatttaac cgaggaaatt tctgaagggc taggacttaa aaatactaga ggagtgttag tagctaaagt acaagaagac ggtccaggtg ataaagcggg aattaaaaca ggtgatataa taatagagtt tgcagatata ccggttaaaa atactaaaaa attacgtgta attattgcag atgctcctat tgatcaggaa gtaaaagtaa aaatacttcg cgataaaaag gagcttgagt tacctattaa aattacttcg gataatgaag aggttaccaa agattctaca gaagagacca ataagaaaga aataacaaat aaagaagaaa ataatttatc tattactaaa aataatatta cttttggtaa tttgactgaa gaactaagac aaaaatatac tattcctcaa gataaaatgg gaatagtgat aaccaatatt aatcagaaaa gcatagacga tataagtaag ctagaagaat tatatgaaaa tgctaaaaaa tcagataagc aaaatatttt gctcttgatt gaaaggggga gtagtaatat gttcgtacca ttgcaggtta tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for RiafA.10100.a.B2.PW37234
PURIFICATION DATe: 1/14/2013
CONCENTRATION: 26.6mg/ml
OBSERVED MW: 56kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 49
Protocol Notes
notes unavailable
Validated AA Sequence
MFNLDQKVIG VNTAIFSPLG TNIGIGFAIP SNTAKPIIER LKKDGKVSRG RLGVTIQDLT EEISEGLGLK NTRGVLVAKV QEDGPGDKAG IKTGDIIIEF ADIPVKNTKK LRVIIADAPI DQEVKVKILR DKKELELPIK ITSDNEEVTK DSTEETNKKE ITNKEENNLS ITKNNITFGN LTEELRQKYT IPQDKMGIVI TNINQKSIDD ISKLEELYEN AKKSDKQNIL LLIERGSS
Validated NT Sequence
attactactc cccctttcaa tcaagagcaa aatattttgc ttatctgatt ttttagcatt ttcatataat tcttctagct tacttatatc gtctatgctt ttctgattaa tattggttat cactattccc attttatctt gaggaatagt atatttttgt cttagttctt cagtcaaatt accaaaagta atattatttt tagtaataga taaattattt tcttctttat ttgttatttc tttcttattg gtctcttctg tagaatcttt ggtaacctct tcattatccg aagtaatttt aataggtaac tcaagctcct ttttatcgcg aagtattttt acttttactt cctgatcaat aggagcatct gcaataatta cacgtaattt tttagtattt ttaaccggta tatctgcaaa ctctattatt atatcacctg ttttaattcc cgctttatca cctggaccgt cttcttgtac tttagctact aacactcctc tagtattttt aagtcctagc ccttcagaaa tttcctcggt taaatcttgt attgttactc caaggcgccc tctacttact tttccatctt tcttaagacg ttcgattata ggctttgcag tatttgacgg tattgcaaaa ccgataccta tattagtacc gagcggtgaa aaaattgccg tatttacgcc gattactttc tgatccaaat taaacatagg accaccggaa ttaccgttat taattgcagc atccgtttga ataaaattat cgactatatt gtccgtatct atatcgatat cccgcccttt agaagaaata ataccacttg tcaccgtacc acctagatta ccgaacggat taccgatcgc aataacccaa tctcctactc ttgcatcatt tgaatctcca aactcaacaa aaggtagagg ttcctcgcta tctattttta agagagctaa atcggtttta gtatcgttac ctattaattt agccgataat tcggtattat ctgcaagttt tatattaatt ttatcaacat ttgcaattac atgatagttt gtcactatta agccgttagg ttcaataata aatcctgaac caagcgggac acttttcgga gtttgatcaa cctcttccaa attcagcggt atattaagct tttctaaaaa atcattaact ttttcttgca gaggatcttt ctcggcgttc tccgatttac tattaacata ttctattgtt gaaatattaa cgactgcagg aattaacggt tcaactatat cggcaaagct ataacgagcg gcttcactta cttttaaagg agcagaattt atttccgtaa attcattctc ttcttgagct gctactttta aagacctact attt
Expressed Protein Sequence
MAHHHHHHMA KENSRSLKVA AQEENEFTEI NSAPLKVSEA ARYSFADIVE PLIPAVVNIS TIEYVNSKSE NAEKDPLQEK VNDFLEKLNI PLNLEEVDQT PKSVPLGSGF IIEPNGLIVT NYHVIANVDK INIKLADNTE LSAKLIGNDT KTDLALLKID SEEPLPFVEF GDSNDARVGD WVIAIGNPFG NLGGTVTSGI ISSKGRDIDI DTDNIVDNFI QTDAAINNGN SGGPMFNLDQ KVIGVNTAIF SPLGTNIGIG FAIPSNTAKP IIERLKKDGK VSRGRLGVTI QDLTEEISEG LGLKNTRGVL VAKVQEDGPG DKAGIKTGDI IIEFADIPVK NTKKLRVIIA DAPIDQEVKV KILRDKKELE LPIKITSDNE EVTKDSTEET NKKEITNKEE NNLSITKNNI TFGNLTEELR QKYTIPQDKM GIVITNINQK SIDDISKLEE LYENAKKSDK QNILLLIERG SSNMFVPLQV M
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAA AAGAAAATAG TAGGTCTTTA AAAGTAGCAG CTCAAGAAGA GAATGAATTT ACGGAAATAA ATTCTGCTCC TTTAAAAGTA AGTGAAGCCG CTCGTTATAG CTTTGCCGAT ATAGTTGAAC CGTTAATTCC TGCAGTCGTT AATATTTCAA CAATAGAATA TGTTAATAGT AAATCGGAGA ACGCCGAGAA AGATCCTCTG CAAGAAAAAG TTAATGATTT TTTAGAAAAG CTTAATATAC CGCTGAATTT GGAAGAGGTT GATCAAACTC CGAAAAGTGT CCCGCTTGGT TCAGGATTTA TTATTGAACC TAACGGCTTA ATAGTGACAA ACTATCATGT AATTGCAAAT GTTGATAAAA TTAATATAAA ACTTGCAGAT AATACCGAAT TATCGGCTAA ATTAATAGGT AACGATACTA AAACCGATTT AGCTCTCTTA AAAATAGATA GCGAGGAACC TCTACCTTTT GTTGAGTTTG GAGATTCAAA TGATGCAAGA GTAGGAGATT GGGTTATTGC GATCGGTAAT CCGTTCGGTA ATCTAGGTGG TACGGTGACA AGTGGTATTA TTTCTTCTAA AGGGCGGGAT ATCGATATAG ATACGGACAA TATAGTCGAT AATTTTATTC AAACGGATGC TGCAATTAAT AACGGTAATT CCGGTGGTCC TATGTTTAAT TTGGATCAGA AAGTAATCGG CGTAAATACG GCAATTTTTT CACCGCTCGG TACTAATATA GGTATCGGTT TTGCAATACC GTCAAATACT GCAAAGCCTA TAATCGAACG TCTTAAGAAA GATGGAAAAG TAAGTAGAGG GCGCCTTGGA GTAACAATAC AAGATTTAAC CGAGGAAATT TCTGAAGGGC TAGGACTTAA AAATACTAGA GGAGTGTTAG TAGCTAAAGT ACAAGAAGAC GGTCCAGGTG ATAAAGCGGG AATTAAAACA GGTGATATAA TAATAGAGTT TGCAGATATA CCGGTTAAAA ATACTAAAAA ATTACGTGTA ATTATTGCAG ATGCTCCTAT TGATCAGGAA GTAAAAGTAA AAATACTTCG CGATAAAAAG GAGCTTGAGT TACCTATTAA AATTACTTCG GATAATGAAG AGGTTACCAA AGATTCTACA GAAGAGACCA ATAAGAAAGA AATAACAAAT AAAGAAGAAA ATAATTTATC TATTACTAAA AATAATATTA CTTTTGGTAA TTTGACTGAA GAACTAAGAC AAAAATATAC TATTCCTCAA GATAAAATGG GAATAGTGAT AACCAATATT AATCAGAAAA GCATAGACGA TATAAGTAAG CTAGAAGAAT TATATGAAAA TGCTAAAAAA TCAGATAAGC AAAATATTTT GCTCTTGATT GAAAGGGGGA GTAGTAATAT GTTCGTACCA TTGCAGGTTA TGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT