SSGCID: Target Page

RiafA.10014.d

O-antigen export system ATP-binding protein RfbE

CENTER ID:	RiafA.10014.d
ORGANISM:	Rickettsia africae ESF-5
ASSOCIATED DISEASE:	African tick-bite fever
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RiafA.10014.d.B1.GE35838	Full length( RiafA.10014.d )		1	249

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|347255.3.peg.3
RefSeq:	YP_002844734.1
UniProt:	C3PM31

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLSYSKVFI EITNGYVEGI DTHKRAQGLK HFFLKKGVSL SPNIPILNNI NFSCYEGEKI AFIGSNGSGK SSFLKLIAGI YPLKSGTVKV HGDIAAIIDM GVGFESEQTG RENIKMLMLY NNMLDKYSKK IEKEIIDFSE LGSKIDLPIK IYSSGMLSRL AFSVSIFQNP QILLLDEVFA AGDSYFIEKS LSLMKNKFKN TPISIIVSHQ EEIIKDNCNR CILLKDGNII GDGTPSEMFK IYNQQQGNS

NT Sequence

ATGTCACTTA GCTATTCAAA AGTATTTATA GAGATAACTA ATGGTTATGT TGAAGGTATT GATACTCATA AAAGAGCTCA AGGTTTAAAG CATTTCTTCT TGAAAAAAGG AGTCTCTCTT TCTCCAAATA TACCTATATT AAACAATATT AATTTTTCTT GTTATGAGGG AGAAAAAATA GCTTTTATAG GGAGTAACGG TTCGGGAAAA AGTTCGTTTC TAAAGTTAAT CGCCGGAATA TATCCGTTAA AATCCGGTAC AGTCAAAGTT CATGGAGATA TTGCGGCAAT TATAGATATG GGAGTTGGTT TTGAGTCTGA GCAAACTGGT CGTGAAAATA TAAAAATGTT AATGCTATAT AATAATATGC TGGATAAATA TAGCAAAAAA ATTGAAAAAG AAATTATAGA TTTTTCAGAA CTTGGTAGTA AAATTGATTT ACCGATAAAA ATTTATAGCT CCGGTATGTT ATCACGTCTT GCTTTTTCTG TATCGATATT TCAAAATCCG CAGATTCTAT TACTTGATGA AGTTTTTGCG GCAGGCGATA GCTATTTTAT AGAAAAATCT CTTAGTTTAA TGAAGAATAA ATTTAAAAAT ACTCCTATTT CAATAATAGT AAGCCATCAA GAAGAAATTA TAAAAGATAA TTGCAATAGA TGTATTTTAT TAAAAGACGG CAATATTATA GGTGATGGTA CACCATCGGA AATGTTTAAA ATCTATAACC AACAACAAGG AAATTCA

Details for RiafA.10014.d.B1.GE35838

HARVESTED ON:	8/14/2012
SEQUENCED ON:	8/18/2012
EXPECTED MW:	28kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS LSYSKVFIEI TNGYVEGIDT HKRAQGLKHF FLKKGVSLSP NIPILNNINF SCYEGEKIAF IGSNGSGKSS FLKLIAGIYP LKSGTVKVHG DIAAIIDMGV GFESEQTGRE NIKMLMLYNN MLDKYSKKIE KEIIDFSELG SKIDLPIKIY SSGMLSRLAF SVSIFQNPQI LLLDEVFAAG DSYFIEKSLS LMKNKFKNTP ISIIVSHQEK IIKDNCNRCI LLKDGNIIGD GTPSEMFKIY NQQQGNS

Validated NT Sequence

attttgttnn ntttaagaag gaganatacc atggctcacc accaccacca ccatatgtca cttagctatt caaaagtatt tatagagata actaatggtt atgttgaagg tattgatact cataaaagag ctcaaggttt aaagcatttc ttcttgaaaa aaggagtctc tctttctcca aatataccta tattaaacaa tattaatttt tcttgttatg agggagaaaa aatagctttt atagggagta acggttcggg aaaaagttcg tttctaaagt taatcgccgg aatatatccg ttaaaatccg gtacagtcaa agttcatgga gatattgcgg caattataga tatgggagtt ggttttgagt ctgagcaaac tggtcgtgaa aatataaaaa tgttaatgct atataataat atgctggata aatatagcaa aaaaattgaa aaagaaatta tagatttttc agaacttggt agtaaaattg atttaccgat aaaaatttat agctccggta tgttatcacg tcttgctttt tctgtatcga tatttcaaaa tccgcagatt ctattacttg atgaagtttt tgcggcaggc gatagctatt ttatagaaaa atctcttagt ttaatgaaga ataaatttaa aaatactcct atttcaataa tagtaagcca tcaagaaaaa attataaaag ataattgcaa tagatgtatt ttattaaaag acggcaatat tataggtgat ggtacaccat cggaaatgtt taaaatctat aaccaacaac aaggaaattc ataagtgagt aagataggat ccggntnnnn nnnccccnnn nnnannnnnn nnnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcac ttagctattc aaaagtattt atagagataa ctaatggtta tgttgaaggt attgatactc ataaaagagc tcaaggttta aagcatttct tcttgaaaaa aggagtctct ctttctccaa atatacctat attaaacaat attaattttt cttgttatga gggagaaaaa atagctttta tagggagtaa cggttcggga aaaagttcgt ttctaaagtt aatcgccgga atatatccgt taaaatccgg tacagtcaaa gttcatggag atattgcggc aattatagat atgggagttg gttttgagtc tgagcaaact ggtcgtgaaa atataaaaat gttaatgcta tataataata tgctggataa atatagcaaa aaaattgaaa aagaaattat agatttttca gaacttggta gtaaaattga tttaccgata aaaatttata gctccggtat gttatcacgt cttgcttttt ctgtatcgat atttcaaaat ccgcagattc tattacttga tgaagttttt gcggcaggcg atagctattt tatagaaaaa tctcttagtt taatgaagaa taaatttaaa aatactccta tttcaataat agtaagccat caagaagaaa ttataaaaga taattgcaat agatgtattt tattaaaaga cggcaatatt ataggtgatg gtacaccatc ggaaatgttt aaaatctata accaacaaca aggaaattca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat