RiafA.10014.f

ABC transporter ATP-binding protein

CENTER ID: RiafA.10014.f
ORGANISM: Rickettsia africae ESF-5
ASSOCIATED DISEASE: African tick-bite fever
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RiafA.10014.f.B1.GE35840 Full length( RiafA.10014.f ) 1 240
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|347255.3.peg.562
RefSeq: YP_002845136.1
UniProt: C3PN83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKPYLYAENI QFKVKERTEP IIAETTLNIT KEEFVVILGH NGSGKSTLAK ILAGYLKPTS GKVFLDQVRI DKIPKGQKAS TLVTLTQKAE ERLFTELTLE ENVILWESRF SSNERLASSE LLELTGAPKR FLPLLSQPLG KFSGGEKQMI LLALIIAHPP KILFLDEHTA NLDPKASLEV MKKTAEIIEK HKITSVMITH NLEDAVNYGK RLIVLDSGKV VLDYLKPPGF SLKELKEILN
NT Sequence
ATGAAACCTT ATTTATATGC AGAAAATATA CAGTTCAAAG TTAAAGAAAG AACCGAACCT ATAATTGCTG AAACTACTTT AAATATTACT AAAGAAGAGT TTGTTGTAAT ATTGGGTCAT AACGGTAGCG GTAAGTCTAC TTTAGCTAAA ATACTTGCCG GCTATTTAAA ACCTACTAGC GGGAAAGTGT TTCTAGATCA AGTCAGAATC GATAAAATAC CTAAAGGACA AAAAGCTTCT ACATTAGTTA CATTAACACA AAAAGCTGAA GAGAGGCTAT TTACCGAGCT AACCCTTGAA GAAAATGTCA TTTTGTGGGA AAGTAGATTC TCTAGTAATG AACGGTTAGC AAGTAGCGAG TTGTTAGAGC TTACAGGTGC GCCTAAACGC TTTTTGCCTT TACTTTCACA GCCATTAGGT AAATTTTCCG GTGGAGAAAA ACAGATGATA CTGCTGGCAC TTATCATAGC TCATCCACCT AAAATATTAT TTCTAGACGA ACATACGGCA AATTTAGATC CGAAAGCTTC TTTGGAAGTG ATGAAAAAAA CAGCAGAGAT TATAGAGAAA CATAAGATTA CTTCTGTTAT GATCACGCAT AATTTAGAAG ATGCCGTAAA TTACGGCAAA AGGCTTATAG TCTTAGATAG TGGTAAGGTA GTACTAGACT ACCTAAAACC ACCGGGTTTT TCTTTAAAAG AACTTAAAGA GATTTTAAAT
Details for RiafA.10014.f.B1.GE35840
HARVESTED ON: 8/14/2012
SEQUENCED ON: 8/18/2012
EXPECTED MW: 27kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK PYLYAENIQF KVKERTEPII AETTLNITKE EFVVILGHNG SGKSTLAKIL VGYLKPTSGK VFLDQVRIDK IPKGQKASTL VTLTQKAEER LFTELTLEEN VILWESRFSS NERLASSELL ELTGAPKRFL PLLSQPLGKF SGGEKQMILL ALIIAHPPKI LFLDEHTANL DPKASLEVMK KTAEIIEKHK ITSVMITHNL EDAVNYGKRL IVLDSGKVVL DYLKPPGFSL KELKEILN
Validated NT Sequence
attttgntta ctttaagaag gaganatacc atggctcacc accaccacca ccatatgaaa ccttatttat atgcagaaaa tatacagttc aaagttaaag aaagaaccga acctataatt gctgaaacta ctttaaatat tactaaagaa gagtttgttg taatattggg tcataacggt agcggtaagt ctactttagc taaaatactt gtcggctatt taaaacctac tagcgggaaa gtgtttctag atcaagtcag aatcgataaa atacctaaag gacaaaaagc ttctacatta gttacattaa cacaaaaagc tgaagagagg ctatttaccg agctaaccct tgaagaaaat gtcattttgt gggaaagtag attctctagt aatgaacggt tagcaagtag cgagttgtta gagcttacag gtgcgcctaa acgctttttg cctttacttt cacagccatt aggtaaattt tccggtggag aaaaacagat gatactgctg gcacttatca tagctcatcc acctaaaata ttatttctag acgaacatac ggcaaattta gatccgaaag cttctttgga agtgatgaaa aaaacagcag agattataga gaaacataag atcacttctg ttatgatcac gcataattta gaagatgccg taaattacgg caaaaggctt atagtcttag atagtggtaa ggtagtacta gactacctaa aaccaccggg tttttcttta aaagaactta aagagatttt aaattaagtg agtaagatag gatccggnnn nnnnnnnccc cc
Expected Protein Sequence
MAHHHHHHMK PYLYAENIQF KVKERTEPII AETTLNITKE EFVVILGHNG SGKSTLAKIL AGYLKPTSGK VFLDQVRIDK IPKGQKASTL VTLTQKAEER LFTELTLEEN VILWESRFSS NERLASSELL ELTGAPKRFL PLLSQPLGKF SGGEKQMILL ALIIAHPPKI LFLDEHTANL DPKASLEVMK KTAEIIEKHK ITSVMITHNL EDAVNYGKRL IVLDSGKVVL DYLKPPGFSL KELKEILN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac cttatttata tgcagaaaat atacagttca aagttaaaga aagaaccgaa cctataattg ctgaaactac tttaaatatt actaaagaag agtttgttgt aatattgggt cataacggta gcggtaagtc tactttagct aaaatacttg ccggctattt aaaacctact agcgggaaag tgtttctaga tcaagtcaga atcgataaaa tacctaaagg acaaaaagct tctacattag ttacattaac acaaaaagct gaagagaggc tatttaccga gctaaccctt gaagaaaatg tcattttgtg ggaaagtaga ttctctagta atgaacggtt agcaagtagc gagttgttag agcttacagg tgcgcctaaa cgctttttgc ctttactttc acagccatta ggtaaatttt ccggtggaga aaaacagatg atactgctgg cacttatcat agctcatcca cctaaaatat tatttctaga cgaacatacg gcaaatttag atccgaaagc ttctttggaa gtgatgaaaa aaacagcaga gattatagag aaacataaga ttacttctgt tatgatcacg cataatttag aagatgccgt aaattacggc aaaaggctta tagtcttaga tagtggtaag gtagtactag actacctaaa accaccgggt ttttctttaa aagaacttaa agagatttta aattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat